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Q9NRY7

- PLS2_HUMAN

UniProt

Q9NRY7 - PLS2_HUMAN

Protein

Phospholipid scramblase 2

Gene

PLSCR2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (09 Jul 2014)
      Previous versions | rss
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    Functioni

    May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
    Isoform 1 has no prospholipid scramblase activity, due to the lack of a N-terminal proline-rich domain.

    Cofactori

    Calcium.By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. phospholipid scramblase activity Source: UniProtKB

    GO - Biological processi

    1. phospholipid scrambling Source: UniProtKB

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipid scramblase 2
    Short name:
    PL scramblase 2
    Alternative name(s):
    Ca(2+)-dependent phospholipid scramblase 2
    Gene namesi
    Name:PLSCR2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:16494. PLSCR2.

    Subcellular locationi

    Membrane By similarity; Single-pass type II membrane protein By similarity
    Isoform 1 : Nucleus 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33420.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Phospholipid scramblase 2PRO_0000100787Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei149 – 1491Phosphothreonine; by PKCBy similarity
    Lipidationi222 – 2221S-palmitoyl cysteineCurated
    Lipidationi225 – 2251S-palmitoyl cysteineCurated
    Lipidationi227 – 2271S-palmitoyl cysteineCurated

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ9NRY7.
    PRIDEiQ9NRY7.

    Expressioni

    Tissue specificityi

    Expression of isoform 1 seems restricted to testis.1 Publication

    Gene expression databases

    ArrayExpressiQ9NRY7.
    BgeeiQ9NRY7.
    CleanExiHS_PLSCR2.
    GenevestigatoriQ9NRY7.

    Organism-specific databases

    HPAiHPA051352.

    Interactioni

    Protein-protein interaction databases

    BioGridi121340. 1 interaction.
    IntActiQ9NRY7. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NRY7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 276276CytoplasmicBy similarityAdd
    BLAST
    Topological domaini294 – 2974ExtracellularBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei277 – 29317HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7272Proline-rich domain (PRD)By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 806Poly-Pro
    Compositional biasi169 – 1779Cys-rich

    Domaini

    The N-terminal proline-rich domain (PRD) is required for phospholipid scramblase activity.By similarity

    Sequence similaritiesi

    Belongs to the phospholipid scramblase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG119855.
    HOGENOMiHOG000237356.
    HOVERGENiHBG019157.
    InParanoidiQ9NRY7.
    OMAiPLRCNCC.
    PhylomeDBiQ9NRY7.
    TreeFamiTF314939.

    Family and domain databases

    InterProiIPR005552. Scramblase.
    [Graphical view]
    PANTHERiPTHR23248. PTHR23248. 1 hit.
    PfamiPF03803. Scramblase. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: Q9NRY7-2) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSWNSLFCL NSSRPPGHIV YPKHQAGHTG KQADHLGSQA FYPGRQHDYL    50
    VPPAGTAGIP VQNQPGRPEG VPWMPAPPPP LNCPPGLEYL SQIDMILIHQ 100
    QIELLEVLFS FESSNMYEIK NSFGQRIYFA AEDTNFCIRN CCGRSRPFTL 150
    RITDNVGREV ITLERPLRCN CCCCPCCLQE IEIQAPPGVP VGYVTQTWHP 200
    CLTKFTIKNQ KREDVLKISG PCIVCSCIAG VDFEITSLDE QIVVGRISKH 250
    WSGFLREAFT DADNFGIQFP RDLDVKMKAV MIGACFLIDY MFFERTR 297

    Note: No experimental confirmation available.

    Length:297
    Mass (Da):33,504
    Last modified:July 9, 2014 - v2
    Checksum:i58F50C24AB438729
    GO
    Isoform 1 (identifier: Q9NRY7-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-73: Missing.

    Show »
    Length:224
    Mass (Da):25,523
    Checksum:iFD218C63C59D239A
    GO
    Isoform 3 (identifier: Q9NRY7-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-14: MRSWNSLFCLNSSR → MKPFQIHLPG

    Note: No experimental confirmation available.

    Show »
    Length:293
    Mass (Da):32,970
    Checksum:iD4FFE4925247787F
    GO

    Sequence cautioni

    The sequence AAH55415.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti272 – 2743DLD → NLN in BAG63335. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7373Missing in isoform 1. 2 PublicationsVSP_055239Add
    BLAST
    Alternative sequencei1 – 1414MRSWN…LNSSR → MKPFQIHLPG in isoform 3. 1 PublicationVSP_055240Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF159441 mRNA. Translation: AAF91082.1.
    AK301909 mRNA. Translation: BAG63335.1.
    AC069528 Genomic DNA. No translation available.
    BC055415 mRNA. Translation: AAH55415.1. Different initiation.
    BC069785 mRNA. Translation: AAH69785.2.
    BC120969 mRNA. Translation: AAI20970.1.
    BC141969 mRNA. Translation: AAI41970.1.
    CCDSiCCDS56284.1. [Q9NRY7-2]
    RefSeqiNP_001186907.1. NM_001199978.1. [Q9NRY7-2]
    NP_001186908.1. NM_001199979.1.
    XP_006713775.1. XM_006713712.1. [Q9NRY7-1]
    UniGeneiHs.744414.

    Genome annotation databases

    EnsembliENST00000336685; ENSP00000338707; ENSG00000163746. [Q9NRY7-1]
    ENST00000497985; ENSP00000420132; ENSG00000163746. [Q9NRY7-2]
    GeneIDi57047.
    KEGGihsa:57047.
    UCSCiuc003evv.2. human. [Q9NRY7-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Scramblase entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF159441 mRNA. Translation: AAF91082.1 .
    AK301909 mRNA. Translation: BAG63335.1 .
    AC069528 Genomic DNA. No translation available.
    BC055415 mRNA. Translation: AAH55415.1 . Different initiation.
    BC069785 mRNA. Translation: AAH69785.2 .
    BC120969 mRNA. Translation: AAI20970.1 .
    BC141969 mRNA. Translation: AAI41970.1 .
    CCDSi CCDS56284.1. [Q9NRY7-2 ]
    RefSeqi NP_001186907.1. NM_001199978.1. [Q9NRY7-2 ]
    NP_001186908.1. NM_001199979.1.
    XP_006713775.1. XM_006713712.1. [Q9NRY7-1 ]
    UniGenei Hs.744414.

    3D structure databases

    ProteinModelPortali Q9NRY7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121340. 1 interaction.
    IntActi Q9NRY7. 1 interaction.

    Proteomic databases

    PaxDbi Q9NRY7.
    PRIDEi Q9NRY7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336685 ; ENSP00000338707 ; ENSG00000163746 . [Q9NRY7-1 ]
    ENST00000497985 ; ENSP00000420132 ; ENSG00000163746 . [Q9NRY7-2 ]
    GeneIDi 57047.
    KEGGi hsa:57047.
    UCSCi uc003evv.2. human. [Q9NRY7-2 ]

    Organism-specific databases

    CTDi 57047.
    GeneCardsi GC03M146110.
    H-InvDB HIX0200552.
    HGNCi HGNC:16494. PLSCR2.
    HPAi HPA051352.
    MIMi 607610. gene.
    neXtProti NX_Q9NRY7.
    PharmGKBi PA33420.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG119855.
    HOGENOMi HOG000237356.
    HOVERGENi HBG019157.
    InParanoidi Q9NRY7.
    OMAi PLRCNCC.
    PhylomeDBi Q9NRY7.
    TreeFami TF314939.

    Miscellaneous databases

    GeneWikii PLSCR2.
    GenomeRNAii 57047.
    NextBioi 35535500.
    PROi Q9NRY7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NRY7.
    Bgeei Q9NRY7.
    CleanExi HS_PLSCR2.
    Genevestigatori Q9NRY7.

    Family and domain databases

    InterProi IPR005552. Scramblase.
    [Graphical view ]
    PANTHERi PTHR23248. PTHR23248. 1 hit.
    Pfami PF03803. Scramblase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of three new members of the phospholipid scramblase gene family."
      Wiedmer T., Zhou Q., Kwoh D.Y., Sims P.J.
      Biochim. Biophys. Acta 1467:244-253(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CALCIUM-BINDING, TISSUE SPECIFICITY.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    3. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Testis.
    5. "N-terminal proline-rich domain is required for scrambling activity of human phospholipid scramblases."
      Rayala S., Francis V.G., Sivagnanam U., Gummadi S.N.
      J. Biol. Chem. 289:13206-13218(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION (ISOFORM 1), ABSENCE OF SCRAMBLASE ACTIVITY (ISOFORM 1), CALCIUM-BINDING, SUBCELLULAR LOCATION (ISOFORM 1).

    Entry informationi

    Entry nameiPLS2_HUMAN
    AccessioniPrimary (citable) accession number: Q9NRY7
    Secondary accession number(s): B4DXC3
    , J3KR76, Q0VAQ1, Q6NSW9, Q7Z4L7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: July 9, 2014
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3