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Q9NRJ5

- PAPOB_HUMAN

UniProt

Q9NRJ5 - PAPOB_HUMAN

Protein

Poly(A) polymerase beta

Gene

PAPOLB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091ATPBy similarity
    Metal bindingi113 – 1131Magnesium 1; catalyticBy similarity
    Metal bindingi113 – 1131Magnesium 2; catalyticBy similarity
    Metal bindingi115 – 1151Magnesium 1; catalyticBy similarity
    Metal bindingi115 – 1151Magnesium 2; catalyticBy similarity
    Sitei153 – 1531Interaction with RNABy similarity
    Sitei158 – 1581Interaction with RNABy similarity
    Metal bindingi167 – 1671Magnesium 2; catalyticBy similarity
    Binding sitei167 – 1671ATPBy similarity
    Binding sitei228 – 2281ATPBy similarity
    Binding sitei237 – 2371ATPBy similarity
    Sitei328 – 3281Interaction with RNABy similarity
    Sitei399 – 3991Interaction with RNABy similarity
    Sitei524 – 5241Interaction with RNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi100 – 1023ATPBy similarity
    Nucleotide bindingi113 – 1153ATPBy similarity
    Nucleotide bindingi246 – 2472ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: RefGenome
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA polyadenylation Source: RefGenome
    2. mRNA splicing, via spliceosome Source: RefGenome
    3. termination of RNA polymerase II transcription Source: RefGenome

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase beta (EC:2.7.7.19)
    Short name:
    PAP-beta
    Alternative name(s):
    Polynucleotide adenylyltransferase beta
    Testis-specific poly(A) polymerase
    Gene namesi
    Name:PAPOLB
    Synonyms:PAPT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:15970. PAPOLB.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. endoplasmic reticulum Source: Ensembl
    3. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA32933.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 636636Poly(A) polymerase betaPRO_0000051622Add
    BLAST

    Proteomic databases

    PaxDbiQ9NRJ5.
    PRIDEiQ9NRJ5.

    PTM databases

    PhosphoSiteiQ9NRJ5.

    Expressioni

    Tissue specificityi

    Testis specific.

    Gene expression databases

    ArrayExpressiQ9NRJ5.
    BgeeiQ9NRJ5.
    CleanExiHS_PAPOLB.
    GenevestigatoriQ9NRJ5.

    Organism-specific databases

    HPAiHPA054427.

    Interactioni

    Subunit structurei

    Interacts with GSG1.By similarity

    Protein-protein interaction databases

    BioGridi121233. 1 interaction.
    STRINGi9606.ENSP00000384700.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NRJ5.
    SMRiQ9NRJ5. Positions 20-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiCOG5186.
    HOVERGENiHBG053502.
    InParanoidiQ9NRJ5.
    KOiK14376.
    OMAiMSDGMAR.
    OrthoDBiEOG7XH6PT.
    PhylomeDBiQ9NRJ5.
    TreeFamiTF300842.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9NRJ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPFPVTTQGP PQPAPPPNRY GVSSPISLAV PKETDCLLTQ RLIETLRPFG    50
    VFEEEEELQR RILVLEKLNN LVKEWIREIS ESKSLPQSVI ENVGGKIFTF 100
    GSYRLGVHTK GADIDALCVA PSHVDRSDFF TSFYAKLKLQ EEVKDLRAVE 150
    EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS 200
    LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA 250
    MLVARTCQLY PNAVASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW 300
    DPRVNPSDRY HLMPIITPAY PQQNSTYNVS ISTRMVMIEE FKQGLAITHE 350
    ILLSKAEWSK LFEAPSFFQK YKHYIVLLAS ASTEKQHLEW VGLVESKIRI 400
    LVGSLEKNEF ITLAHVNPQS FPAPKENPDM EEFRTMWVIG LGLKKPDNSE 450
    ILSIDLTYDI QSFTDTVYRQ AVNSKMFEMG MKITAMHLRR KELHQLLPHH 500
    VLQDKKAHST EGRRLTDLND SSFDLSAGCE NSMSVPSSTS TMKTGPLISS 550
    SQGRNSPALA VMTASVANIQ ATEFSLQQVN TNESSGVALN ESIPHAVSQP 600
    AISPSPKAMV ARVVSSTCLI SHPDLQETQQ QTYLIL 636
    Length:636
    Mass (Da):71,681
    Last modified:October 1, 2000 - v1
    Checksum:i3A0823F4309C35DD
    GO

    Sequence cautioni

    The sequence AAH36653.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661E → D in AAH36653. (PubMed:15489334)Curated
    Sequence conflicti150 – 1501E → Q in AAH36653. (PubMed:15489334)Curated
    Sequence conflicti486 – 4861M → V in AAH36653. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF218840 mRNA. Translation: AAF81013.1.
    AC092610 Genomic DNA. Translation: AAS07561.1.
    BC036653 mRNA. Translation: AAH36653.2. Different initiation.
    RefSeqiNP_064529.4. NM_020144.4.
    UniGeneiHs.487409.

    Genome annotation databases

    EnsembliENST00000404991; ENSP00000384700; ENSG00000218823.
    GeneIDi56903.
    KEGGihsa:56903.

    Polymorphism databases

    DMDMi18203318.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF218840 mRNA. Translation: AAF81013.1 .
    AC092610 Genomic DNA. Translation: AAS07561.1 .
    BC036653 mRNA. Translation: AAH36653.2 . Different initiation.
    RefSeqi NP_064529.4. NM_020144.4.
    UniGenei Hs.487409.

    3D structure databases

    ProteinModelPortali Q9NRJ5.
    SMRi Q9NRJ5. Positions 20-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121233. 1 interaction.
    STRINGi 9606.ENSP00000384700.

    PTM databases

    PhosphoSitei Q9NRJ5.

    Polymorphism databases

    DMDMi 18203318.

    Proteomic databases

    PaxDbi Q9NRJ5.
    PRIDEi Q9NRJ5.

    Protocols and materials databases

    DNASUi 56903.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000404991 ; ENSP00000384700 ; ENSG00000218823 .
    GeneIDi 56903.
    KEGGi hsa:56903.

    Organism-specific databases

    CTDi 56903.
    GeneCardsi GC07M004897.
    HGNCi HGNC:15970. PAPOLB.
    HPAi HPA054427.
    MIMi 607436. gene.
    neXtProti NX_Q9NRJ5.
    PharmGKBi PA32933.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5186.
    HOVERGENi HBG053502.
    InParanoidi Q9NRJ5.
    KOi K14376.
    OMAi MSDGMAR.
    OrthoDBi EOG7XH6PT.
    PhylomeDBi Q9NRJ5.
    TreeFami TF300842.

    Miscellaneous databases

    GenomeRNAii 56903.
    NextBioi 62363.
    PROi Q9NRJ5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NRJ5.
    Bgeei Q9NRJ5.
    CleanExi HS_PAPOLB.
    Genevestigatori Q9NRJ5.

    Family and domain databases

    Gene3Di 3.30.70.590. 1 hit.
    InterProi IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMi SSF55003. SSF55003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Testis-specific poly(A) polymerase gene is conserved in human and mouse."
      Lee Y., Kim H., Chung J., Lee Y.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiPAPOB_HUMAN
    AccessioniPrimary (citable) accession number: Q9NRJ5
    Secondary accession number(s): Q75LH1, Q8NE14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3