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Q9NR34 (MA1C1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IC

EC=3.2.1.113
Alternative name(s):
HMIC
Mannosidase alpha class 1C member 1
Processing alpha-1,2-mannosidase IC
Short name=Alpha-1,2-mannosidase IC
Gene names
Name:MAN1C1
Synonyms:MAN1A3, MAN1C
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce first Man8GlcNAc2 then Man6GlcNAc and a small amount of Man5GlcNAc.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Calcium.

Enzyme regulation

Inhibited by both 1-deoxymannojirimycin and kifunensine.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

Expressed in most tissues with the exception of lung, muscle and pancreas. Highly expressed in placenta.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 630630Mannosyl-oligosaccharide 1,2-alpha-mannosidase IC
PRO_0000210315

Regions

Topological domain1 – 2222Cytoplasmic Potential
Transmembrane23 – 4321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain44 – 630587Lumenal Potential

Amino acid modifications

Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation6181N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9NR34 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 80FFC71EFB36552A

FASTA63070,911
        10         20         30         40         50         60 
MLMRKVPGFV PASPWGLRLP QKFLFLLFLS GLVTLCFGAL FLLPHSSRLK RLFLAPRTQQ 

        70         80         90        100        110        120 
PGLEVVAEIA GHAPAREQEP PPNPAPAAPA PGEDDPSSWA SPRRRKGGLR RTRPTGPREE 

       130        140        150        160        170        180 
ATAARGNSIP ASRPGDEGVP FRFDFNAFRS RLRHPVLGTR ADESQEPQSQ VRAQREKIKE 

       190        200        210        220        230        240 
MMQFAWQSYK RYAMGKNELR PLTKDGYEGN MFGGLSGATV IDSLDTLYLM ELKEEFQEAK 

       250        260        270        280        290        300 
AWVGESFHLN VSGEASLFEV NIRYIGGLLS AFYLTGEEVF RIKAIRLGEK LLPAFNTPTG 

       310        320        330        340        350        360 
IPKGVVSFKS GNWGWATAGS SSILAEFGSL HLEFLHLTEL SGNQVFAEKV RNIRKVLRKI 

       370        380        390        400        410        420 
EKPFGLYPNF LSPVSGNWVQ HHVSVGGLGD SFYEYLIKSW LMSGKTDMEA KNMYYEALEA 

       430        440        450        460        470        480 
IETYLLNVSP GGLTYIAEWR GGILDHKMGH LACFSGGMIA LGAEDAKEEK RAHYRELAAQ 

       490        500        510        520        530        540 
ITKTCHESYA RSDTKLGPEA FWFNSGREAV ATQLSESYYI LRPEVVESYM YLWRQTHNPI 

       550        560        570        580        590        600 
YREWGWEVVL ALEKYCRTEA GFSGIQDVYS STPNHDNKQQ SFFLAETLKY LYLLFSEDDL 

       610        620        630 
LSLEDWVFNT EAHPLPVNHS DSSGRAWGRH 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a cDNA encoding a novel human Golgi alpha 1,2-mannosidase involved in N-glycan biosynthesis."
Tremblay L.O., Herscovics A.
J. Biol. Chem. 275:31655-31660(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain and Eye.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF261655 mRNA. Translation: AAF97058.1.
AL031280, AL020996 Genomic DNA. Translation: CAI19714.1.
BC137017 mRNA. Translation: AAI37018.1.
RefSeqNP_001275939.1. NM_001289010.1.
NP_065112.1. NM_020379.3.
UniGeneHs.197043.

3D structure databases

ProteinModelPortalQ9NR34.
SMRQ9NR34. Positions 163-617.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121395. 1 interaction.
IntActQ9NR34. 1 interaction.
MINTMINT-1187619.
STRING9606.ENSP00000363452.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

PTM databases

PhosphoSiteQ9NR34.

Polymorphism databases

DMDM17369308.

Proteomic databases

PaxDbQ9NR34.
PRIDEQ9NR34.

Protocols and materials databases

DNASU57134.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374332; ENSP00000363452; ENSG00000117643.
GeneID57134.
KEGGhsa:57134.
UCSCuc001bkm.2. human.

Organism-specific databases

CTD57134.
GeneCardsGC01P025943.
HGNCHGNC:19080. MAN1C1.
HPAHPA048352.
neXtProtNX_Q9NR34.
PharmGKBPA38788.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG300315.
HOGENOMHOG000181988.
HOVERGENHBG052389.
InParanoidQ9NR34.
KOK01230.
OMARSWGWAM.
OrthoDBEOG7X0VH1.
PhylomeDBQ9NR34.
TreeFamTF313420.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ9NR34.
BgeeQ9NR34.
CleanExHS_MAN1C1.
GenevestigatorQ9NR34.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi57134.
NextBio63051.
PROQ9NR34.

Entry information

Entry nameMA1C1_HUMAN
AccessionPrimary (citable) accession number: Q9NR34
Secondary accession number(s): A6NNE2, B2RNP2, Q9Y545
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries