Q9NS37 (ZHANG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CREB/ATF bZIP transcription factor

Alternative name(s):
Host cell factor-binding transcription factor Zhangfei
Short name=HCF-binding transcription factor Zhangfei

Gene names

Name:	CREBZF
Synonyms:	ZF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells. Ref.1 Ref.6
Subunit structure	Interacts with HCFC1; the interaction inhibits CREB3 transcriptional activity. Ref.1 Ref.6
Subcellular location	Nucleus. Note: Colocalizes in promyelocytic leukemia protein nuclear bodies (PML-NB) with CREB3 and HCFC1. Ref.6
Tissue specificity	In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver. Ref.1
Miscellaneous	Named 'Zhangfei' after a legendary Chinese warrior who was contemporary with Luman in around 220 AD.
Sequence similarities	Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Sequence caution	The sequence AAD28325.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD28325.1 differs from that shown. Reason: Frameshift at positions 5 and 49. The sequence AAZ42189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG36256.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG36256.1 differs from that shown. Reason: Frameshift at position 59.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of gene expression, epigenetic Inferred from expression pattern Ref.6. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.6. Source: UniProtKB regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.6. Source: UniProtKB response to virus Inferred from direct assay Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	DNA binding Non-traceable author statement Ref.1. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Esrrg	P62509	3	EBI-632965,EBI-5274019	From a different organism.
HCFC1	P51610	8	EBI-632965,EBI-396176
SIRT1	Q96EB6	3	EBI-632965,EBI-1802965

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	CREB/ATF bZIP transcription factor		PRO_0000076645
Regions
	Domain	204 – 267	64	bZIP
	Region	219 – 226	8	Basic motif By similarity
	Region	232 – 267	36	Leucine-zipper By similarity
	Motif	303 – 306	4	HCFC1-binding motif (HBM)
Experimental info
	Mutagenesis	303	1	D → A: Significantly reduced binding to HCFC1. Ref.1
	Mutagenesis	304	1	H → A: Significantly reduced binding to HCFC1. Ref.1
	Mutagenesis	306	1	Y → A: Does not interact with HCFC1 and is inefficient at inhibiting CREB3 transcriptional activity. Does not colocalize with CREB3 in promyelocytic leukemia protein nuclear bodies (PML-NB). Ref.1 Ref.6
	Sequence conflict	16	1	S → F in AAH60807. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9NS37 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: BE3AC77203216155	FASTA	354	37,134
	10 20 30 40 50 60 MRHSLTKLLA ASGSNSPTRS ESPEPAATCS LPSDLTRAAA GEEETAAAGS PGRKQQFGDE 70 80 90 100 110 120 GELEAGRGSR GGVAVRAPSP EEMEEEAIAS LPGEETEDMD FLSGLELADL LDPRQPDWHL 130 140 150 160 170 180 DPGLSSPGPL SSSGGGSDSG GLWRGDDDDE AAAAEMQRFS DLLQRLLNGI GGCSSSSDSG 190 200 210 220 230 240 SAEKRRRKSP GGGGGGGSGN DNNQAATKSP RKAAAAAARL NRLKKKEYVM GLESRVRGLA 250 260 270 280 290 300 AENQELRAEN RELGKRVQAL QEESRYLRAV LANETGLARL LSRLSGVGLR LTTSLFRDSP 310 320 330 340 350 AGDHDYALPV GKQKQDLLEE DDSAGGVCLH VDKDKVSVEF CSACARKASS SLKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16." Lu R., Misra V. Nucleic Acids Res. 28:2446-2454(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HCFC1, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-303; HIS-304 AND TYR-306. Tissue: Cervix carcinoma.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Placenta.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-354. Tissue: Hippocampus.
[5]	Zhang J.W., Zhang X., Ma Y.N. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-354. Tissue: Bone marrow.
[6]	"Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman." Misra V., Rapin N., Akhova O., Bainbridge M., Korchinski P. J. Biol. Chem. 280:15257-15266(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A SUPPRESSOR OF CREB3 TRANSCRIPTIONAL ACTIVITY, INTERACTION WITH HCFC1, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-306.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039942 mRNA. Translation: AAD28325.1. Sequence problems. AP000642 Genomic DNA. No translation available. BC060807 mRNA. Translation: AAH60807.1. BC093796 mRNA. Translation: AAH93796.2. BC093798 mRNA. Translation: AAH93798.2. AK313471 mRNA. Translation: BAG36256.1. Sequence problems. DQ128105 mRNA. Translation: AAZ42189.1. Different initiation.
IPI	IPI00164768.
RefSeq	NP_001034707.1. NM_001039618.2.
UniGene	Hs.535319. Hs.714910.
3D structure databases
ProteinModelPortal	Q9NS37.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NS37. 5 interactions.
MINT	MINT-1200350.
STRING	9606.ENSP00000260058.
PTM databases
PhosphoSite	Q9NS37.
Polymorphism databases
DMDM	251757415.
Proteomic databases
PaxDb	Q9NS37.
PRIDE	Q9NS37.
Protocols and materials databases
DNASU	58487.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000398294; ENSP00000381342; ENSG00000137504. ENST00000490820; ENSP00000434281; ENSG00000137504. ENST00000527447; ENSP00000433459; ENSG00000137504.
GeneID	58487.
KEGG	hsa:58487.
UCSC	uc001pas.2. human.
Organism-specific databases
CTD	58487.
GeneCards	GC11M085368.
HGNC	HGNC:24905. CREBZF.
HPA	HPA021526.
MIM	606444. gene.
neXtProt	NX_Q9NS37.
PharmGKB	PA162382821.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46652.
HOGENOM	HOG000285965.
HOVERGEN	HBG099107.
InParanoid	Q9NS37.
OrthoDB	EOG4PG61Z.
Gene expression databases
ArrayExpress	Q9NS37.
Bgee	Q9NS37.
CleanEx	HS_CREBZF.
Genevestigator	Q9NS37.
GermOnline	ENSG00000137504. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. False negative. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	58487.
NextBio	64946.
SOURCE	Search...

Entry information

Entry name

ZHANG_HUMAN

Accession

Primary (citable) accession number: Q9NS37
Secondary accession number(s): B2R8Q9, Q0P5U9, Q52LT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.