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Q9NRJ5 (PAPOB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase beta

Short name=PAP-beta
EC=2.7.7.19
Alternative name(s):
Polynucleotide adenylyltransferase beta
Testis-specific poly(A) polymerase
Gene names
Name:PAPOLB
Synonyms:PAPT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length636 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subunit structure

Interacts with GSG1 By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Testis specific.

Sequence similarities

Belongs to the poly(A) polymerase family.

Sequence caution

The sequence AAH36653.2 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 636636Poly(A) polymerase beta
PRO_0000051622

Regions

Nucleotide binding100 – 1023ATP By similarity
Nucleotide binding113 – 1153ATP By similarity
Nucleotide binding246 – 2472ATP By similarity

Sites

Metal binding1131Magnesium 1; catalytic By similarity
Metal binding1131Magnesium 2; catalytic By similarity
Metal binding1151Magnesium 1; catalytic By similarity
Metal binding1151Magnesium 2; catalytic By similarity
Metal binding1671Magnesium 2; catalytic By similarity
Binding site1091ATP By similarity
Binding site1671ATP By similarity
Binding site2281ATP By similarity
Binding site2371ATP By similarity
Site1531Interaction with RNA By similarity
Site1581Interaction with RNA By similarity
Site3281Interaction with RNA By similarity
Site3991Interaction with RNA By similarity
Site5241Interaction with RNA By similarity

Experimental info

Sequence conflict661E → D in AAH36653. Ref.3
Sequence conflict1501E → Q in AAH36653. Ref.3
Sequence conflict4861M → V in AAH36653. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9NRJ5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 3A0823F4309C35DD

FASTA63671,681
        10         20         30         40         50         60 
MPFPVTTQGP PQPAPPPNRY GVSSPISLAV PKETDCLLTQ RLIETLRPFG VFEEEEELQR 

        70         80         90        100        110        120 
RILVLEKLNN LVKEWIREIS ESKSLPQSVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA 

       130        140        150        160        170        180 
PSHVDRSDFF TSFYAKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE 

       190        200        210        220        230        240 
DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKCHNIYSNI 

       250        260        270        280        290        300 
LGFLGGVSWA MLVARTCQLY PNAVASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW 

       310        320        330        340        350        360 
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS ISTRMVMIEE FKQGLAITHE ILLSKAEWSK 

       370        380        390        400        410        420 
LFEAPSFFQK YKHYIVLLAS ASTEKQHLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS 

       430        440        450        460        470        480 
FPAPKENPDM EEFRTMWVIG LGLKKPDNSE ILSIDLTYDI QSFTDTVYRQ AVNSKMFEMG 

       490        500        510        520        530        540 
MKITAMHLRR KELHQLLPHH VLQDKKAHST EGRRLTDLND SSFDLSAGCE NSMSVPSSTS 

       550        560        570        580        590        600 
TMKTGPLISS SQGRNSPALA VMTASVANIQ ATEFSLQQVN TNESSGVALN ESIPHAVSQP 

       610        620        630 
AISPSPKAMV ARVVSSTCLI SHPDLQETQQ QTYLIL 

« Hide

References

« Hide 'large scale' references
[1]"Testis-specific poly(A) polymerase gene is conserved in human and mouse."
Lee Y., Kim H., Chung J., Lee Y.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF218840 mRNA. Translation: AAF81013.1.
AC092610 Genomic DNA. Translation: AAS07561.1.
BC036653 mRNA. Translation: AAH36653.2. Different initiation.
RefSeqNP_064529.4. NM_020144.4.
UniGeneHs.487409.

3D structure databases

ProteinModelPortalQ9NRJ5.
SMRQ9NRJ5. Positions 20-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121233. 1 interaction.
STRING9606.ENSP00000384700.

PTM databases

PhosphoSiteQ9NRJ5.

Polymorphism databases

DMDM18203318.

Proteomic databases

PaxDbQ9NRJ5.
PRIDEQ9NRJ5.

Protocols and materials databases

DNASU56903.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000404991; ENSP00000384700; ENSG00000218823.
GeneID56903.
KEGGhsa:56903.

Organism-specific databases

CTD56903.
GeneCardsGC07M004897.
HGNCHGNC:15970. PAPOLB.
HPAHPA054427.
MIM607436. gene.
neXtProtNX_Q9NRJ5.
PharmGKBPA32933.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5186.
HOVERGENHBG053502.
InParanoidQ9NRJ5.
KOK14376.
OMAMSDGMAR.
OrthoDBEOG7XH6PT.
TreeFamTF300842.

Gene expression databases

ArrayExpressQ9NRJ5.
BgeeQ9NRJ5.
CleanExHS_PAPOLB.
GenevestigatorQ9NRJ5.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi56903.
NextBio62363.
PROQ9NRJ5.
SOURCESearch...

Entry information

Entry namePAPOB_HUMAN
AccessionPrimary (citable) accession number: Q9NRJ5
Secondary accession number(s): Q75LH1, Q8NE14
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM