Q9NS71 (GKN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrokine-1

Alternative name(s):
18 kDa antrum mucosa protein
Short name=AMP-18
Protein CA11

Gene names

Name:	GKN1
Synonyms:	AMP18, CA11
ORF Names:	UNQ489/PRO1005

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. Ref.2
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in stomach. No expression is detected in cancer tissue or gastric cancer cell lines. Ref.1 Ref.2
Sequence similarities	Belongs to the gastrokine family. Contains 1 BRICHOS domain.
Caution	It is uncertain whether Met-1 or Met-15 is the initiator.
Sequence caution	The sequence AAN75447.1 differs from that shown. Reason: Erroneous initiation. The sequence AAN75449.1 differs from that shown. Reason: Erroneous initiation. The sequence AAQ89409.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Mitogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	digestion Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell secretory granule Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	By similarity
	Chain	35 – 199	165	Gastrokine-1		PRO_0000021332
Regions
	Domain	68 – 164	97	BRICHOS
Natural variations
	Natural variant	118	1	K → T in a breast cancer sample; somatic mutation. Ref.6		VAR_035923

Sequences

	Sequence		Length	Mass (Da)
	Q9NS71 [UniParc]. Last modified February 10, 2009. Version 3. Checksum: C099B8B9A1338D7A	FASTA	199	21,999
	10 20 30 40 50 60 MLAYSSVHCF REDKMKFTIV FAGLLGVFLA PALANYNINV NDDNNNAGSG QQSVSVNNEH 70 80 90 100 110 120 NVANVDNNNG WDSWNSIWDY GNGFAATRLF QKKTCIVHKM NKEVMPSIQS LDALVKEKKL 130 140 150 160 170 180 QGKGPGGPPP KGLMYSVNPN KVDDLSKFGK NIANMCRGIP TYMAEEMQEA SLFFYSGTCY 190 TTSVLWIVDI SFCGDTVEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of two novel genes, down-regulated in gastric cancer." Yoshikawa Y., Mukai H., Hino F., Asada K., Kato I. Jpn. J. Cancer Res. 91:459-463(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Stomach.
[2]	"A novel mitogenic protein that is highly expressed in cells of the gastric antrum mucosa." Martin T.E., Powell C.T., Wang Z., Bhattacharyya S., Walsh-Reitz M.M., Agarwal K., Toback F.G. Am. J. Physiol. 285:G332-G343(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Stomach.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-199. Tissue: Colon.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-199.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-118.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB039886 mRNA. Translation: BAA92433.1. AY139182 Genomic DNA. Translation: AAN75447.1. Different initiation. AY139184 mRNA. Translation: AAN75449.1. Different initiation. AC114802 Genomic DNA. No translation available. BC059778 mRNA. Translation: AAH59778.2. AY359050 mRNA. Translation: AAQ89409.1. Different initiation.
IPI	IPI00749381.
RefSeq	NP_062563.3. NM_019617.3.
UniGene	Hs.69319.
3D structure databases
ProteinModelPortal	Q9NS71.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NS71. 1 interaction.
PTM databases
PhosphoSite	Q9NS71.
Polymorphism databases
DMDM	223590223.
Proteomic databases
PaxDb	Q9NS71.
PRIDE	Q9NS71.
Protocols and materials databases
DNASU	56287.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000377938; ENSP00000367172; ENSG00000169605.
GeneID	56287.
KEGG	hsa:56287.
UCSC	uc002sfc.3. human.
Organism-specific databases
CTD	56287.
GeneCards	GC02P069114.
HGNC	HGNC:23217. GKN1.
HPA	HPA047684.
MIM	606402. gene.
neXtProt	NX_Q9NS71.
PharmGKB	PA134898851.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG79770.
HOGENOM	HOG000112728.
HOVERGEN	HBG009322.
InParanoid	Q9NS71.
OMA	PTYMAEE.
OrthoDB	EOG4F4SC9.
Gene expression databases
ArrayExpress	Q9NS71.
Bgee	Q9NS71.
CleanEx	HS_CA11. HS_GKN1.
Genevestigator	Q9NS71.
GermOnline	ENSG00000169605. Homo sapiens.
Family and domain databases
InterPro	IPR007084. BRICHOS_dom. [Graphical view]
Pfam	PF04089. BRICHOS. 1 hit. [Graphical view]
SMART	SM01039. BRICHOS. 1 hit. [Graphical view]
PROSITE	PS50869. BRICHOS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	56287.
NextBio	61955.
SOURCE	Search...

Entry information

Entry name

GKN1_HUMAN

Accession

Primary (citable) accession number: Q9NS71
Secondary accession number(s): Q8IUA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	February 10, 2009
Last modified:	April 3, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.