Reviewed, UniProtKB/Swiss-Prot Q9NR20 (DYRK4_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity tyrosine-phosphorylation-regulated kinase 4
    EC=2.7.12.1

Gene names

Name:

DYRK4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible non-essential role in spermiogenesis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm. Ref.5
Post-translational modification	Autophosphorylated on tyrosine residues By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Non-traceable author statement. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Non-traceable author statement. Source: UniProtKB protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NR20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Isoform 2 (identifier: Q9NR20-2)

The sequence of this isoform differs from the canonical sequence as follows:
     441-441: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Dual specificity tyrosine-phosphorylation-regulated kinase 4		PRO_0000085940
Regions
	Domain	104 – 400	297	Protein kinase
	Nucleotide binding	110 – 118	9	ATP By similarity
Sites
	Active site	230	1	Proton acceptor By similarity
	Binding site	133	1	ATP
Amino acid modifications
	Modified residue	264	1	Phosphotyrosine Ref.4
Natural variations
	Alternative sequence	441	1	Missing in isoform 2.		VSP_013745
	Natural variant	61	1	A → T: dbSNP rs12306130.		VAR_033900
	Natural variant	70	1	A → S Ref.6		VAR_040465
	Natural variant	95	1	V → I Ref.1		VAR_010721
	Natural variant	189	1	N → S: dbSNP rs3741927. Ref.1		VAR_010722
	Natural variant	454	1	D → V: dbSNP rs1801016.		VAR_014948
Experimental info
	Mutagenesis	133	1	K → R: Loss of kinase activity. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 33FECD8249DDB223	FASTA	520	59,608
	10 20 30 40 50 60 MPASELKASE IPFHPSIKTQ DPKAEEKSPK KQKVTLTAAE ALKLFKNQLS PYEQSEILGY 70 80 90 100 110 120 AELWFLGLEA KKLDTAPEKF SKTSFDDEHG FYLKVLHDHI AYRYEVLETI GKGSFGQVAK 130 140 150 160 170 180 CLDHKNNELV ALKIIRNKKR FHQQALMELK ILEALRKKDK DNTYNVVHMK DFFYFRNHFC 190 200 210 220 230 240 ITFELLGINL YELMKNNNFQ GFSLSIVRRF TLSVLKCLQM LSVEKIIHCD LKPENIVLYQ 250 260 270 280 290 300 KGQASVKVID FGSSCYEHQK VYTYIQSRFY RSPEVILGHP YDVAIDMWSL GCITAELYTG 310 320 330 340 350 360 YPLFPGENEV EQLACIMEVL GLPPAGFIQT ASRRQTFFDS KGFPKNITNN RGKKRYPDSK 370 380 390 400 410 420 DLTMVLKTYD TSFLDFLRRC LVWEPSLRMT PDQALKHAWI HQSRNLKPQP RPQTLRKSNS 430 440 450 460 470 480 FFPSETRKDK VQGCHHSSRK ADEITKETTE KTKDSPTKHV QHSGDQQDCL QHGADTVQLP 490 500 510 520 QLVDAPKKSE AAVGAEVSMT SPGQSKNFSL KNTNVLPPIV
	Isoform 2. Checksum: 2ACD1BC87AA1591B	FASTA	519	59,537
	10 20 30 40 50 60 MPASELKASE IPFHPSIKTQ DPKAEEKSPK KQKVTLTAAE ALKLFKNQLS PYEQSEILGY 70 80 90 100 110 120 AELWFLGLEA KKLDTAPEKF SKTSFDDEHG FYLKVLHDHI AYRYEVLETI GKGSFGQVAK 130 140 150 160 170 180 CLDHKNNELV ALKIIRNKKR FHQQALMELK ILEALRKKDK DNTYNVVHMK DFFYFRNHFC 190 200 210 220 230 240 ITFELLGINL YELMKNNNFQ GFSLSIVRRF TLSVLKCLQM LSVEKIIHCD LKPENIVLYQ 250 260 270 280 290 300 KGQASVKVID FGSSCYEHQK VYTYIQSRFY RSPEVILGHP YDVAIDMWSL GCITAELYTG 310 320 330 340 350 360 YPLFPGENEV EQLACIMEVL GLPPAGFIQT ASRRQTFFDS KGFPKNITNN RGKKRYPDSK 370 380 390 400 410 420 DLTMVLKTYD TSFLDFLRRC LVWEPSLRMT PDQALKHAWI HQSRNLKPQP RPQTLRKSNS 430 440 450 460 470 480 FFPSETRKDK VQGCHHSSRK DEITKETTEK TKDSPTKHVQ HSGDQQDCLQ HGADTVQLPQ 490 500 510 LVDAPKKSEA AVGAEVSMTS PGQSKNFSLK NTNVLPPIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Autosomal dominant hypophosphataemic rickets is associated with mutations in FGF23." White K.E., Evans W.E., O'Riordan J.L.H., Speer M.C., Econs M.J., Lorenz-Depiereux B., Grabowski M., Meitinger T., Strom T.M. Nat. Genet. 26:345-348(2000) [PubMed: 11062477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANTS ILE-95 AND SER-189. Tissue: Brain and Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases." Becker W., Weber Y., Wetzel K., Eirmbter K., Tejedor F.J., Joost H.-G. J. Biol. Chem. 273:25893-25902(1998) [PubMed: 9748265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 280-520 (ISOFORM 2). Tissue: Lung.
[4]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-264, MASS SPECTROMETRY.
[5]	"The expression of the testis-specific Dyrk4 kinase is highly restricted to step 8 spermatids but is not required for male fertility in mice." Sacher F., Moeller C., Bone W., Gottwald U., Fritsch M. Mol. Cell. Endocrinol. 267:80-88(2007) [PubMed: 17292540] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-133.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF263541 mRNA. Translation: AAF91393.1. Different initiation. BC031244 mRNA. Translation: AAH31244.1. Y09305 mRNA. Translation: CAA70488.1.
IPI	IPI00396286. IPI00555676.
RefSeq	NP_003836.1.
UniGene	Hs.439530
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
ModBase	Search...
PTM databases
PhosphoSite	Q9NR20.
Proteomic databases
PRIDE	Q9NR20.
Genome annotation databases
Ensembl	ENSG00000010219. Homo sapiens. [Contig view]
GeneID	8798.
KEGG	hsa:8798.
Organism-specific databases
GeneCards	GC12P004569.
H-InvDB	HIX0010350.
HGNC	HGNC:3095. DYRK4.
MIM	609181. gene.
PharmGKB	PA27552.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NR20.
HOVERGEN	Q9NR20.
OMA	Q9NR20. LYEQSEI.
Enzyme and pathway databases
BRENDA	2.7.12.1. 247.
Gene expression databases
Bgee	Q9NR20.
CleanEx	HS_DYRK4.
GermOnline	ENSG00000010219. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	33000.
SOURCE	Search...

Entry information

Entry name

DYRK4_HUMAN

Accession

Primary (citable) accession number: Q9NR20
Secondary accession number(s): Q8NEF2, Q92631

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 10, 2005
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)