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Q9NSA0 (S22AB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 11
Alternative name(s):
Organic anion transporter 4
Gene names
Name:SLC22A11
Synonyms:OAT4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates saturable uptake of estrone sulfate, dehydroepiandrosterone sulfate and related compounds. Ref.1 Ref.7 Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.7 Ref.9.

Tissue specificity

Detected in placenta and kidney. Ref.1

Post-translational modification

N-glycosylated. Contains several complex-type N-glycans. Ref.9

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Sequence caution

The sequence BAC11483.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NSA0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NSA0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-461: NFSLLISYYG...KAELFPTPVR → K
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550Solute carrier family 22 member 11
PRO_0000234435

Regions

Topological domain1 – 1010Cytoplasmic Potential
Transmembrane11 – 3121Helical; Potential
Topological domain32 – 142111Extracellular Potential
Transmembrane143 – 16321Helical; Potential
Topological domain164 – 17411Cytoplasmic Potential
Transmembrane175 – 19521Helical; Potential
Topological domain196 – 2005Extracellular Potential
Transmembrane201 – 22121Helical; Potential
Topological domain222 – 23110Cytoplasmic Potential
Transmembrane232 – 25221Helical; Potential
Topological domain253 – 2564Extracellular Potential
Transmembrane257 – 27721Helical; Potential
Topological domain278 – 34669Cytoplasmic Potential
Transmembrane347 – 36721Helical; Potential
Topological domain368 – 37811Extracellular Potential
Transmembrane379 – 39921Helical; Potential
Topological domain400 – 4023Cytoplasmic Potential
Transmembrane403 – 42321Helical; Potential
Topological domain424 – 4307Extracellular Potential
Transmembrane431 – 45121Helical; Potential
Topological domain452 – 46312Cytoplasmic Potential
Transmembrane464 – 48421Helical; Potential
Topological domain485 – 4906Extracellular Potential
Transmembrane491 – 51121Helical; Potential
Topological domain512 – 55039Cytoplasmic Potential

Amino acid modifications

Glycosylation391N-linked (GlcNAc...) Potential
Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence353 – 461109NFSLL…PTPVR → K in isoform 2.
VSP_018319
Natural variant1551V → G.
Corresponds to variant rs12785832 [ dbSNP | Ensembl ].
VAR_053962

Experimental info

Mutagenesis391N → Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface location; when associated with Q-56; Q-63 and Q-99. Ref.9
Mutagenesis471H → A: Reduced cell surface expression and estrone sulfate transport. Reduced cell surface expression and estrone sulfate transport; when associated with A-52; A-83; A-305 and A-469. Ref.7
Mutagenesis521H → A: Slightly reduced estrone sulfate transport. Reduced cell surface expression and estrone sulfate transport; when associated with A-47; A-83; A-305 and A-469. Ref.7
Mutagenesis561N → Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-63 and Q-99. Ref.9
Mutagenesis631N → Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-56 and Q-99. Ref.9
Mutagenesis831H → A: Reduced cell surface expression and estrone sulfate transport; when associated with A-47; A-52; A-305 and A-469. Ref.7
Mutagenesis991N → Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-56 and Q-63. Ref.9
Mutagenesis2411G → L, S or V: Strongly reduced cell surface expression and estrone sulfate transport. Ref.8
Mutagenesis3051H → A: Reduced cell surface expression and estrone sulfate transport; when associated with A-47; A-52; A-83 and A-469. Ref.7
Mutagenesis4001G → L, S or V: Strongly reduced cell surface expression and estrone sulfate transport. Ref.8
Mutagenesis4691H → A: Slightly reduced estrone sulfate transport. Reduced cell surface expression and estrone sulfate transport; when associated with A-47; A-52; A-83 and A-305. Ref.7
Sequence conflict1801L → S in BAD96589. Ref.3
Sequence conflict3761L → R in BAC85252. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 233BE6C4A520E58A

FASTA55059,972
        10         20         30         40         50         60 
MAFSKLLEQA GGVGLFQTLQ VLTFILPCLM IPSQMLLENF SAAIPGHRCW THMLDNGSAV 

        70         80         90        100        110        120 
STNMTPKALL TISIPPGPNQ GPHQCRRFRQ PQWQLLDPNA TATSWSEADT EPCVDGWVYD 

       130        140        150        160        170        180 
RSVFTSTIVA KWDLVCSSQG LKPLSQSIFM SGILVGSFIW GLLSYRFGRK PMLSWCCLQL 

       190        200        210        220        230        240 
AVAGTSTIFA PTFVIYCGLR FVAAFGMAGI FLSSLTLMVE WTTTSRRAVT MTVVGCAFSA 

       250        260        270        280        290        300 
GQAALGGLAF ALRDWRTLQL AASVPFFAIS LISWWLPESA RWLIIKGKPD QALQELRKVA 

