Q9NS75 (CLTR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteinyl leukotriene receptor 2
Short name=CysLTR2

Alternative name(s):
G-protein coupled receptor GPCR21
Short name=hGPCR21
G-protein coupled receptor HG57
HPN321

Gene names

Name:	CYSLTR2
Synonyms:	CYSLT2, CYSLT2R
ORF Names:	PSEC0146

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4 >> LTE4.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed, with highest levels in the heart, placenta, spleen, peripheral blood leukocytes and adrenal gland. In lung, expressed in the interstitial macrophages, and slightly in smooth muscle cells.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	immune response Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of cell death Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	cysteinyl leukotriene receptor activity Inferred from electronic annotation. Source: Compara leukotriene receptor activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Cysteinyl leukotriene receptor 2		PRO_0000069303
Regions
	Topological domain	1 – 42	42	Extracellular Potential
	Transmembrane	43 – 63	21	Helical; Name=1; Potential
	Topological domain	64 – 72	9	Cytoplasmic Potential
	Transmembrane	73 – 93	21	Helical; Name=2; Potential
	Topological domain	94 – 123	30	Extracellular Potential
	Transmembrane	124 – 144	21	Helical; Name=3; Potential
	Topological domain	145 – 153	9	Cytoplasmic Potential
	Transmembrane	154 – 174	21	Helical; Name=4; Potential
	Topological domain	175 – 204	30	Extracellular Potential
	Transmembrane	205 – 225	21	Helical; Name=5; Potential
	Topological domain	226 – 245	20	Cytoplasmic Potential
	Transmembrane	246 – 266	21	Helical; Name=6; Potential
	Topological domain	267 – 286	20	Extracellular Potential
	Transmembrane	287 – 307	21	Helical; Name=7; Potential
	Topological domain	308 – 346	39	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	20	1	N-linked (GlcNAc...) Potential
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Disulfide bond	111 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9NS75 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: EB54A4A2DDCE5EE4	FASTA	346	39,635
	10 20 30 40 50 60 MERKFMSLQP SISVSEMEPN GTFSNNNSRN CTIENFKREF FPIVYLIIFF WGVLGNGLSI 70 80 90 100 110 120 YVFLQPYKKS TSVNVFMLNL AISDLLFIST LPFRADYYLR GSNWIFGDLA CRIMSYSLYV 130 140 150 160 170 180 NMYSSIYFLT VLSVVRFLAM VHPFRLLHVT SIRSAWILCG IIWILIMASS IMLLDSGSEQ 190 200 210 220 230 240 NGSVTSCLEL NLYKIAKLQT MNYIALVVGC LLPFFTLSIC YLLIIRVLLK VEVPESGLRV 250 260 270 280 290 300 SHRKALTTII ITLIIFFLCF LPYHTLRTVH LTTWKVGLCK DRLHKALVIT LALAAANACF 310 320 330 340 NPLLYYFAGE NFKDRLKSAL RKGHPQKAKT KCVFPVSVWL RKETRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The molecular characterization and tissue distribution of the human cysteinyl leukotriene CysLT2 receptor." Takasaki J., Kamohara M., Matsumoto M., Saito T., Sugimoto T., Ota T., Nishikawa T., Kawai Y., Masuho Y., Isogai T., Suzuki Y., Sugano S., Furuichi K. Biochem. Biophys. Res. Commun. 274:316-322(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Characterization of the human cysteinyl leukotriene 2 receptor." Heise C.E., O'Dowd B.F., Figueroa D.J., Sawyer N., Nguyen T., Im D.-S., Stocco R., Bellefeuille J.N., Abramovitz M., Cheng R., Williams D.L. Jr., Zeng Z., Liu Q., Ma L., Clements M.K., Coulombe N., Liu Y., Austin C.P. , George S.R., O'Neill G.P., Metters K.M., Lynch K.R., Evans J.F. J. Biol. Chem. 275:30531-30536(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular cloning and characterization of a second human cysteinyl leukotriene receptor: discovery of a subtype selective agonist." Nothacker H.-P., Wang Z., Zhu Y., Reinscheid R.K., Lin S.H.S., Civelli O. Mol. Pharmacol. 58:1601-1608(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Identification of G protein-coupled receptor genes from the human genome sequence." Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S. FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Homo sapiens cysteinyl leukotriene receptor 1 like receptor." Suga H. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-346.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038269 mRNA. Translation: BAB03601.1. AF254664 Genomic DNA. Translation: AAG17281.1. AF279611 Genomic DNA. Translation: AAK69485.1. AB083603 Genomic DNA. Translation: BAB89316.1. AY389504 Genomic DNA. Translation: AAQ91330.1. AL137118 Genomic DNA. Translation: CAC29102.1. BC069160 mRNA. Translation: AAH69160.1. AB041644 Genomic DNA. Translation: BAB16379.1.
IPI	IPI00006914.
RefSeq	NP_065110.1. NM_020377.2.
UniGene	Hs.253706. Hs.614318.
3D structure databases
ProteinModelPortal	Q9NS75.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NS75. 1 interaction.
STRING	9606.ENSP00000282018.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	20138034.
Proteomic databases
PaxDb	Q9NS75.
PRIDE	Q9NS75.
Protocols and materials databases
DNASU	57105.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282018; ENSP00000282018; ENSG00000152207.
GeneID	57105.
KEGG	hsa:57105.
UCSC	uc001vck.2. human.
Organism-specific databases
CTD	57105.
GeneCards	GC13P049280.
HGNC	HGNC:18274. CYSLTR2.
HPA	HPA046528.
MIM	605666. gene.
neXtProt	NX_Q9NS75.
PharmGKB	PA38518.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG119185.
HOGENOM	HOG000236328.
HOVERGEN	HBG099611.
InParanoid	Q9NS75.
KO	K04323.
OMA	HPFRLLH.
OrthoDB	EOG4PNXH8.
PhylomeDB	Q9NS75.
Enzyme and pathway databases
Pathway_Interaction_DB	endothelinpathway. Endothelins.
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9NS75.
Bgee	Q9NS75.
CleanEx	HS_CYSLTR2.
Genevestigator	Q9NS75.
GermOnline	ENSG00000152207. Homo sapiens.
Family and domain databases
InterPro	IPR013311. CLT2_recept. IPR004071. Cyst_leuk_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01903. CYSLT2RECPTR. PR01533. CYSLTRECPTR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9NS75.
ChEMBL	CHEMBL4330.
DrugBank	DB00716. Nedocromil.
GenomeRNAi	57105.
NextBio	62945.
SOURCE	Search...

Entry information

Entry name

CLTR2_HUMAN

Accession

Primary (citable) accession number: Q9NS75
Secondary accession number(s): Q9HCQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.