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Protein

Carbohydrate sulfotransferase 12

Gene

CHST12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 177PAPSBy similarity7
Nucleotide bindingi245 – 253PAPSBy similarity9

GO - Molecular functioni

  • 3'-phosphoadenosine 5'-phosphosulfate binding Source: UniProtKB
  • chondroitin 4-sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • carbohydrate biosynthetic process Source: InterPro
  • chondroitin sulfate biosynthetic process Source: UniProtKB
  • dermatan sulfate biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS06130-MONOMER
BRENDAi2.8.2.5 2681
ReactomeiR-HSA-2022870 Chondroitin sulfate biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 12 (EC:2.8.2.5)
Alternative name(s):
Chondroitin 4-O-sulfotransferase 2
Chondroitin 4-sulfotransferase 2
Short name:
C4ST-2
Short name:
C4ST2
Sulfotransferase Hlo
Gene namesi
Name:CHST12
ORF Names:UNQ500/PRO1017
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000136213.9
HGNCiHGNC:17423 CHST12
MIMi610129 gene
neXtProtiNX_Q9NRB3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini27 – 414LumenalSequence analysisAdd BLAST388

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi55501
OpenTargetsiENSG00000136213
PharmGKBiPA134969008

Polymorphism and mutation databases

BioMutaiCHST12

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896681 – 414Carbohydrate sulfotransferase 12Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi370N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9NRB3
MaxQBiQ9NRB3
PaxDbiQ9NRB3
PeptideAtlasiQ9NRB3
PRIDEiQ9NRB3

PTM databases

iPTMnetiQ9NRB3
PhosphoSitePlusiQ9NRB3

Expressioni

Tissue specificityi

Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung.1 Publication

Gene expression databases

BgeeiENSG00000136213
CleanExiHS_CHST12
ExpressionAtlasiQ9NRB3 baseline and differential
GenevisibleiQ9NRB3 HS

Organism-specific databases

HPAiHPA041680

Interactioni

Protein-protein interaction databases

BioGridi120681, 28 interactors
IntActiQ9NRB3, 4 interactors
STRINGi9606.ENSP00000258711

Structurei

3D structure databases

ProteinModelPortaliQ9NRB3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651 Eukaryota
ENOG4111GJR LUCA
GeneTreeiENSGT00760000119214
HOGENOMiHOG000231801
HOVERGENiHBG097841
InParanoidiQ9NRB3
KOiK04742
OMAiDIPNYEL
OrthoDBiEOG091G0FZO
PhylomeDBiQ9NRB3
TreeFamiTF325581

Family and domain databases

InterProiView protein in InterPro
IPR018011 Carb_sulfotrans_8-10
IPR005331 Sulfotransferase
PANTHERiPTHR12137 PTHR12137, 1 hit
PfamiView protein in Pfam
PF03567 Sulfotransfer_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NRB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKARLFRLW LVLGSVFMIL LIIVYWDSAG AAHFYLHTSF SRPHTGPPLP
60 70 80 90 100
TPGPDRDREL TADSDVDEFL DKFLSAGVKQ SDLPRKETEQ PPAPGSMEES
110 120 130 140 150
VRGYDWSPRD ARRSPDQGRQ QAERRSVLRG FCANSSLAFP TKERAFDDIP
160 170 180 190 200
NSELSHLIVD DRHGAIYCYV PKVACTNWKR VMIVLSGSLL HRGAPYRDPL
210 220 230 240 250
RIPREHVHNA SAHLTFNKFW RRYGKLSRHL MKVKLKKYTK FLFVRDPFVR
260 270 280 290 300
LISAFRSKFE LENEEFYRKF AVPMLRLYAN HTSLPASARE AFRAGLKVSF
310 320 330 340 350
ANFIQYLLDP HTEKLAPFNE HWRQVYRLCH PCQIDYDFVG KLETLDEDAA
360 370 380 390 400
QLLQLLQVDR QLRFPPSYRN RTASSWEEDW FAKIPLAWRQ QLYKLYEADF
410
VLFGYPKPEN LLRD
Length:414
Mass (Da):48,414
Last modified:March 15, 2005 - v2
Checksum:i8730D8731F623078
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30G → S in AAF81692 (PubMed:10781601).Curated1
Sequence conflicti42R → K in AAF81692 (PubMed:10781601).Curated1
Sequence conflicti92P → L in AAF81692 (PubMed:10781601).Curated1
Sequence conflicti380W → C in AAF81692 (PubMed:10781601).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02147152P → H. Corresponds to variant dbSNP:rs3735099Ensembl.1
Natural variantiVAR_02147261T → S. Corresponds to variant dbSNP:rs3735100Ensembl.1
Natural variantiVAR_03373894P → L. Corresponds to variant dbSNP:rs12536223Ensembl.1
Natural variantiVAR_021473109R → S. Corresponds to variant dbSNP:rs17132395Ensembl.1
Natural variantiVAR_021474145A → P. Corresponds to variant dbSNP:rs17132399Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239822 mRNA Translation: AAF81692.1
AJ289131 mRNA Translation: CAB92133.1
AY358574 mRNA Translation: AAQ88937.1
AK313484 mRNA Translation: BAG36268.1
CH236953 Genomic DNA Translation: EAL23955.1
BC002918 mRNA Translation: AAH02918.1
BC095492 mRNA Translation: AAH95492.1
CCDSiCCDS5333.1
RefSeqiNP_001230723.1, NM_001243794.1
NP_001230724.1, NM_001243795.1
NP_061111.1, NM_018641.4
XP_011513745.1, XM_011515443.2
XP_011513746.1, XM_011515444.2
UniGeneiHs.288853
Hs.744987

Genome annotation databases

EnsembliENST00000258711; ENSP00000258711; ENSG00000136213
ENST00000618655; ENSP00000481912; ENSG00000136213
GeneIDi55501
KEGGihsa:55501
UCSCiuc003smc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHSTC_HUMAN
AccessioniPrimary (citable) accession number: Q9NRB3
Secondary accession number(s): A4D1Z9, Q502W3, Q9NXY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health