Q9NR81 (ARHG3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho guanine nucleotide exchange factor 3

Alternative name(s):
Exchange factor found in platelets and leukemic and neuronal tissues
Short name=XPLN

Gene names

Name:

ARHGEF3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases. Ref.8
Subunit structure	Interacts with RHOA and RHOB.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney. Ref.1 Ref.8
Sequence similarities	Contains 1 DH (DBL-homology) domain. Contains 1 PH domain.
Sequence caution	The sequence AAH68513.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14891.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD92898.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Traceable author statement Ref.1. Source: ProtInc apoptotic process Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	Rho guanyl-nucleotide exchange factor activity Traceable author statement Ref.1. Source: ProtInc phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NR81-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NR81-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MVAKDYPFYLTVKRANCSLELPPASGPAKDAE → MDSSTAMNQC...AVSLCSLDIS

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NR81-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MVAKDYPFYLTVKRANCSLELPPASGPAKDAE → MIEVCHHNWLLWLCPSKFGIFMCCLASTTGQMELRRTR

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NR81-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-202: Missing.
     203-204: GW → MK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Rho guanine nucleotide exchange factor 3		PRO_0000080911
Regions
	Domain	122 – 304	183	DH
	Domain	291 – 449	159	PH
Natural variations
	Alternative sequence	1 – 202	202	Missing in isoform 4.		VSP_027204
	Alternative sequence	1 – 32	32	MVAKD…AKDAE → MDSSTAMNQCSCRGMEENKE RPKRQRQNNFPMFPSPKAWN FRGRKRKQSTQDEDAVSLCS LDIS in isoform 2.		VSP_011612
	Alternative sequence	1 – 32	32	MVAKD…AKDAE → MIEVCHHNWLLWLCPSKFGI FMCCLASTTGQMELRRTR in isoform 3.		VSP_027205
	Alternative sequence	203 – 204	2	GW → MK in isoform 4.		VSP_027206
	Natural variant	13	1	K → R. Corresponds to variant rs3732507 [ dbSNP | Ensembl ].		VAR_021935
	Natural variant	335	1	L → V. Ref.3 Ref.4 Ref.6 Ref.7 Corresponds to variant rs3772219 [ dbSNP | Ensembl ].		VAR_021936
Experimental info
	Sequence conflict	410	1	I → T in AAP97313. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0FE8248AB52D1CBC	FASTA	526	59,783
	10 20 30 40 50 60 MVAKDYPFYL TVKRANCSLE LPPASGPAKD AEEPSNKRVK PLSRVTSLAN LIPPVKATPL 70 80 90 100 110 120 KRFSQTLQRS ISFRSESRPD ILAPRPWSRN AAPSSTKRRD SKLWSETFDV CVNQMLTSKE 130 140 150 160 170 180 IKRQEAIFEL SQGEEDLIED LKLAKKAYHD PMLKLSIMTE QELNQIFGTL DSLIPLHEEL 190 200 210 220 230 240 LSQLRDVRKP DGSTEHVGPI LVGWLPCLSS YDSYCSNQVA AKALLDHKKQ DHRVQDFLQR 250 260 270 280 290 300 CLESPFSRKL DLWNFLDIPR SRLVKYPLLL REILRHTPND NPDQQHLEEA INIIQGIVAE 310 320 330 340 350 360 INTKTGESEC RYYKERLLYL EEGQKDSLID SSRVLCCHGE LKNNRGVKLH VFLFQEVLVI 370 380 390 400 410 420 TRAVTHNEQL CYQLYRQPIP VKDLLLEDLQ DGEVRLGGSL RGAFSNNERI KNFFRVSFKN 430 440 450 460 470 480 GSQSQTHSLQ ANDTFNKQQW LNCIRQAKET VLCAAGQAGV LDSEGSFLNP TTGSRELQGE 490 500 510 520 TKLEQMDQSD SESDCSMDTS EVSLDCERME QTDSSCGNSR HGESNV
