Delta-like protein 4 precursor - Homo sapiens (Human)

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Q9NR61 (DLL4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Delta-like protein 4

Alternative name(s):
Drosophila Delta homolog 4
Short name=Delta4

Gene names

Name:	DLL4
ORF Names:	UNQ1895/PRO4341

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4 By similarity.
Subunit structure	Binds to Notch-1 and Notch-4 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Probable.
Tissue specificity	Expressed in vascular endothelium.
Domain	The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
Post-translational modification	Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation By similarity.
Sequence similarities	Contains 1 DSL domain. Contains 8 EGF-like domains.

Ontologies

Keywords
Biological process	Differentiation Notch signaling pathway
Cellular component	Membrane
Domain	EGF-like domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	Notch receptor processing Traceable author statement. Source: Reactome Notch signaling pathway Traceable author statement. Source: Reactome blood circulation Traceable author statement. Source: ProtInc cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular function	Notch binding Traceable author statement. Source: ProtInc calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Ref.6

Chain

27 – 685

659

Delta-like protein 4

PRO_0000007512

Regions

Topological domain

27 – 529

503

Extracellular Potential

Transmembrane

530 – 550

Helical; Potential

Topological domain

551 – 685

135

Cytoplasmic Potential

Domain

173 – 217

DSL

Domain

218 – 251

EGF-like 1

Domain

252 – 282

EGF-like 2

Domain

284 – 322

EGF-like 3

Domain

324 – 360

EGF-like 4

Domain

362 – 400

EGF-like 5

Domain

402 – 438

EGF-like 6

Domain

440 – 476

EGF-like 7

Domain

480 – 518

EGF-like 8

Amino acid modifications

Glycosylation

108

N-linked (GlcNAc...) Potential

Glycosylation

183

N-linked (GlcNAc...) Potential

Glycosylation

205

N-linked (GlcNAc...) Potential

Glycosylation

393

N-linked (GlcNAc...) Potential

Disulfide bond

175 ↔ 184

By similarity

Disulfide bond

188 ↔ 200

By similarity

Disulfide bond

208 ↔ 217

By similarity

Disulfide bond

222 ↔ 233

By similarity

Disulfide bond

226 ↔ 239

By similarity

Disulfide bond

241 ↔ 250

By similarity

Disulfide bond

253 ↔ 264

By similarity

Disulfide bond

259 ↔ 270

By similarity

Disulfide bond

272 ↔ 281

By similarity

Disulfide bond

288 ↔ 300

By similarity

Disulfide bond

294 ↔ 310

By similarity

Disulfide bond

312 ↔ 321

By similarity

Disulfide bond

328 ↔ 339

By similarity

Disulfide bond

333 ↔ 348

By similarity

Disulfide bond

350 ↔ 359

By similarity

Disulfide bond

366 ↔ 377

By similarity

Disulfide bond

371 ↔ 388

By similarity

Disulfide bond

390 ↔ 399

By similarity

Disulfide bond

406 ↔ 417

By similarity

Disulfide bond

411 ↔ 426

By similarity

Disulfide bond

428 ↔ 437

By similarity

Disulfide bond

444 ↔ 455

By similarity

Disulfide bond

449 ↔ 464

By similarity

Disulfide bond

466 ↔ 475

By similarity

Disulfide bond

484 ↔ 495

By similarity

Disulfide bond

489 ↔ 506

By similarity

Disulfide bond

508 ↔ 517

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9NR61 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 6CF89D3C220ACC89
Show »

FASTA

685

74,605

        10         20         30         40         50         60 
MAAASRSASG WALLLLVALW QQRAAGSGVF QLQLQEFINE RGVLASGRPC EPGCRTFFRV 

        70         80         90        100        110        120 
CLKHFQAVVS PGPCTFGTVS TPVLGTNSFA VRDDSSGGGR NPLQLPFNFT WPGTFSLIIE 

       130        140        150        160        170        180 
AWHAPGDDLR PEALPPDALI SKIAIQGSLA VGQNWLLDEQ TSTLTRLRYS YRVICSDNYY 

       190        200        210        220        230        240 
GDNCSRLCKK RNDHFGHYVC QPDGNLSCLP GWTGEYCQQP ICLSGCHEQN GYCSKPAECL 

       250        260        270        280        290        300 
CRPGWQGRLC NECIPHNGCR HGTCSTPWQC TCDEGWGGLF CDQDLNYCTH HSPCKNGATC 

       310        320        330        340        350        360 
SNSGQRSYTC TCRPGYTGVD CELELSECDS NPCRNGGSCK DQEDGYHCLC PPGYYGLHCE 

       370        380        390        400        410        420 
HSTLSCADSP CFNGGSCRER NQGANYACEC PPNFTGSNCE KKVDRCTSNP CANGGQCLNR 

       430        440        450        460        470        480 
GPSRMCRCRP GFTGTYCELH VSDCARNPCA HGGTCHDLEN GLMCTCPAGF SGRRCEVRTS 

       490        500        510        520        530        540 
IDACASSPCF NRATCYTDLS TDTFVCNCPY GFVGSRCEFP VGLPPSFPWV AVSLGVGLAV 

       550        560        570        580        590        600 
LLVLLGMVAV AVRQLRLRRP DDGSREAMNN LSDFQKDNLI PAAQLKNTNQ KKELEVDCGL 

       610        620        630        640        650        660 
DKSNCGKQQN HTLDYNLAPG PLGRGTMPGK FPHSDKSLGE KAPLRLHSEK PECRISAICS 

       670        680 
PRDSMYQSVC LISEERNECV IATEV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dll4, a novel Notch ligand expressed in arterial endothelium." Shutter J.R., Scully S., Fan W., Richards W.G., Kitajewski J., Deblandre G.A., Kintner C.R., Stark K.L. Genes Dev. 14:1313-1318(2000) [PubMed: 10837024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Human Delta-2, Notch ligand gene." Sakano S. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Molecular cloning of Delta-4, a new mouse and human Notch ligand." Yoneya T., Tahara T., Nagao K., Yamada Y., Yamamoto T., Miyatani S., Nishikawa M. J. Biochem. 129:27-34(2001) [PubMed: 11134954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[7]	"A novel Delta gene expressed in embryonic and tumour vasculature." Mailhos C., Modlich U., Lewis J., Harris A., Bicknell R., Ish-Horowicz D. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-685. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF253468 mRNA. Translation: AAF76427.1. AB036931 mRNA. Translation: BAB16085.1. AB043894 mRNA. Translation: BAB18581.1. AY358894 mRNA. Translation: AAQ89253.1. BC106950 mRNA. Translation: AAI06951.1. AF279305 mRNA. Translation: AAF81912.1.
IPI	IPI00015994.
PIR	JC7570.
RefSeq	NP_061947.1. NM_019074.3.
UniGene	Hs.511076.
3D structure databases
ProteinModelPortal	Q9NR61.
SMR	Q9NR61. Positions 174-521.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NR61.
Polymorphism databases
DMDM	13431490.
Proteomic databases
PRIDE	Q9NR61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000249749; ENSP00000249749; ENSG00000128917.
GeneID	54567.
KEGG	hsa:54567.
UCSC	uc001zng.1. human.
Organism-specific databases
CTD	54567.
GeneCards	GC15P041221.
H-InvDB	HIX0202183.
HGNC	HGNC:2910. DLL4.
HPA	HPA023392.
MIM	605185. gene.
neXtProt	NX_Q9NR61.
PharmGKB	PA27366.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11597.
GeneTree	ENSGT00600000084206.
HOGENOM	HBG403082.
HOVERGEN	HBG007139.
InParanoid	Q9NR61.
OMA	IIEAWHA.
OrthoDB	EOG4PZJ63.
PhylomeDB	Q9NR61.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9NR61.
Bgee	Q9NR61.
CleanEx	HS_DLL4.
Genevestigator	Q9NR61.
GermOnline	ENSG00000128917. Homo sapiens.
Family and domain databases
InterPro	IPR001774. DSL. IPR006209. EGF. IPR006210. EGF-like. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR000742. EGF_3. IPR011651. Notch_ligand_N. [Graphical view]
KO	K06051.
Pfam	PF01414. DSL. 1 hit. PF00008. EGF. 4 hits. PF07657. MNNL. 1 hit. [Graphical view]
SMART	SM00051. DSL. 1 hit. SM00181. EGF. 6 hits. SM00179. EGF_CA. 2 hits. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS51051. DSL. 1 hit. PS00022. EGF_1. 8 hits. PS01186. EGF_2. 7 hits. PS50026. EGF_3. 8 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	57056.
SOURCE	Search...

Entry information

Entry name

DLL4_HUMAN

Accession

Primary (citable) accession number: Q9NR61
Secondary accession number(s): Q3KP23, Q9NQT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents