Reviewed,
UniProtKB/Swiss-Prot Q9NRP7 (STK36_HUMAN)
Last modified
July 7, 2009.
Version 70.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase 36 EC=2.7.11.1 Alternative name(s): Fused homolog | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1315 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Serine/threonine protein kinase required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Ref.1 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. UniProtKB P23647 |
| Cofactor | Magnesium. |
| Subcellular location | Cytoplasm. Nucleus. Note: Low levels also present in the nucleus. Ref.1 |
| Tissue specificity | Expressed at low levels in most fetal tissues, adult ovaries and at high levels in adult testis, where it is localized in germ cells. Ref.1 |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
| Sequence caution | The sequence BAB14184.1 differs from that shown. Reason: Frameshift at position 987. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ARMC6 | Q6NXE6 | 1 | EBI-863797,EBI-1053802 | |
| GLI1 | P08151 | 1 | EBI-863797,EBI-308084 | |
| Gli2 | Q8K0K3 | 1 | EBI-863797,EBI-308039 | From a different organism. |
| GLI3 | P10071 | 1 | EBI-863797,EBI-308055 | |
| SUFU | Q9UMX1 | 1 | EBI-863797,EBI-740595 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 Ref.1 Ref.3 Ref.5 Ref.4 (identifier: Q9NRP7-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 Ref.2 (identifier: Q9NRP7-2) The sequence of this isoform differs from the canonical sequence as follows: 838-858: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1315 | 1315 | Serine/threonine-protein kinase 36 | PRO_0000229020 | |||||
Regions | |||||||||
| Domain | 4 – 254 | 251 | Protein kinase | ||||||
| Nucleotide binding | 10 – 18 | 9 | ATP By similarity UniProtKB P23647 | ||||||
Sites | |||||||||
| Active site | 125 | 1 | Proton acceptor By similarity UniProtKB P23647 | ||||||
| Binding site | 33 | 1 | ATP UniProtKB P23647 | ||||||
Natural variations | |||||||||
| Alternative sequence | 838 – 858 | 21 | Missing in isoform 2. Ref.2 | VSP_051983 | |||||
| Natural variant | 90 | 1 | I → M Ref.6 | VAR_041177 | |||||
| Natural variant | 240 | 1 | R → W Ref.6 | VAR_041178 | |||||
| Natural variant | 295 | 1 | K → R Ref.6 | VAR_041179 | |||||
| Natural variant | 329 | 1 | D → N Ref.6 | VAR_041180 | |||||
| Natural variant | 462 | 1 | L → V Ref.6 | VAR_041181 | |||||
| Natural variant | 463 | 1 | K → N: dbSNP rs17856747. Ref.3 | VAR_025727 | |||||
| Natural variant | 476 | 1 | F → S Ref.6 | VAR_041182 | |||||
| Natural variant | 477 | 1 | R → W Ref.6 | VAR_041183 | |||||
| Natural variant | 583 | 1 | R → Q Ref.6 | VAR_041184 | |||||
| Natural variant | 638 | 1 | Q → P: dbSNP rs6709303. | VAR_057112 | |||||
| Natural variant | 660 | 1 | S → C in a breast pleomorphic lobular carcinoma sample; somatic mutation. Ref.6 | VAR_041185 | |||||
| Natural variant | 672 | 1 | L → P Ref.6 | VAR_041186 | |||||
| Natural variant | 767 | 1 | S → T: dbSNP rs17856748. Ref.6 Ref.3 | VAR_025728 | |||||
| Natural variant | 767 | 1 | S → Y in an ovarian papillary serous adenocarcinoma sample; somatic mutation. Ref.6 Ref.3 | VAR_041187 | |||||
| Natural variant | 816 | 1 | T → A Ref.6 | VAR_041188 | |||||
| Natural variant | 839 | 1 | R → Q Ref.6 | VAR_041189 | |||||
| Natural variant | 840 | 1 | L → V Ref.6 | VAR_041190 | |||||
| Natural variant | 1003 | 1 | G → D: dbSNP rs1863704. Ref.6 Ref.5 | VAR_025729 | |||||
| Natural variant | 1111 | 1 | Y → C Ref.6 | VAR_041191 | |||||
| Natural variant | 1112 | 1 | R → Q Ref.6 | VAR_041192 | |||||
| Natural variant | 1138 | 1 | Q → K in an ovarian serous carcinoma sample; somatic mutation. Ref.6 | VAR_041193 | |||||
| Natural variant | 1185 | 1 | P → S in an ovarian endometrioid sample; somatic mutation. Ref.6 | VAR_041194 | |||||
| Natural variant | 1313 | 1 | H → P Ref.6 | VAR_041195 | |||||
Experimental info | |||||||||
| Mutagenesis | 33 | 1 | K → R: No effect on nuclear localization of GLI1 or GLI2 or on GLI-mediated transcription. Ref.1 | ||||||
| Sequence conflict | 229 | 1 | N → D in AAH26158. Ref.3 | ||||||
| Sequence conflict | 971 – 986 | 16 | Missing Ref.5 | ||||||
| Sequence conflict | 1003 | 1 | G → V in AAF97028. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Gli regulation by the opposing activities of fused and suppressor of fused." Murone M., Luoh S.-L., Stone D., Li W., Gurney A., Armanini M., Grey C., Rosenthal A., de Sauvage F.J. Nat. Cell Biol. 2:310-312(2000) [PubMed: 10806483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH GLI1; GLI2; GLI3 AND SUFU, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-33. |
| [2] | "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed: 10574462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-463 AND THR-767. Tissue: Testis. |
| [4] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 823-1315 (ISOFORM 1). Tissue: Testis. |
| [5] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 839-1315 (ISOFORM 1), VARIANT ASP-1003. Tissue: Teratocarcinoma. |
| [6] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-90; TRP-240; ARG-295; ASN-329; VAL-462; SER-476; TRP-477; GLN-583; CYS-660; PRO-672; TYR-767; ALA-816; GLN-839; VAL-840; ASP-1003; CYS-1111; GLN-1112; LYS-1138; SER-1185 AND PRO-1313. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF200815 mRNA. Translation: AAF97028.1. AB033104 mRNA. Translation: BAA86592.1. Different initiation. BC026158 mRNA. Translation: AAH26158.1. AL133630 mRNA. Translation: CAB63754.1. AK022692 mRNA. Translation: BAB14184.1. Frameshift. | |
| IPI | IPI00235407. IPI00735634. |
| PIR | T43465. |
| RefSeq | NP_056505.2. |
| UniGene | Hs.471404 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OB3 based on UniProtKB Q07785. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9NRP7. 8 interactions. |
PTM databases | |
| PhosphoSite | Q9NRP7. |
Proteomic databases | |
| PRIDE | Q9NRP7. |
Genome annotation databases | |
| Ensembl | ENSG00000163482. Homo sapiens. [Contig view] |
| GeneID | 27148. |
| KEGG | hsa:27148. |
| NMPDR | fig|9606.3.peg.19335. |
| UCSC | uc002viu.1. human. uc002viv.1. human. |
Organism-specific databases | |
| GeneCards | GC02P219245. |
| HGNC | HGNC:17209. STK36. |
| MIM | 607652. gene. |
| PharmGKB | PA38212. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9NRP7. |
| HOVERGEN | Q9NRP7. |
| OMA | Q9NRP7. WTLISPQ. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 247. |
| Pathway_Interaction_DB | hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins. |
Gene expression databases | |
| ArrayExpress | Q9NRP7. |
| Bgee | Q9NRP7. |
| CleanEx | HS_STK36. |
| GermOnline | ENSG00000163482. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR011989. ARM-like. IPR000357. HEAT. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Gene3D | G3DSA:1.25.10.10. ARM-like. 1 hit. |
| Pfam | PF02985. HEAT. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 49907. |
| SOURCE | Search... |
Entry information
| Entry name | STK36_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9NRP7 Secondary accession number(s): Q8TC32 Q9ULE2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


