Reviewed, UniProtKB/Swiss-Prot Q9NRW4 (DUS22_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 22
    EC=3.1.3.48
    EC=3.1.3.16
Alternative name(s):
    JNK-stimulatory phosphatase-1
      Short name=JSP-1
    Mitogen-activated protein kinase phosphatase x
    LMW-DSP2

Gene names

Name:	DUSP22
Synonyms:	JSP1, MKPX

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	apoptosis Traceable author statement. Source: ProtInc cell proliferation Traceable author statement. Source: ProtInc inactivation of MAPK activity Traceable author statement. Source: ProtInc multicellular organismal development Traceable author statement. Source: ProtInc protein amino acid dephosphorylation Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NRW4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NRW4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     170-184: AAPGILKFWAFLRRL → GKYKEQGRTEPQPGARRWSSFPALAPLTYDNYTTET

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 184

184

Dual specificity protein phosphatase 22

PRO_0000244751

Regions

Domain

65 – 133

69

Tyrosine-protein phosphatase

Sites

Active site

88

1

Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence

170 – 184

15

AAPGI…FLRRL → GKYKEQGRTEPQPGARRWSS FPALAPLTYDNYTTET in isoform 2.

VSP_019614

Natural variant

119

1

R → H: dbSNP rs7768224.

VAR_026912

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

184

Helix Strand Turn

Details...

Beta strand

6 – 9

4

Beta strand

12 – 15

4

Helix

19 – 21

3

Helix

23 – 28

6

Beta strand

31 – 36

6

Beta strand

49 – 52

4

Helix

64 – 66

3

Helix

67 – 79

13

Beta strand

83 – 87

5

Beta strand

89 – 93

5

Helix

94 – 105

12

Helix

111 – 121

11

Helix

129 – 141

13

Helix

143 – 153

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B3F962A087C2BA20	FASTA	184	20,910
	10 20 30 40 50 60 MGNGMNKILP GLYIGNFKDA RDAEQLSKNK VTHILSVHDS ARPMLEGVKY LCIPAADSPS 70 80 90 100 110 120 QNLTRHFKES IKFIHECRLR GESCLVHCLA GVSRSVTLVI AYIMTVTDFG WEDALHTVRA 130 140 150 160 170 180 GRSCANPNVG FQRQLQEFEK HEVHQYRQWL KEEYGESPLQ DAEEAKNILA APGILKFWAF LRRL
	Isoform 2. Checksum: 48334A97991FCFF8	FASTA	205	23,268
	10 20 30 40 50 60 MGNGMNKILP GLYIGNFKDA RDAEQLSKNK VTHILSVHDS ARPMLEGVKY LCIPAADSPS 70 80 90 100 110 120 QNLTRHFKES IKFIHECRLR GESCLVHCLA GVSRSVTLVI AYIMTVTDFG WEDALHTVRA 130 140 150 160 170 180 GRSCANPNVG FQRQLQEFEK HEVHQYRQWL KEEYGESPLQ DAEEAKNILG KYKEQGRTEP 190 200 QPGARRWSSF PALAPLTYDN YTTET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of the Jnk signaling pathway by a dual-specificity phosphatase, JSP-1." Shen Y., Luche R., Wei B., Gordon M.L., Diltz C.D., Tonks N.K. Proc. Natl. Acad. Sci. U.S.A. 98:13613-13618(2001) [PubMed: 11717427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION OF ISOFORM 2, FUNCTION, TISSUE SPECIFICITY.
[2]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndromes patient." Gu J., Huang Q., Yu Y., Xu S., Wang Y., Han Z., Chen Z., Zhou J., Tu Y., Gu W., Fu G., Huang C. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hematopoietic stem cell.
[3]	"Cloning and characterization of human LMW-DSP2 gene." Mao Y., Xie Y., Cheng H. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thalamus.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Pancreas.
[7]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 56-184 (ISOFORM 2). Tissue: Myeloid.
[8]	"Crystal structure of novel human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 A resolution." Yokota T., Kashima A., Kato R., Sugio S. Submitted (OCT-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 1-163.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF424702 mRNA. Translation: AAL18850.1.
AF165519 mRNA. Translation: AAF86649.1.
AY249859 mRNA. Translation: AAP76376.1.
AK296402 mRNA. Translation: BAG59068.1.
AL365272 Genomic DNA. Translation: CAH72534.1.
AL365272 Genomic DNA. Translation: CAH72535.1.
BC016844 mRNA. Translation: AAH16844.1. Different initiation.
BC022847 mRNA. Translation: AAH22847.1.
AB208997 mRNA. Translation: BAD92234.1.

IPI

IPI00024617.
IPI00646309.

RefSeq

NP_064570.1.

UniGene

Hs.29106

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WRM	X-ray	1.50	A	1-163	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q9NRW4.

Genome annotation databases

Ensembl

ENSG00000112679. Homo sapiens. [Contig view]

GeneID

56940.

KEGG

hsa:56940.

Organism-specific databases

GeneCards

GC06P000237.

H-InvDB

HIX0005513.

HGNC

HGNC:16077. DUSP22.

PharmGKB

PA134991025.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9NRW4.

HOVERGEN

Q9NRW4.

OMA

Q9NRW4. KHEVHQY.

Enzyme and pathway databases

BRENDA

3.1.3.16. 247.
3.1.3.48. 247.

Gene expression databases

ArrayExpress

Q9NRW4.

Bgee

Q9NRW4.

CleanEx

HS_DUSP22.

GermOnline

ENSG00000112679. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Tyr_Pase.
IPR016130. Tyr_Pase_AS.
IPR000340. Tyr_Pase_dual_specific.
[Graphical view]

Pfam

PF00782. DSPc. 1 hit.
[Graphical view]

SMART

SM00195. DSPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. False negative.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

62505.

Entry information

Entry name

DUS22_HUMAN

Accession

Primary (citable) accession number: Q9NRW4
Secondary accession number(s): B4DK56 , Q59GW2, Q5VWR2, Q96AR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)