Q9NRR3 (C42S2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CDC42 small effector protein 2

Alternative name(s):
Small effector of CDC42 protein 2

Gene names

Name:	CDC42SE2
Synonyms:	SPEC2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the organization of the actin cytoskeleton by acting downstream of CDC42, inducing actin filament assembly. Alters CDC42-induced cell shape changes. In activated T-cells, may play a role in CDC42-mediated F-actin accumulation at the immunological synapse. May play a role in early contractile events in phagocytosis in macrophages. Ref.1 Ref.5
Subunit structure	Interacts with CDC42 (in GTP-bound form). Interacts weakly with RAC1 and not at all with RHOA. Ref.1
Subcellular location	Cytoplasm › cytoskeleton. Cell membrane; Lipid-anchor. Cell projection › phagocytic cup. Note: Recruited to the activated TCR prior actin polymerization. Localizes at the phagocytic cup of macrophages. Ref.1 Ref.5 Ref.6
Tissue specificity	Widely expressed. Expressed at higher level in T-lymphocytes. Highly expressed in CCRF-CEM T-lymphocytes, Jurkat T-lymphocytes, and Raji B-lymphocytes compared (at protein level). Ref.5
Domain	The CRIB domain mediates interaction with CDC42.
Miscellaneous	CDC42SE2 is mapped in the genomic region associated with schizophrenia.
Sequence similarities	Belongs to the CDC42SE/SPEC family. Contains 1 CRIB domain.

Ontologies

Keywords
Biological process	Cell shape Phagocytosis
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phagocytosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of signal transduction Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	cell projection Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA phagocytic cup Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	structural molecule activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 84	84	CDC42 small effector protein 2		PRO_0000334639
Regions
	Domain	29 – 42	14	CRIB
	Compositional bias	20 – 23	4	Poly-Arg
Amino acid modifications
	Lipidation	10	1	S-palmitoyl cysteine Ref.5
	Lipidation	11	1	S-palmitoyl cysteine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9NRR3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 98C5E82176DA990A	FASTA	84	9,223
	10 20 30 40 50 60 MSEFWLCFNC CIAEQPQPKR RRRIDRSMIG EPTNFVHTAH VGSGDLFSGM NSVSSIQNQM 70 80 QSKGGYGGGM PANVQMQLVD TKAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SPECs, small binding proteins for Cdc42." Pirone D.M., Fukuhara S., Gutkind J.S., Burbelo P.D. J. Biol. Chem. 275:22650-22656(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC42.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The role of SPECs, small Cdc42-binding proteins, in F-actin accumulation at the immunological synapse." Ching K.H., Kisailus A.E., Burbelo P.D. J. Biol. Chem. 280:23660-23667(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PALMITOYLATION AT CYS-10 AND CYS-11.
[6]	"Biochemical characterization of distinct regions of SPEC molecules and their role in phagocytosis." Ching K.H., Kisailus A.E., Burbelo P.D. Exp. Cell Res. 313:10-21(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Haplotypes spanning SPEC2, PDZ-GEF2 and ACSL6 genes are associated with schizophrenia." Chen X., Wang X., Hossain S., O'Neill F.A., Walsh D., Pless L., Chowdari K.V., Nimgaonkar V.L., Schwab S.G., Wildenauer D.B., Sullivan P.F., van den Oord E., Kendler K.S. Hum. Mol. Genet. 15:3329-3342(2006) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE SUSCEPTIBILITY TO SCHIZOPHRENIA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF189692 mRNA. Translation: AAF87598.1. AK312406 mRNA. Translation: BAG35319.1. CH471062 Genomic DNA. Translation: EAW62373.1. BC096703 mRNA. Translation: AAH96703.1. BC096738 mRNA. Translation: AAH96738.1. BC098349 mRNA. Translation: AAH98349.1.
IPI	IPI00024973.
RefSeq	NP_001033791.1. NM_001038702.1. NP_064625.1. NM_020240.2.
UniGene	Hs.508829.
3D structure databases
ProteinModelPortal	Q9NRR3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000353706.
PTM databases
PhosphoSite	Q9NRR3.
Polymorphism databases
DMDM	74719133.
Proteomic databases
PaxDb	Q9NRR3.
PRIDE	Q9NRR3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360515; ENSP00000353706; ENSG00000158985. ENST00000395246; ENSP00000378667; ENSG00000158985. ENST00000505065; ENSP00000427421; ENSG00000158985.
GeneID	56990.
KEGG	hsa:56990.
UCSC	uc003kvh.3. human.
Organism-specific databases
CTD	56990.
GeneCards	GC05P130581.
H-InvDB	HIX0005148.
HGNC	HGNC:18547. CDC42SE2.
HPA	HPA038624.
neXtProt	NX_Q9NRR3.
PharmGKB	PA133787056.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG78697.
HOGENOM	HOG000006512.
HOVERGEN	HBG107546.
InParanoid	Q9NRR3.
OMA	ISANVQM.
OrthoDB	EOG4G7C10.
PhylomeDB	Q9NRR3.
Gene expression databases
ArrayExpress	Q9NRR3.
Bgee	Q9NRR3.
CleanEx	HS_CDC42SE2.
Genevestigator	Q9NRR3.
Family and domain databases
InterPro	IPR000095. PAK_box_Rho-bd. [Graphical view]
Pfam	PF00786. PBD. 1 hit. [Graphical view]
PROSITE	PS50108. CRIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	56990.
NextBio	62683.

Entry information

Entry name

C42S2_HUMAN

Accession

Primary (citable) accession number: Q9NRR3
Secondary accession number(s): B2R622, Q4KMT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.