	Isoform 2 [UniParc]. Checksum: C01C434F0858A58C	FASTA	558	63,755
	10 20 30 40 50 60 MDSSTAMNQC SCRGMEENKE RPKRQRQNNF PMFPSPKAWN FRGRKRKQST QDEDAVSLCS 70 80 90 100 110 120 LDISEPSNKR VKPLSRVTSL ANLIPPVKAT PLKRFSQTLQ RSISFRSESR PDILAPRPWS 130 140 150 160 170 180 RNAAPSSTKR RDSKLWSETF DVCVNQMLTS KEIKRQEAIF ELSQGEEDLI EDLKLAKKAY 190 200 210 220 230 240 HDPMLKLSIM TEQELNQIFG TLDSLIPLHE ELLSQLRDVR KPDGSTEHVG PILVGWLPCL 250 260 270 280 290 300 SSYDSYCSNQ VAAKALLDHK KQDHRVQDFL QRCLESPFSR KLDLWNFLDI PRSRLVKYPL 310 320 330 340 350 360 LLREILRHTP NDNPDQQHLE EAINIIQGIV AEINTKTGES ECRYYKERLL YLEEGQKDSL 370 380 390 400 410 420 IDSSRVLCCH GELKNNRGVK LHVFLFQEVL VITRAVTHNE QLCYQLYRQP IPVKDLLLED 430 440 450 460 470 480 LQDGEVRLGG SLRGAFSNNE RIKNFFRVSF KNGSQSQTHS LQANDTFNKQ QWLNCIRQAK 490 500 510 520 530 540 ETVLCAAGQA GVLDSEGSFL NPTTGSRELQ GETKLEQMDQ SDSESDCSMD TSEVSLDCER 550 MEQTDSSCGN SRHGESNV
	Isoform 3 [UniParc]. Checksum: 64940517719915C0	FASTA	532	60,813
	10 20 30 40 50 60 MIEVCHHNWL LWLCPSKFGI FMCCLASTTG QMELRRTREP SNKRVKPLSR VTSLANLIPP 70 80 90 100 110 120 VKATPLKRFS QTLQRSISFR SESRPDILAP RPWSRNAAPS STKRRDSKLW SETFDVCVNQ 130 140 150 160 170 180 MLTSKEIKRQ EAIFELSQGE EDLIEDLKLA KKAYHDPMLK LSIMTEQELN QIFGTLDSLI 190 200 210 220 230 240 PLHEELLSQL RDVRKPDGST EHVGPILVGW LPCLSSYDSY CSNQVAAKAL LDHKKQDHRV 250 260 270 280 290 300 QDFLQRCLES PFSRKLDLWN FLDIPRSRLV KYPLLLREIL RHTPNDNPDQ QHLEEAINII 310 320 330 340 350 360 QGIVAEINTK TGESECRYYK ERLLYLEEGQ KDSLIDSSRV LCCHGELKNN RGVKLHVFLF 370 380 390 400 410 420 QEVLVITRAV THNEQLCYQL YRQPIPVKDL LLEDLQDGEV RLGGSLRGAF SNNERIKNFF 430 440 450 460 470 480 RVSFKNGSQS QTHSLQANDT FNKQQWLNCI RQAKETVLCA AGQAGVLDSE GSFLNPTTGS 490 500 510 520 530 RELQGETKLE QMDQSDSESD CSMDTSEVSL DCERMEQTDS SCGNSRHGES NV
	Isoform 4 [UniParc]. Checksum: DF90022C9392296E	FASTA	324	36,981
	10 20 30 40 50 60 MKLPCLSSYD SYCSNQVAAK ALLDHKKQDH RVQDFLQRCL ESPFSRKLDL WNFLDIPRSR 70 80 90 100 110 120 LVKYPLLLRE ILRHTPNDNP DQQHLEEAIN IIQGIVAEIN TKTGESECRY YKERLLYLEE 130 140 150 160 170 180 GQKDSLIDSS RVLCCHGELK NNRGVKLHVF LFQEVLVITR AVTHNEQLCY QLYRQPIPVK 190 200 210 220 230 240 DLLLEDLQDG EVRLGGSLRG AFSNNERIKN FFRVSFKNGS QSQTHSLQAN DTFNKQQWLN 250 260 270 280 290 300 CIRQAKETVL CAAGQAGVLD SEGSFLNPTT GSRELQGETK LEQMDQSDSE SDCSMDTSEV 310 320 SLDCERMEQT DSSCGNSRHG ESNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22." Thiesen S., Kuebart S., Ropers H.-H., Nothwang H.G. Biochem. Biophys. Res. Commun. 273:364-369(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	Guo J.H., Yu L. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-335.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-526 (ISOFORMS 1/2/3), VARIANT VAL-335. Tissue: Brain and Placenta.
[5]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-335.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), VARIANT VAL-335. Tissue: Hippocampus and Uterus.
[8]	"XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC." Arthur W.T., Ellerbroek S.M., Der C.J., Burridge K., Wennerberg K. J. Biol. Chem. 277:42964-42972(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF249744 mRNA. Translation: AAF79954.1. AL136832 mRNA. Translation: CAB66766.1. AF433662 mRNA. Translation: AAP97313.1. AK024340 mRNA. Translation: BAB14891.1. Different initiation. AK291412 mRNA. Translation: BAF84101.1. AB209661 mRNA. Translation: BAD92898.1. Different initiation. CH471055 Genomic DNA. Translation: EAW65326.1. BC054345 mRNA. Translation: AAH54345.2. BC068513 mRNA. Translation: AAH68513.1. Different initiation. BC098122 mRNA. Translation: AAH98122.2. BC098272 mRNA. Translation: AAH98272.2. BC099715 mRNA. Translation: AAH99715.1. BC104723 mRNA. Translation: AAI04724.2.
IPI	IPI00020949. IPI00385169. IPI00854602. IPI00945383.
RefSeq	NP_001122087.1. NM_001128615.1. NP_001122088.1. NM_001128616.1. NP_062455.1. NM_019555.2.
UniGene	Hs.476402.
3D structure databases
ProteinModelPortal	Q9NR81.
ModBase	Search...
PTM databases
PhosphoSite	Q9NR81.
Polymorphism databases
DMDM	52782760.
Proteomic databases
PaxDb	Q9NR81.
PRIDE	Q9NR81.
Protocols and materials databases
DNASU	50650.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000296315; ENSP00000296315; ENSG00000163947. ENST00000338458; ENSP00000341071; ENSG00000163947. ENST00000413728; ENSP00000410922; ENSG00000163947.
GeneID	50650.
KEGG	hsa:50650.
UCSC	uc003dif.2. human. uc003dig.2. human. uc003dih.2. human. uc010hmy.1. human.
Organism-specific databases
CTD	50650.
GeneCards	GC03M056736.
HGNC	HGNC:683. ARHGEF3.
HPA	HPA034715.
MIM	612115. gene.
neXtProt	NX_Q9NR81.
PharmGKB	PA24973.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5422.
HOVERGEN	HBG050567.
OMA	TKLEQMD.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9NR81.
Bgee	Q9NR81.
CleanEx	HS_ARHGEF3.
Genevestigator	Q9NR81.
GermOnline	ENSG00000163947. Homo sapiens.
Family and domain databases
Gene3D	1.20.900.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR000219. DH-domain. IPR001331. GDS_CDC24_CS. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR015721. RhoGEF-like. [Graphical view]
PANTHER	PTHR22825. PTHR22825. 1 hit.
Pfam	PF00621. RhoGEF. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. [Graphical view]
SUPFAM	SSF48065. DH-domain. 1 hit.
PROSITE	PS00741. DH_1. 1 hit. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ARHGEF3. human.
GenomeRNAi	50650.
NextBio	53200.
SOURCE	Search...

Entry information

Entry name

ARHG3_HUMAN

Accession

Primary (citable) accession number: Q9NR81
Secondary accession number(s): A8K5U7 , Q4FZB6, Q4QQI5, Q4QQQ0, Q59F00, Q6NUN3, Q7Z4U2, Q7Z5T2, Q9H7T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.