Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phospholipid scramblase 4

Gene

PLSCR4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.

Cofactori

Ca2+1 Publication, Mg2+1 Publication

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • CD4 receptor binding Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • phospholipid scramblase activity Source: UniProtKB

GO - Biological processi

  • cellular response to lipopolysaccharide Source: Ensembl
  • phospholipid scrambling Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114698-MONOMER.
BRENDAi3.6.99.B1. 2681.

Protein family/group databases

TCDBi9.A.36.1.4. the ca(2+)-dependent phospholipid scramblase (scramblase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid scramblase 4
Short name:
PL scramblase 4
Alternative name(s):
Ca(2+)-dependent phospholipid scramblase 4
Cell growth-inhibiting gene 43 protein
TRA1
Gene namesi
Name:PLSCR4
ORF Names:GIG43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:16497. PLSCR4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 303CytoplasmicBy similarityAdd BLAST303
Transmembranei304 – 320HelicalSequence analysisAdd BLAST17
Topological domaini321 – 329ExtracellularBy similarity9

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi290D → A: 50% decrease in scramblase activity in presence of Ca2+, and 40% decrease in scramblase activity in presence of Mg2+. 1 Publication1

Organism-specific databases

DisGeNETi57088.
OpenTargetsiENSG00000114698.
PharmGKBiPA33422.

Polymorphism and mutation databases

BioMutaiPLSCR4.
DMDMi212276457.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007921 – 329Phospholipid scramblase 4Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83Phosphotyrosine; by ABLBy similarity1
Modified residuei88Phosphotyrosine; by ABLBy similarity1
Lipidationi250S-palmitoyl cysteineCurated1
Lipidationi255S-palmitoyl cysteineCurated1

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9NRQ2.
PaxDbiQ9NRQ2.
PeptideAtlasiQ9NRQ2.
PRIDEiQ9NRQ2.

PTM databases

iPTMnetiQ9NRQ2.
PhosphoSitePlusiQ9NRQ2.
SwissPalmiQ9NRQ2.

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, uterus, small intestine and colon. Not detected in peripheral blood lymphocytes.

Gene expression databases

BgeeiENSG00000114698.
CleanExiHS_PLSCR4.
ExpressionAtlasiQ9NRQ2. baseline and differential.
GenevisibleiQ9NRQ2. HS.

Organism-specific databases

HPAiHPA002276.

Interactioni

Subunit structurei

Interacts with PDCD6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Hoxa1P090224EBI-769257,EBI-3957603From a different organism.
MTUS2Q5JR594EBI-769257,EBI-742948
NOTCH2NLQ7Z3S93EBI-769257,EBI-945833
TRIP13Q156453EBI-769257,EBI-358993

GO - Molecular functioni

  • CD4 receptor binding Source: UniProtKB
  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

BioGridi121357. 16 interactors.
IntActiQ9NRQ2. 20 interactors.
MINTiMINT-1443866.
STRINGi9606.ENSP00000347038.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q5UX-ray2.50B271-283[»]
ProteinModelPortaliQ9NRQ2.
SMRiQ9NRQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 98Proline-rich domain (PRD)By similarityAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi18 – 25SH3-binding 1Sequence analysis8
Motifi30 – 33WW-bindingSequence analysis4
Motifi41 – 49SH3-binding 2Sequence analysis9
Motifi98 – 106SH3-binding 3Sequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 202Cys-rich8

Domaini

The N-terminal proline-rich domain (PRD) is required for phospholipid scramblase activity.By similarity

Sequence similaritiesi

Belongs to the phospholipid scramblase family.Curated

Keywords - Domaini

Repeat, SH3-binding, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0621. Eukaryota.
ENOG410XSYS. LUCA.
GeneTreeiENSGT00390000002884.
HOGENOMiHOG000237356.
HOVERGENiHBG019157.
InParanoidiQ9NRQ2.
OMAiNRYDIKN.
OrthoDBiEOG091G0CUL.
PhylomeDBiQ9NRQ2.
TreeFamiTF314939.

Family and domain databases

InterProiIPR005552. Scramblase.
[Graphical view]
PANTHERiPTHR23248. PTHR23248. 1 hit.
PfamiPF03803. Scramblase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NRQ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGVVPTAPE QPAGEMENQT KPPDPRPDAP PEYNSHFLPG PPGTAVPPPT
60 70 80 90 100
GYPGGLPMGY YSPQQPSTFP LYQPVGGIHP VRYQPGKYPM PNQSVPITWM
110 120 130 140 150
PGPTPMANCP PGLEYLVQLD NIHVLQHFEP LEMMTCFETN NRYDIKNNSD
160 170 180 190 200
QMVYIVTEDT DDFTRNAYRT LRPFVLRVTD CMGREIMTMQ RPFRCTCCCF
210 220 230 240 250
CCPSARQELE VQCPPGVTIG FVAEHWNLCR AVYSIQNEKK ENVMRVRGPC
260 270 280 290 300
STYGCGSDSV FEVKSLDGIS NIGSIIRKWN GLLSAMADAD HFDIHFPLDL
310 320
DVKMKAMIFG ACFLIDFMYF ERSPPQRSR
Length:329
Mass (Da):37,005
Last modified:November 4, 2008 - v2
Checksum:i12BE86728D54F794
GO
Isoform 2 (identifier: Q9NRQ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.
     119-208: Missing.

Note: No experimental confirmation available.
Show »
Length:224
Mass (Da):24,797
Checksum:i55E36F0305DAB876
GO

Sequence cautioni

The sequence AAP97186 differs from that shown. Reason: Frameshift at positions 152, 155, 163 and 325.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 57LP → FL in AAP97186 (Ref. 8) Curated2
Sequence conflicti74P → S in AAT52217 (Ref. 2) Curated1
Sequence conflicti149S → L in AAP97186 (Ref. 8) Curated1
Sequence conflicti163F → V in AAP97186 (Ref. 8) Curated1
Sequence conflicti175V → G in AAP97186 (Ref. 8) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01131534N → S.2 PublicationsCorresponds to variant rs3762685dbSNPEnsembl.1
Natural variantiVAR_011316155I → V.2 PublicationsCorresponds to variant rs1061409dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0429311 – 15Missing in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_042932119 – 208Missing in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199023 mRNA. Translation: AAF89960.1.
AY550971 mRNA. Translation: AAT52217.1.
AK128442 mRNA. Translation: BAC87442.1.
AK290214 mRNA. Translation: BAF82903.1.
AL833760 mRNA. Translation: CAH56232.1.
AC092982 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78935.1.
BC028354 mRNA. Translation: AAH28354.1.
AF087887 mRNA. Translation: AAP97186.1. Sequence problems.
CCDSiCCDS3133.1. [Q9NRQ2-1]
CCDS54651.1. [Q9NRQ2-2]
RefSeqiNP_001121776.1. NM_001128304.1. [Q9NRQ2-1]
NP_001121777.1. NM_001128305.1. [Q9NRQ2-1]
NP_001121778.1. NM_001128306.1.
NP_001170775.1. NM_001177304.1. [Q9NRQ2-2]
NP_065086.2. NM_020353.2. [Q9NRQ2-1]
XP_005247711.1. XM_005247654.2. [Q9NRQ2-1]
XP_005247712.1. XM_005247655.2. [Q9NRQ2-1]
XP_011511333.1. XM_011513031.2. [Q9NRQ2-1]
UniGeneiHs.477869.

Genome annotation databases

EnsembliENST00000354952; ENSP00000347038; ENSG00000114698. [Q9NRQ2-1]
ENST00000433593; ENSP00000415605; ENSG00000114698. [Q9NRQ2-2]
ENST00000446574; ENSP00000399315; ENSG00000114698. [Q9NRQ2-1]
ENST00000493382; ENSP00000419040; ENSG00000114698. [Q9NRQ2-1]
GeneIDi57088.
KEGGihsa:57088.
UCSCiuc003evt.6. human. [Q9NRQ2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199023 mRNA. Translation: AAF89960.1.
AY550971 mRNA. Translation: AAT52217.1.
AK128442 mRNA. Translation: BAC87442.1.
AK290214 mRNA. Translation: BAF82903.1.
AL833760 mRNA. Translation: CAH56232.1.
AC092982 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78935.1.
BC028354 mRNA. Translation: AAH28354.1.
AF087887 mRNA. Translation: AAP97186.1. Sequence problems.
CCDSiCCDS3133.1. [Q9NRQ2-1]
CCDS54651.1. [Q9NRQ2-2]
RefSeqiNP_001121776.1. NM_001128304.1. [Q9NRQ2-1]
NP_001121777.1. NM_001128305.1. [Q9NRQ2-1]
NP_001121778.1. NM_001128306.1.
NP_001170775.1. NM_001177304.1. [Q9NRQ2-2]
NP_065086.2. NM_020353.2. [Q9NRQ2-1]
XP_005247711.1. XM_005247654.2. [Q9NRQ2-1]
XP_005247712.1. XM_005247655.2. [Q9NRQ2-1]
XP_011511333.1. XM_011513031.2. [Q9NRQ2-1]
UniGeneiHs.477869.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q5UX-ray2.50B271-283[»]
ProteinModelPortaliQ9NRQ2.
SMRiQ9NRQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121357. 16 interactors.
IntActiQ9NRQ2. 20 interactors.
MINTiMINT-1443866.
STRINGi9606.ENSP00000347038.

Protein family/group databases

TCDBi9.A.36.1.4. the ca(2+)-dependent phospholipid scramblase (scramblase) family.

PTM databases

iPTMnetiQ9NRQ2.
PhosphoSitePlusiQ9NRQ2.
SwissPalmiQ9NRQ2.

Polymorphism and mutation databases

BioMutaiPLSCR4.
DMDMi212276457.

Proteomic databases

MaxQBiQ9NRQ2.
PaxDbiQ9NRQ2.
PeptideAtlasiQ9NRQ2.
PRIDEiQ9NRQ2.

Protocols and materials databases

DNASUi57088.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354952; ENSP00000347038; ENSG00000114698. [Q9NRQ2-1]
ENST00000433593; ENSP00000415605; ENSG00000114698. [Q9NRQ2-2]
ENST00000446574; ENSP00000399315; ENSG00000114698. [Q9NRQ2-1]
ENST00000493382; ENSP00000419040; ENSG00000114698. [Q9NRQ2-1]
GeneIDi57088.
KEGGihsa:57088.
UCSCiuc003evt.6. human. [Q9NRQ2-1]

Organism-specific databases

CTDi57088.
DisGeNETi57088.
GeneCardsiPLSCR4.
HGNCiHGNC:16497. PLSCR4.
HPAiHPA002276.
MIMi607612. gene.
neXtProtiNX_Q9NRQ2.
OpenTargetsiENSG00000114698.
PharmGKBiPA33422.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0621. Eukaryota.
ENOG410XSYS. LUCA.
GeneTreeiENSGT00390000002884.
HOGENOMiHOG000237356.
HOVERGENiHBG019157.
InParanoidiQ9NRQ2.
OMAiNRYDIKN.
OrthoDBiEOG091G0CUL.
PhylomeDBiQ9NRQ2.
TreeFamiTF314939.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114698-MONOMER.
BRENDAi3.6.99.B1. 2681.

Miscellaneous databases

GeneWikiiPLSCR4.
GenomeRNAii57088.
PROiQ9NRQ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114698.
CleanExiHS_PLSCR4.
ExpressionAtlasiQ9NRQ2. baseline and differential.
GenevisibleiQ9NRQ2. HS.

Family and domain databases

InterProiIPR005552. Scramblase.
[Graphical view]
PANTHERiPTHR23248. PTHR23248. 1 hit.
PfamiPF03803. Scramblase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLS4_HUMAN
AccessioniPrimary (citable) accession number: Q9NRQ2
Secondary accession number(s): A8K2E9
, Q2TTR3, Q658L3, Q6ZR73, Q7Z505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 4, 2008
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.