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Q9NS87 (KIF15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF15
Alternative name(s):
Kinesin-like protein 2
Short name=hKLP2
Kinesin-like protein 7
Serologically defined breast cancer antigen NY-BR-62
Gene names
Name:KIF15
Synonyms:KLP2, KNSL7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1388 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly By similarity.

Subunit structure

Interacts with MKI67 and TPX2. Ref.1 Ref.6

Subcellular location

Cytoplasm. Cytoplasmcytoskeletonspindle. Note: Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase By similarity. Localizes at the sites of invaginating cell membranes, a position that corresponds to the location of the contractile actomyosin ring of dividing cells By similarity. Colocalizes with actin in interphase By similarity. Colocalizes in dendrites and in growth cone of axons with microtubules By similarity. Ref.1 Ref.6

Tissue specificity

Expressed in testis, colon, thymus and in breast cancer. Ref.5

Sequence similarities

Belongs to the kinesin-like protein family. KLP2 subfamily.

Contains 1 kinesin-motor domain.

Sequence caution

The sequence AAG48261.1 differs from that shown. Reason: Frameshift at several positions.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NS87-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NS87-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.
Isoform 3 (identifier: Q9NS87-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-952: Missing.
Isoform 4 (identifier: Q9NS87-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1092-1119: ELTKKEALIQELQHKLNQKKEEVEQKKN → RTDQERSPDSGTSAQAKPKERGSRTEEE
     1200-1388: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13881388Kinesin-like protein KIF15
PRO_0000328684

Regions

Domain23 – 290268Kinesin-motor
Nucleotide binding109 – 1168ATP By similarity
Coiled coil368 – 13881021 Potential

Amino acid modifications

Modified residue5681Phosphoserine Ref.7
Modified residue10091N6-acetyllysine Ref.8
Modified residue11411Phosphoserine Ref.9
Modified residue11441Phosphothreonine Ref.9
Modified residue11691Phosphoserine Ref.9

Natural variations

Alternative sequence1 – 952952Missing in isoform 3.
VSP_032752
Alternative sequence1 – 9797Missing in isoform 2.
VSP_032753
Alternative sequence1092 – 111928ELTKK…EQKKN → RTDQERSPDSGTSAQAKPKE RGSRTEEE in isoform 4.
VSP_032754
Alternative sequence1200 – 1388189Missing in isoform 4.
VSP_032755
Natural variant2111A → V.
Corresponds to variant rs34862960 [ dbSNP | Ensembl ].
VAR_042464
Natural variant9961T → S. Ref.4 Ref.5
Corresponds to variant rs11710339 [ dbSNP | Ensembl ].
VAR_042465
Natural variant12061L → M.
Corresponds to variant rs3804583 [ dbSNP | Ensembl ].
VAR_042466
Natural variant12721E → D.
Corresponds to variant rs17076986 [ dbSNP | Ensembl ].
VAR_042467

Experimental info

Sequence conflict10571E → K in AAG48261. Ref.5

Secondary structure

................................................... 1388
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E127EB4B991CA83A

FASTA1,388160,160
        10         20         30         40         50         60 
MAPGCKTELR SVTNGQSNQP SNEGDAIKVF VRIRPPAERS GSADGEQNLC LSVLSSTSLR 

        70         80         90        100        110        120 
LHSNPEPKTF TFDHVADVDT TQESVFATVA KSIVESCMSG YNGTIFAYGQ TGSGKTFTMM 

       130        140        150        160        170        180 
GPSESDNFSH NLRGVIPRSF EYLFSLIDRE KEKAGAGKSF LCKCSFIEIY NEQIYDLLDS 

       190        200        210        220        230        240 
ASAGLYLREH IKKGVFVVGA VEQVVTSAAE AYQVLSGGWR NRRVASTSMN RESSRSHAVF 

       250        260        270        280        290        300 
TITIESMEKS NEIVNIRTSL LNLVDLAGSE RQKDTHAEGM RLKEAGNINR SLSCLGQVIT 

       310        320        330        340        350        360 
ALVDVGNGKQ RHVCYRDSKL TFLLRDSLGG NAKTAIIANV HPGSRCFGET LSTLNFAQRA 

       370        380        390        400        410        420 
KLIKNKAVVN EDTQGNVSQL QAEVKRLKEQ LAELASGQTP PESFLTRDKK KTNYMEYFQE 

       430        440        450        460        470        480 
AMLFFKKSEQ EKKSLIEKVT QLEDLTLKKE KFIQSNKMIV KFREDQIIRL EKLHKESRGG 

       490        500        510        520        530        540 
FLPEEQDRLL SELRNEIQTL REQIEHHPRV AKYAMENHSL REENRRLRLL EPVKRAQEMD 

       550        560        570        580        590        600 
AQTIAKLEKA FSEISGMEKS DKNQQGFSPK AQKEPCLFAN TEKLKAQLLQ IQTELNNSKQ 

       610        620        630        640        650        660 
EYEEFKELTR KRQLELESEL QSLQKANLNL ENLLEATKAC KRQEVSQLNK IHAETLKIIT 

       670        680        690        700        710        720 
TPTKAYQLHS RPVPKLSPEM GSFGSLYTQN SSILDNDILN EPVPPEMNEQ AFEAISEELR 

       730        740        750        760        770        780 
TVQEQMSALQ AKLDEEEHKN LKLQQHVDKL EHHSTQMQEL FSSERIDWTK QQEELLSQLN 

