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Protein

Dual specificity tyrosine-phosphorylation-regulated kinase 4

Gene

DYRK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible non-essential role in spermiogenesis.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133ATP1
Active sitei230Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi110 – 118ATPPROSITE-ProRule annotation9
Nucleotide bindingi183 – 186ATPPROSITE-ProRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00275-MONOMER.
SignaLinkiQ9NR20.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity tyrosine-phosphorylation-regulated kinase 4 (EC:2.7.12.1)
Gene namesi
Name:DYRK4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:3095. DYRK4.

Subcellular locationi

Isoform 1 :
Isoform 4 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133K → R: Loss of kinase activity. 1 Publication1
Mutagenesisi264Y → F: Abolishes kinase activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000010219.
PharmGKBiPA27552.

Chemistry databases

ChEMBLiCHEMBL1075115.
GuidetoPHARMACOLOGYi2013.

Polymorphism and mutation databases

BioMutaiDYRK4.
DMDMi68566308.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859401 – 520Dual specificity tyrosine-phosphorylation-regulated kinase 4Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei264Phosphotyrosine; by autocatalysis1 Publication1

Post-translational modificationi

Autophosphorylation on Tyr-264 in the activation loop is required for kinase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NR20.
MaxQBiQ9NR20.
PaxDbiQ9NR20.
PeptideAtlasiQ9NR20.
PRIDEiQ9NR20.

PTM databases

iPTMnetiQ9NR20.
PhosphoSitePlusiQ9NR20.

Expressioni

Gene expression databases

BgeeiENSG00000010219.
CleanExiHS_DYRK4.
ExpressionAtlasiQ9NR20. baseline and differential.
GenevisibleiQ9NR20. HS.

Organism-specific databases

HPAiHPA028065.
HPA056073.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DYRK2Q926303EBI-3914009,EBI-749432
MID1O153443EBI-3914009,EBI-2340316

Protein-protein interaction databases

BioGridi114326. 16 interactors.
IntActiQ9NR20. 13 interactors.
STRINGi9606.ENSP00000010132.

Chemistry databases

BindingDBiQ9NR20.

Structurei

3D structure databases

ProteinModelPortaliQ9NR20.
SMRiQ9NR20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 400Protein kinasePROSITE-ProRule annotationAdd BLAST297

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
GeneTreeiENSGT00760000119032.
HOGENOMiHOG000220863.
HOVERGENiHBG051426.
InParanoidiQ9NR20.
KOiK18669.
PhylomeDBiQ9NR20.
TreeFamiTF314624.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NR20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPASELKASE IPFHPSIKTQ DPKAEEKSPK KQKVTLTAAE ALKLFKNQLS
60 70 80 90 100
PYEQSEILGY AELWFLGLEA KKLDTAPEKF SKTSFDDEHG FYLKVLHDHI
110 120 130 140 150
AYRYEVLETI GKGSFGQVAK CLDHKNNELV ALKIIRNKKR FHQQALMELK
160 170 180 190 200
ILEALRKKDK DNTYNVVHMK DFFYFRNHFC ITFELLGINL YELMKNNNFQ
210 220 230 240 250
GFSLSIVRRF TLSVLKCLQM LSVEKIIHCD LKPENIVLYQ KGQASVKVID
260 270 280 290 300
FGSSCYEHQK VYTYIQSRFY RSPEVILGHP YDVAIDMWSL GCITAELYTG
310 320 330 340 350
YPLFPGENEV EQLACIMEVL GLPPAGFIQT ASRRQTFFDS KGFPKNITNN
360 370 380 390 400
RGKKRYPDSK DLTMVLKTYD TSFLDFLRRC LVWEPSLRMT PDQALKHAWI
410 420 430 440 450
HQSRNLKPQP RPQTLRKSNS FFPSETRKDK VQGCHHSSRK ADEITKETTE
460 470 480 490 500
KTKDSPTKHV QHSGDQQDCL QHGADTVQLP QLVDAPKKSE AAVGAEVSMT
510 520
SPGQSKNFSL KNTNVLPPIV
Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Length:520
Mass (Da):59,608
Last modified:May 10, 2005 - v2
Checksum:i33FECD8249DDB223
GO
Isoform 2 (identifier: Q9NR20-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     441-441: Missing.

Show »
Length:519
Mass (Da):59,537
Checksum:i2ACD1BC87AA1591B
GO
Isoform 3 (identifier: Q9NR20-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MQLLPPPIRT...YQENQAHNQM

Note: No experimental confirmation available.
Show »
Length:635
Mass (Da):72,577
Checksum:i42F432C350F65F2D
GO
Isoform 4 (identifier: Q9NR20-4) [UniParc]FASTAAdd to basket
Also known as: DYRK4-L

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MQLLPPPIRT...YQENQAHNQM

Note: Contains a bipartite nuclear localization signal at positions 19-37.1 Publication
Show »
Length:644
Mass (Da):73,569
Checksum:i688041E86A28626C
GO
Isoform 5 (identifier: Q9NR20-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     380-382: Missing.

Note: Due to an alternative splicing donor site in exon 19. Markedly reduced enzymatic activity.1 Publication
Show »
Length:517
Mass (Da):59,293
Checksum:i0C076681A026AC27
GO

Sequence cautioni

The sequence AAF91393 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AK308260 differs from that shown. Reason: Frameshift at position 470.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165N → I in BAF85011 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03390061A → T.Corresponds to variant rs12306130dbSNPEnsembl.1
Natural variantiVAR_04046570A → S.1 Publication1
Natural variantiVAR_01072195V → I.1 PublicationCorresponds to variant rs746486416dbSNPEnsembl.1
Natural variantiVAR_010722189N → S.1 PublicationCorresponds to variant rs3741927dbSNPEnsembl.1
Natural variantiVAR_014948454D → V.Corresponds to variant rs1801016dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0571321M → MQLLPPPIRTGTKTQMDAKK PRKCDLTPFLVLKARKKQKF TSAKVGSKLSVQIQKPPSNI KNSRMTQVFHKNTSVTSLPF VDTKGKKNTVSFPHISKKVL LKSSLLYQENQAHNQM in isoform 3. 1 Publication1
Alternative sequenceiVSP_0571331M → MQLLPPPIRTGTKTQMDAKK PRKCDLTPFLVLKARKKQKF TSAKGPTLSEIYMVGSKLSV QIQKPPSNIKNSRMTQVFHK NTSVTSLPFVDTKGKKNTVS FPHISKKVLLKSSLLYQENQ AHNQM in isoform 4. 1 Publication1
Alternative sequenceiVSP_057134380 – 382Missing in isoform 5. 1 Publication3
Alternative sequenceiVSP_013745441Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263541 mRNA. Translation: AAF91393.1. Different initiation.
AK292322 mRNA. Translation: BAF85011.1.
AK308260 mRNA. No translation available.
BC031244 mRNA. Translation: AAH31244.1.
Y09305 mRNA. Translation: CAA70488.1.
CCDSiCCDS8530.1. [Q9NR20-1]
RefSeqiNP_003836.1. NM_003845.2. [Q9NR20-1]
UniGeneiHs.439530.

Genome annotation databases

EnsembliENST00000010132; ENSP00000010132; ENSG00000010219. [Q9NR20-1]
ENST00000540757; ENSP00000441755; ENSG00000010219. [Q9NR20-1]
GeneIDi8798.
KEGGihsa:8798.
UCSCiuc001qmx.5. human. [Q9NR20-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263541 mRNA. Translation: AAF91393.1. Different initiation.
AK292322 mRNA. Translation: BAF85011.1.
AK308260 mRNA. No translation available.
BC031244 mRNA. Translation: AAH31244.1.
Y09305 mRNA. Translation: CAA70488.1.
CCDSiCCDS8530.1. [Q9NR20-1]
RefSeqiNP_003836.1. NM_003845.2. [Q9NR20-1]
UniGeneiHs.439530.

3D structure databases

ProteinModelPortaliQ9NR20.
SMRiQ9NR20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114326. 16 interactors.
IntActiQ9NR20. 13 interactors.
STRINGi9606.ENSP00000010132.

Chemistry databases

BindingDBiQ9NR20.
ChEMBLiCHEMBL1075115.
GuidetoPHARMACOLOGYi2013.

PTM databases

iPTMnetiQ9NR20.
PhosphoSitePlusiQ9NR20.

Polymorphism and mutation databases

BioMutaiDYRK4.
DMDMi68566308.

Proteomic databases

EPDiQ9NR20.
MaxQBiQ9NR20.
PaxDbiQ9NR20.
PeptideAtlasiQ9NR20.
PRIDEiQ9NR20.

Protocols and materials databases

DNASUi8798.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000010132; ENSP00000010132; ENSG00000010219. [Q9NR20-1]
ENST00000540757; ENSP00000441755; ENSG00000010219. [Q9NR20-1]
GeneIDi8798.
KEGGihsa:8798.
UCSCiuc001qmx.5. human. [Q9NR20-1]

Organism-specific databases

CTDi8798.
GeneCardsiDYRK4.
HGNCiHGNC:3095. DYRK4.
HPAiHPA028065.
HPA056073.
MIMi609181. gene.
neXtProtiNX_Q9NR20.
OpenTargetsiENSG00000010219.
PharmGKBiPA27552.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
GeneTreeiENSGT00760000119032.
HOGENOMiHOG000220863.
HOVERGENiHBG051426.
InParanoidiQ9NR20.
KOiK18669.
PhylomeDBiQ9NR20.
TreeFamiTF314624.

Enzyme and pathway databases

BioCyciZFISH:HS00275-MONOMER.
SignaLinkiQ9NR20.

Miscellaneous databases

GenomeRNAii8798.
PROiQ9NR20.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000010219.
CleanExiHS_DYRK4.
ExpressionAtlasiQ9NR20. baseline and differential.
GenevisibleiQ9NR20. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYRK4_HUMAN
AccessioniPrimary (citable) accession number: Q9NR20
Secondary accession number(s): A8K8F7, Q8NEF2, Q92631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.