Reviewed, UniProtKB/Swiss-Prot Q9NRD5 (PICK1_HUMAN)

Names and origin

Protein names

Recommended name:
    PRKCA-binding protein
Alternative name(s):
    Protein kinase C-alpha-binding protein
    Protein interacting with C kinase 1

Gene names

Name:	PICK1
Synonyms:	PRKCABP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. May be regulated upon PRKCA activation. May regulate ACCN3-ACCN2 heteromeric cation channel.
Subunit structure	Monomer and homodimer. Interacts presynaptically with the monoamine transporters SLC6A2 and SLC6A3; with the channel proteins ACCN1 and ACCN2; with the GTP-binding proteins ARF1 and ARF3. Interacts with PRKCA; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; and with ERBB2. Interacts with BTG2; with the glutamate receptors GRIA2, GRIA3, the isoform A of GRM4, GRM7 and GRM8; and with NAPA and NAPB. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with UNC5A By similarity. Interacts with CXADR. Interacts through its PDZ domain with the C-terminal tail of PRLHR.
Subcellular location	Cytoplasm › perinuclear region. Cell junction › synapse. Note: Also present at excitatory synapses.
Tissue specificity	Ubiquitous.
Post-translational modification	Phosphorylated By similarity.
Sequence similarities	Contains 1 AH domain. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm Synapse
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA methylation during embryonic development Non-traceable author statement. Source: UniProtKB DNA methylation during gametogenesis Traceable author statement. Source: UniProtKB activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Ref.1 Inferred from direct assay. Source: UniProtKB monoamine transport Ref.8 Inferred from direct assay. Source: UniProtKB neuronal ion channel clustering Ref.8 Traceable author statement. Source: UniProtKB protein amino acid phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB receptor clustering Inferred from sequence or structural similarity. Source: UniProtKB retrograde vesicle-mediated transport, Golgi to ER Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB presynaptic membrane Ref.8 Inferred from direct assay. Source: UniProtKB
Molecular function	ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB protein C-terminus binding Ref.8 Inferred from physical interaction. Source: UniProtKB protein kinase C binding Inferred from sequence or structural similarity. Source: UniProtKB receptor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ACCN1	Q16515	1	EBI-79165,EBI-79149
ACCN2	P78348	1	EBI-79165,EBI-79189

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 415

415

PRKCA-binding protein

PRO_0000058427

Regions

Domain

22 – 105

84

PDZ

Domain

144 – 357

214

AH

Compositional bias

382 – 388

7

Poly-Glu

Experimental info

Mutagenesis

27 – 28

2

KD → AA: Abolishes interaction with other proteins, but not with itself. Ref.1 Ref.8 Ref.9

Sequence conflict

7

1

Y → F in AAF97502. Ref.2

Sequence conflict

16

1

Missing in AAF97502. Ref.2

Sequence conflict

236

1

N → NI in AAF97502. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

415

Helix Strand Turn

Details...

