Q9NS86 (LANC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LanC-like protein 2

Alternative name(s):
Testis-specific adriamycin sensitivity protein

Gene names

Name:	LANCL2
Synonyms:	GPR69B, TASP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes. Ref.11 Ref.12
Subunit structure	Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). PIP-binding enhances membrane association. Ref.10
Subcellular location	Nucleus. Cytoplasm. Cell membrane. Note: Localizes to the juxta-nuclear vesicles. Associates with the cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-cyclodextrin causes partial dissociation from the cell membrane in vitro and an enhanced cell detachment from the matrix in vivo. Membrane-association is important for the increased cellular sensitivity to an anticancer drug (adriamycin). Ref.10 Ref.11
Tissue specificity	Expressed in brain and testis. Ref.1
Post-translational modification	Myristoylated. Essential for membrane association.
Miscellaneous	Its exogenous expression in a sarcoma cell line decreases the expression of ABCB1 (P-glycoprotein 1) and increases cellular sensitivity to an anticancer drug (adriamycin).
Sequence similarities	Belongs to the LanC-like protein family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Coding sequence diversity	Polymorphism
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.11. Source: UniProtKB positive regulation of abscisic acid mediated signaling pathway Inferred from direct assay Ref.12. Source: UniProtKB
Cellular component	cortical actin cytoskeleton Inferred from direct assay Ref.10. Source: UniProtKB cytosol Inferred from direct assay Ref.11. Source: UniProtKB nucleus Inferred from direct assay Ref.11. Source: UniProtKB plasma membrane Inferred from direct assay Ref.11Ref.10. Source: UniProtKB
Molecular function	ATP binding Non-traceable author statement Ref.1. Source: UniProtKB GTP binding Non-traceable author statement Ref.1. Source: UniProtKB catalytic activity Inferred from electronic annotation. Source: InterPro phosphatidylinositol-3-phosphate binding Inferred from direct assay Ref.10. Source: UniProtKB phosphatidylinositol-4-phosphate binding Inferred from direct assay Ref.10. Source: UniProtKB phosphatidylinositol-5-phosphate binding Inferred from direct assay Ref.10. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 450	449	LanC-like protein 2		PRO_0000191272
Regions
	Region	2 – 15	14	Interaction with inositol phospholipids
Amino acid modifications
	Modified residue	198	1	Phosphotyrosine By similarity
	Modified residue	259	1	Phosphotyrosine By similarity
	Modified residue	295	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine Ref.10
Natural variations
	Natural variant	56	1	T → P. Ref.6 Ref.7 Ref.8 Corresponds to variant rs2272263 [ dbSNP | Ensembl ].		VAR_060064
	Natural variant	74	1	I → V. Corresponds to variant rs6961412 [ dbSNP | Ensembl ].		VAR_053480
Experimental info
	Mutagenesis	2	1	G → A: Loss of membrane localization and results in localization to the nucleus, particularly to the nucleoli. Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q9NS86 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8FD27E11BB91C05D	FASTA	450	50,854
	10 20 30 40 50 60 MGETMSKRLK LHLGGEAEME ERAFVNPFPD YEAAAGALLA SGAAEETGCV RPPATTDEPG 70 80 90 100 110 120 LPFHQDGKII HNFIRRIQTK IKDLLQQMEE GLKTADPHDC SAYTGWTGIA LLYLQLYRVT 130 140 150 160 170 180 CDQTYLLRSL DYVKRTLRNL NGRRVTFLCG DAGPLAVGAV IYHKLRSDCE SQECVTKLLQ 190 200 210 220 230 240 LQRSVVCQES DLPDELLYGR AGYLYALLYL NTEIGPGTVC ESAIKEVVNA IIESGKTLSR 250 260 270 280 290 300 EERKTERCPL LYQWHRKQYV GAAHGMAGIY YMLMQPAAKV DQETLTEMVK PSIDYVRHKK 310 320 330 340 350 360 FRSGNYPSSL SNETDRLVHW CHGAPGVIHM LMQAYKVFKE EKYLKEAMEC SDVIWQRGLL 370 380 390 400 410 420 RKGYGICHGT AGNGYSFLSL YRLTQDKKYL YRACKFAEWC LDYGAHGCRI PDRPYSLFEG 430 440 450 MAGAIHFLSD VLGPETSRFP AFELDSSKRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, characterization, and tissue-specific expression of human LANCL2, a novel member of the LanC-like protein family." Mayer H., Pongratz M., Prohaska R. DNA Seq. 12:161-166(2001) [PubMed: 11762191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Isolation and identification of genes whose downregulation result in anticancer drug resistance." Sugimoto Y., Tsukahara S., Ishikawa E., Tsuruo T. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"2 MB physical and transcript map of the 7p11.2 locus surrounding EGFR frequently amplified in glioblastoma multiforme." Eley G.D., Wang X.-Y., Frederick L., Hebrink D., Nash C., James C.D. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-56.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-56.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-56. Tissue: Ovary.
[9]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-450. Tissue: Testis.
[10]	"Myristoylation of human LanC-like protein 2 (LANCL2) is essential for the interaction with the plasma membrane and the increase in cellular sensitivity to adriamycin." Landlinger C., Salzer U., Prohaska R. Biochim. Biophys. Acta 1758:1759-1767(2006) [PubMed: 16979580] [Abstract] Cited for: SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2, INTERACTION WITH INOSITOL PHOSPHOLIPIDS.
[11]	"Lanthionine synthetase components C-like 2 increases cellular sensitivity to adriamycin by decreasing the expression of P-glycoprotein through a transcription-mediated mechanism." Park S., James C.D. Cancer Res. 63:723-727(2003) [PubMed: 12566319] [Abstract] Cited for: SUBCELLULAR LOCATION, POSSIBLE FUNCTION IN CELLULAR SENSITIVITY TO ADRIAMYCIN.
[12]	"LANCL2 is necessary for abscisic acid binding and signaling in human granulocytes and in rat insulinoma cells." Sturla L., Fresia C., Guida L., Bruzzone S., Scarfi' S., Usai C., Fruscione F., Magnone M., Millo E., Basile G., Grozio A., Jacchetti E., Allegretti M., De Flora A., Zocchi E. J. Biol. Chem. 284:28045-28057(2009) [PubMed: 19667068] [Abstract] Cited for: FUNCTION.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278245 mRNA. Translation: CAC21715.1. AB035966 mRNA. Translation: BAA96800.1. AF353942 mRNA. Translation: AAK83286.1. AK313326 mRNA. Translation: BAG36131.1. AC073347 Genomic DNA. Translation: AAQ93360.1. CH236957 Genomic DNA. Translation: EAL23810.1. CH471201 Genomic DNA. Translation: EAW50968.1. BC004887 mRNA. Translation: AAH04887.2. BC070049 mRNA. Translation: AAH70049.1. AL713665 mRNA. Translation: CAD28471.1.
IPI	IPI00032995.
RefSeq	NP_061167.1. NM_018697.3.
UniGene	Hs.595384.
3D structure databases
ProteinModelPortal	Q9NS86.
SMR	Q9NS86. Positions 19-444.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NS86. 1 interaction.
STRING	Q9NS86.
PTM databases
PhosphoSite	Q9NS86.
Polymorphism databases
DMDM	47116933.
Proteomic databases
PRIDE	Q9NS86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000254770; ENSP00000254770; ENSG00000132434.
GeneID	55915.
KEGG	hsa:55915.
UCSC	uc003tqp.1. human.
Organism-specific databases
CTD	55915.
GeneCards	GC07P055400.
H-InvDB	HIX0006697.
HGNC	HGNC:6509. LANCL2.
HPA	HPA019711. HPA029535.
MIM	612919. gene.
neXtProt	NX_Q9NS86.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08224.
GeneTree	ENSGT00530000063186.
HOGENOM	HBG715012.
HOVERGEN	HBG065387.
InParanoid	Q9NS86.
OMA	EAMECSD.
OrthoDB	EOG4CZBG8.
PhylomeDB	Q9NS86.
Gene expression databases
ArrayExpress	Q9NS86.
Bgee	Q9NS86.
CleanEx	HS_LANCL2.
Genevestigator	Q9NS86.
GermOnline	ENSG00000132434. Homo sapiens.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR007822. LANC-like. IPR020464. LanC-like_prot_euk. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
Pfam	PF05147. LANC_like. 1 hit. [Graphical view]
PRINTS	PR01951. LANCEUKARYTE. PR01950. LANCSUPER.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...
Other
NextBio	61311.
SOURCE	Search...

Entry information

Entry name

LANC2_HUMAN

Accession

Primary (citable) accession number: Q9NS86
Secondary accession number(s): B2R8D4 , Q6NSL4, Q8TCQ3, Q9BSR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.