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Q9NR33

- DPOE4_HUMAN

UniProt

Q9NR33 - DPOE4_HUMAN

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Protein

DNA polymerase epsilon subunit 4

Gene

POLE4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in allowing polymerase epsilon to carry out its replication and/or repair function.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA-directed DNA polymerase activity Source: ProtInc
  2. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. DNA-dependent DNA replication Source: GOC
  2. histone H3 acetylation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 4 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 4
DNA polymerase epsilon subunit p12
Gene namesi
Name:POLE4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:18755. POLE4.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
  2. epsilon DNA polymerase complex Source: UniProtKB
  3. nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38677.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 117116DNA polymerase epsilon subunit 4PRO_0000191746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine3 Publications
Modified residuei11 – 111Phosphothreonine1 Publication
Modified residuei25 – 251Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9NR33.
PaxDbiQ9NR33.
PRIDEiQ9NR33.

PTM databases

PhosphoSiteiQ9NR33.

Expressioni

Gene expression databases

BgeeiQ9NR33.
CleanExiHS_POLE4.
GenevestigatoriQ9NR33.

Organism-specific databases

HPAiHPA054979.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE3 is a prerequisite for further binding with POLE and POLE2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ORFQ9Q2G42EBI-867034,EBI-6248094From a different organism.
POLE3Q9NRF93EBI-867034,EBI-744901

Protein-protein interaction databases

BioGridi121168. 13 interactions.
IntActiQ9NR33. 6 interactions.
MINTiMINT-3072641.
STRINGi9606.ENSP00000420176.

Structurei

3D structure databases

ProteinModelPortaliQ9NR33.
SMRiQ9NR33. Positions 41-112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG5208.
GeneTreeiENSGT00530000063560.
HOGENOMiHOG000007271.
HOVERGENiHBG051399.
InParanoidiQ9NR33.
KOiK03506.
OMAiPITRIKH.
OrthoDBiEOG7BGHNS.
PhylomeDBiQ9NR33.
TreeFamiTF103009.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NR33-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAAAAGSG TPREEEGPAG EAAASQPQAP TSVPGARLSR LPLARVKALV
60 70 80 90 100
KADPDVTLAG QEAIFILARA AELFVETIAK DAYCCAQQGK RKTLQRRDLD
110
NAIEAVDEFA FLEGTLD
Length:117
Mass (Da):12,209
Last modified:October 17, 2006 - v2
Checksum:i1F7273FFEC9D318C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171G → V.3 Publications
Corresponds to variant rs12366 [ dbSNP | Ensembl ].
VAR_028050

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF261688 Genomic DNA. Translation: AAF90132.1.
AC007400 Genomic DNA. Translation: AAY15030.1.
BC031331 mRNA. Translation: AAH31331.1.
CCDSiCCDS1957.1.
RefSeqiNP_063949.2. NM_019896.2.
UniGeneiHs.469060.

Genome annotation databases

EnsembliENST00000483063; ENSP00000420176; ENSG00000115350.
GeneIDi56655.
KEGGihsa:56655.
UCSCiuc002snf.3. human.

Polymorphism databases

DMDMi116241340.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF261688 Genomic DNA. Translation: AAF90132.1 .
AC007400 Genomic DNA. Translation: AAY15030.1 .
BC031331 mRNA. Translation: AAH31331.1 .
CCDSi CCDS1957.1.
RefSeqi NP_063949.2. NM_019896.2.
UniGenei Hs.469060.

3D structure databases

ProteinModelPortali Q9NR33.
SMRi Q9NR33. Positions 41-112.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121168. 13 interactions.
IntActi Q9NR33. 6 interactions.
MINTi MINT-3072641.
STRINGi 9606.ENSP00000420176.

Chemistry

DrugBanki DB00242. Cladribine.

PTM databases

PhosphoSitei Q9NR33.

Polymorphism databases

DMDMi 116241340.

Proteomic databases

MaxQBi Q9NR33.
PaxDbi Q9NR33.
PRIDEi Q9NR33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000483063 ; ENSP00000420176 ; ENSG00000115350 .
GeneIDi 56655.
KEGGi hsa:56655.
UCSCi uc002snf.3. human.

Organism-specific databases

CTDi 56655.
GeneCardsi GC02P075185.
H-InvDB HIX0002198.
HGNCi HGNC:18755. POLE4.
HPAi HPA054979.
MIMi 607269. gene.
neXtProti NX_Q9NR33.
PharmGKBi PA38677.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5208.
GeneTreei ENSGT00530000063560.
HOGENOMi HOG000007271.
HOVERGENi HBG051399.
InParanoidi Q9NR33.
KOi K03506.
OMAi PITRIKH.
OrthoDBi EOG7BGHNS.
PhylomeDBi Q9NR33.
TreeFami TF103009.

Miscellaneous databases

GenomeRNAii 56655.
NextBioi 62121.
PROi Q9NR33.
SOURCEi Search...

Gene expression databases

Bgeei Q9NR33.
CleanExi HS_POLE4.
Genevestigatori Q9NR33.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view ]
Pfami PF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view ]
SUPFAMi SSF47113. SSF47113. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon."
    Li Y., Pursell Z.F., Linn S.
    J. Biol. Chem. 275:23247-23252(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN EPSILON DNA POLYMERASE COMPLEX, VARIANT VAL-17.
    Tissue: Cervix carcinoma.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-17.
    Tissue: Skin.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, VARIANT [LARGE SCALE ANALYSIS] VAL-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDPOE4_HUMAN
AccessioniPrimary (citable) accession number: Q9NR33
Secondary accession number(s): Q53TR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3