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Protein

Acetyl-coenzyme A synthetase, cytoplasmic

Gene

ACSS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates acetate so that it can be used for lipid synthesis or for energy generation.

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei363Coenzyme ABy similarity1
Binding sitei552ATPBy similarity1
Binding sitei567ATPBy similarity1
Binding sitei575Coenzyme A; via carbonyl oxygenBy similarity1
Binding sitei636Coenzyme ABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi439 – 441ATPBy similarity3
Nucleotide bindingi463 – 468ATPBy similarity6

GO - Molecular functioni

  • acetate-CoA ligase activity Source: UniProtKB
  • AMP binding Source: UniProtKB
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05484-MONOMER.
ZFISH:HS05484-MONOMER.
BRENDAi6.2.1.1. 2681.
ReactomeiR-HSA-71384. Ethanol oxidation.

Chemistry databases

SwissLipidsiSLP:000001167.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase, cytoplasmic (EC:6.2.1.1)
Alternative name(s):
Acetate--CoA ligase
Acetyl-CoA synthetase
Short name:
ACS
Short name:
AceCS
Acyl-CoA synthetase short-chain family member 2
Acyl-activating enzyme
Gene namesi
Name:ACSS2
Synonyms:ACAS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15814. ACSS2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55902.
OpenTargetsiENSG00000131069.
PharmGKBiPA24429.

Chemistry databases

DrugBankiDB00131. Adenosine monophosphate.
DB00171. Adenosine triphosphate.

Polymorphism and mutation databases

BioMutaiACSS2.
DMDMi20137525.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002084231 – 701Acetyl-coenzyme A synthetase, cytoplasmicAdd BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Modified residuei36PhosphoserineCombined sources1
Modified residuei263PhosphoserineBy similarity1
Modified residuei265PhosphoserineBy similarity1
Modified residuei267PhosphoserineCombined sources1
Modified residuei418N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NR19.
MaxQBiQ9NR19.
PaxDbiQ9NR19.
PeptideAtlasiQ9NR19.
PRIDEiQ9NR19.

PTM databases

iPTMnetiQ9NR19.
PhosphoSitePlusiQ9NR19.

Expressioni

Gene expression databases

BgeeiENSG00000131069.
CleanExiHS_ACSS2.
ExpressionAtlasiQ9NR19. baseline and differential.
GenevisibleiQ9NR19. HS.

Organism-specific databases

HPAiHPA004141.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi120989. 6 interactors.
IntActiQ9NR19. 5 interactors.
STRINGi9606.ENSP00000253382.

Structurei

3D structure databases

ProteinModelPortaliQ9NR19.
SMRiQ9NR19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 222Coenzyme A bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229981.
HOVERGENiHBG014401.
InParanoidiQ9NR19.
KOiK01895.
OMAiEGAPNWP.
OrthoDBiEOG091G039C.
PhylomeDBiQ9NR19.
TreeFamiTF300417.

Family and domain databases

CDDicd05966. ACS. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NR19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLPEERVRS GSGSRGQEEA GAGGRARSWS PPPEVSRSAH VPSLQRYREL
60 70 80 90 100
HRRSVEEPRE FWGDIAKEFY WKTPCPGPFL RYNFDVTKGK IFIEWMKGAT
110 120 130 140 150
TNICYNVLDR NVHEKKLGDK VAFYWEGNEP GETTQITYHQ LLVQVCQFSN
160 170 180 190 200
VLRKQGIQKG DRVAIYMPMI PELVVAMLAC ARIGALHSIV FAGFSSESLC
210 220 230 240 250
ERILDSSCSL LITTDAFYRG EKLVNLKELA DEALQKCQEK GFPVRCCIVV
260 270 280 290 300
KHLGRAELGM GDSTSQSPPI KRSCPDVQIS WNQGIDLWWH ELMQEAGDEC
310 320 330 340 350
EPEWCDAEDP LFILYTSGST GKPKGVVHTV GGYMLYVATT FKYVFDFHAE
360 370 380 390 400
DVFWCTADIG WITGHSYVTY GPLANGATSV LFEGIPTYPD VNRLWSIVDK
410 420 430 440 450
YKVTKFYTAP TAIRLLMKFG DEPVTKHSRA SLQVLGTVGE PINPEAWLWY
460 470 480 490 500
HRVVGAQRCP IVDTFWQTET GGHMLTPLPG ATPMKPGSAT FPFFGVAPAI
510 520 530 540 550
LNESGEELEG EAEGYLVFKQ PWPGIMRTVY GNHERFETTY FKKFPGYYVT
560 570 580 590 600
GDGCQRDQDG YYWITGRIDD MLNVSGHLLS TAEVESALVE HEAVAEAAVV
610 620 630 640 650
GHPHPVKGEC LYCFVTLCDG HTFSPKLTEE LKKQIREKIG PIATPDYIQN
660 670 680 690 700
APGLPKTRSG KIMRRVLRKI AQNDHDLGDM STVADPSVIS HLFSHRCLTI

Q
Length:701
Mass (Da):78,580
Last modified:October 1, 2000 - v1
Checksum:i833580B41B73A8B4
GO
Isoform 2 (identifier: Q9NR19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     277-277: V → VQGKLKEKSKRVQP

Note: No experimental confirmation available.
Show »
Length:714
Mass (Da):80,087
Checksum:i820E84E62A72E214
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79F → L in BAC03849 (PubMed:14702039).Curated1
Sequence conflicti615V → F in AAH12172 (PubMed:15489334).Curated1
Sequence conflicti680M → L in BAC03849 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046376277V → VQGKLKEKSKRVQP in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263614 mRNA. Translation: AAF75064.1.
AK092281 mRNA. Translation: BAC03849.1.
AL133324, AL049709 Genomic DNA. Translation: CAI19311.1.
AL133324, AL049709 Genomic DNA. Translation: CAI19312.1.
AL049709, AL133324 Genomic DNA. Translation: CAI19725.1.
AL049709, AL133324 Genomic DNA. Translation: CAI19726.1.
CH471077 Genomic DNA. Translation: EAW76248.1.
BC012172 mRNA. Translation: AAH12172.1.
CCDSiCCDS13243.1. [Q9NR19-1]
CCDS42868.2. [Q9NR19-2]
RefSeqiNP_001070020.2. NM_001076552.2. [Q9NR19-2]
NP_061147.1. NM_018677.3. [Q9NR19-1]
UniGeneiHs.517034.

Genome annotation databases

EnsembliENST00000253382; ENSP00000253382; ENSG00000131069. [Q9NR19-2]
ENST00000360596; ENSP00000353804; ENSG00000131069. [Q9NR19-1]
GeneIDi55902.
KEGGihsa:55902.
UCSCiuc002xbd.3. human. [Q9NR19-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263614 mRNA. Translation: AAF75064.1.
AK092281 mRNA. Translation: BAC03849.1.
AL133324, AL049709 Genomic DNA. Translation: CAI19311.1.
AL133324, AL049709 Genomic DNA. Translation: CAI19312.1.
AL049709, AL133324 Genomic DNA. Translation: CAI19725.1.
AL049709, AL133324 Genomic DNA. Translation: CAI19726.1.
CH471077 Genomic DNA. Translation: EAW76248.1.
BC012172 mRNA. Translation: AAH12172.1.
CCDSiCCDS13243.1. [Q9NR19-1]
CCDS42868.2. [Q9NR19-2]
RefSeqiNP_001070020.2. NM_001076552.2. [Q9NR19-2]
NP_061147.1. NM_018677.3. [Q9NR19-1]
UniGeneiHs.517034.

3D structure databases

ProteinModelPortaliQ9NR19.
SMRiQ9NR19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120989. 6 interactors.
IntActiQ9NR19. 5 interactors.
STRINGi9606.ENSP00000253382.

Chemistry databases

DrugBankiDB00131. Adenosine monophosphate.
DB00171. Adenosine triphosphate.
SwissLipidsiSLP:000001167.

PTM databases

iPTMnetiQ9NR19.
PhosphoSitePlusiQ9NR19.

Polymorphism and mutation databases

BioMutaiACSS2.
DMDMi20137525.

Proteomic databases

EPDiQ9NR19.
MaxQBiQ9NR19.
PaxDbiQ9NR19.
PeptideAtlasiQ9NR19.
PRIDEiQ9NR19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253382; ENSP00000253382; ENSG00000131069. [Q9NR19-2]
ENST00000360596; ENSP00000353804; ENSG00000131069. [Q9NR19-1]
GeneIDi55902.
KEGGihsa:55902.
UCSCiuc002xbd.3. human. [Q9NR19-1]

Organism-specific databases

CTDi55902.
DisGeNETi55902.
GeneCardsiACSS2.
HGNCiHGNC:15814. ACSS2.
HPAiHPA004141.
MIMi605832. gene.
neXtProtiNX_Q9NR19.
OpenTargetsiENSG00000131069.
PharmGKBiPA24429.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229981.
HOVERGENiHBG014401.
InParanoidiQ9NR19.
KOiK01895.
OMAiEGAPNWP.
OrthoDBiEOG091G039C.
PhylomeDBiQ9NR19.
TreeFamiTF300417.

Enzyme and pathway databases

BioCyciMetaCyc:HS05484-MONOMER.
ZFISH:HS05484-MONOMER.
BRENDAi6.2.1.1. 2681.
ReactomeiR-HSA-71384. Ethanol oxidation.

Miscellaneous databases

ChiTaRSiACSS2. human.
GeneWikiiACSS2.
GenomeRNAii55902.
PROiQ9NR19.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131069.
CleanExiHS_ACSS2.
ExpressionAtlasiQ9NR19. baseline and differential.
GenevisibleiQ9NR19. HS.

Family and domain databases

CDDicd05966. ACS. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSA_HUMAN
AccessioniPrimary (citable) accession number: Q9NR19
Secondary accession number(s): A6NE90
, Q5QPH2, Q5QPH3, Q8N238, Q96EL0, Q9NQP7, Q9UJ15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.