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Protein

DNA polymerase epsilon subunit 4

Gene

POLE4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in allowing polymerase epsilon to carry out its replication and/or repair function.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: ProtInc

GO - Biological processi

  • histone H3 acetylation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS03874-MONOMER.
ReactomeiR-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-174430. Telomere C-strand synthesis initiation.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-68952. DNA replication initiation.
R-HSA-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 4 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 4
DNA polymerase epsilon subunit p12
Gene namesi
Name:POLE4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:18755. POLE4.

Subcellular locationi

GO - Cellular componenti

  • Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
  • epsilon DNA polymerase complex Source: UniProtKB
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi56655.
OpenTargetsiENSG00000115350.
PharmGKBiPA38677.

Chemistry databases

DrugBankiDB00242. Cladribine.

Polymorphism and mutation databases

BioMutaiPOLE4.
DMDMi116241340.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001917462 – 117DNA polymerase epsilon subunit 4Add BLAST116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei11PhosphothreonineCombined sources1
Modified residuei25PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NR33.
MaxQBiQ9NR33.
PaxDbiQ9NR33.
PeptideAtlasiQ9NR33.
PRIDEiQ9NR33.
TopDownProteomicsiQ9NR33.

PTM databases

iPTMnetiQ9NR33.
PhosphoSitePlusiQ9NR33.

Expressioni

Gene expression databases

BgeeiENSG00000115350.
CleanExiHS_POLE4.
GenevisibleiQ9NR33. HS.

Organism-specific databases

HPAiHPA054979.
HPA071815.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE3 is a prerequisite for further binding with POLE and POLE2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NFYBP252089EBI-867034,EBI-389728
ORFQ9Q2G42EBI-867034,EBI-6248094From a different organism.
POLE3Q9NRF97EBI-867034,EBI-744901

Protein-protein interaction databases

BioGridi121168. 19 interactors.
IntActiQ9NR33. 9 interactors.
MINTiMINT-3072641.
STRINGi9606.ENSP00000420176.

Structurei

3D structure databases

ProteinModelPortaliQ9NR33.
SMRiQ9NR33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG1658. Eukaryota.
ENOG41122NU. LUCA.
GeneTreeiENSGT00530000063560.
HOGENOMiHOG000007271.
HOVERGENiHBG051399.
InParanoidiQ9NR33.
KOiK03506.
OMAiMHVATHE.
OrthoDBiEOG091G0HN1.
PhylomeDBiQ9NR33.
TreeFamiTF103009.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NR33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAAGSG TPREEEGPAG EAAASQPQAP TSVPGARLSR LPLARVKALV
60 70 80 90 100
KADPDVTLAG QEAIFILARA AELFVETIAK DAYCCAQQGK RKTLQRRDLD
110
NAIEAVDEFA FLEGTLD
Length:117
Mass (Da):12,209
Last modified:October 17, 2006 - v2
Checksum:i1F7273FFEC9D318C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02805017G → V.Combined sources2 PublicationsCorresponds to variant rs12366dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF261688 Genomic DNA. Translation: AAF90132.1.
AC007400 Genomic DNA. Translation: AAY15030.1.
BC031331 mRNA. Translation: AAH31331.1.
CCDSiCCDS1957.1.
RefSeqiNP_063949.2. NM_019896.2.
UniGeneiHs.469060.

Genome annotation databases

EnsembliENST00000483063; ENSP00000420176; ENSG00000115350.
GeneIDi56655.
KEGGihsa:56655.
UCSCiuc002snf.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF261688 Genomic DNA. Translation: AAF90132.1.
AC007400 Genomic DNA. Translation: AAY15030.1.
BC031331 mRNA. Translation: AAH31331.1.
CCDSiCCDS1957.1.
RefSeqiNP_063949.2. NM_019896.2.
UniGeneiHs.469060.

3D structure databases

ProteinModelPortaliQ9NR33.
SMRiQ9NR33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121168. 19 interactors.
IntActiQ9NR33. 9 interactors.
MINTiMINT-3072641.
STRINGi9606.ENSP00000420176.

Chemistry databases

DrugBankiDB00242. Cladribine.

PTM databases

iPTMnetiQ9NR33.
PhosphoSitePlusiQ9NR33.

Polymorphism and mutation databases

BioMutaiPOLE4.
DMDMi116241340.

Proteomic databases

EPDiQ9NR33.
MaxQBiQ9NR33.
PaxDbiQ9NR33.
PeptideAtlasiQ9NR33.
PRIDEiQ9NR33.
TopDownProteomicsiQ9NR33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000483063; ENSP00000420176; ENSG00000115350.
GeneIDi56655.
KEGGihsa:56655.
UCSCiuc002snf.4. human.

Organism-specific databases

CTDi56655.
DisGeNETi56655.
GeneCardsiPOLE4.
H-InvDBHIX0002198.
HGNCiHGNC:18755. POLE4.
HPAiHPA054979.
HPA071815.
MIMi607269. gene.
neXtProtiNX_Q9NR33.
OpenTargetsiENSG00000115350.
PharmGKBiPA38677.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1658. Eukaryota.
ENOG41122NU. LUCA.
GeneTreeiENSGT00530000063560.
HOGENOMiHOG000007271.
HOVERGENiHBG051399.
InParanoidiQ9NR33.
KOiK03506.
OMAiMHVATHE.
OrthoDBiEOG091G0HN1.
PhylomeDBiQ9NR33.
TreeFamiTF103009.

Enzyme and pathway databases

BioCyciZFISH:HS03874-MONOMER.
ReactomeiR-HSA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-HSA-174430. Telomere C-strand synthesis initiation.
R-HSA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-5685942. HDR through Homologous Recombination (HRR).
R-HSA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-68952. DNA replication initiation.
R-HSA-68962. Activation of the pre-replicative complex.

Miscellaneous databases

GenomeRNAii56655.
PROiQ9NR33.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115350.
CleanExiHS_POLE4.
GenevisibleiQ9NR33. HS.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE4_HUMAN
AccessioniPrimary (citable) accession number: Q9NR33
Secondary accession number(s): Q53TR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.