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Protein

Olfactomedin-like protein 3

Gene

OLFML3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Olfactomedin-like protein 3
Alternative name(s):
HNOEL-iso
hOLF44
Gene namesi
Name:OLFML3
ORF Names:PSEC0035, PSEC0173, PSEC0244, UNQ663/PRO1294
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24956. OLFML3.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134913777.

Polymorphism and mutation databases

DMDMi37999795.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 406385Olfactomedin-like protein 3PRO_0000020092Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi135 ↔ 328PROSITE-ProRule annotation
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence analysis
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NRN5.
PeptideAtlasiQ9NRN5.
PRIDEiQ9NRN5.

PTM databases

iPTMnetiQ9NRN5.
PhosphoSiteiQ9NRN5.

Expressioni

Tissue specificityi

Abundant in placenta, moderate in liver and heart, whereas fairly weak in other tissues examined. On term placenta, mainly localized extracellularly surrounding the syncytiotrophoblastic cells and very rarely expressed in the maternal decidua layer.1 Publication

Gene expression databases

BgeeiQ9NRN5.
CleanExiHS_OLFML3.
ExpressionAtlasiQ9NRN5. baseline and differential.
GenevisibleiQ9NRN5. HS.

Organism-specific databases

HPAiHPA056362.

Interactioni

Protein-protein interaction databases

BioGridi121268. 7 interactions.
IntActiQ9NRN5. 5 interactions.
MINTiMINT-1385288.
STRINGi9606.ENSP00000322273.

Structurei

3D structure databases

ProteinModelPortaliQ9NRN5.
SMRiQ9NRN5. Positions 147-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 401268Olfactomedin-likePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 10177Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the OLFML3 family.Curated
Contains 1 olfactomedin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG3545. Eukaryota.
ENOG410ZJ72. LUCA.
GeneTreeiENSGT00760000119005.
HOGENOMiHOG000115263.
HOVERGENiHBG108213.
InParanoidiQ9NRN5.
OMAiEGLWAIY.
OrthoDBiEOG7ZGX32.
PhylomeDBiQ9NRN5.
TreeFamiTF352000.

Family and domain databases

InterProiIPR003112. Olfac-like_dom.
IPR031230. OLFML3.
[Graphical view]
PANTHERiPTHR23192:SF8. PTHR23192:SF8. 1 hit.
PfamiPF02191. OLF. 1 hit.
[Graphical view]
SMARTiSM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS51132. OLF. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NRN5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPSTPLLIL FLLSWSGPLQ GQQHHLVEYM ERRLAALEER LAQCQDQSSR
60 70 80 90 100
HAAELRDFKN KMLPLLEVAE KEREALRTEA DTISGRVDRL EREVDYLETQ
110 120 130 140 150
NPALPCVEFD EKVTGGPGTK GKGRRNEKYD MVTDCGYTIS QVRSMKILKR
160 170 180 190 200
FGGPAGLWTK DPLGQTEKIY VLDGTQNDTA FVFPRLRDFT LAMAARKASR
210 220 230 240 250
VRVPFPWVGT GQLVYGGFLY FARRPPGRPG GGGEMENTLQ LIKFHLANRT
260 270 280 290 300
VVDSSVFPAE GLIPPYGLTA DTYIDLAADE EGLWAVYATR EDDRHLCLAK
310 320 330 340 350
LDPQTLDTEQ QWDTPCPREN AEAAFVICGT LYVVYNTRPA SRARIQCSFD
360 370 380 390 400
ASGTLTPERA ALPYFPRRYG AHASLRYNPR ERQLYAWDDG YQIVYKLEMR

KKEEEV
Length:406
Mass (Da):46,010
Last modified:October 1, 2000 - v1
Checksum:i0B5DB1A803BB99D6
GO
Isoform 2 (identifier: Q9NRN5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
     21-38: GQQHHLVEYMERRLAALE → MAQWHCSQRTQAGGHGVG

Show »
Length:386
Mass (Da):43,588
Checksum:iACCD914542650DBD
GO
Isoform 3 (identifier: Q9NRN5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-287: DCGYTISQVR...ADEEGLWAVY → GKQSESRPLT...EDLRVRWDTE
     288-406: Missing.

Show »
Length:287
Mass (Da):32,451
Checksum:iE5FA3E724F51D55B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181G → E in BAD96191 (Ref. 5) Curated
Sequence conflicti131 – 1311M → K in BAD96941 (Ref. 5) Curated
Sequence conflicti182 – 1821V → F in BAD96191 (Ref. 5) Curated
Sequence conflicti277 – 2771A → V in AAQ89313 (PubMed:12975309).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020Missing in isoform 2. CuratedVSP_014128Add
BLAST
Alternative sequencei21 – 3818GQQHH…LAALE → MAQWHCSQRTQAGGHGVG in isoform 2. CuratedVSP_014129Add
BLAST
Alternative sequencei134 – 287154DCGYT…LWAVY → GKQSESRPLTLLRTDILPSL LLTLFFTLSQSIVALSSGIC FRETSVMPGLLRRGSGDPGR CGTRSFSILPKIGGNRWANA HYSPDHPPWGSSCWRHLILA SISFSCAFLIRLWLHNLSSE INEDSEAIWWPSWSMDQGST GANREDLRVRWDTE in isoform 3. 1 PublicationVSP_014130Add
BLAST
Alternative sequencei288 – 406119Missing in isoform 3. 1 PublicationVSP_014131Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY464015 mRNA. Translation: AAR88262.1.
AF201945 mRNA. Translation: AAF86881.1.
AY358954 mRNA. Translation: AAQ89313.1.
AK075544 mRNA. Translation: BAC11687.1.
AK223221 mRNA. Translation: BAD96941.1.
AK222471 mRNA. Translation: BAD96191.1.
AK075353 mRNA. Translation: BAC11564.1.
AK075479 mRNA. Translation: BAC11644.1.
AL731797 Genomic DNA. Translation: CAI22797.1.
AL731797 Genomic DNA. Translation: CAI22798.1.
BC009920 mRNA. Translation: AAH09920.1.
CCDSiCCDS65618.1. [Q9NRN5-2]
CCDS870.1. [Q9NRN5-1]
RefSeqiNP_001273281.1. NM_001286352.1. [Q9NRN5-2]
NP_064575.1. NM_020190.3. [Q9NRN5-1]
UniGeneiHs.9315.

Genome annotation databases

EnsembliENST00000320334; ENSP00000322273; ENSG00000116774. [Q9NRN5-1]
ENST00000369551; ENSP00000358564; ENSG00000116774. [Q9NRN5-2]
GeneIDi56944.
KEGGihsa:56944.
UCSCiuc001eer.3. human. [Q9NRN5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY464015 mRNA. Translation: AAR88262.1.
AF201945 mRNA. Translation: AAF86881.1.
AY358954 mRNA. Translation: AAQ89313.1.
AK075544 mRNA. Translation: BAC11687.1.
AK223221 mRNA. Translation: BAD96941.1.
AK222471 mRNA. Translation: BAD96191.1.
AK075353 mRNA. Translation: BAC11564.1.
AK075479 mRNA. Translation: BAC11644.1.
AL731797 Genomic DNA. Translation: CAI22797.1.
AL731797 Genomic DNA. Translation: CAI22798.1.
BC009920 mRNA. Translation: AAH09920.1.
CCDSiCCDS65618.1. [Q9NRN5-2]
CCDS870.1. [Q9NRN5-1]
RefSeqiNP_001273281.1. NM_001286352.1. [Q9NRN5-2]
NP_064575.1. NM_020190.3. [Q9NRN5-1]
UniGeneiHs.9315.

3D structure databases

ProteinModelPortaliQ9NRN5.
SMRiQ9NRN5. Positions 147-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121268. 7 interactions.
IntActiQ9NRN5. 5 interactions.
MINTiMINT-1385288.
STRINGi9606.ENSP00000322273.

PTM databases

iPTMnetiQ9NRN5.
PhosphoSiteiQ9NRN5.

Polymorphism and mutation databases

DMDMi37999795.

Proteomic databases

PaxDbiQ9NRN5.
PeptideAtlasiQ9NRN5.
PRIDEiQ9NRN5.

Protocols and materials databases

DNASUi56944.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000320334; ENSP00000322273; ENSG00000116774. [Q9NRN5-1]
ENST00000369551; ENSP00000358564; ENSG00000116774. [Q9NRN5-2]
GeneIDi56944.
KEGGihsa:56944.
UCSCiuc001eer.3. human. [Q9NRN5-1]

Organism-specific databases

CTDi56944.
GeneCardsiOLFML3.
H-InvDBHIX0160021.
HGNCiHGNC:24956. OLFML3.
HPAiHPA056362.
MIMi610088. gene.
neXtProtiNX_Q9NRN5.
PharmGKBiPA134913777.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3545. Eukaryota.
ENOG410ZJ72. LUCA.
GeneTreeiENSGT00760000119005.
HOGENOMiHOG000115263.
HOVERGENiHBG108213.
InParanoidiQ9NRN5.
OMAiEGLWAIY.
OrthoDBiEOG7ZGX32.
PhylomeDBiQ9NRN5.
TreeFamiTF352000.

Miscellaneous databases

GeneWikiiOLFML3.
GenomeRNAii56944.
PROiQ9NRN5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NRN5.
CleanExiHS_OLFML3.
ExpressionAtlasiQ9NRN5. baseline and differential.
GenevisibleiQ9NRN5. HS.

Family and domain databases

InterProiIPR003112. Olfac-like_dom.
IPR031230. OLFML3.
[Graphical view]
PANTHERiPTHR23192:SF8. PTHR23192:SF8. 1 hit.
PfamiPF02191. OLF. 1 hit.
[Graphical view]
SMARTiSM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS51132. OLF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "hOLF44, a secreted glycoprotein with distinct expression pattern, belongs to an uncharacterized olfactomedin-like subfamily newly identified by phylogenetic analysis."
    Zeng L.-C., Liu F., Zhang X., Zhu Z.-D., Wang Z.-Q., Han Z.-G., Ma W.-J.
    FEBS Lett. 571:74-80(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
  2. "Novel genes expressed in human dendritic cells."
    Huang C., Ren S., Tu Y., Gu W., Wang Y., Han Z., Chen Z.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Dendritic cell.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Adipose tissue and Gastric mucosa.
  6. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Teratocarcinoma.
  7. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Muscle.

Entry informationi

Entry nameiOLFL3_HUMAN
AccessioniPrimary (citable) accession number: Q9NRN5
Secondary accession number(s): Q53FR1
, Q53HV9, Q5SQL6, Q69AX9, Q8NBJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.