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Protein

Bromodomain adjacent to zinc finger domain protein 1A

Gene

BAZ1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1148 – 1198PHD-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

  • chromatin remodeling Source: BHF-UCL
  • DNA-dependent DNA replication Source: UniProtKB
  • regulation of transcription, DNA-templated Source: BHF-UCL
  • transcription, DNA-templated Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-30985-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain adjacent to zinc finger domain protein 1A
Alternative name(s):
ATP-dependent chromatin-remodeling protein
ATP-utilizing chromatin assembly and remodeling factor 1
Short name:
hACF1
CHRAC subunit ACF1
Williams syndrome transcription factor-related chromatin-remodeling factor 180
Short name:
WCRF180
hWALp1
Gene namesi
Name:BAZ1A
Synonyms:ACF1, WCRF180
ORF Names:HSPC317
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:960. BAZ1A.

Subcellular locationi

  • Nucleus

  • Note: May target the CHRAC complex to heterochromatin.

GO - Cellular componenti

  • ACF complex Source: BHF-UCL
  • CHRAC Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11177.
OpenTargetsiENSG00000198604.
PharmGKBiPA25270.

Polymorphism and mutation databases

BioMutaiBAZ1A.
DMDMi116241266.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002111671 – 1556Bromodomain adjacent to zinc finger domain protein 1AAdd BLAST1556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei270PhosphoserineCombined sources1
Modified residuei702PhosphoserineCombined sources1
Modified residuei731PhosphothreonineCombined sources1
Modified residuei960PhosphoserineBy similarity1
Modified residuei961PhosphoserineCombined sources1
Modified residuei1281PhosphoserineCombined sources1
Modified residuei1320PhosphoserineCombined sources1
Modified residuei1339PhosphoserineBy similarity1
Modified residuei1353PhosphoserineBy similarity1
Modified residuei1363PhosphoserineCombined sources1
Modified residuei1371PhosphoserineCombined sources1
Modified residuei1402PhosphoserineCombined sources1
Modified residuei1413PhosphoserineCombined sources1
Modified residuei1417PhosphoserineCombined sources1
Modified residuei1547PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NRL2.
MaxQBiQ9NRL2.
PaxDbiQ9NRL2.
PeptideAtlasiQ9NRL2.
PRIDEiQ9NRL2.

PTM databases

iPTMnetiQ9NRL2.
PhosphoSitePlusiQ9NRL2.

Expressioni

Tissue specificityi

Highly expressed in testis and at low or undetectable levels in other tissues analyzed.

Gene expression databases

BgeeiENSG00000198604.
CleanExiHS_BAZ1A.
ExpressionAtlasiQ9NRL2. baseline and differential.
GenevisibleiQ9NRL2. HS.

Organism-specific databases

HPAiHPA002730.

Interactioni

Subunit structurei

Component of the ACF chromatin remodeling complex that includes BAZ1A and SMARCA5. Additional this complex can form, together with CHRAC1 and POLE1, the histone-fold protein complex, CHRAC. Interacts with NCOR1 (via its RD1 domain); the interaction corepresses a number of NCOR1-regulated genes.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HTTP428584EBI-927511,EBI-466029
SMARCA5O602643EBI-927511,EBI-352588

Protein-protein interaction databases

BioGridi116347. 34 interactors.
DIPiDIP-36071N.
IntActiQ9NRL2. 18 interactors.
MINTiMINT-1183777.
STRINGi9606.ENSP00000353458.

Structurei

3D structure databases

ProteinModelPortaliQ9NRL2.
SMRiQ9NRL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 128WACPROSITE-ProRule annotationAdd BLAST107
Domaini422 – 487DDTPROSITE-ProRule annotationAdd BLAST66
Domaini1446 – 1516BromoPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 133Required for interaction with NCOR11 PublicationAdd BLAST133
Regioni1 – 128Required for association with the CHRAC1/POLE3 complexAdd BLAST128
Regioni667 – 933Interaction with SMARCA5Add BLAST267

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili306 – 397Sequence analysisAdd BLAST92
Coiled coili634 – 709Sequence analysisAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi487 – 491Poly-Glu5
Compositional biasi1239 – 1257Glu-richAdd BLAST19

Sequence similaritiesi

Belongs to the WAL family.Curated
Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 DDT domain.PROSITE-ProRule annotation
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation
Contains 1 WAC domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1148 – 1198PHD-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Bromodomain, Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1245. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119099.
HOGENOMiHOG000095179.
HOVERGENiHBG080889.
InParanoidiQ9NRL2.
KOiK11655.
OMAiKDMEKWR.
OrthoDBiEOG091G06JE.
PhylomeDBiQ9NRL2.
TreeFamiTF316326.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR018501. DDT_dom.
IPR028942. WHIM1_dom.
IPR028941. WHIM2_dom.
IPR013136. WSTF_Acf1_Cbp146.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF02791. DDT. 1 hit.
PF00628. PHD. 1 hit.
PF10537. WAC_Acf1_DNA_bd. 1 hit.
PF15612. WHIM1. 1 hit.
PF15613. WSD. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
SM00571. DDT. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS50827. DDT. 1 hit.
PS51136. WAC. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NRL2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLLHRKPFV RQKPPADLRP DEEVFYCKVT NEIFRHYDDF FERTILCNSL
60 70 80 90 100
VWSCAVTGRP GLTYQEALES EKKARQNLQS FPEPLIIPVL YLTSLTHRSR
110 120 130 140 150
LHEICDDIFA YVKDRYFVEE TVEVIRNNGA RLQCRILEVL PPSHQNGFAN
160 170 180 190 200
GHVNSVDGET IIISDSDDSE TQSCSFQNGK KKDAIDPLLF KYKVQPTKKE
210 220 230 240 250
LHESAIVKAT QISRRKHLFS RDKLKLFLKQ HCEPQDGVIK IKASSLSTYK
260 270 280 290 300
IAEQDFSYFF PDDPPTFIFS PANRRRGRPP KRIHISQEDN VANKQTLASY
310 320 330 340 350
RSKATKERDK LLKQEEMKSL AFEKAKLKRE KADALEAKKK EKEDKEKKRE
360 370 380 390 400
ELKKIVEEER LKKKEEKERL KVEREKEREK LREEKRKYVE YLKQWSKPRE
410 420 430 440 450
DMECDDLKEL PEPTPVKTRL PPEIFGDALM VLEFLNAFGE LFDLQDEFPD
460 470 480 490 500
GVTLEVLEEA LVGNDSEGPL CELLFFFLTA IFQAIAEEEE EVAKEQLTDA
510 520 530 540 550
DTKDLTEALD EDADPTKSAL SAVASLAAAW PQLHQGCSLK SLDLDSCTLS
560 570 580 590 600
EILRLHILAS GADVTSANAK YRYQKRGGFD ATDDACMELR LSNPSLVKKL
610 620 630 640 650
SSTSVYDLTP GEKMKILHAL CGKLLTLVST RDFIEDYVDI LRQAKQEFRE
660 670 680 690 700
LKAEQHRKER EEAAARIRKR KEEKLKEQEQ KMKEKQEKLK EDEQRNSTAD
710 720 730 740 750
ISIGEEERED FDTSIESKDT EQKELDQDMV TEDEDDPGSH KRGRRGKRGQ
760 770 780 790 800
NGFKEFTRQE QINCVTREPL TADEEEALKQ EHQRKEKELL EKIQSAIACT
810 820 830 840 850
NIFPLGRDRM YRRYWIFPSI PGLFIEEDYS GLTEDMLLPR PSSFQNNVQS
860 870 880 890 900
QDPQVSTKTG EPLMSESTSN IDQGPRDHSV QLPKPVHKPN RWCFYSSCEQ
910 920 930 940 950
LDQLIEALNS RGHRESALKE TLLQEKSRIC AQLARFSEEK FHFSDKPQPD
960 970 980 990 1000
SKPTYSRGRS SNAYDPSQMC AEKQLELRLR DFLLDIEDRI YQGTLGAIKV
1010 1020 1030 1040 1050
TDRHIWRSAL ESGRYELLSE ENKENGIIKT VNEDVEEMEI DEQTKVIVKD
1060 1070 1080 1090 1100
RLLGIKTETP STVSTNASTP QSVSSVVHYL AMALFQIEQG IERRFLKAPL
1110 1120 1130 1140 1150
DASDSGRSYK TVLDRWRESL LSSASLSQVF LHLSTLDRSV IWSKSILNAR
1160 1170 1180 1190 1200
CKICRKKGDA ENMVLCDGCD RGHHTYCVRP KLKTVPEGDW FCPECRPKQR
1210 1220 1230 1240 1250
SRRLSSRQRP SLESDEDVED SMGGEDDEVD GDEEEGQSEE EEYEVEQDED
1260 1270 1280 1290 1300
DSQEEEEVSL PKRGRPQVRL PVKTRGKLSS SFSSRGQQQE PGRYPSRSQQ
1310 1320 1330 1340 1350
STPKTTVSSK TGRSLRKINS APPTETKSLR IASRSTRHSH GPLQADVFVE
1360 1370 1380 1390 1400
LLSPRRKRRG RKSANNTPEN SPNFPNFRVI ATKSSEQSRS VNIASKLSLQ
1410 1420 1430 1440 1450
ESESKRRCRK RQSPEPSPVT LGRRSSGRQG GVHELSAFEQ LVVELVRHDD
1460 1470 1480 1490 1500
SWPFLKLVSK IQVPDYYDII KKPIALNIIR EKVNKCEYKL ASEFIDDIEL
1510 1520 1530 1540 1550
MFSNCFEYNP RNTSEAKAGT RLQAFFHIQA QKLGLHVTPS NVDQVSTPPA

AKKSRI
Length:1,556
Mass (Da):178,702
Last modified:October 17, 2006 - v2
Checksum:i4D78B9A8ADBF715B
GO
Isoform 2 (identifier: Q9NRL2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-535: Missing.

Show »
Length:1,524
Mass (Da):175,385
Checksum:i2DB787369178282B
GO

Sequence cautioni

The sequence AAF28995 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135R → T in BAA89209 (PubMed:10662543).Curated1
Sequence conflicti236D → E in BAA89209 (PubMed:10662543).Curated1
Sequence conflicti494K → Q in AAF28995 (Ref. 6) Curated1
Sequence conflicti503 – 508KDLTEA → QDFTEP in AAF28995 (Ref. 6) Curated6
Sequence conflicti525S → P in AAF28995 (Ref. 6) Curated1
Sequence conflicti536 – 540GCSLK → AALKF in AAF28995 (Ref. 6) Curated5
Sequence conflicti551E → D in AAF70601 (PubMed:10880450).Curated1
Sequence conflicti730V → F in BAA89209 (PubMed:10662543).Curated1
Sequence conflicti769P → L in BAA89209 (PubMed:10662543).Curated1
Sequence conflicti1201S → C in BAA89209 (PubMed:10662543).Curated1
Sequence conflicti1206S → F in BAA89209 (PubMed:10662543).Curated1
Sequence conflicti1409R → K in AAF70601 (PubMed:10880450).Curated1
Sequence conflicti1409R → K in CAB43261 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028049344D → E.Corresponds to variant rs1133285dbSNPEnsembl.1
Natural variantiVAR_0484231366N → K.2 PublicationsCorresponds to variant rs1044140dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000551504 – 535Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF213467 mRNA. Translation: AAF70601.1.
AF221130 mRNA. Translation: AAF32366.1.
AB032252 mRNA. Translation: BAA89209.1.
AL121603 Genomic DNA. No translation available.
AL355885 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65900.1.
AF161435 mRNA. Translation: AAF28995.1. Frameshift.
AL050089 mRNA. Translation: CAB43261.1.
CCDSiCCDS41943.1. [Q9NRL2-2]
CCDS9651.1. [Q9NRL2-1]
PIRiT08738.
RefSeqiNP_038476.2. NM_013448.2. [Q9NRL2-1]
NP_872589.1. NM_182648.1. [Q9NRL2-2]
UniGeneiHs.509140.

Genome annotation databases

EnsembliENST00000358716; ENSP00000351555; ENSG00000198604. [Q9NRL2-2]
ENST00000360310; ENSP00000353458; ENSG00000198604. [Q9NRL2-1]
ENST00000382422; ENSP00000371859; ENSG00000198604. [Q9NRL2-1]
GeneIDi11177.
KEGGihsa:11177.
UCSCiuc001wsk.4. human. [Q9NRL2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF213467 mRNA. Translation: AAF70601.1.
AF221130 mRNA. Translation: AAF32366.1.
AB032252 mRNA. Translation: BAA89209.1.
AL121603 Genomic DNA. No translation available.
AL355885 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65900.1.
AF161435 mRNA. Translation: AAF28995.1. Frameshift.
AL050089 mRNA. Translation: CAB43261.1.
CCDSiCCDS41943.1. [Q9NRL2-2]
CCDS9651.1. [Q9NRL2-1]
PIRiT08738.
RefSeqiNP_038476.2. NM_013448.2. [Q9NRL2-1]
NP_872589.1. NM_182648.1. [Q9NRL2-2]
UniGeneiHs.509140.

3D structure databases

ProteinModelPortaliQ9NRL2.
SMRiQ9NRL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116347. 34 interactors.
DIPiDIP-36071N.
IntActiQ9NRL2. 18 interactors.
MINTiMINT-1183777.
STRINGi9606.ENSP00000353458.

PTM databases

iPTMnetiQ9NRL2.
PhosphoSitePlusiQ9NRL2.

Polymorphism and mutation databases

BioMutaiBAZ1A.
DMDMi116241266.

Proteomic databases

EPDiQ9NRL2.
MaxQBiQ9NRL2.
PaxDbiQ9NRL2.
PeptideAtlasiQ9NRL2.
PRIDEiQ9NRL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358716; ENSP00000351555; ENSG00000198604. [Q9NRL2-2]
ENST00000360310; ENSP00000353458; ENSG00000198604. [Q9NRL2-1]
ENST00000382422; ENSP00000371859; ENSG00000198604. [Q9NRL2-1]
GeneIDi11177.
KEGGihsa:11177.
UCSCiuc001wsk.4. human. [Q9NRL2-1]

Organism-specific databases

CTDi11177.
DisGeNETi11177.
GeneCardsiBAZ1A.
H-InvDBHIX0037904.
HGNCiHGNC:960. BAZ1A.
HPAiHPA002730.
MIMi605680. gene.
neXtProtiNX_Q9NRL2.
OpenTargetsiENSG00000198604.
PharmGKBiPA25270.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1245. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119099.
HOGENOMiHOG000095179.
HOVERGENiHBG080889.
InParanoidiQ9NRL2.
KOiK11655.
OMAiKDMEKWR.
OrthoDBiEOG091G06JE.
PhylomeDBiQ9NRL2.
TreeFamiTF316326.

Enzyme and pathway databases

BioCyciZFISH:G66-30985-MONOMER.

Miscellaneous databases

ChiTaRSiBAZ1A. human.
GenomeRNAii11177.
PROiQ9NRL2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198604.
CleanExiHS_BAZ1A.
ExpressionAtlasiQ9NRL2. baseline and differential.
GenevisibleiQ9NRL2. HS.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR018501. DDT_dom.
IPR028942. WHIM1_dom.
IPR028941. WHIM2_dom.
IPR013136. WSTF_Acf1_Cbp146.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF02791. DDT. 1 hit.
PF00628. PHD. 1 hit.
PF10537. WAC_Acf1_DNA_bd. 1 hit.
PF15612. WHIM1. 1 hit.
PF15613. WSD. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
SM00571. DDT. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS50827. DDT. 1 hit.
PS51136. WAC. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAZ1A_HUMAN
AccessioniPrimary (citable) accession number: Q9NRL2
Secondary accession number(s): Q9NZ15
, Q9P065, Q9UIG1, Q9Y3V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Stimulated by double-stranded DNA and nucleosomal DNA.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.