       310        320        330        340        350        360 
RINGHKEAKN LTIEVLMSSV KEEVASAKEP RSVLDLFCVP VLRWRSCAML VVNFSLLISY 

       370        380        390        400        410        420 
YGLVFDLQSL GRDIFLLQAL FGAVDFLGRA TTALLLSFLG RRTIQAGSQA MAGLAILANM 

       430        440        450        460        470        480 
LVPQDLQTLR VVFAVLGKGC FGISLTCLTI YKAELFPTPV RMTADGILHT VGRLGAMMGP 

       490        500        510        520        530        540 
LILMSRQALP LLPPLLYGVI SIASSLVVLF FLPETQGLPL PDTIQDLESQ KSTAAQGNRQ 

       550 
EAVTVESTSL 

« Hide

Isoform 2 [UniParc].

Checksum: F9D42F38249733FB
Show »

FASTA44248,301

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of multispecific organic anion transporter 4 expressed in the placenta."
Cha S.H., Sekine T., Kusuhara H., Yu E., Kim J.Y., Kim D.K., Sugiyama Y., Kanai Y., Endou H.
J. Biol. Chem. 275:4507-4512(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Tissue: Kidney.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Kidney and Placenta.
[3]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Liver.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Colon.
[7]"Mutational analysis of histidine residues in human organic anion transporter 4 (hOAT4)."
Zhou F., Pan Z., Ma J., You G.
Biochem. J. 384:87-92(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF HIS-47; HIS-52; HIS-83; HIS-305 AND HIS-469, FUNCTION, SUBCELLULAR LOCATION.
[8]"The role of glycine residues in the function of human organic anion transporter 4."
Zhou F., Tanaka K., Pan Z., Ma J., You G.
Mol. Pharmacol. 65:1141-1147(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-241 AND GLY-400.
[9]"The role of N-linked glycosylation in protein folding, membrane targeting, and substrate binding of human organic anion transporter hOAT4."
Zhou F., Xu W., Hong M., Pan Z., Sinko P.J., Ma J., You G.
Mol. Pharmacol. 67:868-876(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASN-39; ASN-56; ASN-63 AND ASN-99, SUBCELLULAR LOCATION, GLYCOSYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026116 mRNA. Translation: BAA95316.1.
AK075224 mRNA. Translation: BAC11483.1. Different initiation.
AK129930 mRNA. Translation: BAC85252.1.
AK222869 mRNA. Translation: BAD96589.1.
AK290791 mRNA. Translation: BAF83480.1.
AC044790 Genomic DNA. Translation: AAK68155.1.
CH471076 Genomic DNA. Translation: EAW74269.1.
BC034384 mRNA. Translation: AAH34384.1.
CCDSCCDS8074.1. [Q9NSA0-1]
RefSeqNP_060954.1. NM_018484.2. [Q9NSA0-1]
UniGeneHs.220844.

3D structure databases

ProteinModelPortalQ9NSA0.
SMRQ9NSA0. Positions 119-529.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120967. 1 interaction.
STRING9606.ENSP00000301891.

Chemistry

ChEMBLCHEMBL2073677.
DrugBankDB01032. Probenecid.

Protein family/group databases

TCDB2.A.1.19.10. the major facilitator superfamily (mfs).

Polymorphism databases

DMDM74734337.

Proteomic databases

PaxDbQ9NSA0.
PRIDEQ9NSA0.

Protocols and materials databases

DNASU55867.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301891; ENSP00000301891; ENSG00000168065. [Q9NSA0-1]
ENST00000377585; ENSP00000366809; ENSG00000168065. [Q9NSA0-2]
GeneID55867.
KEGGhsa:55867.
UCSCuc001oai.3. human. [Q9NSA0-1]
uc009ypq.3. human. [Q9NSA0-2]

Organism-specific databases

CTD55867.
GeneCardsGC11P064324.
HGNCHGNC:18120. SLC22A11.
HPAHPA026076.
MIM607097. gene.
neXtProtNX_Q9NSA0.
PharmGKBPA38295.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000234569.
HOVERGENHBG108433.
InParanoidQ9NSA0.
KOK08207.
OMANPRHPME.
OrthoDBEOG7C5M82.
PhylomeDBQ9NSA0.
TreeFamTF315847.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9NSA0.
BgeeQ9NSA0.
CleanExHS_SLC22A11.
GenevestigatorQ9NSA0.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC22A11. human.
GeneWikiSLC22A11.
GenomeRNAi55867.
NextBio61178.
PROQ9NSA0.
SOURCESearch...

Entry information

Entry nameS22AB_HUMAN
AccessionPrimary (citable) accession number: Q9NSA0
Secondary accession number(s): A8K426 expand/collapse secondary AC list , Q53GR2, Q6ZP72, Q8NBU4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM