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Q9NRQ2 (PLS4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipid scramblase 4

Short name=PL scramblase 4
Alternative name(s):
Ca(2+)-dependent phospholipid scramblase 4
Cell growth-inhibiting gene 43 protein
TRA1
Gene names
Name:PLSCR4
ORF Names:GIG43
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.

Cofactor

Calcium or magnesium. Ref.10

Subunit structure

Interacts with PDCD6. Ref.9

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed in heart, brain, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, uterus, small intestine and colon. Not detected in peripheral blood lymphocytes.

Sequence similarities

Belongs to the phospholipid scramblase family.

Sequence caution

The sequence AAP97186.1 differs from that shown. Reason: Frameshift at positions 152, 155, 163 and 325.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Hoxa1P090224EBI-769257,EBI-3957603From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NRQ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NRQ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.
     119-208: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Phospholipid scramblase 4
PRO_0000100792

Regions

Topological domain1 – 303303Cytoplasmic By similarity
Transmembrane304 – 32017Helical; Potential
Topological domain321 – 3299Extracellular By similarity
Motif18 – 258SH3-binding 1 Potential
Motif30 – 334WW-binding Potential
Motif41 – 499SH3-binding 2 Potential
Motif98 – 1069SH3-binding 3 Potential
Compositional bias195 – 2028Cys-rich

Amino acid modifications

Modified residue831Phosphotyrosine; by ABL By similarity
Modified residue881Phosphotyrosine; by ABL By similarity
Lipidation2501S-palmitoyl cysteine Probable
Lipidation2551S-palmitoyl cysteine Probable

Natural variations

Alternative sequence1 – 1515Missing in isoform 2.
VSP_042931
Alternative sequence119 – 20890Missing in isoform 2.
VSP_042932
Natural variant341N → S. Ref.1 Ref.2
Corresponds to variant rs3762685 [ dbSNP | Ensembl ].
VAR_011315
Natural variant1551I → V. Ref.1 Ref.2
Corresponds to variant rs1061409 [ dbSNP | Ensembl ].
VAR_011316

Experimental info

Mutagenesis2901D → A: 50% decrease in scramblase activity in presence of Ca2+, and 40% decrease in scramblase activity in presence of Mg2+. Ref.10
Sequence conflict56 – 572LP → FL in AAP97186. Ref.8
Sequence conflict741P → S in AAT52217. Ref.2
Sequence conflict1491S → L in AAP97186. Ref.8
Sequence conflict1631F → V in AAP97186. Ref.8
Sequence conflict1751V → G in AAP97186. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 4, 2008. Version 2.
Checksum: 12BE86728D54F794

FASTA32937,005
        10         20         30         40         50         60 
MSGVVPTAPE QPAGEMENQT KPPDPRPDAP PEYNSHFLPG PPGTAVPPPT GYPGGLPMGY 

        70         80         90        100        110        120 
YSPQQPSTFP LYQPVGGIHP VRYQPGKYPM PNQSVPITWM PGPTPMANCP PGLEYLVQLD 

       130        140        150        160        170        180 
NIHVLQHFEP LEMMTCFETN NRYDIKNNSD QMVYIVTEDT DDFTRNAYRT LRPFVLRVTD 

       190        200        210        220        230        240 
CMGREIMTMQ RPFRCTCCCF CCPSARQELE VQCPPGVTIG FVAEHWNLCR AVYSIQNEKK 

       250        260        270        280        290        300 
ENVMRVRGPC STYGCGSDSV FEVKSLDGIS NIGSIIRKWN GLLSAMADAD HFDIHFPLDL 

       310        320 
DVKMKAMIFG ACFLIDFMYF ERSPPQRSR 

« Hide

Isoform 2 [UniParc].

Checksum: 55E36F0305DAB876
Show »

FASTA22424,797

References

« Hide 'large scale' references
[1]"Identification of three new members of the phospholipid scramblase gene family."
Wiedmer T., Zhou Q., Kwoh D.Y., Sims P.J.
Biochim. Biophys. Acta 1467:244-253(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-34 AND VAL-155.
Tissue: Pancreas.
[2]"Identification of a human cell growth inhibiting gene."
Kim J.W.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-34 AND VAL-155.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Thalamus and Thymus.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Stomach.
[5]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[8]"Cloning and characterization of a novel human cDNA homology to murine TRA1 mRNA."
Cui W.C., Yu L., Gao J., Fan Y.X., Xu Y.F., Zhao S.Y.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 46-329 (ISOFORM 1).
[9]"Identification of Alix-type and non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants."
Shibata H., Suzuki H., Kakiuchi T., Inuzuka T., Yoshida H., Mizuno T., Maki M.
J. Biol. Chem. 283:9623-9632(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PDCD6.
[10]"Biochemical and functional characterization of human phospholipid scramblase 4 (hPLSCR4)."
Francis V.G., Gummadi S.N.
Biol. Chem. 393:1173-1181(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: COFACTOR, MUTAGENESIS OF ASP-290.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF199023 mRNA. Translation: AAF89960.1.
AY550971 mRNA. Translation: AAT52217.1.
AK128442 mRNA. Translation: BAC87442.1.
AK290214 mRNA. Translation: BAF82903.1.
AL833760 mRNA. Translation: CAH56232.1.
AC092982 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78935.1.
BC028354 mRNA. Translation: AAH28354.1.
AF087887 mRNA. Translation: AAP97186.1. Sequence problems.
RefSeqNP_001121776.1. NM_001128304.1.
NP_001121777.1. NM_001128305.1.
NP_001121778.1. NM_001128306.1.
NP_001170775.1. NM_001177304.1.
NP_065086.2. NM_020353.2.
XP_005247711.1. XM_005247654.1.
XP_005247712.1. XM_005247655.1.
UniGeneHs.477869.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3Q5UX-ray2.50B271-283[»]
ProteinModelPortalQ9NRQ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121357. 7 interactions.
IntActQ9NRQ2. 16 interactions.
MINTMINT-1443866.
STRING9606.ENSP00000347038.

PTM databases

PhosphoSiteQ9NRQ2.

Polymorphism databases

DMDM212276457.

Proteomic databases

PaxDbQ9NRQ2.
PRIDEQ9NRQ2.

Protocols and materials databases

DNASU57088.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354952; ENSP00000347038; ENSG00000114698. [Q9NRQ2-1]
ENST00000433593; ENSP00000415605; ENSG00000114698. [Q9NRQ2-2]
ENST00000446574; ENSP00000399315; ENSG00000114698. [Q9NRQ2-1]
ENST00000493382; ENSP00000419040; ENSG00000114698. [Q9NRQ2-1]
GeneID57088.
KEGGhsa:57088.
UCSCuc003evt.4. human. [Q9NRQ2-1]
uc003evu.4. human. [Q9NRQ2-2]

Organism-specific databases

CTD57088.
GeneCardsGC03M145910.
HGNCHGNC:16497. PLSCR4.
HPAHPA002276.
MIM607612. gene.
neXtProtNX_Q9NRQ2.
PharmGKBPA33422.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG317744.
HOGENOMHOG000237356.
HOVERGENHBG019157.
InParanoidQ9NRQ2.
OMANRYDIKN.
OrthoDBEOG77T14X.
PhylomeDBQ9NRQ2.
TreeFamTF314939.

Gene expression databases

ArrayExpressQ9NRQ2.
BgeeQ9NRQ2.
CleanExHS_PLSCR4.
GenevestigatorQ9NRQ2.

Family and domain databases

InterProIPR005552. Scramblase.
[Graphical view]
PANTHERPTHR23248. PTHR23248. 1 hit.
PfamPF03803. Scramblase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPLSCR4.
GenomeRNAi57088.
NextBio62887.
PROQ9NRQ2.
SOURCESearch...

Entry information

Entry namePLS4_HUMAN
AccessionPrimary (citable) accession number: Q9NRQ2
Secondary accession number(s): A8K2E9 expand/collapse secondary AC list , Q2TTR3, Q658L3, Q6ZR73, Q7Z505
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 4, 2008
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM