SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9NRJ5

- PAPOB_HUMAN

UniProt

Q9NRJ5 - PAPOB_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Poly(A) polymerase beta

Gene
PAPOLB, PAPT
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATP By similarity
Metal bindingi113 – 1131Magnesium 1; catalytic By similarity
Metal bindingi113 – 1131Magnesium 2; catalytic By similarity
Metal bindingi115 – 1151Magnesium 1; catalytic By similarity
Metal bindingi115 – 1151Magnesium 2; catalytic By similarity
Sitei153 – 1531Interaction with RNA By similarity
Sitei158 – 1581Interaction with RNA By similarity
Metal bindingi167 – 1671Magnesium 2; catalytic By similarity
Binding sitei167 – 1671ATP By similarity
Binding sitei228 – 2281ATP By similarity
Binding sitei237 – 2371ATP By similarity
Sitei328 – 3281Interaction with RNA By similarity
Sitei399 – 3991Interaction with RNA By similarity
Sitei524 – 5241Interaction with RNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1023ATP By similarity
Nucleotide bindingi113 – 1153ATP By similarity
Nucleotide bindingi246 – 2472ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: RefGenome
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: RefGenome
  2. mRNA splicing, via spliceosome Source: RefGenome
  3. termination of RNA polymerase II transcription Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase beta (EC:2.7.7.19)
Short name:
PAP-beta
Alternative name(s):
Polynucleotide adenylyltransferase beta
Testis-specific poly(A) polymerase
Gene namesi
Name:PAPOLB
Synonyms:PAPT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:15970. PAPOLB.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. endoplasmic reticulum Source: Ensembl
  3. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32933.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 636636Poly(A) polymerase betaPRO_0000051622Add
BLAST

Proteomic databases

PaxDbiQ9NRJ5.
PRIDEiQ9NRJ5.

PTM databases

PhosphoSiteiQ9NRJ5.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

ArrayExpressiQ9NRJ5.
BgeeiQ9NRJ5.
CleanExiHS_PAPOLB.
GenevestigatoriQ9NRJ5.

Organism-specific databases

HPAiHPA054427.

Interactioni

Subunit structurei

Interacts with GSG1 By similarity.

Protein-protein interaction databases

BioGridi121233. 1 interaction.
STRINGi9606.ENSP00000384700.

Structurei

3D structure databases

ProteinModelPortaliQ9NRJ5.
SMRiQ9NRJ5. Positions 20-498.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5186.
HOVERGENiHBG053502.
InParanoidiQ9NRJ5.
KOiK14376.
OMAiMSDGMAR.
OrthoDBiEOG7XH6PT.
PhylomeDBiQ9NRJ5.
TreeFamiTF300842.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NRJ5-1 [UniParc]FASTAAdd to Basket

« Hide

MPFPVTTQGP PQPAPPPNRY GVSSPISLAV PKETDCLLTQ RLIETLRPFG    50
VFEEEEELQR RILVLEKLNN LVKEWIREIS ESKSLPQSVI ENVGGKIFTF 100
GSYRLGVHTK GADIDALCVA PSHVDRSDFF TSFYAKLKLQ EEVKDLRAVE 150
EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS 200
LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA 250
MLVARTCQLY PNAVASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW 300
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS ISTRMVMIEE FKQGLAITHE 350
ILLSKAEWSK LFEAPSFFQK YKHYIVLLAS ASTEKQHLEW VGLVESKIRI 400
LVGSLEKNEF ITLAHVNPQS FPAPKENPDM EEFRTMWVIG LGLKKPDNSE 450
ILSIDLTYDI QSFTDTVYRQ AVNSKMFEMG MKITAMHLRR KELHQLLPHH 500
VLQDKKAHST EGRRLTDLND SSFDLSAGCE NSMSVPSSTS TMKTGPLISS 550
SQGRNSPALA VMTASVANIQ ATEFSLQQVN TNESSGVALN ESIPHAVSQP 600
AISPSPKAMV ARVVSSTCLI SHPDLQETQQ QTYLIL 636
Length:636
Mass (Da):71,681
Last modified:October 1, 2000 - v1
Checksum:i3A0823F4309C35DD
GO

Sequence cautioni

The sequence AAH36653.2 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661E → D in AAH36653. 1 Publication
Sequence conflicti150 – 1501E → Q in AAH36653. 1 Publication
Sequence conflicti486 – 4861M → V in AAH36653. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218840 mRNA. Translation: AAF81013.1.
AC092610 Genomic DNA. Translation: AAS07561.1.
BC036653 mRNA. Translation: AAH36653.2. Different initiation.
RefSeqiNP_064529.4. NM_020144.4.
UniGeneiHs.487409.

Genome annotation databases

EnsembliENST00000404991; ENSP00000384700; ENSG00000218823.
GeneIDi56903.
KEGGihsa:56903.

Polymorphism databases

DMDMi18203318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF218840 mRNA. Translation: AAF81013.1 .
AC092610 Genomic DNA. Translation: AAS07561.1 .
BC036653 mRNA. Translation: AAH36653.2 . Different initiation.
RefSeqi NP_064529.4. NM_020144.4.
UniGenei Hs.487409.

3D structure databases

ProteinModelPortali Q9NRJ5.
SMRi Q9NRJ5. Positions 20-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121233. 1 interaction.
STRINGi 9606.ENSP00000384700.

PTM databases

PhosphoSitei Q9NRJ5.

Polymorphism databases

DMDMi 18203318.

Proteomic databases

PaxDbi Q9NRJ5.
PRIDEi Q9NRJ5.

Protocols and materials databases

DNASUi 56903.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000404991 ; ENSP00000384700 ; ENSG00000218823 .
GeneIDi 56903.
KEGGi hsa:56903.

Organism-specific databases

CTDi 56903.
GeneCardsi GC07M004897.
HGNCi HGNC:15970. PAPOLB.
HPAi HPA054427.
MIMi 607436. gene.
neXtProti NX_Q9NRJ5.
PharmGKBi PA32933.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5186.
HOVERGENi HBG053502.
InParanoidi Q9NRJ5.
KOi K14376.
OMAi MSDGMAR.
OrthoDBi EOG7XH6PT.
PhylomeDBi Q9NRJ5.
TreeFami TF300842.

Miscellaneous databases

GenomeRNAii 56903.
NextBioi 62363.
PROi Q9NRJ5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9NRJ5.
Bgeei Q9NRJ5.
CleanExi HS_PAPOLB.
Genevestigatori Q9NRJ5.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Testis-specific poly(A) polymerase gene is conserved in human and mouse."
    Lee Y., Kim H., Chung J., Lee Y.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiPAPOB_HUMAN
AccessioniPrimary (citable) accession number: Q9NRJ5
Secondary accession number(s): Q75LH1, Q8NE14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi