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Q9NR33

- DPOE4_HUMAN

UniProt

Q9NR33 - DPOE4_HUMAN

Protein

DNA polymerase epsilon subunit 4

Gene

POLE4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May play a role in allowing polymerase epsilon to carry out its replication and/or repair function.

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA-directed DNA polymerase activity Source: ProtInc
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA-dependent DNA replication Source: GOC
    2. histone H3 acetylation Source: BHF-UCL

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase epsilon subunit 4 (EC:2.7.7.7)
    Alternative name(s):
    DNA polymerase II subunit 4
    DNA polymerase epsilon subunit p12
    Gene namesi
    Name:POLE4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:18755. POLE4.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
    2. epsilon DNA polymerase complex Source: UniProtKB
    3. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38677.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 117116DNA polymerase epsilon subunit 4PRO_0000191746Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine3 Publications
    Modified residuei11 – 111Phosphothreonine1 Publication
    Modified residuei25 – 251Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9NR33.
    PaxDbiQ9NR33.
    PRIDEiQ9NR33.

    PTM databases

    PhosphoSiteiQ9NR33.

    Expressioni

    Gene expression databases

    BgeeiQ9NR33.
    CleanExiHS_POLE4.
    GenevestigatoriQ9NR33.

    Organism-specific databases

    HPAiHPA054979.

    Interactioni

    Subunit structurei

    Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE3 is a prerequisite for further binding with POLE and POLE2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ORFQ9Q2G42EBI-867034,EBI-6248094From a different organism.
    POLE3Q9NRF93EBI-867034,EBI-744901

    Protein-protein interaction databases

    BioGridi121168. 12 interactions.
    IntActiQ9NR33. 6 interactions.
    MINTiMINT-3072641.
    STRINGi9606.ENSP00000420176.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NR33.
    SMRiQ9NR33. Positions 41-112.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG5208.
    HOGENOMiHOG000007271.
    HOVERGENiHBG051399.
    KOiK03506.
    OMAiPITRIKH.
    OrthoDBiEOG7BGHNS.
    PhylomeDBiQ9NR33.
    TreeFamiTF103009.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR003958. CBFA_NFYB_domain.
    IPR009072. Histone-fold.
    [Graphical view]
    PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9NR33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAAAAGSG TPREEEGPAG EAAASQPQAP TSVPGARLSR LPLARVKALV    50
    KADPDVTLAG QEAIFILARA AELFVETIAK DAYCCAQQGK RKTLQRRDLD 100
    NAIEAVDEFA FLEGTLD 117
    Length:117
    Mass (Da):12,209
    Last modified:October 17, 2006 - v2
    Checksum:i1F7273FFEC9D318C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti17 – 171G → V.3 Publications
    Corresponds to variant rs12366 [ dbSNP | Ensembl ].
    VAR_028050

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF261688 Genomic DNA. Translation: AAF90132.1.
    AC007400 Genomic DNA. Translation: AAY15030.1.
    BC031331 mRNA. Translation: AAH31331.1.
    CCDSiCCDS1957.1.
    RefSeqiNP_063949.2. NM_019896.2.
    UniGeneiHs.469060.

    Genome annotation databases

    EnsembliENST00000483063; ENSP00000420176; ENSG00000115350.
    GeneIDi56655.
    KEGGihsa:56655.
    UCSCiuc002snf.3. human.

    Polymorphism databases

    DMDMi116241340.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF261688 Genomic DNA. Translation: AAF90132.1 .
    AC007400 Genomic DNA. Translation: AAY15030.1 .
    BC031331 mRNA. Translation: AAH31331.1 .
    CCDSi CCDS1957.1.
    RefSeqi NP_063949.2. NM_019896.2.
    UniGenei Hs.469060.

    3D structure databases

    ProteinModelPortali Q9NR33.
    SMRi Q9NR33. Positions 41-112.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121168. 12 interactions.
    IntActi Q9NR33. 6 interactions.
    MINTi MINT-3072641.
    STRINGi 9606.ENSP00000420176.

    PTM databases

    PhosphoSitei Q9NR33.

    Polymorphism databases

    DMDMi 116241340.

    Proteomic databases

    MaxQBi Q9NR33.
    PaxDbi Q9NR33.
    PRIDEi Q9NR33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000483063 ; ENSP00000420176 ; ENSG00000115350 .
    GeneIDi 56655.
    KEGGi hsa:56655.
    UCSCi uc002snf.3. human.

    Organism-specific databases

    CTDi 56655.
    GeneCardsi GC02P075185.
    H-InvDB HIX0002198.
    HGNCi HGNC:18755. POLE4.
    HPAi HPA054979.
    MIMi 607269. gene.
    neXtProti NX_Q9NR33.
    PharmGKBi PA38677.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5208.
    HOGENOMi HOG000007271.
    HOVERGENi HBG051399.
    KOi K03506.
    OMAi PITRIKH.
    OrthoDBi EOG7BGHNS.
    PhylomeDBi Q9NR33.
    TreeFami TF103009.

    Miscellaneous databases

    GenomeRNAii 56655.
    NextBioi 62121.
    PROi Q9NR33.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9NR33.
    CleanExi HS_POLE4.
    Genevestigatori Q9NR33.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR003958. CBFA_NFYB_domain.
    IPR009072. Histone-fold.
    [Graphical view ]
    Pfami PF00808. CBFD_NFYB_HMF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon."
      Li Y., Pursell Z.F., Linn S.
      J. Biol. Chem. 275:23247-23252(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN EPSILON DNA POLYMERASE COMPLEX, VARIANT VAL-17.
      Tissue: Cervix carcinoma.
    2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-17.
      Tissue: Skin.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, VARIANT [LARGE SCALE ANALYSIS] VAL-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDPOE4_HUMAN
    AccessioniPrimary (citable) accession number: Q9NR33
    Secondary accession number(s): Q53TR2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3