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Protein

CTP synthase 2

Gene

CTPS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.2 Publications

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase, CTP synthase 1 (CTPS1), CTP synthase, CTP synthase (CTPS2), CTP synthase 2 (CTPS2)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei399For GATase activityPROSITE-ProRule annotation1
Active sitei526For GATase activityPROSITE-ProRule annotation1
Active sitei528For GATase activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS00585-MONOMER.
ZFISH:HS00585-MONOMER.
BRENDAi6.3.4.2. 2681.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SIGNORiQ9NRF8.
UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthase 2 (EC:6.3.4.2)
Alternative name(s):
CTP synthetase 2
UTP--ammonia ligase 2
Gene namesi
Name:CTPS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:2520. CTPS2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000047230.
PharmGKBiPA27021.

Polymorphism and mutation databases

BioMutaiCTPS2.
DMDMi74752919.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470331 – 586CTP synthase 2Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei568PhosphoserineCombined sources1
Modified residuei571PhosphoserineCombined sources1
Modified residuei574PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NRF8.
MaxQBiQ9NRF8.
PaxDbiQ9NRF8.
PeptideAtlasiQ9NRF8.
PRIDEiQ9NRF8.

PTM databases

iPTMnetiQ9NRF8.
PhosphoSitePlusiQ9NRF8.

Expressioni

Gene expression databases

BgeeiENSG00000047230.
CleanExiHS_CTPS2.
ExpressionAtlasiQ9NRF8. baseline and differential.
GenevisibleiQ9NRF8. HS.

Organism-specific databases

HPAiHPA017437.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SPG21Q9NZD84EBI-740874,EBI-742688

Protein-protein interaction databases

BioGridi121144. 31 interactors.
IntActiQ9NRF8. 11 interactors.
MINTiMINT-1443035.
STRINGi9606.ENSP00000352222.

Structurei

Secondary structure

1586
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Beta strandi10 – 15Combined sources6
Helixi16 – 28Combined sources13
Turni29 – 31Combined sources3
Beta strandi34 – 40Combined sources7
Beta strandi48 – 50Combined sources3
Beta strandi58 – 60Combined sources3
Beta strandi87 – 89Combined sources3
Helixi90 – 102Combined sources13
Turni103 – 108Combined sources6
Helixi113 – 129Combined sources17
Beta strandi141 – 147Combined sources7
Helixi154 – 156Combined sources3
Helixi157 – 169Combined sources13
Helixi172 – 174Combined sources3
Beta strandi175 – 182Combined sources8
Turni187 – 189Combined sources3
Helixi195 – 206Combined sources12
Beta strandi212 – 220Combined sources9
Helixi224 – 233Combined sources10
Helixi238 – 240Combined sources3
Beta strandi241 – 245Combined sources5
Helixi252 – 259Combined sources8
Helixi262 – 270Combined sources9
Beta strandi297 – 306Combined sources10
Helixi312 – 314Combined sources3
Helixi315 – 327Combined sources13
Beta strandi330 – 338Combined sources9
Helixi339 – 342Combined sources4
Helixi344 – 349Combined sources6
Helixi351 – 363Combined sources13
Beta strandi365 – 369Combined sources5
Helixi378 – 390Combined sources13
Beta strandi395 – 398Combined sources4
Helixi400 – 413Combined sources14
Beta strandi419 – 422Combined sources4
Beta strandi429 – 435Combined sources7
Beta strandi449 – 458Combined sources10
Helixi463 – 467Combined sources5
Beta strandi472 – 480Combined sources9
Beta strandi482 – 484Combined sources3
Helixi486 – 488Combined sources3
Helixi490 – 492Combined sources3
Beta strandi495 – 503Combined sources9
Beta strandi508 – 518Combined sources11
Beta strandi520 – 526Combined sources7
Helixi527 – 530Combined sources4
Helixi538 – 548Combined sources11
Helixi551 – 556Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V4UX-ray2.30A297-562[»]
2VKTX-ray2.50A297-562[»]
3IHLX-ray2.80A/B1-275[»]
ProteinModelPortaliQ9NRF8.
SMRiQ9NRF8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NRF8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 554Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST255

Sequence similaritiesi

Belongs to the CTP synthase family.Curated
Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG2387. Eukaryota.
COG0504. LUCA.
GeneTreeiENSGT00390000012473.
HOGENOMiHOG000077514.
HOVERGENiHBG002243.
InParanoidiQ9NRF8.
KOiK01937.
OMAiFEVNPNL.
OrthoDBiEOG091G03OK.
PhylomeDBiQ9NRF8.
TreeFamiTF300379.

Family and domain databases

CDDicd01746. GATase1_CTP_Synthase. 1 hit.
Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR033828. GATase1_CTP_Synthase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NRF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYILVTGGV ISGIGKGIIA SSIGTILKSC GLRVTAIKID PYINIDAGTF
60 70 80 90 100
SPYEHGEVFV LNDGGEVDLD LGNYERFLDI NLYKDNNITT GKIYQHVINK
110 120 130 140 150
ERRGDYLGKT VQVVPHITDA VQEWVMNQAK VPVDGNKEEP QICVIELGGT
160 170 180 190 200
IGDIEGMPFV EAFRQFQFKA KRENFCNIHV SLVPQLSATG EQKTKPTQNS
210 220 230 240 250
VRALRGLGLS PDLIVCRSST PIEMAVKEKI SMFCHVNPEQ VICIHDVSST
260 270 280 290 300
YRVPVLLEEQ SIVKYFKERL HLPIGDSASN LLFKWRNMAD RYERLQKICS
310 320 330 340 350
IALVGKYTKL RDCYASVFKA LEHSALAINH KLNLMYIDSI DLEKITETED
360 370 380 390 400
PVKFHEAWQK LCKADGILVP GGFGIRGTLG KLQAISWART KKIPFLGVCL
410 420 430 440 450
GMQLAVIEFA RNCLNLKDAD STEFRPNAPV PLVIDMPEHN PGNLGGTMRL
460 470 480 490 500
GIRRTVFKTE NSILRKLYGD VPFIEERHRH RFEVNPNLIK QFEQNDLSFV
510 520 530 540 550
GQDVDGDRME IIELANHPYF VGVQFHPEFS SRPMKPSPPY LGLLLAATGN
560 570 580
LNAYLQQGCK LSSSDRYSDA SDDSFSEPRI AELEIS
Length:586
Mass (Da):65,678
Last modified:October 1, 2000 - v1
Checksum:iAC1CF2E67D89741B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti220T → S in BAB14814 (PubMed:14702039).Curated1
Sequence conflicti233F → L in BAB14607 (PubMed:14702039).Curated1
Sequence conflicti304V → A in BAB14607 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226667 mRNA. Translation: AAF91241.1.
AK023549 mRNA. Translation: BAB14607.1.
AK024070 mRNA. Translation: BAB14814.1.
AK125332 mRNA. Translation: BAG54184.1.
AK125348 mRNA. Translation: BAG54188.1.
AL445467, AC073909 Genomic DNA. Translation: CAI40086.1.
CH471074 Genomic DNA. Translation: EAW98912.1.
BC006256 mRNA. Translation: AAH06256.2.
BC034986 mRNA. Translation: AAH34986.1.
CCDSiCCDS14175.1.
RefSeqiNP_001137474.1. NM_001144002.1.
NP_062831.3. NM_019857.4.
NP_787055.1. NM_175859.2.
XP_005274619.1. XM_005274562.3.
XP_006724566.1. XM_006724503.3.
UniGeneiHs.227049.

Genome annotation databases

EnsembliENST00000359276; ENSP00000352222; ENSG00000047230.
ENST00000380241; ENSP00000369590; ENSG00000047230.
ENST00000443824; ENSP00000401264; ENSG00000047230.
GeneIDi56474.
KEGGihsa:56474.
UCSCiuc004cxk.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226667 mRNA. Translation: AAF91241.1.
AK023549 mRNA. Translation: BAB14607.1.
AK024070 mRNA. Translation: BAB14814.1.
AK125332 mRNA. Translation: BAG54184.1.
AK125348 mRNA. Translation: BAG54188.1.
AL445467, AC073909 Genomic DNA. Translation: CAI40086.1.
CH471074 Genomic DNA. Translation: EAW98912.1.
BC006256 mRNA. Translation: AAH06256.2.
BC034986 mRNA. Translation: AAH34986.1.
CCDSiCCDS14175.1.
RefSeqiNP_001137474.1. NM_001144002.1.
NP_062831.3. NM_019857.4.
NP_787055.1. NM_175859.2.
XP_005274619.1. XM_005274562.3.
XP_006724566.1. XM_006724503.3.
UniGeneiHs.227049.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V4UX-ray2.30A297-562[»]
2VKTX-ray2.50A297-562[»]
3IHLX-ray2.80A/B1-275[»]
ProteinModelPortaliQ9NRF8.
SMRiQ9NRF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121144. 31 interactors.
IntActiQ9NRF8. 11 interactors.
MINTiMINT-1443035.
STRINGi9606.ENSP00000352222.

Protein family/group databases

MEROPSiC26.964.

PTM databases

iPTMnetiQ9NRF8.
PhosphoSitePlusiQ9NRF8.

Polymorphism and mutation databases

BioMutaiCTPS2.
DMDMi74752919.

Proteomic databases

EPDiQ9NRF8.
MaxQBiQ9NRF8.
PaxDbiQ9NRF8.
PeptideAtlasiQ9NRF8.
PRIDEiQ9NRF8.

Protocols and materials databases

DNASUi56474.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359276; ENSP00000352222; ENSG00000047230.
ENST00000380241; ENSP00000369590; ENSG00000047230.
ENST00000443824; ENSP00000401264; ENSG00000047230.
GeneIDi56474.
KEGGihsa:56474.
UCSCiuc004cxk.4. human.

Organism-specific databases

CTDi56474.
GeneCardsiCTPS2.
HGNCiHGNC:2520. CTPS2.
HPAiHPA017437.
MIMi300380. gene.
neXtProtiNX_Q9NRF8.
OpenTargetsiENSG00000047230.
PharmGKBiPA27021.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2387. Eukaryota.
COG0504. LUCA.
GeneTreeiENSGT00390000012473.
HOGENOMiHOG000077514.
HOVERGENiHBG002243.
InParanoidiQ9NRF8.
KOiK01937.
OMAiFEVNPNL.
OrthoDBiEOG091G03OK.
PhylomeDBiQ9NRF8.
TreeFamiTF300379.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciMetaCyc:HS00585-MONOMER.
ZFISH:HS00585-MONOMER.
BRENDAi6.3.4.2. 2681.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SIGNORiQ9NRF8.

Miscellaneous databases

ChiTaRSiCTPS2. human.
EvolutionaryTraceiQ9NRF8.
GeneWikiiCTPS2.
GenomeRNAii56474.
PROiQ9NRF8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000047230.
CleanExiHS_CTPS2.
ExpressionAtlasiQ9NRF8. baseline and differential.
GenevisibleiQ9NRF8. HS.

Family and domain databases

CDDicd01746. GATase1_CTP_Synthase. 1 hit.
Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR033828. GATase1_CTP_Synthase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRG2_HUMAN
AccessioniPrimary (citable) accession number: Q9NRF8
Secondary accession number(s): B3KWM2
, Q9BRI0, Q9H809, Q9H8K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.