Q9NS28 (RGS18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of G-protein signaling 18
Short name=RGS18

Gene names

Name:	RGS18
Synonyms:	RGS13

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i) alpha-1, G(i) alpha-2, G(i) alpha-3 and G(q) alpha. Ref.2 Ref.3
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in peripheral leukocytes, bone marrow, platelet, spleen and fetal liver. Ref.2 Ref.3
Sequence similarities	Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Signal transduction inhibitor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 235

235

Regulator of G-protein signaling 18

PRO_0000204227

Regions

Domain

86 – 202

117

RGS

Amino acid modifications

Modified residue

49

1

Phosphoserine Ref.8

Experimental info

Sequence conflict

226 – 227

2

DV → ML Ref.2

Secondary structure

1

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235

Helix Strand Turn

Details...

Helix

77 – 83

7

Helix

87 – 92

6

Helix

94 – 106

13

Helix

111 – 122

12

Helix

127 – 141

15

Beta strand

144 – 147

4

Helix

155 – 163

9

Helix

164 – 166

3

Turn

170 – 173

4

Helix

174 – 186

13

Helix

188 – 193

6

Helix

196 – 202

7

Sequences

	Sequence		Length	Mass (Da)
	Q9NS28 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 973ABDE8EC7DE3D5	FASTA	235	27,582
	10 20 30 40 50 60 METTLLFFSQ INMCESKEKT FFKLIHGSGK EETSKEAKIR AKEKRNRLSL LVQKPEFHED 70 80 90 100 110 120 TRSSRSGHLA KETRVSPEEA VKWGESFDKL LSHRDGLEAF TRFLKTEFSE ENIEFWIACE 130 140 150 160 170 180 DFKKSKGPQQ IHLKAKAIYE KFIQTDAPKE VNLDFHTKEV ITNSITQPTL HSFDAAQSRV 190 200 210 220 230 YQLMEQDSYT RFLKSDIYLD LMEGRPQRPT NLRRRSRSFT CNEFQDVQSD VAIWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel regulator of G-protein signaling." Zhang W., Wan T., Yuan Z., He L., Cao X. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and characterization of a novel regulator of G-protein signaling from mouse hematopoietic stem cells." Park I.K., Klug C.A., Li K., Jerabek L., Li L., Nanamori M., Neubig R.R., Hood L., Weissman I.L., Clarke M.F. J. Biol. Chem. 276:915-923(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"Cloning and characterization of a novel regulator of G protein signalling in human platelets." Gagnon A.W., Murray D.L., Leadley R.J. Jr. Cell. Signal. 14:595-606(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Platelet.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[8]	"Differential proteome analysis of TRAP-activated platelets: involvement of DOK-2 and phosphorylation of RGS proteins." Garcia A., Prabhakar S., Hughan S., Anderson T.W., Brock C.J., Pearce A.C., Dwek R.A., Watson S.P., Hebestreit H.F., Zitzmann N. Blood 103:2088-2095(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-49.
[9]	"Solution structure of the RGS domain of human regulator of G-protein signaling 18." RIKEN structural genomics initiative (RSGI) Submitted (APR-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 76-204.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF076642 mRNA. Translation: AAF80227.1.
AF268036 mRNA. Translation: AAK58589.1.
AL596342, AL513175 Genomic DNA. Translation: CAH71160.1.
AK315377 mRNA. Translation: BAG37770.1.
AL513175, AL596342 Genomic DNA. Translation: CAH70529.1.
CH471067 Genomic DNA. Translation: EAW91223.1.
BC020632 mRNA. Translation: AAH20632.1.

IPI

IPI00016825.

RefSeq

NP_570138.1. NM_130782.2.

UniGene

Hs.440890.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DLV	NMR	-	A	76-202	[»]
2JM5	NMR	-	A	75-223	[»]
2OWI	NMR	-	A	75-223	[»]

ProteinModelPortal

Q9NS28.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59096N.

STRING

9606.ENSP00000356430.

PTM databases

PhosphoSite

Q9NS28.

Polymorphism databases

DMDM

15214228.

2D gel databases

OGP

Q9NS28.

Proteomic databases

PaxDb

Q9NS28.

PRIDE

Q9NS28.

Protocols and materials databases

DNASU

64407.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000367460; ENSP00000356430; ENSG00000150681.

GeneID

64407.

KEGG

hsa:64407.

UCSC

uc001gsg.3. human.

Organism-specific databases

CTD

64407.

GeneCards

GC01P192127.

HGNC

HGNC:14261. RGS18.

HPA

HPA045780.

MIM

607192. gene.

neXtProt

NX_Q9NS28.

PharmGKB

PA34369.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG278594.

HOGENOM

HOG000233512.

HOVERGEN

HBG013233.

InParanoid

Q9NS28.

KO

K16449.

OMA

LDFHTKE.

OrthoDB

EOG42BX9H.

PhylomeDB

Q9NS28.

Gene expression databases

Bgee

Q9NS28.

CleanEx

HS_RGS13.
HS_RGS18.

Genevestigator

Q9NS28.

GermOnline

ENSG00000150681. Homo sapiens.

Family and domain databases

Gene3D

1.10.196.10. 2 hits.

InterPro

IPR000342. Regulat_G_prot_signal.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
[Graphical view]

Pfam

PF00615. RGS. 1 hit.
[Graphical view]

PRINTS

PR01301. RGSPROTEIN.

SMART

SM00315. RGS. 1 hit.
[Graphical view]

SUPFAM

SSF48097. Regulat_G_prot_signal_superfam. 1 hit.

PROSITE

PS50132. RGS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9NS28.

GenomeRNAi

64407.

NextBio

66362.

SOURCE

Search...

Entry information

Entry name

RGS18_HUMAN

Accession

Primary (citable) accession number: Q9NS28
Secondary accession number(s): B2RD23

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.