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Protein

Double-stranded RNA-specific editase B2

Gene

ADARB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lacks editing activity. It prevents the binding of other ADAR enzymes to targets in vitro, and decreases the efficiency of these enzymes. Capable of binding to dsRNA but also to ssRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi432 – 4321ZincPROSITE-ProRule annotation
Active sitei434 – 4341Proton donorPROSITE-ProRule annotation
Metal bindingi490 – 4901ZincPROSITE-ProRule annotation
Metal bindingi555 – 5551ZincPROSITE-ProRule annotation

GO - Molecular functioni

  1. adenosine deaminase activity Source: ProtInc
  2. double-stranded RNA binding Source: ProtInc
  3. metal ion binding Source: UniProtKB-KW
  4. poly(A) RNA binding Source: UniProtKB
  5. single-stranded RNA binding Source: ProtInc

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Double-stranded RNA-specific editase B2 (EC:3.5.-.-)
Alternative name(s):
RNA-dependent adenosine deaminase 3
RNA-editing deaminase 2
RNA-editing enzyme 2
dsRNA adenosine deaminase B2
Gene namesi
Name:ADARB2
Synonyms:ADAR3, RED2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:227. ADARB2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24557.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 739739Double-stranded RNA-specific editase B2PRO_0000171782Add
BLAST

Proteomic databases

PaxDbiQ9NS39.
PRIDEiQ9NS39.

2D gel databases

SWISS-2DPAGEQ9NS39.

PTM databases

PhosphoSiteiQ9NS39.

Expressioni

Tissue specificityi

Brain specific. Expressed at higher levels in astrocytomas as compared to the normal brain tissue.2 Publications

Gene expression databases

BgeeiQ9NS39.
CleanExiHS_ADARB2.
ExpressionAtlasiQ9NS39. baseline and differential.
GenevestigatoriQ9NS39.

Organism-specific databases

HPAiHPA031332.
HPA031333.

Interactioni

Protein-protein interaction databases

BioGridi106619. 7 interactions.
IntActiQ9NS39. 1 interaction.
MINTiMINT-4728108.
STRINGi9606.ENSP00000370713.

Structurei

3D structure databases

ProteinModelPortaliQ9NS39.
SMRiQ9NS39. Positions 124-341, 349-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 19167DRBM 1PROSITE-ProRule annotationAdd
BLAST
Domaini274 – 34168DRBM 2PROSITE-ProRule annotationAdd
BLAST
Domaini408 – 735328A to I editasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 3513R-domain (ssRNA-binding)Add
BLAST

Sequence similaritiesi

Contains 1 A to I editase domain.PROSITE-ProRule annotation
Contains 2 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG292433.
GeneTreeiENSGT00550000074412.
HOGENOMiHOG000290164.
HOVERGENiHBG003836.
InParanoidiQ9NS39.
KOiK13194.
OMAiCKLQLIW.
OrthoDBiEOG7VHSX4.
PhylomeDBiQ9NS39.
TreeFamiTF315806.

Family and domain databases

Gene3Di3.30.160.20. 2 hits.
InterProiIPR002466. A_deamin.
IPR014720. dsRNA-bd_dom.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
PF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
SM00358. DSRM. 2 hits.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
PS50137. DS_RBD. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NS39-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVLGSGRG SGGLSSQLKC KSKRRRRRRS KRKDKVSILS TFLAPFKHLS
60 70 80 90 100
PGITNTEDDD TLSTSSAEVK ENRNVGNLAA RPPPSGDRAR GGAPGAKRKR
110 120 130 140 150
PLEEGNGGHL CKLQLVWKKL SWSVAPKNAL VQLHELRPGL QYRTVSQTGP
160 170 180 190 200
VHAPVFAVAV EVNGLTFEGT GPTKKKAKMR AAELALRSFV QFPNACQAHL
210 220 230 240 250
AMGGGPGPGT DFTSDQADFP DTLFQEFEPP APRPGLAGGR PGDAALLSAA
260 270 280 290 300
YGRRRLLCRA LDLVGPTPAT PAAPGERNPV VLLNRLRAGL RYVCLAEPAE
310 320 330 340 350
RRARSFVMAV SVDGRTFEGS GRSKKLARGQ AAQAALQELF DIQMPGHAPG
360 370 380 390 400
RARRTPMPQE FADSISQLVT QKFREVTTDL TPMHARHKAL AGIVMTKGLD
410 420 430 440 450
ARQAQVVALS SGTKCISGEH LSDQGLVVND CHAEVVARRA FLHFLYTQLE
460 470 480 490 500
LHLSKRREDS ERSIFVRLKE GGYRLRENIL FHLYVSTSPC GDARLHSPYE
510 520 530 540 550
ITTDLHSSKH LVRKFRGHLR TKIESGEGTV PVRGPSAVQT WDGVLLGEQL
560 570 580 590 600
ITMSCTDKIA RWNVLGLQGA LLSHFVEPVY LQSIVVGSLH HTGHLARVMS
610 620 630 640 650
HRMEGVGQLP ASYRHNRPLL SGVSDAEARQ PGKSPPFSMN WVVGSADLEI
660 670 680 690 700
INATTGRRSC GGPSRLCKHV LSARWARLYG RLSTRTPSPG DTPSMYCEAK
710 720 730
LGAHTYQSVK QQLFKAFQKA GLGTWVRKPP EQQQFLLTL
Length:739
Mass (Da):80,621
Last modified:October 1, 2000 - v1
Checksum:iA158A81D35894F79
GO
Isoform 2 (identifier: Q9NS39-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MASVLGSGRG...DTLSTSSAEV → MGLWSPVPGN...GAPLTAGSPH
     70-560: Missing.

Note: No experimental confirmation available.

Show »
Length:248
Mass (Da):26,723
Checksum:i370AD0A469831419
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti44 – 441A → T.
Corresponds to variant rs3793733 [ dbSNP | Ensembl ].
VAR_020438
Natural varianti210 – 2101T → M in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_035806
Natural varianti512 – 5121V → I in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_035807
Natural varianti626 – 6261A → T.1 Publication
Corresponds to variant rs2271275 [ dbSNP | Ensembl ].
VAR_048726

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6969MASVL…SSAEV → MGLWSPVPGNSVPRAPVMAG LGLGHSQGAPQGPHDGKTDR SSGPGRRRTERTRLPGLLGG APLTAGSPH in isoform 2. 1 PublicationVSP_056926Add
BLAST
Alternative sequencei70 – 560491Missing in isoform 2. 1 PublicationVSP_056927Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034837 mRNA. Translation: AAF78094.1.
AK055107 mRNA. Translation: BAG51468.1.
AL442069 Genomic DNA. No translation available.
AL450386 Genomic DNA. No translation available.
AL513304, AC026224, AL392083 Genomic DNA. Translation: CAH73405.1.
AL392083, AC026224, AL513304 Genomic DNA. Translation: CAH72171.1.
BC137477 mRNA. Translation: AAI37478.1.
BC140852 mRNA. Translation: AAI40853.1.
CCDSiCCDS7058.1. [Q9NS39-1]
RefSeqiNP_061172.1. NM_018702.3. [Q9NS39-1]
UniGeneiHs.586663.

Genome annotation databases

EnsembliENST00000381310; ENSP00000370711; ENSG00000185736. [Q9NS39-2]
ENST00000381312; ENSP00000370713; ENSG00000185736. [Q9NS39-1]
GeneIDi105.
KEGGihsa:105.
UCSCiuc001igm.4. human.
uc009xhq.3. human. [Q9NS39-1]

Polymorphism databases

DMDMi33112436.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034837 mRNA. Translation: AAF78094.1.
AK055107 mRNA. Translation: BAG51468.1.
AL442069 Genomic DNA. No translation available.
AL450386 Genomic DNA. No translation available.
AL513304, AC026224, AL392083 Genomic DNA. Translation: CAH73405.1.
AL392083, AC026224, AL513304 Genomic DNA. Translation: CAH72171.1.
BC137477 mRNA. Translation: AAI37478.1.
BC140852 mRNA. Translation: AAI40853.1.
CCDSiCCDS7058.1. [Q9NS39-1]
RefSeqiNP_061172.1. NM_018702.3. [Q9NS39-1]
UniGeneiHs.586663.

3D structure databases

ProteinModelPortaliQ9NS39.
SMRiQ9NS39. Positions 124-341, 349-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106619. 7 interactions.
IntActiQ9NS39. 1 interaction.
MINTiMINT-4728108.
STRINGi9606.ENSP00000370713.

PTM databases

PhosphoSiteiQ9NS39.

Polymorphism databases

DMDMi33112436.

2D gel databases

SWISS-2DPAGEQ9NS39.

Proteomic databases

PaxDbiQ9NS39.
PRIDEiQ9NS39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000381310; ENSP00000370711; ENSG00000185736. [Q9NS39-2]
ENST00000381312; ENSP00000370713; ENSG00000185736. [Q9NS39-1]
GeneIDi105.
KEGGihsa:105.
UCSCiuc001igm.4. human.
uc009xhq.3. human. [Q9NS39-1]

Organism-specific databases

CTDi105.
GeneCardsiGC10M001219.
HGNCiHGNC:227. ADARB2.
HPAiHPA031332.
HPA031333.
MIMi602065. gene.
neXtProtiNX_Q9NS39.
PharmGKBiPA24557.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG292433.
GeneTreeiENSGT00550000074412.
HOGENOMiHOG000290164.
HOVERGENiHBG003836.
InParanoidiQ9NS39.
KOiK13194.
OMAiCKLQLIW.
OrthoDBiEOG7VHSX4.
PhylomeDBiQ9NS39.
TreeFamiTF315806.

Miscellaneous databases

ChiTaRSiADARB2. human.
GeneWikiiADARB2.
GenomeRNAii105.
NextBioi409.
PROiQ9NS39.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NS39.
CleanExiHS_ADARB2.
ExpressionAtlasiQ9NS39. baseline and differential.
GenevestigatoriQ9NS39.

Family and domain databases

Gene3Di3.30.160.20. 2 hits.
InterProiIPR002466. A_deamin.
IPR014720. dsRNA-bd_dom.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
PF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
SM00358. DSRM. 2 hits.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
PS50137. DS_RBD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A third member of the RNA-specific adenosine deaminase gene family, ADAR3, contains both single- and double-stranded RNA binding domains."
    Chen C.-X., Cho D.S., Wang Q., Lai F., Carter K.C., Nishikura K.
    RNA 6:755-767(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, REGION R-DOMAIN, RNA-BINDING, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-626.
  5. "Down-regulation of RNA editing in pediatric astrocytomas: ADAR2 editing activity inhibits cell migration and proliferation."
    Cenci C., Barzotti R., Galeano F., Corbelli S., Rota R., Massimi L., Di Rocco C., O'Connell M.A., Gallo A.
    J. Biol. Chem. 283:7251-7260(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Activity regulation of adenosine deaminases acting on RNA (ADARs)."
    Orlandi C., Barbon A., Barlati S.
    Mol. Neurobiol. 45:61-75(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-210 AND ILE-512.

Entry informationi

Entry nameiRED2_HUMAN
AccessioniPrimary (citable) accession number: Q9NS39
Secondary accession number(s): B2RPJ5, Q5VUT6, Q5VW42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 1, 2000
Last modified: February 4, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.