Q9NRZ5 (PLCD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase delta
EC=2.3.1.51

Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 4
Short name=1-AGP acyltransferase 4
Short name=1-AGPAT 4
Lysophosphatidic acid acyltransferase delta
Short name=LPAAT-delta

Gene names

Name:	AGPAT4
ORF Names:	UNQ499/PRO1016

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Widely expressed with highest levels in skeletal muscle, followed by heart, liver, prostate and thymus. Ref.5
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CDP-diacylglycerol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway phosphatidic acid biosynthetic process Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome triglyceride biosynthetic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	1-acylglycerol-3-phosphate O-acyltransferase activity Non-traceable author statement PubMed 15367102. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CRK	P46108	1	EBI-1754287,EBI-886

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	1-acyl-sn-glycerol-3-phosphate acyltransferase delta		PRO_0000208197
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	307 – 327	21	Helical; Potential
	Transmembrane	338 – 358	21	Helical; Potential
	Motif	96 – 101	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q9NRZ5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3EFC13D196F8CDC5	FASTA	378	44,021
	10 20 30 40 50 60 MDLAGLLKSQ FLCHLVFCYV FIASGLIINT IQLFTLLLWP INKQLFRKIN CRLSYCISSQ 70 80 90 100 110 120 LVMLLEWWSG TECTIFTDPR AYLKYGKENA IVVLNHKFEI DFLCGWSLSE RFGLLGGSKV 130 140 150 160 170 180 LAKKELAYVP IIGWMWYFTE MVFCSRKWEQ DRKTVATSLQ HLRDYPEKYF FLIHCEGTRF 190 200 210 220 230 240 TEKKHEISMQ VARAKGLPRL KHHLLPRTKG FAITVRSLRN VVSAVYDCTL NFRNNENPTL 250 260 270 280 290 300 LGVLNGKKYH ADLYVRRIPL EDIPEDDDEC SAWLHKLYQE KDAFQEEYYR TGTFPETPMV 310 320 330 340 350 360 PPRRPWTLVN WLFWASLVLY PFFQFLVSMI RSGSSLTLAS FILVFFVASV GVRWMIGVTE 370 IDKGSAYGNS DSKQKLND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The structure and functions of human lysophosphatidic acid acyltransferases." Leung D.W. Front. Biosci. 6:D944-D953(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[5]	"Enzymatic activities of the human AGPAT isoform 3 and isoform 5: localization of AGPAT5 to mitochondria." Prasad S.S., Garg A., Agarwal A.K. J. Lipid Res. 52:451-462(2011) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF156776 mRNA. Translation: AAF80338.1. AY358506 mRNA. Translation: AAQ88870.1. AL109942 Genomic DNA. Translation: CAI21478.1. BC020209 mRNA. Translation: AAH20209.1.
IPI	IPI00016956.
RefSeq	NP_064518.1. NM_020133.2.
UniGene	Hs.353175.
3D structure databases
ProteinModelPortal	Q9NRZ5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NRZ5. 1 interaction.
STRING	9606.ENSP00000314036.
PTM databases
PhosphoSite	Q9NRZ5.
Polymorphism databases
DMDM	12230468.
Proteomic databases
PaxDb	Q9NRZ5.
PRIDE	Q9NRZ5.
Protocols and materials databases
DNASU	56895.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000320285; ENSP00000314036; ENSG00000026652.
GeneID	56895.
KEGG	hsa:56895.
UCSC	uc003qtr.1. human.
Organism-specific databases
CTD	56895.
GeneCards	GC06M161471.
HGNC	HGNC:20885. AGPAT4.
HPA	HPA053287.
MIM	614795. gene.
neXtProt	NX_Q9NRZ5.
PharmGKB	PA134961047.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0204.
HOGENOM	HOG000006110.
HOVERGEN	HBG008205.
InParanoid	Q9NRZ5.
KO	K13523.
OMA	FFLIHCE.
OrthoDB	EOG4W9J46.
PhylomeDB	Q9NRZ5.
Enzyme and pathway databases
BioCyc	MetaCyc:HS00452-MONOMER.
Reactome	REACT_111217. Metabolism.
UniPathway	UPA00557; UER00613.
Gene expression databases
ArrayExpress	Q9NRZ5.
Bgee	Q9NRZ5.
CleanEx	HS_AGPAT4.
Genevestigator	Q9NRZ5.
GermOnline	ENSG00000026652. Homo sapiens.
Family and domain databases
InterPro	IPR002123. Plipid/glycerol_acylTrfase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AGPAT4. human.
GenomeRNAi	56895.
NextBio	62325.
SOURCE	Search...

Entry information

Entry name

PLCD_HUMAN

Accession

Primary (citable) accession number: Q9NRZ5
Secondary accession number(s): Q5TEF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.