       790        800        810        820        830        840 
VLEKQLQETQ TKNDFLKSEV HDLRVVLHSA DKELSSVKLE YSSFKTNQEK EFNKLSERHM 

       850        860        870        880        890        900 
HVQLQLDNLR LENEKLLESK ACLQDSYDNL QEIMKFEIDQ LSRNLQNFKK ENETLKSDLN 

       910        920        930        940        950        960 
NLMELLEAEK ERNNKLSLQF EEDKENSSKE ILKVLEAVRQ EKQKETAKCE QQMAKVQKLE 

       970        980        990       1000       1010       1020 
ESLLATEKVI SSLEKSRDSD KKVVADLMNQ IQELRTSVCE KTETIDTLKQ ELKDINCKYN 

      1030       1040       1050       1060       1070       1080 
SALVDREESR VLIKKQEVDI LDLKETLRLR ILSEDIERDM LCEDLAHATE QLNMLTEASK 

      1090       1100       1110       1120       1130       1140 
KHSGLLQSAQ EELTKKEALI QELQHKLNQK KEEVEQKKNE YNFKMRQLEH VMDSAAEDPQ 

      1150       1160       1170       1180       1190       1200 
SPKTPPHFQT HLAKLLETQE QEIEDGRASK TSLEHLVTKL NEDREVKNAE ILRMKEQLRE 

      1210       1220       1230       1240       1250       1260 
MENLRLESQQ LIEKNWLLQG QLDDIKRQKE NSDQNHPDNQ QLKNEQEESI KERLAKSKIV 

      1270       1280       1290       1300       1310       1320 
EEMLKMKADL EEVQSALYNK EMECLRMTDE VERTQTLESK AFQEKEQLRS KLEEMYEERE 

      1330       1340       1350       1360       1370       1380 
RTSQEMEMLR KQVECLAEEN GKLVGHQNLH QKIQYVVRLK KENVRLAEET EKLRAENVFL 


KEKKRSES 

« Hide

Isoform 2 [UniParc].

Checksum: 86EABBD49A76151B
Show »

FASTA1,291149,784
Isoform 3 [UniParc].

Checksum: 3BE3ABBDB7A7821D
Show »

FASTA43651,433
Isoform 4 [UniParc].

Checksum: 18176A5A924EFB95
Show »

FASTA1,199137,169

References

« Hide 'large scale' references
[1]"The forkhead-associated domain of Ki-67 antigen interacts with the novel kinesin-like protein Hklp2."
Sueishi M., Takagi M., Yoneda Y.
J. Biol. Chem. 275:28888-28892(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MKI67, SUBCELLULAR LOCATION.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Placenta.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Cerebellum.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 339-1388 (ISOFORMS 1/2), VARIANT SER-996.
Tissue: Melanoma.
[5]"Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression."
Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.
Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 786-1388 (ISOFORM 4), TISSUE SPECIFICITY, VARIANT SER-996.
Tissue: Mammary gland.
[6]"repp86: a human protein associated in the progression of mitosis."
Heidebrecht H.-J., Adam-Klages S., Szczepanowski M., Pollmann M., Buck F., Endl E., Kruse M.-L., Rudolph P., Parwaresch R.
Mol. Cancer Res. 1:271-279(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TPX2, SUBCELLULAR LOCATION.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1009, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1141; THR-1144 AND SER-1169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB035898 mRNA. Translation: BAB03309.1.
AK027816 mRNA. Translation: BAB55389.1.
BC117174 mRNA. Translation: AAI17175.1.
AL832908 mRNA. Translation: CAH10635.1.
AF308294 mRNA. Translation: AAG48261.1. Frameshift.
RefSeqNP_064627.1. NM_020242.2.
XP_005265385.1. XM_005265328.1.
UniGeneHs.658939.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BN2X-ray2.70A/B/C19-375[»]
ProteinModelPortalQ9NS87.
SMRQ9NS87. Positions 21-389.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121307. 11 interactions.
DIPDIP-28133N.
IntActQ9NS87. 4 interactions.
MINTMINT-138026.
STRING9606.ENSP00000324020.

PTM databases

PhosphoSiteQ9NS87.

Polymorphism databases

DMDM74752937.

Proteomic databases

PaxDbQ9NS87.
PRIDEQ9NS87.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000326047; ENSP00000324020; ENSG00000163808. [Q9NS87-1]
GeneID56992.
KEGGhsa:56992.
UCSCuc003cnx.4. human. [Q9NS87-1]
uc010hir.3. human. [Q9NS87-3]

Organism-specific databases

CTD56992.
GeneCardsGC03P044803.
HGNCHGNC:17273. KIF15.
HPAHPA035516.
HPA035517.
neXtProtNX_Q9NS87.
PharmGKBPA30183.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5059.
HOGENOMHOG000113225.
HOVERGENHBG062662.
InParanoidQ9NS87.
KOK10400.
OMAEHHSAQM.
OrthoDBEOG7M0NQQ.
PhylomeDBQ9NS87.
TreeFamTF320478.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressQ9NS87.
BgeeQ9NS87.
CleanExHS_KIF15.
GenevestigatorQ9NS87.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR027640. Kinesin-like_fam.
IPR024658. Kinesin-like_KLP2.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 1 hit.
PF12711. Kinesin-relat_1. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKIF15.
GenomeRNAi56992.
NextBio62689.
PROQ9NS87.

Entry information

Entry nameKIF15_HUMAN
AccessionPrimary (citable) accession number: Q9NS87
Secondary accession number(s): Q17RV9 expand/collapse secondary AC list , Q69YL6, Q96JX7, Q9H280
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM