Q9NRN5 (OLFL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Olfactomedin-like protein 3

Alternative name(s):
HNOEL-iso
hOLF44

Gene names

Name:	OLFML3
ORF Names:	PSEC0035, PSEC0173, PSEC0244, UNQ663/PRO1294

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation By similarity. May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Abundant in placenta, moderate in liver and heart, whereas fairly weak in other tissues examined. On term placenta, mainly localized extracellularly surrounding the syncytiotrophoblastic cells and very rarely expressed in the maternal decidua layer. Ref.1
Sequence similarities	Belongs to the OLFML3 family. Contains 1 olfactomedin-like domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NRN5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NRN5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
     21-38: GQQHHLVEYMERRLAALE → MAQWHCSQRTQAGGHGVG

Isoform 3 (identifier: Q9NRN5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     134-287: DCGYTISQVR...ADEEGLWAVY → GKQSESRPLT...EDLRVRWDTE
     288-406: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 406	385	Olfactomedin-like protein 3		PRO_0000020092
Regions
	Domain	134 – 401	268	Olfactomedin-like
	Coiled coil	25 – 101	77	Potential
Amino acid modifications
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Disulfide bond	135 ↔ 328		By similarity
Natural variations
	Alternative sequence	1 – 20	20	Missing in isoform 2.		VSP_014128
	Alternative sequence	21 – 38	18	GQQHH…LAALE → MAQWHCSQRTQAGGHGVG in isoform 2.		VSP_014129
	Alternative sequence	134 – 287	154	DCGYT…LWAVY → GKQSESRPLTLLRTDILPSL LLTLFFTLSQSIVALSSGIC FRETSVMPGLLRRGSGDPGR CGTRSFSILPKIGGNRWANA HYSPDHPPWGSSCWRHLILA SISFSCAFLIRLWLHNLSSE INEDSEAIWWPSWSMDQGST GANREDLRVRWDTE in isoform 3.		VSP_014130
	Alternative sequence	288 – 406	119	Missing in isoform 3.		VSP_014131
Experimental info
	Sequence conflict	118	1	G → E in BAD96191. Ref.5
	Sequence conflict	131	1	M → K in BAD96941. Ref.5
	Sequence conflict	182	1	V → F in BAD96191. Ref.5
	Sequence conflict	277	1	A → V in AAQ89313. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0B5DB1A803BB99D6	FASTA	406	46,010
	10 20 30 40 50 60 MGPSTPLLIL FLLSWSGPLQ GQQHHLVEYM ERRLAALEER LAQCQDQSSR HAAELRDFKN 70 80 90 100 110 120 KMLPLLEVAE KEREALRTEA DTISGRVDRL EREVDYLETQ NPALPCVEFD EKVTGGPGTK 130 140 150 160 170 180 GKGRRNEKYD MVTDCGYTIS QVRSMKILKR FGGPAGLWTK DPLGQTEKIY VLDGTQNDTA 190 200 210 220 230 240 FVFPRLRDFT LAMAARKASR VRVPFPWVGT GQLVYGGFLY FARRPPGRPG GGGEMENTLQ 250 260 270 280 290 300 LIKFHLANRT VVDSSVFPAE GLIPPYGLTA DTYIDLAADE EGLWAVYATR EDDRHLCLAK 310 320 330 340 350 360 LDPQTLDTEQ QWDTPCPREN AEAAFVICGT LYVVYNTRPA SRARIQCSFD ASGTLTPERA 370 380 390 400 ALPYFPRRYG AHASLRYNPR ERQLYAWDDG YQIVYKLEMR KKEEEV
	Isoform 2 [UniParc]. Checksum: ACCD914542650DBD	FASTA	386	43,588
	10 20 30 40 50 60 MAQWHCSQRT QAGGHGVGER LAQCQDQSSR HAAELRDFKN KMLPLLEVAE KEREALRTEA 70 80 90 100 110 120 DTISGRVDRL EREVDYLETQ NPALPCVEFD EKVTGGPGTK GKGRRNEKYD MVTDCGYTIS 130 140 150 160 170 180 QVRSMKILKR FGGPAGLWTK DPLGQTEKIY VLDGTQNDTA FVFPRLRDFT LAMAARKASR 190 200 210 220 230 240 VRVPFPWVGT GQLVYGGFLY FARRPPGRPG GGGEMENTLQ LIKFHLANRT VVDSSVFPAE 250 260 270 280 290 300 GLIPPYGLTA DTYIDLAADE EGLWAVYATR EDDRHLCLAK LDPQTLDTEQ QWDTPCPREN 310 320 330 340 350 360 AEAAFVICGT LYVVYNTRPA SRARIQCSFD ASGTLTPERA ALPYFPRRYG AHASLRYNPR 370 380 ERQLYAWDDG YQIVYKLEMR KKEEEV
	Isoform 3 [UniParc]. Checksum: E5FA3E724F51D55B	FASTA	287	32,451
	10 20 30 40 50 60 MGPSTPLLIL FLLSWSGPLQ GQQHHLVEYM ERRLAALEER LAQCQDQSSR HAAELRDFKN 70 80 90 100 110 120 KMLPLLEVAE KEREALRTEA DTISGRVDRL EREVDYLETQ NPALPCVEFD EKVTGGPGTK 130 140 150 160 170 180 GKGRRNEKYD MVTGKQSESR PLTLLRTDIL PSLLLTLFFT LSQSIVALSS GICFRETSVM 190 200 210 220 230 240 PGLLRRGSGD PGRCGTRSFS ILPKIGGNRW ANAHYSPDHP PWGSSCWRHL ILASISFSCA 250 260 270 280 FLIRLWLHNL SSEINEDSEA IWWPSWSMDQ GSTGANREDL RVRWDTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hOLF44, a secreted glycoprotein with distinct expression pattern, belongs to an uncharacterized olfactomedin-like subfamily newly identified by phylogenetic analysis." Zeng L.-C., Liu F., Zhang X., Zhu Z.-D., Wang Z.-Q., Han Z.-G., Ma W.-J. FEBS Lett. 571:74-80(2004) [PubMed: 15280020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
[2]	"Novel genes expressed in human dendritic cells." Huang C., Ren S., Tu Y., Gu W., Wang Y., Han Z., Chen Z. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Dendritic cell.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Adipose tissue and Gastric mucosa.
[6]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY464015 mRNA. Translation: AAR88262.1. AF201945 mRNA. Translation: AAF86881.1. AY358954 mRNA. Translation: AAQ89313.1. AK075544 mRNA. Translation: BAC11687.1. AK223221 mRNA. Translation: BAD96941.1. AK222471 mRNA. Translation: BAD96191.1. AK075353 mRNA. Translation: BAC11564.1. AK075479 mRNA. Translation: BAC11644.1. AL731797 Genomic DNA. Translation: CAI22797.1. AL731797 Genomic DNA. Translation: CAI22798.1. BC009920 mRNA. Translation: AAH09920.1.
IPI	IPI00024621. IPI00385893. IPI00607652.
RefSeq	NP_064575.1. NM_020190.2.
UniGene	Hs.9315.
3D structure databases
ProteinModelPortal	Q9NRN5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NRN5. 4 interactions.
MINT	MINT-1385288.
STRING	Q9NRN5.
Polymorphism databases
DMDM	37999795.
Proteomic databases
PRIDE	Q9NRN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000320334; ENSP00000322273; ENSG00000116774.
GeneID	56944.
KEGG	hsa:56944.
UCSC	uc001eer.1. human. uc001ees.1. human.
Organism-specific databases
CTD	56944.
GeneCards	GC01P114522.
H-InvDB	HIX0000907.
HGNC	HGNC:24956. OLFML3.
MIM	610088. gene.
neXtProt	NX_Q9NRN5.
PharmGKB	PA134913777.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07781.
GeneTree	ENSGT00560000077036.
HOGENOM	HBG445753.
HOVERGEN	HBG108213.
InParanoid	Q9NRN5.
OMA	GPTEKIY.
OrthoDB	EOG43JC50.
PhylomeDB	Q9NRN5.
Gene expression databases
Bgee	Q9NRN5.
CleanEx	HS_OLFML3.
Genevestigator	Q9NRN5.
GermOnline	ENSG00000116774. Homo sapiens.
Family and domain databases
InterPro	IPR003112. Olfac-like. [Graphical view]
Pfam	PF02191. OLF. 1 hit. [Graphical view]
SMART	SM00284. OLF. 1 hit. [Graphical view]
PROSITE	PS51132. OLF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	62525.
SOURCE	Search...

Entry information

Entry name

OLFL3_HUMAN

Accession

Primary (citable) accession number: Q9NRN5
Secondary accession number(s): Q53FR1 , Q53HV9, Q5SQL6, Q69AX9, Q8NBJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.