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Protein

CREB/ATF bZIP transcription factor

Gene

CREBZF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: InterPro
  • sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  • negative regulation of gene expression, epigenetic Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  • response to virus Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
CREB/ATF bZIP transcription factor
Alternative name(s):
Host cell factor-binding transcription factor Zhangfei
Short name:
HCF-binding transcription factor Zhangfei
Gene namesi
Name:CREBZF
Synonyms:ZF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:24905. CREBZF.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Colocalizes in promyelocytic leukemia protein nuclear bodies (PML-NB) with CREB3 and HCFC1.

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi303 – 3031D → A: Significantly reduced binding to HCFC1. 1 Publication
Mutagenesisi304 – 3041H → A: Significantly reduced binding to HCFC1. 1 Publication
Mutagenesisi306 – 3061Y → A: Does not interact with HCFC1 and is inefficient at inhibiting CREB3 transcriptional activity. Does not colocalize with CREB3 in promyelocytic leukemia protein nuclear bodies (PML-NB). 2 Publications

Organism-specific databases

PharmGKBiPA162382821.

Polymorphism and mutation databases

BioMutaiCREBZF.
DMDMi251757415.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354CREB/ATF bZIP transcription factorPRO_0000076645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9NS37.
PaxDbiQ9NS37.
PRIDEiQ9NS37.

PTM databases

PhosphoSiteiQ9NS37.

Expressioni

Tissue specificityi

In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver.1 Publication

Gene expression databases

BgeeiQ9NS37.
CleanExiHS_CREBZF.
ExpressionAtlasiQ9NS37. baseline and differential.
GenevisibleiQ9NS37. HS.

Organism-specific databases

HPAiHPA021526.

Interactioni

Subunit structurei

Interacts with HCFC1; the interaction inhibits CREB3 transcriptional activity.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-632965,EBI-632965
ACYP2P146213EBI-632965,EBI-10198377
ATF4P188485EBI-632965,EBI-492498
CTNNBL1Q8WYA63EBI-632965,EBI-748128
EsrrgP625093EBI-632965,EBI-5274019From a different organism.
HCFC1P516108EBI-632965,EBI-396176
SIRT1Q96EB63EBI-632965,EBI-1802965
TTC23Q5W5X93EBI-632965,EBI-6447954

Protein-protein interaction databases

BioGridi121817. 16 interactions.
DIPiDIP-33934N.
IntActiQ9NS37. 10 interactions.
MINTiMINT-1200350.
STRINGi9606.ENSP00000433459.

Structurei

3D structure databases

ProteinModelPortaliQ9NS37.
SMRiQ9NS37. Positions 209-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 26764bZIPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni219 – 2268Basic motifBy similarity
Regioni232 – 26736Leucine-zipperBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi303 – 3064HCFC1-binding motif (HBM)

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Sequence Analysis

Phylogenomic databases

eggNOGiNOG46652.
GeneTreeiENSGT00390000016589.
HOGENOMiHOG000285965.
HOVERGENiHBG099107.
InParanoidiQ9NS37.
OrthoDBiEOG7FZ00Q.
PhylomeDBiQ9NS37.
TreeFamiTF336153.

Family and domain databases

InterProiIPR004827. bZIP.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NS37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHSLTKLLA ASGSNSPTRS ESPEPAATCS LPSDLTRAAA GEEETAAAGS
60 70 80 90 100
PGRKQQFGDE GELEAGRGSR GGVAVRAPSP EEMEEEAIAS LPGEETEDMD
110 120 130 140 150
FLSGLELADL LDPRQPDWHL DPGLSSPGPL SSSGGGSDSG GLWRGDDDDE
160 170 180 190 200
AAAAEMQRFS DLLQRLLNGI GGCSSSSDSG SAEKRRRKSP GGGGGGGSGN
210 220 230 240 250
DNNQAATKSP RKAAAAAARL NRLKKKEYVM GLESRVRGLA AENQELRAEN
260 270 280 290 300
RELGKRVQAL QEESRYLRAV LANETGLARL LSRLSGVGLR LTTSLFRDSP
310 320 330 340 350
AGDHDYALPV GKQKQDLLEE DDSAGGVCLH VDKDKVSVEF CSACARKASS

SLKM
Length:354
Mass (Da):37,134
Last modified:July 7, 2009 - v2
Checksum:iBE3AC77203216155
GO

Sequence cautioni

The sequence AAD28325.1 differs from that shown. Reason: Frameshift at positions 5 and 49. Curated
The sequence AAD28325.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAZ42189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG36256.1 differs from that shown. Reason: Frameshift at position 59. Curated
The sequence BAG36256.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161S → F in AAH60807 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039942 mRNA. Translation: AAD28325.1. Sequence problems.
AP000642 Genomic DNA. No translation available.
BC060807 mRNA. Translation: AAH60807.1.
BC093796 mRNA. Translation: AAH93796.2.
BC093798 mRNA. Translation: AAH93798.2.
AK313471 mRNA. Translation: BAG36256.1. Sequence problems.
DQ128105 mRNA. Translation: AAZ42189.1. Different initiation.
CCDSiCCDS41697.1.
RefSeqiNP_001034707.1. NM_001039618.2.
XP_011543497.1. XM_011545195.1.
UniGeneiHs.535319.
Hs.714910.

Genome annotation databases

EnsembliENST00000490820; ENSP00000434281; ENSG00000137504.
ENST00000527447; ENSP00000433459; ENSG00000137504.
GeneIDi58487.
KEGGihsa:58487.
UCSCiuc001pas.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039942 mRNA. Translation: AAD28325.1. Sequence problems.
AP000642 Genomic DNA. No translation available.
BC060807 mRNA. Translation: AAH60807.1.
BC093796 mRNA. Translation: AAH93796.2.
BC093798 mRNA. Translation: AAH93798.2.
AK313471 mRNA. Translation: BAG36256.1. Sequence problems.
DQ128105 mRNA. Translation: AAZ42189.1. Different initiation.
CCDSiCCDS41697.1.
RefSeqiNP_001034707.1. NM_001039618.2.
XP_011543497.1. XM_011545195.1.
UniGeneiHs.535319.
Hs.714910.

3D structure databases

ProteinModelPortaliQ9NS37.
SMRiQ9NS37. Positions 209-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121817. 16 interactions.
DIPiDIP-33934N.
IntActiQ9NS37. 10 interactions.
MINTiMINT-1200350.
STRINGi9606.ENSP00000433459.

PTM databases

PhosphoSiteiQ9NS37.

Polymorphism and mutation databases

BioMutaiCREBZF.
DMDMi251757415.

Proteomic databases

MaxQBiQ9NS37.
PaxDbiQ9NS37.
PRIDEiQ9NS37.

Protocols and materials databases

DNASUi58487.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000490820; ENSP00000434281; ENSG00000137504.
ENST00000527447; ENSP00000433459; ENSG00000137504.
GeneIDi58487.
KEGGihsa:58487.
UCSCiuc001pas.2. human.

Organism-specific databases

CTDi58487.
GeneCardsiGC11M085368.
HGNCiHGNC:24905. CREBZF.
HPAiHPA021526.
MIMi606444. gene.
neXtProtiNX_Q9NS37.
PharmGKBiPA162382821.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG46652.
GeneTreeiENSGT00390000016589.
HOGENOMiHOG000285965.
HOVERGENiHBG099107.
InParanoidiQ9NS37.
OrthoDBiEOG7FZ00Q.
PhylomeDBiQ9NS37.
TreeFamiTF336153.

Miscellaneous databases

ChiTaRSiCREBZF. human.
GenomeRNAii58487.
NextBioi64946.
PROiQ9NS37.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NS37.
CleanExiHS_CREBZF.
ExpressionAtlasiQ9NS37. baseline and differential.
GenevisibleiQ9NS37. HS.

Family and domain databases

InterProiIPR004827. bZIP.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16."
    Lu R., Misra V.
    Nucleic Acids Res. 28:2446-2454(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HCFC1, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-303; HIS-304 AND TYR-306.
    Tissue: Cervix carcinoma.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Placenta.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-354.
    Tissue: Hippocampus.
  5. Zhang J.W., Zhang X., Ma Y.N.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-354.
    Tissue: Bone marrow.
  6. "Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman."
    Misra V., Rapin N., Akhova O., Bainbridge M., Korchinski P.
    J. Biol. Chem. 280:15257-15266(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A SUPPRESSOR OF CREB3 TRANSCRIPTIONAL ACTIVITY, INTERACTION WITH HCFC1, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-306.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiZHANG_HUMAN
AccessioniPrimary (citable) accession number: Q9NS37
Secondary accession number(s): B2R8Q9, Q0P5U9, Q52LT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 7, 2009
Last modified: July 22, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Named 'Zhangfei' after a legendary Chinese warrior who was contemporary with Luman in around 220 AD.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.