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Protein

Carbohydrate sulfotransferase 12

Gene

CHST12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 177PAPSBy similarity7
Nucleotide bindingi245 – 253PAPSBy similarity9

GO - Molecular functioni

  • 3'-phosphoadenosine 5'-phosphosulfate binding Source: UniProtKB
  • chondroitin 4-sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • carbohydrate biosynthetic process Source: InterPro
  • chondroitin sulfate biosynthetic process Source: UniProtKB
  • dermatan sulfate biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS06130-MONOMER.
BRENDAi2.8.2.5. 2681.
ReactomeiR-HSA-2022870. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 12 (EC:2.8.2.5)
Alternative name(s):
Chondroitin 4-O-sulfotransferase 2
Chondroitin 4-sulfotransferase 2
Short name:
C4ST-2
Short name:
C4ST2
Sulfotransferase Hlo
Gene namesi
Name:CHST12
ORF Names:UNQ500/PRO1017
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000136213.9.
HGNCiHGNC:17423. CHST12.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini27 – 414LumenalSequence analysisAdd BLAST388

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi55501.
OpenTargetsiENSG00000136213.
PharmGKBiPA134969008.

Polymorphism and mutation databases

BioMutaiCHST12.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896681 – 414Carbohydrate sulfotransferase 12Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi370N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9NRB3.
MaxQBiQ9NRB3.
PaxDbiQ9NRB3.
PeptideAtlasiQ9NRB3.
PRIDEiQ9NRB3.

PTM databases

iPTMnetiQ9NRB3.
PhosphoSitePlusiQ9NRB3.

Expressioni

Tissue specificityi

Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung.1 Publication

Gene expression databases

BgeeiENSG00000136213.
CleanExiHS_CHST12.
ExpressionAtlasiQ9NRB3. baseline and differential.
GenevisibleiQ9NRB3. HS.

Organism-specific databases

HPAiHPA041680.

Interactioni

Protein-protein interaction databases

BioGridi120681. 28 interactors.
IntActiQ9NRB3. 4 interactors.
STRINGi9606.ENSP00000258711.

Structurei

3D structure databases

ProteinModelPortaliQ9NRB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG097841.
InParanoidiQ9NRB3.
KOiK04742.
OMAiYGKFSRH.
OrthoDBiEOG091G0FZO.
PhylomeDBiQ9NRB3.
TreeFamiTF325581.

Family and domain databases

InterProiView protein in InterPro
IPR018011. Carb_sulfotrans_8-10.
IPR005331. Sulfotransferase.
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiView protein in Pfam
PF03567. Sulfotransfer_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NRB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKARLFRLW LVLGSVFMIL LIIVYWDSAG AAHFYLHTSF SRPHTGPPLP
60 70 80 90 100
TPGPDRDREL TADSDVDEFL DKFLSAGVKQ SDLPRKETEQ PPAPGSMEES
110 120 130 140 150
VRGYDWSPRD ARRSPDQGRQ QAERRSVLRG FCANSSLAFP TKERAFDDIP
160 170 180 190 200
NSELSHLIVD DRHGAIYCYV PKVACTNWKR VMIVLSGSLL HRGAPYRDPL
210 220 230 240 250
RIPREHVHNA SAHLTFNKFW RRYGKLSRHL MKVKLKKYTK FLFVRDPFVR
260 270 280 290 300
LISAFRSKFE LENEEFYRKF AVPMLRLYAN HTSLPASARE AFRAGLKVSF
310 320 330 340 350
ANFIQYLLDP HTEKLAPFNE HWRQVYRLCH PCQIDYDFVG KLETLDEDAA
360 370 380 390 400
QLLQLLQVDR QLRFPPSYRN RTASSWEEDW FAKIPLAWRQ QLYKLYEADF
410
VLFGYPKPEN LLRD
Length:414
Mass (Da):48,414
Last modified:March 15, 2005 - v2
Checksum:i8730D8731F623078
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30G → S in AAF81692 (PubMed:10781601).Curated1
Sequence conflicti42R → K in AAF81692 (PubMed:10781601).Curated1
Sequence conflicti92P → L in AAF81692 (PubMed:10781601).Curated1
Sequence conflicti380W → C in AAF81692 (PubMed:10781601).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02147152P → H. Corresponds to variant dbSNP:rs3735099Ensembl.1
Natural variantiVAR_02147261T → S. Corresponds to variant dbSNP:rs3735100Ensembl.1
Natural variantiVAR_03373894P → L. Corresponds to variant dbSNP:rs12536223Ensembl.1
Natural variantiVAR_021473109R → S. Corresponds to variant dbSNP:rs17132395Ensembl.1
Natural variantiVAR_021474145A → P. Corresponds to variant dbSNP:rs17132399Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239822 mRNA. Translation: AAF81692.1.
AJ289131 mRNA. Translation: CAB92133.1.
AY358574 mRNA. Translation: AAQ88937.1.
AK313484 mRNA. Translation: BAG36268.1.
CH236953 Genomic DNA. Translation: EAL23955.1.
BC002918 mRNA. Translation: AAH02918.1.
BC095492 mRNA. Translation: AAH95492.1.
CCDSiCCDS5333.1.
RefSeqiNP_001230723.1. NM_001243794.1.
NP_001230724.1. NM_001243795.1.
NP_061111.1. NM_018641.4.
XP_011513745.1. XM_011515443.2.
XP_011513746.1. XM_011515444.2.
UniGeneiHs.288853.
Hs.744987.

Genome annotation databases

EnsembliENST00000258711; ENSP00000258711; ENSG00000136213.
ENST00000618655; ENSP00000481912; ENSG00000136213.
GeneIDi55501.
KEGGihsa:55501.
UCSCiuc003smc.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHSTC_HUMAN
AccessioniPrimary (citable) accession number: Q9NRB3
Secondary accession number(s): A4D1Z9, Q502W3, Q9NXY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 27, 2017
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families