Beta strand

21 – 26

6

Beta strand

34 – 39

6

Beta strand

47 – 52

6

Helix

57 – 61

5

Beta strand

69 – 73

5

Helix

83 – 92

10

Beta strand

95 – 102

8

Sequences

	Sequence		Length	Mass (Da)
	Q9NRD5-1 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: C569FD8AA5028B90	FASTA	415	46,600
	10 20 30 40 50 60 MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV QVFDNTPAAL 70 80 90 100 110 120 DGTVAAGDEI TGVNGRSIKG KTKVEVAKMI QEVKGEVTIH YNKLQADPKQ GMSLDIVLKK 130 140 150 160 170 180 VKHRLVENMS SGTADALGLS RAILCNDGLV KRLEELERTA ELYKGMTEHT KNLLRAFYEL 190 200 210 220 230 240 SQTHRAFGDV FSVIGVREPQ PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN 250 260 270 280 290 300 KAIPDTRLTI KKYLDVKFEY LSYCLKVKEM DDEEYSCIAL GEPLYRVSTG NYEYRLILRC 310 320 330 340 350 360 RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRLVST MSKYYNDCYA VLRDADVFPI 370 380 390 400 410 EVDLAHTTLA YGLNQEEFTD GEEEEEEEDT AAGEPSRDTR GAAGPLDKGG SWCDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors." Takeya R., Takeshige K., Sumimoto H. Biochem. Biophys. Res. Commun. 267:149-155(2000) [PubMed: 10623590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ARF1 AND ARF3, MUTAGENESIS OF 27-LYS-ASP-28. Tissue: B-cell.
[2]	"Protein kinases interacting with the human PICK1 protein." Zhu X., Chung I., Scholl P.R. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Reevaluating human gene annotation: a second-generation analysis of chromosome 22." Collins J.E., Goward M.E., Cole C.G., Smink L.J., Huckle E.J., Knowles S., Bye J.M., Beare D.M., Dunham I. Genome Res. 13:27-36(2003) [PubMed: 12529303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[7]	"The carboxyl terminus of the prolactin-releasing peptide receptor interacts with PDZ domain proteins involved in alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor clustering." Lin S.H.S., Arai A.C., Wang Z., Nothacker H.-P., Civelli O. Mol. Pharmacol. 60:916-923(2001) [PubMed: 11641419] [Abstract] Cited for: INTERACTION WITH PRLHR.
[8]	"Functional interaction between monoamine plasma membrane transporters and the synaptic PDZ domain-containing protein PICK1." Torres G.E., Yao W.-D., Mohn A.R., Quan H., Kim K.-M., Levey A.I., Staudinger J., Caron M.G. Neuron 30:121-134(2001) [PubMed: 11343649] [Abstract] Cited for: INTERACTION WITH SLC6A2 AND SLC6A3, MUTAGENESIS OF 27-LYS-ASP-28.
[9]	"Interaction of the synaptic protein PICK1 (protein interacting with C kinase 1) with the non-voltage gated sodium channels BNC1 (brain Na+ channel 1) and ASIC (acid-sensing ion channel)." Hruska-Hageman A.M., Wemmie J.A., Price M.P., Welsh M.J. Biochem. J. 361:443-450(2002) [PubMed: 11802773] [Abstract] Cited for: INTERACTION WITH ACCN1 AND ACCN2, MUTAGENESIS OF 27-LYS-ASP-28.
[10]	"A role for the PDZ-binding domain of the coxsackie B virus and adenovirus receptor (CAR) in cell adhesion and growth." Ashbourne-Excoffon K.J.D., Hruska-Hageman A.M., Klotz M., Traver G.L., Zabner J. J. Cell Sci. 117:4401-4409(2004) [PubMed: 15304526] [Abstract] Cited for: INTERACTION WITH CXADR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB026491 mRNA. Translation: BAA89294.1.
AF231710 mRNA. Translation: AAF97502.1.
AL049654 mRNA. Translation: CAB41082.1.
CR456550 mRNA. Translation: CAG30436.1.
AL031587 Genomic DNA. Translation: CAB37478.1.
BC017561 mRNA. Translation: AAH17561.1.

IPI

IPI00160369.

PIR

JC7167.

RefSeq

NP_001034672.1.
NP_001034673.1.
NP_036539.1.

UniGene

Hs.180871

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GZV	X-ray	1.12	A	19-105	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NRD5. 15 interactions.

Proteomic databases

PRIDE

Q9NRD5.

Genome annotation databases

Ensembl

ENSG00000100151. Homo sapiens. [Contig view]

GeneID

9463.

KEGG

hsa:9463.

Organism-specific databases

GeneCards

GC22P036783.

H-InvDB

HIX0016462.

HGNC

HGNC:9394. PICK1.

MIM

605926. gene.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9NRD5.

HOVERGEN

Q9NRD5.

OMA

Q9NRD5. YVVQVFD.

Gene expression databases

ArrayExpress

Q9NRD5.

Bgee

Q9NRD5.

CleanEx

HS_PICK1.

GermOnline

ENSG00000100151. Homo sapiens.

Family and domain databases

InterPro

IPR010504. Arfaptin.
IPR001478. PDZ/DHR/GLGF.
[Graphical view]

Gene3D

G3DSA:1.20.1270.60. Arfaptin. 1 hit.

Pfam

PF06456. Arfaptin. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]

SMART

SM00228. PDZ. 1 hit.
[Graphical view]

PROSITE

PS50870. AH. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

35452.

SOURCE

Search...

Entry information

Entry name

PICK1_HUMAN

Accession

Primary (citable) accession number: Q9NRD5
Secondary accession number(s): O95906

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	August 2, 2002
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)