Q9NRY7 (PLS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipid scramblase 2
Short name=PL scramblase 2

Alternative name(s):
Ca(2+)-dependent phospholipid scramblase 2

Gene names

Name:

PLSCR2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
Cofactor	Calcium By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Exclusively expressed in testis.
Sequence similarities	Belongs to the phospholipid scramblase family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phospholipid scrambling Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	calcium ion binding Non-traceable author statement Ref.1. Source: UniProtKB phospholipid scramblase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NRY7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NRY7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRSWNSLFCLNSSRPPGHIVYPKHQAGHTGKQADHLGSQAFYPGRQHDYLVPPAGTAGIPVQNQPGRPEGVPWM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Phospholipid scramblase 2		PRO_0000100787
Regions
	Topological domain	1 – 203	203	Cytoplasmic By similarity
	Transmembrane	204 – 220	17	Helical; Potential
	Topological domain	221 – 224	4	Extracellular By similarity
	Compositional bias	2 – 7	6	Poly-Pro
	Compositional bias	96 – 104	9	Cys-rich
Amino acid modifications
	Modified residue	76	1	Phosphothreonine; by PKC By similarity
	Lipidation	149	1	S-palmitoyl cysteine Probable
	Lipidation	152	1	S-palmitoyl cysteine Probable
	Lipidation	154	1	S-palmitoyl cysteine Probable
Natural variations
	Alternative sequence	1	1	M → MRSWNSLFCLNSSRPPGHIV YPKHQAGHTGKQADHLGSQA FYPGRQHDYLVPPAGTAGIP VQNQPGRPEGVPWM in isoform 2.		VSP_045894
Experimental info
	Sequence conflict	199 – 201	3	DLD → NLN in BAG63335. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FD218C63C59D239A	FASTA	224	25,523
	10 20 30 40 50 60 MPAPPPPLNC PPGLEYLSQI DMILIHQQIE LLEVLFSFES SNMYEIKNSF GQRIYFAAED 70 80 90 100 110 120 TNFCIRNCCG RSRPFTLRIT DNVGREVITL ERPLRCNCCC CPCCLQEIEI QAPPGVPVGY 130 140 150 160 170 180 VTQTWHPCLT KFTIKNQKRE DVLKISGPCI VCSCIAGVDF EITSLDEQIV VGRISKHWSG 190 200 210 220 FLREAFTDAD NFGIQFPRDL DVKMKAVMIG ACFLIDYMFF ERTR
	Isoform 2 [UniParc]. Checksum: 58F50C24AB438729	FASTA	297	33,504
	10 20 30 40 50 60 MRSWNSLFCL NSSRPPGHIV YPKHQAGHTG KQADHLGSQA FYPGRQHDYL VPPAGTAGIP 70 80 90 100 110 120 VQNQPGRPEG VPWMPAPPPP LNCPPGLEYL SQIDMILIHQ QIELLEVLFS FESSNMYEIK 130 140 150 160 170 180 NSFGQRIYFA AEDTNFCIRN CCGRSRPFTL RITDNVGREV ITLERPLRCN CCCCPCCLQE 190 200 210 220 230 240 IEIQAPPGVP VGYVTQTWHP CLTKFTIKNQ KREDVLKISG PCIVCSCIAG VDFEITSLDE 250 260 270 280 290 QIVVGRISKH WSGFLREAFT DADNFGIQFP RDLDVKMKAV MIGACFLIDY MFFERTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of three new members of the phospholipid scramblase gene family." Wiedmer T., Zhou Q., Kwoh D.Y., Sims P.J. Biochim. Biophys. Acta 1467:244-253(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CALCIUM-BINDING.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Web resources

Wikipedia

Scramblase entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF159441 mRNA. Translation: AAF91082.1. AK301909 mRNA. Translation: BAG63335.1. AC069528 Genomic DNA. No translation available. BC069785 mRNA. Translation: AAH69785.2. BC120969 mRNA. Translation: AAI20970.1. BC141969 mRNA. Translation: AAI41970.1.
IPI	IPI00016940. IPI00946560.
RefSeq	NP_001186907.1. NM_001199978.1.
UniGene	Hs.177968.
3D structure databases
ProteinModelPortal	Q9NRY7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NRY7. 1 interaction.
Polymorphism databases
DMDM	14548198.
Proteomic databases
PaxDb	Q9NRY7.
PRIDE	Q9NRY7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336685; ENSP00000338707; ENSG00000163746. ENST00000497985; ENSP00000420132; ENSG00000163746.
GeneID	57047.
KEGG	hsa:57047.
UCSC	uc003evv.2. human.
Organism-specific databases
CTD	57047.
GeneCards	GC03M146110.
H-InvDB	HIX0200552.
HGNC	HGNC:16494. PLSCR2.
HPA	HPA051352.
MIM	607610. gene.
neXtProt	NX_Q9NRY7.
PharmGKB	PA33420.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG119855.
HOGENOM	HOG000237356.
HOVERGEN	HBG019157.
InParanoid	Q9NRY7.
PhylomeDB	Q9NRY7.
Gene expression databases
ArrayExpress	Q9NRY7.
Bgee	Q9NRY7.
CleanEx	HS_PLSCR2.
Genevestigator	Q9NRY7.
GermOnline	ENSG00000163746. Homo sapiens.
Family and domain databases
InterPro	IPR005552. Scramblase. [Graphical view]
PANTHER	PTHR23248. PTHR23248. 1 hit.
Pfam	PF03803. Scramblase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57047.
NextBio	35535500.
SOURCE	Search...

Entry information

Entry name

PLS2_HUMAN

Accession

Primary (citable) accession number: Q9NRY7
Secondary accession number(s): B4DXC3 , J3KR76, Q0VAQ1, Q6NSW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.