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Q9NRY7 (PLS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipid scramblase 2

Short name=PL scramblase 2
Alternative name(s):
Ca(2+)-dependent phospholipid scramblase 2
Gene names
Name:PLSCR2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length297 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Ref.5

Isoform 1 has no prospholipid scramblase activity, due to the lack of a N-terminal proline-rich domain. Ref.5

Cofactor

Calcium By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity Ref.5.

Isoform 1: Nucleus Ref.5.

Tissue specificity

Expression of isoform 1 seems restricted to testis. Ref.1

Domain

The N-terminal proline-rich domain (PRD) is required for phospholipid scramblase activity By similarity.

Sequence similarities

Belongs to the phospholipid scramblase family.

Sequence caution

The sequence AAH55415.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q9NRY7-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 1 (identifier: Q9NRY7-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
Isoform 3 (identifier: Q9NRY7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MRSWNSLFCLNSSR → MKPFQIHLPG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 297297Phospholipid scramblase 2
PRO_0000100787

Regions

Topological domain1 – 276276Cytoplasmic By similarity
Transmembrane277 – 29317Helical; Potential
Topological domain294 – 2974Extracellular By similarity
Region1 – 7272Proline-rich domain (PRD) By similarity
Compositional bias75 – 806Poly-Pro
Compositional bias169 – 1779Cys-rich

Amino acid modifications

Modified residue1491Phosphothreonine; by PKC By similarity
Lipidation2221S-palmitoyl cysteine Probable
Lipidation2251S-palmitoyl cysteine Probable
Lipidation2271S-palmitoyl cysteine Probable

Natural variations

Alternative sequence1 – 7373Missing in isoform 1.
VSP_055239
Alternative sequence1 – 1414MRSWN…LNSSR → MKPFQIHLPG in isoform 3.
VSP_055240

Experimental info

Sequence conflict272 – 2743DLD → NLN in BAG63335. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified July 9, 2014. Version 2.
Checksum: 58F50C24AB438729

FASTA29733,504
        10         20         30         40         50         60 
MRSWNSLFCL NSSRPPGHIV YPKHQAGHTG KQADHLGSQA FYPGRQHDYL VPPAGTAGIP 

        70         80         90        100        110        120 
VQNQPGRPEG VPWMPAPPPP LNCPPGLEYL SQIDMILIHQ QIELLEVLFS FESSNMYEIK 

       130        140        150        160        170        180 
NSFGQRIYFA AEDTNFCIRN CCGRSRPFTL RITDNVGREV ITLERPLRCN CCCCPCCLQE 

       190        200        210        220        230        240 
IEIQAPPGVP VGYVTQTWHP CLTKFTIKNQ KREDVLKISG PCIVCSCIAG VDFEITSLDE 

       250        260        270        280        290 
QIVVGRISKH WSGFLREAFT DADNFGIQFP RDLDVKMKAV MIGACFLIDY MFFERTR 

« Hide

Isoform 1 [UniParc].

Checksum: FD218C63C59D239A
Show »

FASTA22425,523
Isoform 3 [UniParc].

Checksum: D4FFE4925247787F
Show »

FASTA29332,970

References

« Hide 'large scale' references
[1]"Identification of three new members of the phospholipid scramblase gene family."
Wiedmer T., Zhou Q., Kwoh D.Y., Sims P.J.
Biochim. Biophys. Acta 1467:244-253(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CALCIUM-BINDING, TISSUE SPECIFICITY.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[3]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Testis.
[5]"N-terminal proline-rich domain is required for scrambling activity of human phospholipid scramblases."
Rayala S., Francis V.G., Sivagnanam U., Gummadi S.N.
J. Biol. Chem. 289:13206-13218(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION (ISOFORM 1), ABSENCE OF SCRAMBLASE ACTIVITY (ISOFORM 1), CALCIUM-BINDING, SUBCELLULAR LOCATION (ISOFORM 1).

Web resources

Wikipedia

Scramblase entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF159441 mRNA. Translation: AAF91082.1.
AK301909 mRNA. Translation: BAG63335.1.
AC069528 Genomic DNA. No translation available.
BC055415 mRNA. Translation: AAH55415.1. Different initiation.
BC069785 mRNA. Translation: AAH69785.2.
BC120969 mRNA. Translation: AAI20970.1.
BC141969 mRNA. Translation: AAI41970.1.
CCDSCCDS56284.1. [Q9NRY7-2]
RefSeqNP_001186907.1. NM_001199978.1. [Q9NRY7-2]
XP_006713775.1. XM_006713712.1. [Q9NRY7-1]
UniGeneHs.744414.

3D structure databases

ProteinModelPortalQ9NRY7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121340. 1 interaction.
IntActQ9NRY7. 1 interaction.

Proteomic databases

PaxDbQ9NRY7.
PRIDEQ9NRY7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336685; ENSP00000338707; ENSG00000163746. [Q9NRY7-1]
ENST00000497985; ENSP00000420132; ENSG00000163746. [Q9NRY7-2]
GeneID57047.
KEGGhsa:57047.
UCSCuc003evv.2. human. [Q9NRY7-1]

Organism-specific databases

CTD57047.
GeneCardsGC03M146110.
H-InvDBHIX0200552.
HGNCHGNC:16494. PLSCR2.
HPAHPA051352.
MIM607610. gene.
neXtProtNX_Q9NRY7.
PharmGKBPA33420.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG119855.
HOGENOMHOG000237356.
HOVERGENHBG019157.
InParanoidQ9NRY7.
OMAPLRCNCC.
PhylomeDBQ9NRY7.
TreeFamTF314939.

Gene expression databases

ArrayExpressQ9NRY7.
BgeeQ9NRY7.
CleanExHS_PLSCR2.
GenevestigatorQ9NRY7.

Family and domain databases

InterProIPR005552. Scramblase.
[Graphical view]
PANTHERPTHR23248. PTHR23248. 1 hit.
PfamPF03803. Scramblase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPLSCR2.
GenomeRNAi57047.
NextBio35535500.
PROQ9NRY7.
SOURCESearch...

Entry information

Entry namePLS2_HUMAN
AccessionPrimary (citable) accession number: Q9NRY7
Secondary accession number(s): B4DXC3 expand/collapse secondary AC list , J3KR76, Q0VAQ1, Q6NSW9, Q7Z4L7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 9, 2014
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM