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Protein

DNA polymerase epsilon subunit 3

Gene

POLE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: ProtInc

GO - Biological processi

  • DNA replication Source: ProtInc
  • histone H3 acetylation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS07503-MONOMER.
ReactomeiR-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-174430. Telomere C-strand synthesis initiation.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-68952. DNA replication initiation.
R-HSA-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 3 (EC:2.7.7.7)
Alternative name(s):
Arsenic-transactivated protein
Short name:
AsTP
Chromatin accessibility complex 17 kDa protein
Short name:
CHRAC-17
Short name:
HuCHRAC17
DNA polymerase II subunit 3
DNA polymerase epsilon subunit p17
Gene namesi
Name:POLE3
Synonyms:CHRAC17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:13546. POLE3.

Subcellular locationi

GO - Cellular componenti

  • Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
  • epsilon DNA polymerase complex Source: UniProtKB
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi54107.
OpenTargetsiENSG00000148229.
PharmGKBiPA33499.

Chemistry databases

DrugBankiDB00242. Cladribine.

Polymorphism and mutation databases

BioMutaiPOLE3.
DMDMi22653710.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002083412 – 147DNA polymerase epsilon subunit 3Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei83PhosphothreonineCombined sources1
Modified residuei122PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NRF9.
MaxQBiQ9NRF9.
PaxDbiQ9NRF9.
PeptideAtlasiQ9NRF9.
PRIDEiQ9NRF9.
TopDownProteomicsiQ9NRF9.

PTM databases

iPTMnetiQ9NRF9.
PhosphoSitePlusiQ9NRF9.
SwissPalmiQ9NRF9.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Gene expression databases

BgeeiENSG00000148229.
CleanExiHS_POLE3.
ExpressionAtlasiQ9NRF9. baseline and differential.
GenevisibleiQ9NRF9. HS.

Organism-specific databases

HPAiHPA047945.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CHRAC1Q9NRG010EBI-744901,EBI-2795492
DKFZp666G145Q658N33EBI-744901,EBI-10240005
DRAP1Q149196EBI-744901,EBI-712941
ORFQ9Q2G45EBI-744901,EBI-6248094From a different organism.
POLE4Q9NR337EBI-744901,EBI-867034

Protein-protein interaction databases

BioGridi119903. 28 interactors.
IntActiQ9NRF9. 11 interactors.
MINTiMINT-1439872.
STRINGi9606.ENSP00000363284.

Structurei

3D structure databases

ProteinModelPortaliQ9NRF9.
SMRiQ9NRF9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 146Sequence analysisAdd BLAST62

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0870. Eukaryota.
ENOG4111QSW. LUCA.
GeneTreeiENSGT00550000074689.
HOGENOMiHOG000007701.
HOVERGENiHBG060262.
InParanoidiQ9NRF9.
KOiK02326.
OMAiNFAMKAK.
OrthoDBiEOG091G0Y4D.
PhylomeDBiQ9NRF9.
TreeFamiTF103008.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NRF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS
60 70 80 90 100
CANNFAMKGK RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK
110 120 130 140
EASEQKKKDK DKKTDSEEQD KSRDEDNDED EERLEEEEQN EEEEVDN
Length:147
Mass (Da):16,860
Last modified:October 1, 2000 - v1
Checksum:iDC0A4D2097046569
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58K → N in AAU15052 (Ref. 3) Curated1
Sequence conflicti58K → N in BAC11206 (PubMed:14702039).Curated1
Sequence conflicti83T → I in BAC11190 (PubMed:14702039).Curated1
Sequence conflicti146D → Y in AAF90133 (PubMed:10801849).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02346483T → A.1 PublicationCorresponds to variant rs36023979dbSNPEnsembl.1
Natural variantiVAR_057527126D → A.Corresponds to variant rs34852828dbSNPEnsembl.1
Natural variantiVAR_023465135E → D.1 PublicationCorresponds to variant rs35933626dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226077 mRNA. Translation: AAF72417.1.
AF261689 Genomic DNA. Translation: AAF90133.1.
AY720898 mRNA. Translation: AAU15052.1.
AK074629 mRNA. Translation: BAC11099.1.
AK074762 mRNA. Translation: BAC11190.1.
AK074782 mRNA. Translation: BAC11206.1.
DQ072116 Genomic DNA. Translation: AAY57326.1.
AL137066 Genomic DNA. Translation: CAH70100.1.
BC003166 mRNA. Translation: AAH03166.1.
BC004170 mRNA. Translation: AAH04170.1.
CCDSiCCDS6795.1.
RefSeqiNP_001265184.1. NM_001278255.1.
NP_059139.3. NM_017443.4.
UniGeneiHs.108112.

Genome annotation databases

EnsembliENST00000374169; ENSP00000363284; ENSG00000148229.
ENST00000374171; ENSP00000363286; ENSG00000148229.
GeneIDi54107.
KEGGihsa:54107.
UCSCiuc004bhn.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226077 mRNA. Translation: AAF72417.1.
AF261689 Genomic DNA. Translation: AAF90133.1.
AY720898 mRNA. Translation: AAU15052.1.
AK074629 mRNA. Translation: BAC11099.1.
AK074762 mRNA. Translation: BAC11190.1.
AK074782 mRNA. Translation: BAC11206.1.
DQ072116 Genomic DNA. Translation: AAY57326.1.
AL137066 Genomic DNA. Translation: CAH70100.1.
BC003166 mRNA. Translation: AAH03166.1.
BC004170 mRNA. Translation: AAH04170.1.
CCDSiCCDS6795.1.
RefSeqiNP_001265184.1. NM_001278255.1.
NP_059139.3. NM_017443.4.
UniGeneiHs.108112.

3D structure databases

ProteinModelPortaliQ9NRF9.
SMRiQ9NRF9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119903. 28 interactors.
IntActiQ9NRF9. 11 interactors.
MINTiMINT-1439872.
STRINGi9606.ENSP00000363284.

Chemistry databases

DrugBankiDB00242. Cladribine.

PTM databases

iPTMnetiQ9NRF9.
PhosphoSitePlusiQ9NRF9.
SwissPalmiQ9NRF9.

Polymorphism and mutation databases

BioMutaiPOLE3.
DMDMi22653710.

Proteomic databases

EPDiQ9NRF9.
MaxQBiQ9NRF9.
PaxDbiQ9NRF9.
PeptideAtlasiQ9NRF9.
PRIDEiQ9NRF9.
TopDownProteomicsiQ9NRF9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374169; ENSP00000363284; ENSG00000148229.
ENST00000374171; ENSP00000363286; ENSG00000148229.
GeneIDi54107.
KEGGihsa:54107.
UCSCiuc004bhn.4. human.

Organism-specific databases

CTDi54107.
DisGeNETi54107.
GeneCardsiPOLE3.
HGNCiHGNC:13546. POLE3.
HPAiHPA047945.
MIMi607267. gene.
neXtProtiNX_Q9NRF9.
OpenTargetsiENSG00000148229.
PharmGKBiPA33499.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0870. Eukaryota.
ENOG4111QSW. LUCA.
GeneTreeiENSGT00550000074689.
HOGENOMiHOG000007701.
HOVERGENiHBG060262.
InParanoidiQ9NRF9.
KOiK02326.
OMAiNFAMKAK.
OrthoDBiEOG091G0Y4D.
PhylomeDBiQ9NRF9.
TreeFamiTF103008.

Enzyme and pathway databases

BioCyciZFISH:HS07503-MONOMER.
ReactomeiR-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-174430. Telomere C-strand synthesis initiation.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-68952. DNA replication initiation.
R-HSA-68962. Activation of the pre-replicative complex.

Miscellaneous databases

ChiTaRSiPOLE3. human.
GeneWikiiPOLE3.
GenomeRNAii54107.
PROiQ9NRF9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148229.
CleanExiHS_POLE3.
ExpressionAtlasiQ9NRF9. baseline and differential.
GenevisibleiQ9NRF9. HS.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE3_HUMAN
AccessioniPrimary (citable) accession number: Q9NRF9
Secondary accession number(s): Q5W0U1
, Q8N758, Q8NCE5, Q9NR32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.