Q9NRW1 (RAB6B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-6B

Gene names

Name:

RAB6B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. Ref.8
Subunit structure	Interacts with CCDC64/BICDR1; leads to its accumulation in the pericentrosomal region By similarity. Interacts with RAB6KIFL. Interacts (GTP-bound) with BICD1 (via C-terminus); the interaction is direct. Interacts (GDP-bound) with DYNLRB1. Ref.8 Ref.9
Subcellular location	Golgi apparatus membrane; Lipid-anchor. Cytoplasmic vesicle. Note: Colocalizes with BICD1 at vesicular structures that align along microtubules. Ref.8 Ref.9
Tissue specificity	Predominantly expressed in brain.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde vesicle-mediated transport, Golgi to ER Non-traceable author statement Ref.1. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Non-traceable author statement Ref.1. Source: UniProtKB Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 208

208

Ras-related protein Rab-6B

PRO_0000121115

Regions

Nucleotide binding

20 – 27

8

GTP

Nucleotide binding

68 – 72

5

GTP

Nucleotide binding

126 – 129

4

GTP

Motif

42 – 50

9

Effector region By similarity

Sites

Binding site

45

1

GTP

Amino acid modifications

Modified residue

82

1

O-AMP-tyrosine; by Legionella DrrA

Modified residue

208

1

Cysteine methyl ester By similarity

Lipidation

206

1

S-geranylgeranyl cysteine By similarity

Lipidation

208

1

S-geranylgeranyl cysteine By similarity

Secondary structure

1

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208

Helix Strand Turn

Details...

Beta strand

15 – 19

5

Helix

26 – 35

10

Beta strand

47 – 57

11

Beta strand

60 – 69

10

Helix

73 – 78

6

Helix

79 – 84

6

Beta strand

87 – 94

8

Helix

98 – 102

5

Helix

104 – 115

12

Beta strand

118 – 126

9

Helix

131 – 133

3

Helix

138 – 147

10

Beta strand

151 – 154

4

Turn

157 – 159

3

Helix

163 – 174

12

Sequences

	Sequence		Length	Mass (Da)
	Q9NRW1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E81C831996E2446D	FASTA	208	23,462
	10 20 30 40 50 60 MSAGGDFGNP LRKFKLVFLG EQSVGKTSLI TRFMYDSFDN TYQATIGIDF LSKTMYLEDR 70 80 90 100 110 120 TVRLQLWDTA GQERFRSLIP SYIRDSTVAV VVYDITNLNS FQQTSKWIDD VRTERGSDVI 130 140 150 160 170 180 IMLVGNKTDL ADKRQITIEE GEQRAKELSV MFIETSAKTG YNVKQLFRRV ASALPGMENV 190 200 QEKSKEGMID IKLDKPQEPP ASEGGCSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus." Opdam F.J.M., Echard A., Croes H.J.E., van den Hurk J.A.J.M., van de Vorstenbosch R.A., Ginsel L.A., Goud B., Fransen J.A.M. J. Cell Sci. 113:2725-2735(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[7]	"RAB GTPases expressed in human melanoma cells." Chen D., Guo J., Gahl W.A. Biochim. Biophys. Acta 1355:1-6(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 72-124.
[8]	"A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells." Wanschers B.F.J.F., van de Vorstenbosch R., Schlager M.A., Splinter D., Akhmanova A., Hoogenraad C.C., Wieringa B., Fransen J.A. Exp. Cell Res. 313:3408-3420(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BICD1, SUBCELLULAR LOCATION.
[9]	"Rab6 family proteins interact with the dynein light chain protein DYNLRB1." Wanschers B.F.J., van de Vorstenbosch R., Wijers M., Wieringa B., King S.M., Fransen J. Cell Motil. Cytoskeleton 65:183-196(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DYNLRB1, SUBCELLULAR LOCATION.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Modulation of Rab GTPase function by a protein phosphocholine transferase." Mukherjee S., Liu X., Arasaki K., McDonough J., Galan J.E., Roy C.R. Nature 477:103-106(2011) [PubMed] [Europe PMC] [Abstract] Cited for: AMPYLATION AT TYR-82.
[12]	"The structure of human neuronal Rab6B in the active and inactive form." Garcia-Saez I., Tcherniuk S., Kozielski F. Acta Crystallogr. D 62:725-733(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 13-174.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF166492 mRNA. Translation: AAF61637.1.
AF498940 mRNA. Translation: AAM21088.1.
BT007263 mRNA. Translation: AAP35927.1.
AK312378 mRNA. Translation: BAG35296.1.
CH471052 Genomic DNA. Translation: EAW79158.1.
CH471052 Genomic DNA. Translation: EAW79159.1.
BC002510 mRNA. Translation: AAH02510.1.
U66623 mRNA. Translation: AAC51198.1.

IPI

IPI00016891.

RefSeq

NP_057661.3. NM_016577.3.

UniGene

Hs.743968.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E9S	X-ray	1.78	A/B/C	13-174	[»]
2FE4	X-ray	2.30	A	13-174	[»]
2FFQ	X-ray	1.78	A	13-174	[»]

ProteinModelPortal

Q9NRW1.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NRW1. 1 interaction.

STRING

9606.ENSP00000285208.

PTM databases

PhosphoSite

Q9NRW1.

Polymorphism databases

DMDM

13633595.

Proteomic databases

PaxDb

Q9NRW1.

PRIDE

Q9NRW1.

Protocols and materials databases

DNASU

51560.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000285208; ENSP00000285208; ENSG00000154917.
ENST00000543906; ENSP00000437797; ENSG00000154917.

GeneID

51560.

KEGG

hsa:51560.

UCSC

uc003epy.3. human.

Organism-specific databases

CTD

51560.

GeneCards

GC03M133543.

HGNC

HGNC:14902. RAB6B.

HPA

CAB017713.

neXtProt

NX_Q9NRW1.

PharmGKB

PA34147.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

InParanoid

Q9NRW1.

KO

K07894.

OMA

ETSKEGM.

OrthoDB

EOG4NP74D.

PhylomeDB

Q9NRW1.

Gene expression databases

ArrayExpress

Q9NRW1.

Bgee

Q9NRW1.

Genevestigator

Q9NRW1.

GermOnline

ENSG00000154917. Homo sapiens.

Family and domain databases

InterPro

IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

SUPFAM

SSF52540. SSF52540. 1 hit.

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

RAB6B. human.

EvolutionaryTrace

Q9NRW1.

GenomeRNAi

51560.

NextBio

55358.

Entry information

Entry name

RAB6B_HUMAN

Accession

Primary (citable) accession number: Q9NRW1
Secondary accession number(s): B2R5Z9, D3DND3, Q92929

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.