Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9NRW1 (RAB6B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-6B
Gene names
Name:RAB6B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. Ref.8

Subunit structure

Interacts with CCDC64/BICDR1; leads to its accumulation in the pericentrosomal region By similarity. Interacts with RAB6KIFL. Interacts (GTP-bound) with BICD1 (via C-terminus); the interaction is direct. Interacts (GDP-bound) with DYNLRB1. Interacts (GTP-bound) with APBA1/MINT1 isoform 2 also called Mint1_826, but not with isoform 1 Ref.8 Ref.9 Ref.12

Subcellular location

Golgi apparatus membrane; Lipid-anchor. Cytoplasmic vesicle. Note: Colocalizes with BICD1 at vesicular structures that align along microtubules. Ref.8 Ref.9

Tissue specificity

Predominantly expressed in brain.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

APBA1Q02410-24EBI-1760079,EBI-9247455

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 208207Ras-related protein Rab-6B
PRO_0000121115

Regions

Nucleotide binding20 – 278GTP
Nucleotide binding68 – 725GTP
Nucleotide binding126 – 1294GTP
Motif42 – 509Effector region By similarity

Sites

Binding site451GTP

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue821O-AMP-tyrosine; by Legionella DrrA
Modified residue2081Cysteine methyl ester By similarity
Lipidation2061S-geranylgeranyl cysteine By similarity
Lipidation2081S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis271T → N: Loss of APBA1-binding. Ref.12
Mutagenesis721Q → L: Interacts with APBA1. Ref.12

Secondary structure

.............................. 208
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9NRW1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E81C831996E2446D

FASTA20823,462
        10         20         30         40         50         60 
MSAGGDFGNP LRKFKLVFLG EQSVGKTSLI TRFMYDSFDN TYQATIGIDF LSKTMYLEDR 

        70         80         90        100        110        120 
TVRLQLWDTA GQERFRSLIP SYIRDSTVAV VVYDITNLNS FQQTSKWIDD VRTERGSDVI 

       130        140        150        160        170        180 
IMLVGNKTDL ADKRQITIEE GEQRAKELSV MFIETSAKTG YNVKQLFRRV ASALPGMENV 

       190        200 
QEKSKEGMID IKLDKPQEPP ASEGGCSC 

« Hide

References

« Hide 'large scale' references
[1]"The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus."
Opdam F.J.M., Echard A., Croes H.J.E., van den Hurk J.A.J.M., van de Vorstenbosch R.A., Ginsel L.A., Goud B., Fransen J.A.M.
J. Cell Sci. 113:2725-2735(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[7]"RAB GTPases expressed in human melanoma cells."
Chen D., Guo J., Gahl W.A.
Biochim. Biophys. Acta 1355:1-6(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 72-124.
[8]"A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells."
Wanschers B.F.J.F., van de Vorstenbosch R., Schlager M.A., Splinter D., Akhmanova A., Hoogenraad C.C., Wieringa B., Fransen J.A.
Exp. Cell Res. 313:3408-3420(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BICD1, SUBCELLULAR LOCATION.
[9]"Rab6 family proteins interact with the dynein light chain protein DYNLRB1."
Wanschers B.F.J., van de Vorstenbosch R., Wijers M., Wieringa B., King S.M., Fransen J.
Cell Motil. Cytoskeleton 65:183-196(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYNLRB1, SUBCELLULAR LOCATION.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Modulation of Rab GTPase function by a protein phosphocholine transferase."
Mukherjee S., Liu X., Arasaki K., McDonough J., Galan J.E., Roy C.R.
Nature 477:103-106(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: AMPYLATION AT TYR-82.
[12]"A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6."
Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbusch D., Eggert S., Formstecher E., Muthing J., Dreisewerd K., Kins S., Goud B., Barnekow A.
PLoS ONE 8:E64149-E64149(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH APBA1, MUTAGENESIS OF THR-27 AND GLN-72.
[13]"The structure of human neuronal Rab6B in the active and inactive form."
Garcia-Saez I., Tcherniuk S., Kozielski F.
Acta Crystallogr. D 62:725-733(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 13-174.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF166492 mRNA. Translation: AAF61637.1.
AF498940 mRNA. Translation: AAM21088.1.
BT007263 mRNA. Translation: AAP35927.1.
AK312378 mRNA. Translation: BAG35296.1.
CH471052 Genomic DNA. Translation: EAW79158.1.
CH471052 Genomic DNA. Translation: EAW79159.1.
BC002510 mRNA. Translation: AAH02510.1.
U66623 mRNA. Translation: AAC51198.1.
CCDSCCDS3082.1.
RefSeqNP_057661.3. NM_016577.3.
UniGeneHs.715344.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E9SX-ray1.78A/B/C13-174[»]
2FE4X-ray2.30A13-174[»]
2FFQX-ray1.78A13-174[»]
ProteinModelPortalQ9NRW1.
SMRQ9NRW1. Positions 13-174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119610. 8 interactions.
IntActQ9NRW1. 3 interactions.
STRING9606.ENSP00000285208.

PTM databases

PhosphoSiteQ9NRW1.

Polymorphism databases

DMDM13633595.

Proteomic databases

MaxQBQ9NRW1.
PaxDbQ9NRW1.
PRIDEQ9NRW1.

Protocols and materials databases

DNASU51560.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000285208; ENSP00000285208; ENSG00000154917.
ENST00000543906; ENSP00000437797; ENSG00000154917.
GeneID51560.
KEGGhsa:51560.
UCSCuc003epy.3. human.

Organism-specific databases

CTD51560.
GeneCardsGC03M133543.
HGNCHGNC:14902. RAB6B.
HPACAB017713.
neXtProtNX_Q9NRW1.
PharmGKBPA34147.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidQ9NRW1.
KOK07894.
OMAMESVQEK.
OrthoDBEOG77DJ7M.
PhylomeDBQ9NRW1.
TreeFamTF300803.

Gene expression databases

ArrayExpressQ9NRW1.
BgeeQ9NRW1.
GenevestigatorQ9NRW1.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRAB6B. human.
EvolutionaryTraceQ9NRW1.
GeneWikiRAB6B.
GenomeRNAi51560.
NextBio55358.
PROQ9NRW1.

Entry information

Entry nameRAB6B_HUMAN
AccessionPrimary (citable) accession number: Q9NRW1
Secondary accession number(s): B2R5Z9, D3DND3, Q92929
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM