Reviewed, UniProtKB/Swiss-Prot Q9NRR6 (INP5E_HUMAN)

Names and origin

Protein names

Recommended name:
    72 kDa inositol polyphosphate 5-phosphatase
    EC=3.1.3.36
Alternative name(s):
    Phosphatidylinositol-4,5-bisphosphate 5-phosphatase
    Phosphatidylinositol polyphosphate 5-phosphatase type IV

Gene names

Name:

INPP5E

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts phosphatidylinositol-3,4,5-triphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. Specific for lipid substrates, inactive towards water soluble inositol phosphates. Ref.1
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Subcellular location	Golgi apparatus › Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Peripheral membrane protein associated with Golgi stacks By similarity.
Tissue specificity	Detected in brain, heart, pancreas, testis and spleen. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7
Miscellaneous	Active in the presence of octyl-glucoside or Triton X-100, but completely inhibited by CTAB.
Sequence similarities	Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type IV family.
Sequence caution	The sequence AAB03215.1 differs from that shown. Reason: Miscellaneous discrepancy. Several sequencing problems.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inositol-polyphosphate 5-phosphatase activity Ref.1 Traceable author statement. Source: UniProtKB phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NRR6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NRR6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     346-379: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	72 kDa inositol polyphosphate 5-phosphatase		PRO_0000209747
Regions
	Repeat	10 – 13	4	1
	Repeat	15 – 18	4	2
	Repeat	28 – 31	4	3
	Repeat	39 – 42	4	4
	Repeat	55 – 58	4	5
	Repeat	69 – 71	3	6
	Repeat	72 – 74	3	7
	Repeat	75 – 78	4	8
	Repeat	121 – 124	4	9
	Repeat	169 – 172	4	10
	Repeat	183 – 185	3	11
	Repeat	190 – 193	4	12
	Repeat	236 – 239	4	13
	Region	10 – 242	233	13 X 4 AA repeats of P-X-X-P
	Compositional bias	92 – 97	6	Poly-Arg
Amino acid modifications
	Modified residue	99	1	Phosphoserine Ref.7
	Modified residue	109	1	Phosphothreonine Ref.6
	Modified residue	241	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	346 – 379	34	Missing in isoform 2.		VSP_009799
	Natural variant	201	1	I → M: dbSNP rs36064831.		VAR_047078
Experimental info
	Sequence conflict	345	1	R → T in AAF81404. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 4, 2008. Version 2. Checksum: 5B6CFB75CD0ADC9A	FASTA	644	70,205
	10 20 30 40 50 60 MPSKAENLRP SEPAPQPPEG RTLQGQLPGA PPAQRAGSPP DAPGSESPAL ACSTPATPSG 70 80 90 100 110 120 EDPPARAAPI APRPPARPRL ERALSLDDKG WRRRRFRGSQ EDLEARNGTS PSRGSVQSEG 130 140 150 160 170 180 PGAPAHSCSP PCLSTSLQEI PKSRGVLSSE RGSPSSGGNP LSGVASSSPN LPHRDAAVAG 190 200 210 220 230 240 SSPRLPSLLP PRPPPALSLD IASDSLRTAN KVDSDLADYK LRAQPLLVRA HSSLGPGRPR 250 260 270 280 290 300 SPLACDDCSL RSAKSSFSLL APIRSKDVRS RSYLEGSLLA SGALLGADEL ARYFPDRNVA 310 320 330 340 350 360 LFVATWNMQG QKELPPSLDE FLLPAEADYA QDLYVIGVQE GCSDRREWET RLQETLGPHY 370 380 390 400 410 420 VLLSSAAHGV LYMSLFIRRD LIWFCSEVEC STVTTRIVSQ IKTKGALGIS FTFFGTSFLF 430 440 450 460 470 480 ITSHFTSGDG KVAERLLDYT RTVQALVLPR NVPDTNPYRS SAADVTTRFD EVFWFGDFNF 490 500 510 520 530 540 RLSGGRTVVD ALLCQGLVVD VPALLQHDQL IREMRKGSIF KGFQEPDIHF LPSYKFDIGK 550 560 570 580 590 600 DTYDSTSKQR TPSYTDRVLY RSRHKGDICP VSYSSCPGIK TSDHRPVYGL FRVKVRPGRD 610 620 630 640 NIPLAAGKFD RELYLLGIKR RISKEIQRQQ ALQSQNSSTI CSVS
