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Q9NRJ5

- PAPOB_HUMAN

UniProt

Q9NRJ5 - PAPOB_HUMAN

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Protein

Poly(A) polymerase beta

Gene

PAPOLB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATPBy similarity
Metal bindingi113 – 1131Magnesium 1; catalyticBy similarity
Metal bindingi113 – 1131Magnesium 2; catalyticBy similarity
Metal bindingi115 – 1151Magnesium 1; catalyticBy similarity
Metal bindingi115 – 1151Magnesium 2; catalyticBy similarity
Sitei153 – 1531Interaction with RNABy similarity
Sitei158 – 1581Interaction with RNABy similarity
Metal bindingi167 – 1671Magnesium 2; catalyticBy similarity
Binding sitei167 – 1671ATPBy similarity
Binding sitei228 – 2281ATPBy similarity
Binding sitei237 – 2371ATPBy similarity
Sitei328 – 3281Interaction with RNABy similarity
Sitei399 – 3991Interaction with RNABy similarity
Sitei524 – 5241Interaction with RNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1023ATPBy similarity
Nucleotide bindingi113 – 1153ATPBy similarity
Nucleotide bindingi246 – 2472ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: RefGenome
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: RefGenome
  2. mRNA splicing, via spliceosome Source: RefGenome
  3. termination of RNA polymerase II transcription Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase beta (EC:2.7.7.19)
Short name:
PAP-beta
Alternative name(s):
Polynucleotide adenylyltransferase beta
Testis-specific poly(A) polymerase
Gene namesi
Name:PAPOLB
Synonyms:PAPT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:15970. PAPOLB.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. endoplasmic reticulum Source: Ensembl
  3. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32933.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 636636Poly(A) polymerase betaPRO_0000051622Add
BLAST

Proteomic databases

PaxDbiQ9NRJ5.
PRIDEiQ9NRJ5.

PTM databases

PhosphoSiteiQ9NRJ5.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiQ9NRJ5.
CleanExiHS_PAPOLB.
ExpressionAtlasiQ9NRJ5. baseline and differential.
GenevestigatoriQ9NRJ5.

Organism-specific databases

HPAiHPA054427.

Interactioni

Subunit structurei

Interacts with GSG1.By similarity

Protein-protein interaction databases

BioGridi121233. 1 interaction.
STRINGi9606.ENSP00000384700.

Structurei

3D structure databases

ProteinModelPortaliQ9NRJ5.
SMRiQ9NRJ5. Positions 20-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiCOG5186.
HOVERGENiHBG053502.
InParanoidiQ9NRJ5.
KOiK14376.
OMAiMSDGMAR.
OrthoDBiEOG7XH6PT.
PhylomeDBiQ9NRJ5.
TreeFamiTF300842.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NRJ5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPFPVTTQGP PQPAPPPNRY GVSSPISLAV PKETDCLLTQ RLIETLRPFG
60 70 80 90 100
VFEEEEELQR RILVLEKLNN LVKEWIREIS ESKSLPQSVI ENVGGKIFTF
110 120 130 140 150
GSYRLGVHTK GADIDALCVA PSHVDRSDFF TSFYAKLKLQ EEVKDLRAVE
160 170 180 190 200
EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS
210 220 230 240 250
LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA
260 270 280 290 300
MLVARTCQLY PNAVASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW
310 320 330 340 350
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS ISTRMVMIEE FKQGLAITHE
360 370 380 390 400
ILLSKAEWSK LFEAPSFFQK YKHYIVLLAS ASTEKQHLEW VGLVESKIRI
410 420 430 440 450
LVGSLEKNEF ITLAHVNPQS FPAPKENPDM EEFRTMWVIG LGLKKPDNSE
460 470 480 490 500
ILSIDLTYDI QSFTDTVYRQ AVNSKMFEMG MKITAMHLRR KELHQLLPHH
510 520 530 540 550
VLQDKKAHST EGRRLTDLND SSFDLSAGCE NSMSVPSSTS TMKTGPLISS
560 570 580 590 600
SQGRNSPALA VMTASVANIQ ATEFSLQQVN TNESSGVALN ESIPHAVSQP
610 620 630
AISPSPKAMV ARVVSSTCLI SHPDLQETQQ QTYLIL
Length:636
Mass (Da):71,681
Last modified:October 1, 2000 - v1
Checksum:i3A0823F4309C35DD
GO

Sequence cautioni

The sequence AAH36653.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661E → D in AAH36653. (PubMed:15489334)Curated
Sequence conflicti150 – 1501E → Q in AAH36653. (PubMed:15489334)Curated
Sequence conflicti486 – 4861M → V in AAH36653. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218840 mRNA. Translation: AAF81013.1.
AC092610 Genomic DNA. Translation: AAS07561.1.
BC036653 mRNA. Translation: AAH36653.2. Different initiation.
RefSeqiNP_064529.4. NM_020144.4.
UniGeneiHs.487409.

Genome annotation databases

EnsembliENST00000404991; ENSP00000384700; ENSG00000218823.
GeneIDi56903.
KEGGihsa:56903.

Polymorphism databases

DMDMi18203318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218840 mRNA. Translation: AAF81013.1 .
AC092610 Genomic DNA. Translation: AAS07561.1 .
BC036653 mRNA. Translation: AAH36653.2 . Different initiation.
RefSeqi NP_064529.4. NM_020144.4.
UniGenei Hs.487409.

3D structure databases

ProteinModelPortali Q9NRJ5.
SMRi Q9NRJ5. Positions 20-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121233. 1 interaction.
STRINGi 9606.ENSP00000384700.

PTM databases

PhosphoSitei Q9NRJ5.

Polymorphism databases

DMDMi 18203318.

Proteomic databases

PaxDbi Q9NRJ5.
PRIDEi Q9NRJ5.

Protocols and materials databases

DNASUi 56903.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000404991 ; ENSP00000384700 ; ENSG00000218823 .
GeneIDi 56903.
KEGGi hsa:56903.

Organism-specific databases

CTDi 56903.
GeneCardsi GC07M004897.
HGNCi HGNC:15970. PAPOLB.
HPAi HPA054427.
MIMi 607436. gene.
neXtProti NX_Q9NRJ5.
PharmGKBi PA32933.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5186.
HOVERGENi HBG053502.
InParanoidi Q9NRJ5.
KOi K14376.
OMAi MSDGMAR.
OrthoDBi EOG7XH6PT.
PhylomeDBi Q9NRJ5.
TreeFami TF300842.

Miscellaneous databases

GenomeRNAii 56903.
NextBioi 62363.
PROi Q9NRJ5.
SOURCEi Search...

Gene expression databases

Bgeei Q9NRJ5.
CleanExi HS_PAPOLB.
ExpressionAtlasi Q9NRJ5. baseline and differential.
Genevestigatori Q9NRJ5.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Testis-specific poly(A) polymerase gene is conserved in human and mouse."
    Lee Y., Kim H., Chung J., Lee Y.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiPAPOB_HUMAN
AccessioniPrimary (citable) accession number: Q9NRJ5
Secondary accession number(s): Q75LH1, Q8NE14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3