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Q9NS37 (ZHANG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CREB/ATF bZIP transcription factor
Alternative name(s):
Host cell factor-binding transcription factor Zhangfei
Short name=HCF-binding transcription factor Zhangfei
Gene names
Name:CREBZF
Synonyms:ZF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells. Ref.1 Ref.6

Subunit structure

Interacts with HCFC1; the interaction inhibits CREB3 transcriptional activity. Ref.1 Ref.6

Subcellular location

Nucleus. Note: Colocalizes in promyelocytic leukemia protein nuclear bodies (PML-NB) with CREB3 and HCFC1. Ref.6

Tissue specificity

In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver. Ref.1

Miscellaneous

Named 'Zhangfei' after a legendary Chinese warrior who was contemporary with Luman in around 220 AD.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.

Contains 1 bZIP (basic-leucine zipper) domain.

Sequence caution

The sequence AAD28325.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAD28325.1 differs from that shown. Reason: Frameshift at positions 5 and 49.

The sequence AAZ42189.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAG36256.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAG36256.1 differs from that shown. Reason: Frameshift at position 59.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354CREB/ATF bZIP transcription factor
PRO_0000076645

Regions

Domain204 – 26764bZIP
Region219 – 2268Basic motif By similarity
Region232 – 26736Leucine-zipper By similarity
Motif303 – 3064HCFC1-binding motif (HBM)

Experimental info

Mutagenesis3031D → A: Significantly reduced binding to HCFC1. Ref.1
Mutagenesis3041H → A: Significantly reduced binding to HCFC1. Ref.1
Mutagenesis3061Y → A: Does not interact with HCFC1 and is inefficient at inhibiting CREB3 transcriptional activity. Does not colocalize with CREB3 in promyelocytic leukemia protein nuclear bodies (PML-NB). Ref.1 Ref.6
Sequence conflict161S → F in AAH60807. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9NS37 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: BE3AC77203216155

FASTA35437,134
        10         20         30         40         50         60 
MRHSLTKLLA ASGSNSPTRS ESPEPAATCS LPSDLTRAAA GEEETAAAGS PGRKQQFGDE 

        70         80         90        100        110        120 
GELEAGRGSR GGVAVRAPSP EEMEEEAIAS LPGEETEDMD FLSGLELADL LDPRQPDWHL 

       130        140        150        160        170        180 
DPGLSSPGPL SSSGGGSDSG GLWRGDDDDE AAAAEMQRFS DLLQRLLNGI GGCSSSSDSG 

       190        200        210        220        230        240 
SAEKRRRKSP GGGGGGGSGN DNNQAATKSP RKAAAAAARL NRLKKKEYVM GLESRVRGLA 

       250        260        270        280        290        300 
AENQELRAEN RELGKRVQAL QEESRYLRAV LANETGLARL LSRLSGVGLR LTTSLFRDSP 

       310        320        330        340        350 
AGDHDYALPV GKQKQDLLEE DDSAGGVCLH VDKDKVSVEF CSACARKASS SLKM 

« Hide

References

« Hide 'large scale' references
[1]"Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16."
Lu R., Misra V.
Nucleic Acids Res. 28:2446-2454(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HCFC1, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-303; HIS-304 AND TYR-306.
Tissue: Cervix carcinoma.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Placenta.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-354.
Tissue: Hippocampus.
[5]Zhang J.W., Zhang X., Ma Y.N.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-354.
Tissue: Bone marrow.
[6]"Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman."
Misra V., Rapin N., Akhova O., Bainbridge M., Korchinski P.
J. Biol. Chem. 280:15257-15266(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A SUPPRESSOR OF CREB3 TRANSCRIPTIONAL ACTIVITY, INTERACTION WITH HCFC1, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-306.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039942 mRNA. Translation: AAD28325.1. Sequence problems.
AP000642 Genomic DNA. No translation available.
BC060807 mRNA. Translation: AAH60807.1.
BC093796 mRNA. Translation: AAH93796.2.
BC093798 mRNA. Translation: AAH93798.2.
AK313471 mRNA. Translation: BAG36256.1. Sequence problems.
DQ128105 mRNA. Translation: AAZ42189.1. Different initiation.
CCDSCCDS41697.1.
RefSeqNP_001034707.1. NM_001039618.2.
XP_006718708.1. XM_006718645.1.
UniGeneHs.535319.
Hs.714910.

3D structure databases

ProteinModelPortalQ9NS37.
SMRQ9NS37. Positions 214-255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121817. 12 interactions.
IntActQ9NS37. 7 interactions.
MINTMINT-1200350.
STRING9606.ENSP00000260058.

PTM databases

PhosphoSiteQ9NS37.

Polymorphism databases

DMDM251757415.

Proteomic databases

PaxDbQ9NS37.
PRIDEQ9NS37.

Protocols and materials databases

DNASU58487.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000398294; ENSP00000381342; ENSG00000137504.
ENST00000490820; ENSP00000434281; ENSG00000137504.
ENST00000527447; ENSP00000433459; ENSG00000137504.
GeneID58487.
KEGGhsa:58487.
UCSCuc001pas.2. human.

Organism-specific databases

CTD58487.
GeneCardsGC11M085368.
HGNCHGNC:24905. CREBZF.
HPAHPA021526.
MIM606444. gene.
neXtProtNX_Q9NS37.
PharmGKBPA162382821.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG46652.
HOGENOMHOG000285965.
HOVERGENHBG099107.
InParanoidQ9NS37.
OrthoDBEOG7FZ00Q.
PhylomeDBQ9NS37.
TreeFamTF336153.

Gene expression databases

ArrayExpressQ9NS37.
BgeeQ9NS37.
CleanExHS_CREBZF.
GenevestigatorQ9NS37.

Family and domain databases

InterProIPR004827. bZIP.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi58487.
NextBio64946.
PROQ9NS37.
SOURCESearch...

Entry information

Entry nameZHANG_HUMAN
AccessionPrimary (citable) accession number: Q9NS37
Secondary accession number(s): B2R8Q9, Q0P5U9, Q52LT3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM