Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kinesin-like protein KIF15

Gene

KIF15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi109 – 116ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: ProtInc
  • microtubule motor activity Source: GO_Central
  • motor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163808-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF15
Alternative name(s):
Kinesin-like protein 2
Short name:
hKLP2
Kinesin-like protein 7
Serologically defined breast cancer antigen NY-BR-62
Gene namesi
Name:KIF15
Synonyms:KLP2, KNSL7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17273. KIF15.

Subcellular locationi

  • Cytoplasm
  • Cytoplasmcytoskeletonspindle

  • Note: Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase (By similarity). Localizes at the sites of invaginating cell membranes, a position that corresponds to the location of the contractile actomyosin ring of dividing cells (By similarity). Colocalizes with actin in interphase (By similarity). Colocalizes in dendrites and in growth cone of axons with microtubules (By similarity).By similarity

GO - Cellular componenti

  • centrosome Source: ProtInc
  • cytosol Source: Reactome
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • plus-end kinesin complex Source: ProtInc
  • spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi56992.
OpenTargetsiENSG00000163808.
ENSG00000280610.
PharmGKBiPA30183.

Chemistry databases

ChEMBLiCHEMBL3632454.

Polymorphism and mutation databases

BioMutaiKIF15.
DMDMi74752937.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003286841 – 1388Kinesin-like protein KIF15Add BLAST1388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei399PhosphothreonineCombined sources1
Modified residuei568PhosphoserineCombined sources1
Modified residuei1009N6-acetyllysineCombined sources1
Modified residuei1141PhosphoserineCombined sources1
Modified residuei1169PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NS87.
MaxQBiQ9NS87.
PaxDbiQ9NS87.
PeptideAtlasiQ9NS87.
PRIDEiQ9NS87.

PTM databases

iPTMnetiQ9NS87.
PhosphoSitePlusiQ9NS87.

Expressioni

Tissue specificityi

Expressed in testis, colon, thymus and in breast cancer.1 Publication

Gene expression databases

BgeeiENSG00000163808.
CleanExiHS_KIF15.
ExpressionAtlasiQ9NS87. baseline and differential.
GenevisibleiQ9NS87. HS.

Organism-specific databases

HPAiHPA035516.
HPA035517.

Interactioni

Subunit structurei

Interacts with MKI67 and TPX2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MKI67P460133EBI-712159,EBI-876367

Protein-protein interaction databases

BioGridi121307. 26 interactors.
DIPiDIP-28133N.
IntActiQ9NS87. 18 interactors.
MINTiMINT-138026.
STRINGi9606.ENSP00000324020.

Structurei

Secondary structure

11388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 33Combined sources6
Beta strandi50 – 55Combined sources6
Beta strandi58 – 61Combined sources4
Beta strandi64 – 66Combined sources3
Beta strandi68 – 71Combined sources4
Beta strandi73 – 76Combined sources4
Helixi82 – 98Combined sources17
Beta strandi103 – 108Combined sources6
Helixi115 – 119Combined sources5
Helixi135 – 150Combined sources16
Beta strandi158 – 172Combined sources15
Beta strandi174 – 176Combined sources3
Beta strandi186 – 189Combined sources4
Beta strandi195 – 198Combined sources4
Helixi208 – 226Combined sources19
Beta strandi228 – 231Combined sources4
Beta strandi236 – 250Combined sources15
Beta strandi253 – 265Combined sources13
Helixi290 – 306Combined sources17
Beta strandi307 – 309Combined sources3
Helixi315 – 317Combined sources3
Helixi319 – 323Combined sources5
Helixi325 – 327Combined sources3
Beta strandi330 – 340Combined sources11
Helixi344 – 346Combined sources3
Helixi347 – 360Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BN2X-ray2.70A/B/C19-375[»]
ProteinModelPortaliQ9NS87.
SMRiQ9NS87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 363Kinesin motorPROSITE-ProRule annotationAdd BLAST338

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili368 – 1388Sequence analysisAdd BLAST1021

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KLP2 subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4280. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129680.
HOGENOMiHOG000113225.
HOVERGENiHBG062662.
InParanoidiQ9NS87.
KOiK10400.
OMAiEHHSAQM.
OrthoDBiEOG091G00LM.
PhylomeDBiQ9NS87.
TreeFamiTF320478.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR031794. HMMR_C.
IPR027640. Kinesin-like_fam.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 4 hits.
PfamiPF15908. HMMR_C. 1 hit.
PF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NS87-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPGCKTELR SVTNGQSNQP SNEGDAIKVF VRIRPPAERS GSADGEQNLC
60 70 80 90 100
LSVLSSTSLR LHSNPEPKTF TFDHVADVDT TQESVFATVA KSIVESCMSG
110 120 130 140 150
YNGTIFAYGQ TGSGKTFTMM GPSESDNFSH NLRGVIPRSF EYLFSLIDRE
160 170 180 190 200
KEKAGAGKSF LCKCSFIEIY NEQIYDLLDS ASAGLYLREH IKKGVFVVGA
210 220 230 240 250
VEQVVTSAAE AYQVLSGGWR NRRVASTSMN RESSRSHAVF TITIESMEKS
260 270 280 290 300
NEIVNIRTSL LNLVDLAGSE RQKDTHAEGM RLKEAGNINR SLSCLGQVIT
310 320 330 340 350
ALVDVGNGKQ RHVCYRDSKL TFLLRDSLGG NAKTAIIANV HPGSRCFGET
360 370 380 390 400
LSTLNFAQRA KLIKNKAVVN EDTQGNVSQL QAEVKRLKEQ LAELASGQTP
410 420 430 440 450
PESFLTRDKK KTNYMEYFQE AMLFFKKSEQ EKKSLIEKVT QLEDLTLKKE
460 470 480 490 500
KFIQSNKMIV KFREDQIIRL EKLHKESRGG FLPEEQDRLL SELRNEIQTL
510 520 530 540 550
REQIEHHPRV AKYAMENHSL REENRRLRLL EPVKRAQEMD AQTIAKLEKA
560 570 580 590 600
FSEISGMEKS DKNQQGFSPK AQKEPCLFAN TEKLKAQLLQ IQTELNNSKQ
610 620 630 640 650
EYEEFKELTR KRQLELESEL QSLQKANLNL ENLLEATKAC KRQEVSQLNK
660 670 680 690 700
IHAETLKIIT TPTKAYQLHS RPVPKLSPEM GSFGSLYTQN SSILDNDILN
710 720 730 740 750
EPVPPEMNEQ AFEAISEELR TVQEQMSALQ AKLDEEEHKN LKLQQHVDKL
760 770 780 790 800
EHHSTQMQEL FSSERIDWTK QQEELLSQLN VLEKQLQETQ TKNDFLKSEV
810 820 830 840 850
HDLRVVLHSA DKELSSVKLE YSSFKTNQEK EFNKLSERHM HVQLQLDNLR
860 870 880 890 900
LENEKLLESK ACLQDSYDNL QEIMKFEIDQ LSRNLQNFKK ENETLKSDLN
910 920 930 940 950
NLMELLEAEK ERNNKLSLQF EEDKENSSKE ILKVLEAVRQ EKQKETAKCE
960 970 980 990 1000
QQMAKVQKLE ESLLATEKVI SSLEKSRDSD KKVVADLMNQ IQELRTSVCE
1010 1020 1030 1040 1050
KTETIDTLKQ ELKDINCKYN SALVDREESR VLIKKQEVDI LDLKETLRLR
1060 1070 1080 1090 1100
ILSEDIERDM LCEDLAHATE QLNMLTEASK KHSGLLQSAQ EELTKKEALI
1110 1120 1130 1140 1150
QELQHKLNQK KEEVEQKKNE YNFKMRQLEH VMDSAAEDPQ SPKTPPHFQT
1160 1170 1180 1190 1200
HLAKLLETQE QEIEDGRASK TSLEHLVTKL NEDREVKNAE ILRMKEQLRE
1210 1220 1230 1240 1250
MENLRLESQQ LIEKNWLLQG QLDDIKRQKE NSDQNHPDNQ QLKNEQEESI
1260 1270 1280 1290 1300
KERLAKSKIV EEMLKMKADL EEVQSALYNK EMECLRMTDE VERTQTLESK
1310 1320 1330 1340 1350
AFQEKEQLRS KLEEMYEERE RTSQEMEMLR KQVECLAEEN GKLVGHQNLH
1360 1370 1380
QKIQYVVRLK KENVRLAEET EKLRAENVFL KEKKRSES
Length:1,388
Mass (Da):160,160
Last modified:October 1, 2000 - v1
Checksum:iE127EB4B991CA83A
GO
Isoform 2 (identifier: Q9NS87-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.

Show »
Length:1,291
Mass (Da):149,784
Checksum:i86EABBD49A76151B
GO
Isoform 3 (identifier: Q9NS87-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-952: Missing.

Show »
Length:436
Mass (Da):51,433
Checksum:i3BE3ABBDB7A7821D
GO
Isoform 4 (identifier: Q9NS87-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1092-1119: ELTKKEALIQELQHKLNQKKEEVEQKKN → RTDQERSPDSGTSAQAKPKERGSRTEEE
     1200-1388: Missing.

Show »
Length:1,199
Mass (Da):137,169
Checksum:i18176A5A924EFB95
GO

Sequence cautioni

The sequence AAG48261 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1057E → K in AAG48261 (PubMed:12747765).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042464211A → V.Corresponds to variant rs34862960dbSNPEnsembl.1
Natural variantiVAR_042465996T → S.2 PublicationsCorresponds to variant rs11710339dbSNPEnsembl.1
Natural variantiVAR_0424661206L → M.Corresponds to variant rs3804583dbSNPEnsembl.1
Natural variantiVAR_0424671272E → D.Corresponds to variant rs17076986dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0327521 – 952Missing in isoform 3. 1 PublicationAdd BLAST952
Alternative sequenceiVSP_0327531 – 97Missing in isoform 2. 1 PublicationAdd BLAST97
Alternative sequenceiVSP_0327541092 – 1119ELTKK…EQKKN → RTDQERSPDSGTSAQAKPKE RGSRTEEE in isoform 4. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_0327551200 – 1388Missing in isoform 4. 1 PublicationAdd BLAST189

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035898 mRNA. Translation: BAB03309.1.
AK027816 mRNA. Translation: BAB55389.1.
BC117174 mRNA. Translation: AAI17175.1.
AL832908 mRNA. Translation: CAH10635.1.
AF308294 mRNA. Translation: AAG48261.1. Frameshift.
CCDSiCCDS33744.1. [Q9NS87-1]
RefSeqiNP_064627.1. NM_020242.2. [Q9NS87-1]
UniGeneiHs.658939.

Genome annotation databases

EnsembliENST00000326047; ENSP00000324020; ENSG00000163808. [Q9NS87-1]
ENST00000627272; ENSP00000486004; ENSG00000280610. [Q9NS87-1]
GeneIDi56992.
KEGGihsa:56992.
UCSCiuc003cnx.5. human. [Q9NS87-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035898 mRNA. Translation: BAB03309.1.
AK027816 mRNA. Translation: BAB55389.1.
BC117174 mRNA. Translation: AAI17175.1.
AL832908 mRNA. Translation: CAH10635.1.
AF308294 mRNA. Translation: AAG48261.1. Frameshift.
CCDSiCCDS33744.1. [Q9NS87-1]
RefSeqiNP_064627.1. NM_020242.2. [Q9NS87-1]
UniGeneiHs.658939.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BN2X-ray2.70A/B/C19-375[»]
ProteinModelPortaliQ9NS87.
SMRiQ9NS87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121307. 26 interactors.
DIPiDIP-28133N.
IntActiQ9NS87. 18 interactors.
MINTiMINT-138026.
STRINGi9606.ENSP00000324020.

Chemistry databases

ChEMBLiCHEMBL3632454.

PTM databases

iPTMnetiQ9NS87.
PhosphoSitePlusiQ9NS87.

Polymorphism and mutation databases

BioMutaiKIF15.
DMDMi74752937.

Proteomic databases

EPDiQ9NS87.
MaxQBiQ9NS87.
PaxDbiQ9NS87.
PeptideAtlasiQ9NS87.
PRIDEiQ9NS87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326047; ENSP00000324020; ENSG00000163808. [Q9NS87-1]
ENST00000627272; ENSP00000486004; ENSG00000280610. [Q9NS87-1]
GeneIDi56992.
KEGGihsa:56992.
UCSCiuc003cnx.5. human. [Q9NS87-1]

Organism-specific databases

CTDi56992.
DisGeNETi56992.
GeneCardsiKIF15.
HGNCiHGNC:17273. KIF15.
HPAiHPA035516.
HPA035517.
neXtProtiNX_Q9NS87.
OpenTargetsiENSG00000163808.
ENSG00000280610.
PharmGKBiPA30183.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4280. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129680.
HOGENOMiHOG000113225.
HOVERGENiHBG062662.
InParanoidiQ9NS87.
KOiK10400.
OMAiEHHSAQM.
OrthoDBiEOG091G00LM.
PhylomeDBiQ9NS87.
TreeFamiTF320478.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163808-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.

Miscellaneous databases

GeneWikiiKIF15.
GenomeRNAii56992.
PROiQ9NS87.

Gene expression databases

BgeeiENSG00000163808.
CleanExiHS_KIF15.
ExpressionAtlasiQ9NS87. baseline and differential.
GenevisibleiQ9NS87. HS.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR031794. HMMR_C.
IPR027640. Kinesin-like_fam.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 4 hits.
PfamiPF15908. HMMR_C. 1 hit.
PF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF15_HUMAN
AccessioniPrimary (citable) accession number: Q9NS87
Secondary accession number(s): Q17RV9
, Q69YL6, Q96JX7, Q9H280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.