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Protein

C-C motif chemokine 28

Gene

CCL28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.

GO - Molecular functioni

  1. chemokine activity Source: UniProtKB

GO - Biological processi

  1. cell chemotaxis Source: UniProtKB
  2. chemotaxis Source: UniProtKB
  3. immune response Source: InterPro
  4. negative regulation of leukocyte tethering or rolling Source: UniProtKB
  5. positive regulation of cell-matrix adhesion Source: UniProtKB
  6. positive regulation of cytosolic calcium ion concentration Source: Ensembl
  7. response to nutrient Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiREACT_15344. Chemokine receptors bind chemokines.
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 28
Alternative name(s):
Mucosae-associated epithelial chemokine
Short name:
MEC
Protein CCK1
Small-inducible cytokine A28
Gene namesi
Name:CCL28
Synonyms:SCYA28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:17700. CCL28.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38463.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 127108C-C motif chemokine 28PRO_0000005242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 58By similarity
Disulfide bondi31 ↔ 73By similarity
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NRJ3.
PRIDEiQ9NRJ3.

PTM databases

PhosphoSiteiQ9NRJ3.

Expressioni

Tissue specificityi

Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin.

Gene expression databases

BgeeiQ9NRJ3.
CleanExiHS_CCL28.
ExpressionAtlasiQ9NRJ3. baseline and differential.
GenevestigatoriQ9NRJ3.

Interactioni

Protein-protein interaction databases

BioGridi121147. 3 interactions.
DIPiDIP-57392N.
IntActiQ9NRJ3. 2 interactions.
MINTiMINT-6629016.
STRINGi9606.ENSP00000354416.

Structurei

3D structure databases

ProteinModelPortaliQ9NRJ3.
SMRiQ9NRJ3. Positions 23-94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG41057.
GeneTreeiENSGT00530000063923.
HOGENOMiHOG000036970.
HOVERGENiHBG101413.
InParanoidiQ9NRJ3.
KOiK05513.
OMAiGNICHKK.
OrthoDBiEOG70W3G4.
PhylomeDBiQ9NRJ3.
TreeFamiTF337014.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NRJ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQRGLAIVA LAVCAALHAS EAILPIASSC CTEVSHHISR RLLERVNMCR
60 70 80 90 100
IQRADGDCDL AAVILHVKRR RICVSPHNHT VKQWMKVQAA KKNGKGNVCH
110 120
RKKHHGKRNS NRAHQGKHET YGHKTPY
Length:127
Mass (Da):14,280
Last modified:October 1, 2000 - v1
Checksum:i3E8551A63A2C8D62
GO
Isoform 2 (identifier: Q9NRJ3-2) [UniParc]FASTAAdd to basket

Also known as: CCL28chi

The sequence of this isoform differs from the canonical sequence as follows:
     64-127: ILHVKRRRIC...HETYGHKTPY → MLECSDMISA...ARTTILLSSG

Show »
Length:117
Mass (Da):12,375
Checksum:i2E0390498F47B135
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei64 – 12764ILHVK…HKTPY → MLECSDMISAHYNLHLPGSN DSPTSAFQVAGITAFMSSAE ESVSARTTILLSSG in isoform 2. 1 PublicationVSP_047735Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220210 mRNA. Translation: AAF87205.1.
AF266504 mRNA. Translation: AAG16691.1.
AF110384 mRNA. Translation: AAG43193.1.
HM067700 mRNA. Translation: ADH95184.1.
AC025457 Genomic DNA. No translation available.
BC062668 mRNA. Translation: AAH62668.1.
BC069532 mRNA. Translation: AAH69532.1.
CCDSiCCDS3944.1. [Q9NRJ3-1]
RefSeqiNP_001288802.1. NM_001301873.1. [Q9NRJ3-1]
NP_001288803.1. NM_001301874.1. [Q9NRJ3-1]
NP_683513.1. NM_148672.3. [Q9NRJ3-1]
XP_005248382.1. XM_005248325.1. [Q9NRJ3-2]
UniGeneiHs.656904.

Genome annotation databases

EnsembliENST00000361115; ENSP00000354416; ENSG00000151882. [Q9NRJ3-1]
ENST00000489442; ENSP00000426424; ENSG00000151882. [Q9NRJ3-1]
GeneIDi56477.
KEGGihsa:56477.
UCSCiuc003jnu.3. human. [Q9NRJ3-1]

Polymorphism databases

DMDMi12230650.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Wikipedia

CCL28 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220210 mRNA. Translation: AAF87205.1.
AF266504 mRNA. Translation: AAG16691.1.
AF110384 mRNA. Translation: AAG43193.1.
HM067700 mRNA. Translation: ADH95184.1.
AC025457 Genomic DNA. No translation available.
BC062668 mRNA. Translation: AAH62668.1.
BC069532 mRNA. Translation: AAH69532.1.
CCDSiCCDS3944.1. [Q9NRJ3-1]
RefSeqiNP_001288802.1. NM_001301873.1. [Q9NRJ3-1]
NP_001288803.1. NM_001301874.1. [Q9NRJ3-1]
NP_683513.1. NM_148672.3. [Q9NRJ3-1]
XP_005248382.1. XM_005248325.1. [Q9NRJ3-2]
UniGeneiHs.656904.

3D structure databases

ProteinModelPortaliQ9NRJ3.
SMRiQ9NRJ3. Positions 23-94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121147. 3 interactions.
DIPiDIP-57392N.
IntActiQ9NRJ3. 2 interactions.
MINTiMINT-6629016.
STRINGi9606.ENSP00000354416.

PTM databases

PhosphoSiteiQ9NRJ3.

Polymorphism databases

DMDMi12230650.

Proteomic databases

PaxDbiQ9NRJ3.
PRIDEiQ9NRJ3.

Protocols and materials databases

DNASUi56477.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361115; ENSP00000354416; ENSG00000151882. [Q9NRJ3-1]
ENST00000489442; ENSP00000426424; ENSG00000151882. [Q9NRJ3-1]
GeneIDi56477.
KEGGihsa:56477.
UCSCiuc003jnu.3. human. [Q9NRJ3-1]

Organism-specific databases

CTDi56477.
GeneCardsiGC05M043376.
HGNCiHGNC:17700. CCL28.
MIMi605240. gene.
neXtProtiNX_Q9NRJ3.
PharmGKBiPA38463.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG41057.
GeneTreeiENSGT00530000063923.
HOGENOMiHOG000036970.
HOVERGENiHBG101413.
InParanoidiQ9NRJ3.
KOiK05513.
OMAiGNICHKK.
OrthoDBiEOG70W3G4.
PhylomeDBiQ9NRJ3.
TreeFamiTF337014.

Enzyme and pathway databases

ReactomeiREACT_15344. Chemokine receptors bind chemokines.
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiCCL28. human.
GenomeRNAii56477.
NextBioi35495724.
PROiQ9NRJ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NRJ3.
CleanExiHS_CCL28.
ExpressionAtlasiQ9NRJ3. baseline and differential.
GenevestigatoriQ9NRJ3.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), RECEPTOR INTERACTION.
    Tissue: Fetal heart and Osteoblast.
  2. "A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues."
    Pan J., Kunkel E.J., Gosslar U., Lazarus N., Langdon P., Broadwell K., Vierra M.A., Genovese M.C., Butcher E.C., Soler D.
    J. Immunol. 165:2943-2949(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), RECEPTOR INTERACTION.
  3. "A novel CC chemokine homology with TECK."
    Zhang W., He L., Yuan Z., Wan T., Cao X.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Prediction and identification of a novel CCL28 splice variant CCL28chi."
    Scanlon K.M., Mahon B.P.
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
  5. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiCCL28_HUMAN
AccessioniPrimary (citable) accession number: Q9NRJ3
Secondary accession number(s): D7RIE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.