ID   CCL28_HUMAN             Reviewed;         127 AA.
AC   Q9NRJ3; D7RIE7;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 186.
DE   RecName: Full=C-C motif chemokine 28;
DE   AltName: Full=Mucosae-associated epithelial chemokine;
DE            Short=MEC;
DE   AltName: Full=Protein CCK1;
DE   AltName: Full=Small-inducible cytokine A28;
DE   Flags: Precursor;
GN   Name=CCL28; Synonyms=SCYA28;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND RECEPTOR INTERACTION.
RC   TISSUE=Fetal heart, and Osteoblast;
RX   PubMed=10781587; DOI=10.1074/jbc.m001461200;
RA   Wang W., Soto H., Oldham E.R., Buchanan M.E., Homey B., Catron D.,
RA   Jenkins N., Copeland N.G., Gilbert D.J., Nguyen N., Abrams J.,
RA   Kershenovich D., Smith K., McClanahan T., Vicari A.P., Zlotnik A.;
RT   "Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2).";
RL   J. Biol. Chem. 275:22313-22323(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND RECEPTOR INTERACTION.
RX   PubMed=10975800; DOI=10.4049/jimmunol.165.6.2943;
RA   Pan J., Kunkel E.J., Gosslar U., Lazarus N., Langdon P., Broadwell K.,
RA   Vierra M.A., Genovese M.C., Butcher E.C., Soler D.;
RT   "A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells
RT   in mucosal tissues.";
RL   J. Immunol. 165:2943-2949(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA   Zhang W., He L., Yuan Z., Wan T., Cao X.;
RT   "A novel CC chemokine homology with TECK.";
RL   Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND ALTERNATIVE SPLICING.
RA   Scanlon K.M., Mahon B.P.;
RT   "Prediction and identification of a novel CCL28 splice variant CCL28chi.";
RL   Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15372022; DOI=10.1038/nature02919;
RA   Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA   Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA   She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA   Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA   Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA   Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA   Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA   Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA   Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA   Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA   Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA   Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA   Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT   "The DNA sequence and comparative analysis of human chromosome 5.";
RL   Nature 431:268-274(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Chemotactic activity for resting CD4, CD8 T-cells and
CC       eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization
CC       in a dose-dependent manner.
CC   -!- INTERACTION:
CC       Q9NRJ3; P51671: CCL11; NbExp=2; IntAct=EBI-7783254, EBI-727357;
CC       Q9NRJ3; Q92583: CCL17; NbExp=3; IntAct=EBI-7783254, EBI-16640146;
CC       Q9NRJ3; O00585: CCL21; NbExp=2; IntAct=EBI-7783254, EBI-953695;
CC       Q9NRJ3; Q9Y258: CCL26; NbExp=2; IntAct=EBI-7783254, EBI-7783416;
CC       Q9NRJ3; P13501: CCL5; NbExp=2; IntAct=EBI-7783254, EBI-2848366;
CC       Q9NRJ3; P02778: CXCL10; NbExp=2; IntAct=EBI-7783254, EBI-7815386;
CC       Q9NRJ3; P48061: CXCL12; NbExp=2; IntAct=EBI-7783254, EBI-3913254;
CC       Q9NRJ3; Q8WTU0: DDI1; NbExp=10; IntAct=EBI-7783254, EBI-748248;
CC       Q9NRJ3; P51808: DYNLT3; NbExp=3; IntAct=EBI-7783254, EBI-743027;
CC       Q9NRJ3; P02776: PF4; NbExp=3; IntAct=EBI-7783254, EBI-2565740;
CC       Q9NRJ3; P25788: PSMA3; NbExp=3; IntAct=EBI-7783254, EBI-348380;
CC       Q9NRJ3; Q9UNE7: STUB1; NbExp=3; IntAct=EBI-7783254, EBI-357085;
CC       Q9NRJ3; Q86WV8: TSC1; NbExp=3; IntAct=EBI-7783254, EBI-12806590;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9NRJ3-1; Sequence=Displayed;
CC       Name=2; Synonyms=CCL28chi;
CC         IsoId=Q9NRJ3-2; Sequence=VSP_047735;
CC   -!- TISSUE SPECIFICITY: Preferentially expressed by epithelial cells of
CC       diverse tissues including normal and pathological colon, salivary
CC       gland, mammary gland, trachea and rectum. Also found in prostate,
CC       spleen, thyroid, psoriasis skin and in lower levels in peripheral blood
CC       leukocytes, small intestine, Peyer patches, stomach and normal skin.
CC   -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Wikipedia; Note=CCL28 entry;
CC       URL="https://en.wikipedia.org/wiki/CCL28";
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DR   EMBL; AF220210; AAF87205.1; -; mRNA.
DR   EMBL; AF266504; AAG16691.1; -; mRNA.
DR   EMBL; AF110384; AAG43193.1; -; mRNA.
DR   EMBL; HM067700; ADH95184.1; -; mRNA.
DR   EMBL; AC025457; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC062668; AAH62668.1; -; mRNA.
DR   EMBL; BC069532; AAH69532.1; -; mRNA.
DR   CCDS; CCDS3944.1; -. [Q9NRJ3-1]
DR   RefSeq; NP_001288802.1; NM_001301873.1. [Q9NRJ3-1]
DR   RefSeq; NP_001288803.1; NM_001301874.1. [Q9NRJ3-1]
DR   RefSeq; NP_683513.1; NM_148672.3. [Q9NRJ3-1]
DR   PDB; 6CWS; NMR; -; A=20-127.
DR   PDBsum; 6CWS; -.
DR   AlphaFoldDB; Q9NRJ3; -.
DR   BMRB; Q9NRJ3; -.
DR   SMR; Q9NRJ3; -.
DR   BioGRID; 121147; 23.
DR   DIP; DIP-57392N; -.
DR   IntAct; Q9NRJ3; 26.
DR   MINT; Q9NRJ3; -.
DR   STRING; 9606.ENSP00000354416; -.
DR   GlyCosmos; Q9NRJ3; 1 site, No reported glycans.
DR   GlyGen; Q9NRJ3; 1 site.
DR   iPTMnet; Q9NRJ3; -.
DR   PhosphoSitePlus; Q9NRJ3; -.
DR   BioMuta; CCL28; -.
DR   DMDM; 12230650; -.
DR   jPOST; Q9NRJ3; -.
DR   MassIVE; Q9NRJ3; -.
DR   PaxDb; 9606-ENSP00000354416; -.
DR   PeptideAtlas; Q9NRJ3; -.
DR   ProteomicsDB; 15169; -.
DR   ProteomicsDB; 82377; -. [Q9NRJ3-1]
DR   Pumba; Q9NRJ3; -.
DR   Antibodypedia; 23245; 387 antibodies from 31 providers.
DR   DNASU; 56477; -.
DR   Ensembl; ENST00000361115.4; ENSP00000354416.4; ENSG00000151882.11. [Q9NRJ3-1]
DR   Ensembl; ENST00000489442.5; ENSP00000426424.1; ENSG00000151882.11. [Q9NRJ3-1]
DR   GeneID; 56477; -.
DR   KEGG; hsa:56477; -.
DR   MANE-Select; ENST00000361115.4; ENSP00000354416.4; NM_148672.3; NP_683513.1.
DR   UCSC; uc003jnu.4; human. [Q9NRJ3-1]
DR   AGR; HGNC:17700; -.
DR   CTD; 56477; -.
DR   DisGeNET; 56477; -.
DR   GeneCards; CCL28; -.
DR   HGNC; HGNC:17700; CCL28.
DR   HPA; ENSG00000151882; Tissue enriched (salivary).
DR   MIM; 605240; gene.
DR   neXtProt; NX_Q9NRJ3; -.
DR   OpenTargets; ENSG00000151882; -.
DR   PharmGKB; PA38463; -.
DR   VEuPathDB; HostDB:ENSG00000151882; -.
DR   eggNOG; ENOG502SVUE; Eukaryota.
DR   GeneTree; ENSGT00530000063923; -.
DR   HOGENOM; CLU_2003113_0_0_1; -.
DR   InParanoid; Q9NRJ3; -.
DR   OMA; ICVSPHS; -.
DR   OrthoDB; 5323694at2759; -.
DR   PhylomeDB; Q9NRJ3; -.
DR   TreeFam; TF337014; -.
DR   PathwayCommons; Q9NRJ3; -.
DR   Reactome; R-HSA-380108; Chemokine receptors bind chemokines.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   SignaLink; Q9NRJ3; -.
DR   BioGRID-ORCS; 56477; 13 hits in 1147 CRISPR screens.
DR   ChiTaRS; CCL28; human.
DR   GenomeRNAi; 56477; -.
DR   Pharos; Q9NRJ3; Tbio.
DR   PRO; PR:Q9NRJ3; -.
DR   Proteomes; UP000005640; Chromosome 5.
DR   RNAct; Q9NRJ3; Protein.
DR   Bgee; ENSG00000151882; Expressed in parotid gland and 138 other cell types or tissues.
DR   ExpressionAtlas; Q9NRJ3; baseline and differential.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; IDA:UniProtKB.
DR   GO; GO:0060326; P:cell chemotaxis; IDA:UniProtKB.
DR   GO; GO:0006935; P:chemotaxis; TAS:UniProtKB.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   GO; GO:1903237; P:negative regulation of leukocyte tethering or rolling; IDA:UniProtKB.
DR   GO; GO:0001954; P:positive regulation of cell-matrix adhesion; IDA:UniProtKB.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IEA:Ensembl.
DR   GO; GO:0007584; P:response to nutrient; IEA:Ensembl.
DR   DisProt; DP02010; -.
DR   Gene3D; 2.40.50.40; -; 1.
DR   InterPro; IPR000827; Chemokine_CC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   Pfam; PF00048; IL8; 1.
DR   SUPFAM; SSF54117; Interleukin 8-like chemokines; 1.
DR   PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
DR   Genevisible; Q9NRJ3; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Chemotaxis; Cytokine; Disulfide bond;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..127
FT                   /note="C-C motif chemokine 28"
FT                   /id="PRO_0000005242"
FT   REGION          92..127
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        95..112
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        113..127
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        78
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        30..58
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..73
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         64..127
FT                   /note="ILHVKRRRICVSPHNHTVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQ
FT                   GKHETYGHKTPY -> MLECSDMISAHYNLHLPGSNDSPTSAFQVAGITAFMSSAEESV
FT                   SARTTILLSSG (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.4"
FT                   /id="VSP_047735"
FT   TURN            22..25
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   STRAND          27..30
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   HELIX           40..43
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   STRAND          46..52
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   TURN            54..57
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   STRAND          62..69
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   STRAND          71..74
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   HELIX           79..89
FT                   /evidence="ECO:0007829|PDB:6CWS"
FT   STRAND          103..106
FT                   /evidence="ECO:0007829|PDB:6CWS"
SQ   SEQUENCE   127 AA;  14280 MW;  3E8551A63A2C8D62 CRC64;
     MQQRGLAIVA LAVCAALHAS EAILPIASSC CTEVSHHISR RLLERVNMCR IQRADGDCDL
     AAVILHVKRR RICVSPHNHT VKQWMKVQAA KKNGKGNVCH RKKHHGKRNS NRAHQGKHET
     YGHKTPY
//