Q9NRW7 (VPS45_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 45
Short name=h-VPS45
Short name=hlVps45

Gene names

Name:	VPS45
Synonyms:	VPS45A, VPS45B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.
Subunit structure	Interacts with STX6 By similarity. Interacts with ZFYVE20. Ref.8 Ref.9
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network By similarity.
Tissue specificity	Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome intracellular protein transport Non-traceable author statement Ref.2. Source: UniProtKB vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from sequence or structural similarity. Source: BHF-UCL Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome membrane Traceable author statement. Source: Reactome integral to membrane Inferred from sequence or structural similarity. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Vacuolar protein sorting-associated protein 45		PRO_0000206312
Experimental info
	Sequence conflict	81 – 82	2	YI → SL in AAC50931. Ref.1
	Sequence conflict	92	1	T → S in AAC50931. Ref.1
	Sequence conflict	188	1	A → G in AAC50931. Ref.1
	Sequence conflict	196	1	T → S in AAC50931. Ref.1
	Sequence conflict	219	1	C → L in CAB40417. Ref.2
	Sequence conflict	241	1	G → D in AAC50931. Ref.1
	Sequence conflict	298	1	K → R in AAC50931. Ref.1
	Sequence conflict	370	1	I → V in AAC50931. Ref.1
	Sequence conflict	385	1	A → V in AAC50931. Ref.1
	Sequence conflict	407 – 408	2	MM → IV in AAC50931. Ref.1
	Sequence conflict	412	1	N → S in AAC50931. Ref.1
	Sequence conflict	416	1	S → A in AAC50931. Ref.1
	Sequence conflict	481	1	R → K in AAC50931. Ref.1
	Sequence conflict	537	1	V → I in AAC50931. Ref.1
	Sequence conflict	557	1	K → R in AAC50931. Ref.1
	Sequence conflict	562	1	V → A in AAC50931. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NRW7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5574F0BE3A7D4EB3	FASTA	570	65,077
	10 20 30 40 50 60 MNVVFAVKQY ISKMIEDSGP GMKVLLMDKE TTGIVSMVYT QSEILQKEVY LFERIDSQNR 70 80 90 100 110 120 EIMKHLKAIC FLRPTKENVD YIIQELRRPK YTIYFIYFSN VISKSDVKSL AEADEQEVVA 130 140 150 160 170 180 EVQEFYGDYI AVNPHLFSLN ILGCCQGRNW DPAQLSRTTQ GLTALLLSLK KCPMIRYQLS 190 200 210 220 230 240 SEAAKRLAEC VKQVITKEYE LFEFRRTEVP PLLLILDRCD DAITPLLNQW TYQAMVHELL 250 260 270 280 290 300 GINNNRIDLS RVPGISKDLR EVVLSAENDE FYANNMYLNF AEIGSNIKNL MEDFQKKKPK 310 320 330 340 350 360 EQQKLESIAD MKAFVENYPQ FKKMSGTVSK HVTVVGELSR LVSERNLLEV SEVEQELACQ 370 380 390 400 410 420 NDHSSALQNI KRLLQNPKVT EFDAARLVML YALHYERHSS NSLPGLMMDL RNKGVSEKYR 430 440 450 460 470 480 KLVSAVVEYG GKRVRGSDLF SPKDAVAITK QFLKGLKGVE NVYTQHQPFL HETLDHLIKG 490 500 510 520 530 540 RLKENLYPYL GPSTLRDRPQ DIIVFVIGGA TYEEALTVYN LNRTTPGVRI VLGGTTVHNT 550 560 570 KSFLEEVLAS GLHSRSKESS QVTSRSASRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian homologues of yeast vacuolar protein sorting (vps) genes implicated in Golgi-to-lysosome trafficking." Pevsner J., Hsu S.-C., Hyde P.S., Scheller R.H. Gene 183:7-14(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (hlVps45)." Rajasekariah P., Eyre H.J., Stanley K.K., Walls R.S., Sutherland G.R. Int. J. Biochem. Cell Biol. 31:683-694(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leukocyte.
[3]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hematopoietic stem cell.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Hippocampus.
[8]	"Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." Nielsen E., Christoforidis S., Uttenweiler-Joseph S., Miaczynska M., Dewitte F., Wilm M., Hoflack B., Zerial M. J. Cell Biol. 151:601-612(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZFYVE20.
[9]	"Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes." de Renzis S., Soennichsen B., Zerial M. Nat. Cell Biol. 4:124-133(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZFYVE20.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35246 mRNA. Translation: AAC50931.1. AJ133421 mRNA. Translation: CAB40417.1. AF165513 mRNA. Translation: AAF86643.1. AK023170 mRNA. Translation: BAB14443.1. AL358073 Genomic DNA. Translation: CAI14265.1. CH471121 Genomic DNA. Translation: EAW53584.1. CH471121 Genomic DNA. Translation: EAW53585.1. BC012932 mRNA. Translation: AAH12932.1. BC028382 mRNA. Translation: AAH28382.1.
IPI	IPI00090327.
PIR	JC5722.
RefSeq	NP_009190.2. NM_007259.3.
UniGene	Hs.443750.
3D structure databases
ProteinModelPortal	Q9NRW7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000358126.
PTM databases
PhosphoSite	Q9NRW7.
Polymorphism databases
DMDM	23396937.
Proteomic databases
PaxDb	Q9NRW7.
PRIDE	Q9NRW7.
Protocols and materials databases
DNASU	11311.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000369130; ENSP00000358126; ENSG00000136631.
GeneID	11311.
KEGG	hsa:11311.
UCSC	uc001etp.3. human.
Organism-specific databases
CTD	11311.
GeneCards	GC01P150039.
HGNC	HGNC:14579. VPS45.
HPA	HPA027425. HPA027441.
MIM	610035. gene.
neXtProt	NX_Q9NRW7.
PharmGKB	PA37901.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5158.
HOGENOM	HOG000200803.
HOVERGEN	HBG059810.
InParanoid	Q9NRW7.
KO	K12479.
OMA	YQLSSDM.
OrthoDB	EOG4H463H.
PhylomeDB	Q9NRW7.
Enzyme and pathway databases
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q9NRW7.
Bgee	Q9NRW7.
CleanEx	HS_VPS45.
Genevestigator	Q9NRW7.
GermOnline	ENSG00000136631. Homo sapiens.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
PANTHER	PTHR11679. PTHR11679. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
PIRSF	PIRSF005715. VPS45_Sec1. 1 hit.
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	11311.
NextBio	42971.
SOURCE	Search...

Entry information

Entry name

VPS45_HUMAN

Accession

Primary (citable) accession number: Q9NRW7
Secondary accession number(s): D3DUZ9 , Q15715, Q5T4P6, Q9Y4Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.