Q9NQY0 (BIN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bridging integrator 3

Gene names

Name:

BIN3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cytokinesis and septation where it has a role in the localization of F-actin.
Subcellular location	Cytoplasm › cytoskeleton.
Tissue specificity	Ubiquitously expressed except in brain. Ref.1
Sequence similarities	Contains 1 BAR domain.
Sequence caution	The sequence AAH01223.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Septation
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Non-traceable author statement Ref.1. Source: UniProtKB barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis Non-traceable author statement Ref.1. Source: UniProtKB protein localization Inferred from mutant phenotype Ref.1. Source: UniProtKB unidimensional cell growth Inferred from mutant phenotype Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cytoskeletal adaptor activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DNMBP	Q6XZF7	2	EBI-2653038,EBI-2483419

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQY0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQY0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Bridging integrator 3		PRO_0000192955
Regions
	Domain	9 – 232	224	BAR
	Coiled coil	18 – 51	34	Potential
	Coiled coil	120 – 152	33	Potential
	Coiled coil	231 – 247	17	Potential
Natural variations
	Alternative sequence	1 – 54	54	Missing in isoform 2.		VSP_013465
Experimental info
	Sequence conflict	122	1	N → I in BAA91603. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 254CC7113749C584	FASTA	253	29,665
	10 20 30 40 50 60 MSWIPFKIGQ PKKQIVPKTV ERDFEREYGK LQQLEEQTRR LQKDMKKSTD ADLAMSKSAV 70 80 90 100 110 120 KISLDLLSNP LCEQDQDLLN MVTALDTAMK RMDAFNQEKV NQIQKTVIEP LKKFGSVFPS 130 140 150 160 170 180 LNMAVKRREQ ALQDYRRLQA KVEKYEEKEK TGPVLAKLHQ AREELRPVRE DFEAKNRQLL 190 200 210 220 230 240 EEMPRFYGSR LDYFQPSFES LIRAQVVYYS EMHKIFGDLS HQLDQPGHSD EQRERENEAK 250 LSELRALSIV ADD
	Isoform 2 [UniParc]. Checksum: F3DE198DC4AB1EF0	FASTA	199	23,218
	10 20 30 40 50 60 MSKSAVKISL DLLSNPLCEQ DQDLLNMVTA LDTAMKRMDA FNQEKVNQIQ KTVIEPLKKF 70 80 90 100 110 120 GSVFPSLNMA VKRREQALQD YRRLQAKVEK YEEKEKTGPV LAKLHQAREE LRPVREDFEA 130 140 150 160 170 180 KNRQLLEEMP RFYGSRLDYF QPSFESLIRA QVVYYSEMHK IFGDLSHQLD QPGHSDEQRE 190 RENEAKLSEL RALSIVADD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human BIN3 complements the F-actin localization defects caused by loss of Hob3p, the fission yeast homolog of Rvs161p." Routhier E.L., Burn T.C., Abbaszade I., Summers M., Albright C.F., Prendergast G.C. J. Biol. Chem. 276:21670-21677(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Skin.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF271732 mRNA. Translation: AAF76218.1. AK001289 mRNA. Translation: BAA91603.1. AK023980 mRNA. Translation: BAB14751.1. BC001223 mRNA. Translation: AAH01223.1. Different initiation. BC009824 mRNA. Translation: AAH09824.1.
IPI	IPI00032966. IPI00556562.
RefSeq	NP_061158.1. NM_018688.4.
UniGene	Hs.654720.
3D structure databases
ProteinModelPortal	Q9NQY0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NQY0. 1 interaction.
STRING	9606.ENSP00000276416.
PTM databases
PhosphoSite	Q9NQY0.
Polymorphism databases
DMDM	30912742.
Proteomic databases
PaxDb	Q9NQY0.
PRIDE	Q9NQY0.
Protocols and materials databases
DNASU	55909.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276416; ENSP00000276416; ENSG00000147439. ENST00000519513; ENSP00000430423; ENSG00000147439.
GeneID	55909.
KEGG	hsa:55909.
UCSC	uc003xcl.3. human.
Organism-specific databases
CTD	55909.
GeneCards	GC08M022477.
HGNC	HGNC:1054. BIN3.
HPA	HPA023769.
MIM	606396. gene.
neXtProt	NX_Q9NQY0.
PharmGKB	PA25357.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG264615.
HOGENOM	HOG000007254.
HOVERGEN	HBG001358.
InParanoid	Q9NQY0.
OMA	VVYYTEM.
OrthoDB	EOG4T1HNB.
PhylomeDB	Q9NQY0.
Gene expression databases
ArrayExpress	Q9NQY0.
Bgee	Q9NQY0.
CleanEx	HS_BIN3.
Genevestigator	Q9NQY0.
GermOnline	ENSG00000147439. Homo sapiens.
Family and domain databases
Gene3D	1.20.1270.60. 1 hit.
InterPro	IPR027267. AH/BAR-dom. IPR004148. BAR_dom. [Graphical view]
Pfam	PF03114. BAR. 1 hit. [Graphical view]
SMART	SM00721. BAR. 1 hit. [Graphical view]
PROSITE	PS51021. BAR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55909.
NextBio	61298.
SOURCE	Search...

Entry information

Entry name

BIN3_HUMAN

Accession

Primary (citable) accession number: Q9NQY0
Secondary accession number(s): Q9BVG2, Q9NVY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.