	Isoform 2. Checksum: 093CCDE3EBB9DD9A	FASTA	610	66,193
	10 20 30 40 50 60 MPSKAENLRP SEPAPQPPEG RTLQGQLPGA PPAQRAGSPP DAPGSESPAL ACSTPATPSG 70 80 90 100 110 120 EDPPARAAPI APRPPARPRL ERALSLDDKG WRRRRFRGSQ EDLEARNGTS PSRGSVQSEG 130 140 150 160 170 180 PGAPAHSCSP PCLSTSLQEI PKSRGVLSSE RGSPSSGGNP LSGVASSSPN LPHRDAAVAG 190 200 210 220 230 240 SSPRLPSLLP PRPPPALSLD IASDSLRTAN KVDSDLADYK LRAQPLLVRA HSSLGPGRPR 250 260 270 280 290 300 SPLACDDCSL RSAKSSFSLL APIRSKDVRS RSYLEGSLLA SGALLGADEL ARYFPDRNVA 310 320 330 340 350 360 LFVATWNMQG QKELPPSLDE FLLPAEADYA QDLYVIGVQE GCSDRDLIWF CSEVECSTVT 370 380 390 400 410 420 TRIVSQIKTK GALGISFTFF GTSFLFITSH FTSGDGKVAE RLLDYTRTVQ ALVLPRNVPD 430 440 450 460 470 480 TNPYRSSAAD VTTRFDEVFW FGDFNFRLSG GRTVVDALLC QGLVVDVPAL LQHDQLIREM 490 500 510 520 530 540 RKGSIFKGFQ EPDIHFLPSY KFDIGKDTYD STSKQRTPSY TDRVLYRSRH KGDICPVSYS 550 560 570 580 590 600 SCPGIKTSDH RPVYGLFRVK VRPGRDNIPL AAGKFDRELY LLGIKRRISK EIQRQQALQS 610 QNSSTICSVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation and characterization of a cDNA encoding phospholipid-specific inositol polyphosphate 5-phosphatase." Kisseleva M.V., Wilson M.P., Majerus P.W. J. Biol. Chem. 275:20110-20116(2000) [PubMed: 10764818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	Nussbaum R.L. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 469-644 (ISOFORM 1).
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-109, MASS SPECTROMETRY.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF187891 mRNA. Translation: AAF81404.1. AL592301 Genomic DNA. Translation: CAI13947.1. CH471090 Genomic DNA. Translation: EAW88234.1. BC028032 mRNA. Translation: AAH28032.1. U45974 mRNA. Translation: AAB03215.1. Sequence problems.
IPI	IPI00181799. IPI00409719.
RefSeq	NP_063945.2.
UniGene	Hs.120998
3D structure databases
HSSP	HSSP built from PDB template 1I9Z based on UniProtKB O43001.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NRR6.
PTM databases
PhosphoSite	Q9NRR6.
Proteomic databases
PRIDE	Q9NRR6.
Genome annotation databases
Ensembl	ENST00000371712; ENSP00000360777; ENSG00000148384; Homo sapiens. [Genome view] ENST00000445731; ENSP00000402715; ENSG00000148384; Homo sapiens. [Genome view]
GeneID	56623.
KEGG	hsa:56623.
UCSC	uc004cho.1. human.
Organism-specific databases
CTD	56623.
GeneCards	GC09M138442.
HGNC	HGNC:21474. INPP5E.
PharmGKB	PA134890388.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NRR6.
HOVERGEN	Q9NRR6.
OMA	SGMISTS.
Enzyme and pathway databases
BRENDA	3.1.3.36. 247.
Gene expression databases
ArrayExpress	Q9NRR6.
Bgee	Q9NRR6.
CleanEx	HS_INPP5E.
Genevestigator	Q9NRR6.
GermOnline	ENSG00000148384. Homo sapiens.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INP5E_HUMAN

Accession

Primary (citable) accession number: Q9NRR6
Secondary accession number(s): Q15734, Q6PIV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	November 4, 2008
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.