Reviewed, UniProtKB/Swiss-Prot Q9NRP7 (STK36_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase 36
    EC=2.7.11.1
Alternative name(s):
    Fused homolog

Gene names

Name:	STK36
Synonyms:	KIAA1278

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine/threonine protein kinase required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB P23647
Cofactor	Magnesium.
Subcellular location	Cytoplasm. Nucleus. Note: Low levels also present in the nucleus. Ref.1
Tissue specificity	Expressed at low levels in most fetal tissues, adult ovaries and at high levels in adult testis, where it is localized in germ cells. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence BAB14184.1 differs from that shown. Reason: Frameshift at position 987.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	post-embryonic development Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid phosphorylation Ref.1 Traceable author statement. Source: UniProtKB regulation of transcription factor activity Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Ref.1 Traceable author statement. Source: UniProtKB magnesium ion binding Ref.1 Inferred by curator. Source: UniProtKB protein serine/threonine kinase activity Ref.1 Traceable author statement. Source: UniProtKB transcription factor binding Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARMC6	Q6NXE6	1	EBI-863797,EBI-1053802
GLI1	P08151	1	EBI-863797,EBI-308084
Gli2	Q8K0K3	1	EBI-863797,EBI-308039	From a different organism.
GLI3	P10071	1	EBI-863797,EBI-308055
SUFU	Q9UMX1	1	EBI-863797,EBI-740595

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.3 Ref.5 Ref.4 (identifier: Q9NRP7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q9NRP7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     838-858: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1315	1315	Serine/threonine-protein kinase 36		PRO_0000229020
Regions
	Domain	4 – 254	251	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity UniProtKB P23647
Sites
	Active site	125	1	Proton acceptor By similarity UniProtKB P23647
	Binding site	33	1	ATP UniProtKB P23647
Natural variations
	Alternative sequence	838 – 858	21	Missing in isoform 2. Ref.2		VSP_051983
	Natural variant	90	1	I → M Ref.6		VAR_041177
	Natural variant	240	1	R → W Ref.6		VAR_041178
	Natural variant	295	1	K → R Ref.6		VAR_041179
	Natural variant	329	1	D → N Ref.6		VAR_041180
	Natural variant	462	1	L → V Ref.6		VAR_041181
	Natural variant	463	1	K → N: dbSNP rs17856747. Ref.3		VAR_025727
	Natural variant	476	1	F → S Ref.6		VAR_041182
	Natural variant	477	1	R → W Ref.6		VAR_041183
	Natural variant	583	1	R → Q Ref.6		VAR_041184
	Natural variant	638	1	Q → P: dbSNP rs6709303.		VAR_057112
	Natural variant	660	1	S → C in a breast pleomorphic lobular carcinoma sample; somatic mutation. Ref.6		VAR_041185
	Natural variant	672	1	L → P Ref.6		VAR_041186
	Natural variant	767	1	S → T: dbSNP rs17856748. Ref.6 Ref.3		VAR_025728
	Natural variant	767	1	S → Y in an ovarian papillary serous adenocarcinoma sample; somatic mutation. Ref.6 Ref.3		VAR_041187
	Natural variant	816	1	T → A Ref.6		VAR_041188
	Natural variant	839	1	R → Q Ref.6		VAR_041189
	Natural variant	840	1	L → V Ref.6		VAR_041190
	Natural variant	1003	1	G → D: dbSNP rs1863704. Ref.6 Ref.5		VAR_025729
	Natural variant	1111	1	Y → C Ref.6		VAR_041191
	Natural variant	1112	1	R → Q Ref.6		VAR_041192
	Natural variant	1138	1	Q → K in an ovarian serous carcinoma sample; somatic mutation. Ref.6		VAR_041193
	Natural variant	1185	1	P → S in an ovarian endometrioid sample; somatic mutation. Ref.6		VAR_041194
	Natural variant	1313	1	H → P Ref.6		VAR_041195
Experimental info
	Mutagenesis	33	1	K → R: No effect on nuclear localization of GLI1 or GLI2 or on GLI-mediated transcription. Ref.1
	Sequence conflict	229	1	N → D in AAH26158. Ref.3
	Sequence conflict	971 – 986	16	Missing Ref.5
	Sequence conflict	1003	1	G → V in AAF97028. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 21, 2006. Version 2. Checksum: 793F4638F1871C01	FASTA	1,315	143,995
	10 20 30 40 50 60 MEKYHVLEMI GEGSFGRVYK GRRKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLRH 70 80 90 100 110 120 PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR 130 140 150 160 170 180 ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT 190 200 210 220 230 240 ADLWSVGCIL YELAVGTPPF YATSIFQLVS LILKDPVRWP STISPCFKNF LQGLLTKDPR 250 260 270 280 290 300 QRLSWPDLLY HPFIAGHVTI ITEPAGPDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR 310 320 330 340 350 360 ILTQAYKRMA EEAMQKKHQN TGPALEQEDK TSKVAPGTAP LPRLGATPQE SSLLAGILAS 370 380 390 400 410 420 ELKSSWAKSG TGEVPSAPRE NRTTPDCERA FPEERPEVLG QRSTDVVDLE NEEPDSDNEW 430 440 450 460 470 480 QHLLETTEPV PIQLKAPLTL LCNPDFCQRI QSQLHEAGGQ ILKGILEGAS HILPAFRVLS 490 500 510 520 530 540 SLLSSCSDSV ALYSFCREAG LPGLLLSLLR HSQESNSLQQ QSWYGTFLQD LMAVIQAYFA 550 560 570 580 590 600 CTFNLERSQT SDSLQVFQEA ANLFLDLLGK LLAQPDDSEQ TLRRDSLMCF TVLCEAMDGN 610 620 630 640 650 660 SRAISKAFYS SLLTTQQVVL DGLLHGLTVP QLPVHTPQGA PQVSQPLREQ SEDIPGAISS 670 680 690 700 710 720 ALAAICTAPV GLPDCWDAKE QVCWHLANQL TEDSSQLRPS LISGLQHPIL CLHLLKVLYS 730 740 750 760 770 780 CCLVSEGLCR LLGQEPLALE SLFMLIQGKV KVVDWEESTE VTLYFLSLLV FRLQNLPCGM 790 800 810 820 830 840 EKLGSDVATL FTHSHVVSLV SAAACLLGQL GQQGVTFDLQ PMEWMAAATH ALSAPAEVRL 850 860 870 880 890 900 TPPGSCGFYD GLLILLLQLL TEQGKASLIR DMSSSEMWTV LWHRFSMVLR LPEEASAQEG 910 920 930 940 950 960 ELSLSSPPSP EPDWTLISPQ GMAALLSLAM ATFTQEPQLC LSCLSQHGSI LMSILKHLLC 970 980 990 1000 1010 1020 PSFLNQLRQA PHGSEFLPVV VLSVCQLLCF PFALDMDADL LIGVLADLRD SEVAAHLLQV 1030 1040 1050 1060 1070 1080 CCYHLPLMQV ELPISLLTRL ALMDPTSLNQ FVNTVSASPR TIVSFLSVAL LSDQPLLTSD 1090 1100 1110 1120 1130 1140 LLSLLAHTAR VLSPSHLSFI QELLAGSDES YRPLRSLLGH PENSVRAHTY RLLGHLLQHS 1150 1160 1170 1180 1190 1200 MALRGALQSQ SGLLSLLLLG LGDKDPVVRC SASFAVGNAA YQAGPLGPAL AAAVPSMTQL 1210 1220 1230 1240 1250 1260 LGDPQAGIRR NVASALGNLG PEGLGEELLQ CEVPQRLLEM ACGDPQPNVK EAALIALRSL 1270 1280 1290 1300 1310 QQEPGIHQVL VSLGASEKLS LLSLGNQSLP HSSPRPASAK HCRKLIHLLR PAHSM
	Isoform 2. Checksum: 42376671A0F272AF	FASTA	1,294	141,737
	10 20 30 40 50 60 MEKYHVLEMI GEGSFGRVYK GRRKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLRH 70 80 90 100 110 120 PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR 130 140 150 160 170 180 ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT 190 200 210 220 230 240 ADLWSVGCIL YELAVGTPPF YATSIFQLVS LILKDPVRWP STISPCFKNF LQGLLTKDPR 250 260 270 280 290 300 QRLSWPDLLY HPFIAGHVTI ITEPAGPDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR 310 320 330 340 350 360 ILTQAYKRMA EEAMQKKHQN TGPALEQEDK TSKVAPGTAP LPRLGATPQE SSLLAGILAS 370 380 390 400 410 420 ELKSSWAKSG TGEVPSAPRE NRTTPDCERA FPEERPEVLG QRSTDVVDLE NEEPDSDNEW 430 440 450 460 470 480 QHLLETTEPV PIQLKAPLTL LCNPDFCQRI QSQLHEAGGQ ILKGILEGAS HILPAFRVLS 490 500 510 520 530 540 SLLSSCSDSV ALYSFCREAG LPGLLLSLLR HSQESNSLQQ QSWYGTFLQD LMAVIQAYFA 550 560 570 580 590 600 CTFNLERSQT SDSLQVFQEA ANLFLDLLGK LLAQPDDSEQ TLRRDSLMCF TVLCEAMDGN 610 620 630 640 650 660 SRAISKAFYS SLLTTQQVVL DGLLHGLTVP QLPVHTPQGA PQVSQPLREQ SEDIPGAISS 670 680 690 700 710 720 ALAAICTAPV GLPDCWDAKE QVCWHLANQL TEDSSQLRPS LISGLQHPIL CLHLLKVLYS 730 740 750 760 770 780 CCLVSEGLCR LLGQEPLALE SLFMLIQGKV KVVDWEESTE VTLYFLSLLV FRLQNLPCGM 790 800 810 820 830 840 EKLGSDVATL FTHSHVVSLV SAAACLLGQL GQQGVTFDLQ PMEWMAAATH ALSAPAELLT 850 860 870 880 890 900 EQGKASLIRD MSSSEMWTVL WHRFSMVLRL PEEASAQEGE LSLSSPPSPE PDWTLISPQG 910 920 930 940 950 960 MAALLSLAMA TFTQEPQLCL SCLSQHGSIL MSILKHLLCP SFLNQLRQAP HGSEFLPVVV 970 980 990 1000 1010 1020 LSVCQLLCFP FALDMDADLL IGVLADLRDS EVAAHLLQVC CYHLPLMQVE LPISLLTRLA 1030 1040 1050 1060 1070 1080 LMDPTSLNQF VNTVSASPRT IVSFLSVALL SDQPLLTSDL LSLLAHTARV LSPSHLSFIQ 1090 1100 1110 1120 1130 1140 ELLAGSDESY RPLRSLLGHP ENSVRAHTYR LLGHLLQHSM ALRGALQSQS GLLSLLLLGL 1150 1160 1170 1180 1190 1200 GDKDPVVRCS ASFAVGNAAY QAGPLGPALA AAVPSMTQLL GDPQAGIRRN VASALGNLGP 1210 1220 1230 1240 1250 1260 EGLGEELLQC EVPQRLLEMA CGDPQPNVKE AALIALRSLQ QEPGIHQVLV SLGASEKLSL 1270 1280 1290 LSLGNQSLPH SSPRPASAKH CRKLIHLLRP AHSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gli regulation by the opposing activities of fused and suppressor of fused." Murone M., Luoh S.-L., Stone D., Li W., Gurney A., Armanini M., Grey C., Rosenthal A., de Sauvage F.J. Nat. Cell Biol. 2:310-312(2000) [PubMed: 10806483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH GLI1; GLI2; GLI3 AND SUFU, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-33.
[2]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed: 10574462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-463 AND THR-767. Tissue: Testis.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 823-1315 (ISOFORM 1). Tissue: Testis.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 839-1315 (ISOFORM 1), VARIANT ASP-1003. Tissue: Teratocarcinoma.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-90; TRP-240; ARG-295; ASN-329; VAL-462; SER-476; TRP-477; GLN-583; CYS-660; PRO-672; TYR-767; ALA-816; GLN-839; VAL-840; ASP-1003; CYS-1111; GLN-1112; LYS-1138; SER-1185 AND PRO-1313.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF200815 mRNA. Translation: AAF97028.1. AB033104 mRNA. Translation: BAA86592.1. Different initiation. BC026158 mRNA. Translation: AAH26158.1. AL133630 mRNA. Translation: CAB63754.1. AK022692 mRNA. Translation: BAB14184.1. Frameshift.
IPI	IPI00235407. IPI00735634.
PIR	T43465.
RefSeq	NP_056505.2.
UniGene	Hs.471404
3D structure databases
HSSP	HSSP built from PDB template 1OB3 based on UniProtKB Q07785.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NRP7. 8 interactions.
PTM databases
PhosphoSite	Q9NRP7.
Proteomic databases
PRIDE	Q9NRP7.
Genome annotation databases
Ensembl	ENSG00000163482. Homo sapiens. [Contig view]
GeneID	27148.
KEGG	hsa:27148.
NMPDR	fig|9606.3.peg.19335.
UCSC	uc002viu.1. human. uc002viv.1. human.
Organism-specific databases
GeneCards	GC02P219245.
HGNC	HGNC:17209. STK36.
MIM	607652. gene.
PharmGKB	PA38212.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NRP7.
HOVERGEN	Q9NRP7.
OMA	Q9NRP7. WTLISPQ.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Pathway_Interaction_DB	hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins.
Gene expression databases
ArrayExpress	Q9NRP7.
Bgee	Q9NRP7.
CleanEx	HS_STK36.
GermOnline	ENSG00000163482. Homo sapiens.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR000357. HEAT. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF02985. HEAT. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	49907.
SOURCE	Search...

Entry information

Entry name

STK36_HUMAN

Accession

Primary (citable) accession number: Q9NRP7
Secondary accession number(s): Q8TC32 , Q9H9N9, Q9UF35, Q9ULE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	March 21, 2006
Last modified:	July 7, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)