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Q9NQW1 (SC31B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein Sec31B
Alternative name(s):
SEC31-like protein 2
SEC31-related protein B
SEC31B-1
Gene names
Name:SEC31B
Synonyms:SEC31L2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1179 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

As a component of the coat protein complex II (COPII), may function in vesicle budding and cargo export from the endoplasmic reticulum. Ref.1

Subunit structure

COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13 By similarity. Interacts with SEC31A. Ref.1

Subcellular location

Cytoplasm. Cytoplasmic vesicleCOPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein Ref.1.

Tissue specificity

Ubiquitously expressed at low levels with specific expression in thymus and testis. Expressed in testis by Sertoli cells, Leydig cells and spermatogonia and in cerebellum more prominently by Purkinje and granular cells (at protein level). Ref.1 Ref.5

Post-translational modification

Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.

Sequence similarities

Belongs to the WD repeat SEC31 family.

Contains 8 WD repeats.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NQW1-1)

Also known as: SEC31B-F;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NQW1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     165-165: Missing.
     349-349: I → V
     350-1179: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 3 (identifier: Q9NQW1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     69-85: FHKLVWGSFGSGLLESS → ATSWLQGPAILKSSLGI
     86-1179: Missing.
Isoform 4 (identifier: Q9NQW1-4)

Also known as: SEC31B-t;

The sequence of this isoform differs from the canonical sequence as follows:
     470-482: KVTLEQDSRMKFL → KPARDSSGARR
     483-1179: Missing.
Isoform 5 (identifier: Q9NQW1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     165-165: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11791179Protein transport protein Sec31B
PRO_0000295153

Regions

Repeat17 – 5640WD 1
Repeat67 – 11044WD 2
Repeat119 – 15941WD 3
Repeat166 – 20641WD 4
Repeat209 – 25446WD 5
Repeat258 – 29841WD 6
Repeat301 – 34141WD 7
Repeat391 – 42232WD 8; interaction with SEC13 By similarity
Compositional bias821 – 1073253Pro-rich

Natural variations

Alternative sequence69 – 8517FHKLV…LLESS → ATSWLQGPAILKSSLGI in isoform 3.
VSP_026757
Alternative sequence86 – 11791094Missing in isoform 3.
VSP_026758
Alternative sequence1651Missing in isoform 2 and isoform 5.
VSP_026759
Alternative sequence3491I → V in isoform 2.
VSP_026760
Alternative sequence350 – 1179830Missing in isoform 2.
VSP_026761
Alternative sequence470 – 48213KVTLE…RMKFL → KPARDSSGARR in isoform 4.
VSP_026762
Alternative sequence483 – 1179697Missing in isoform 4.
VSP_026763
Natural variant891V → A. Ref.4
Corresponds to variant rs3763695 [ dbSNP | Ensembl ].
VAR_033228
Natural variant1001Y → C.
Corresponds to variant rs7074707 [ dbSNP | Ensembl ].
VAR_033229
Natural variant1291L → F. Ref.4
Corresponds to variant rs3793706 [ dbSNP | Ensembl ].
VAR_033230
Natural variant3321S → A. Ref.4
Corresponds to variant rs2295774 [ dbSNP | Ensembl ].
VAR_033231
Natural variant3721P → S.
Corresponds to variant rs2295772 [ dbSNP | Ensembl ].
VAR_033232
Natural variant4331R → Q.
Corresponds to variant rs2295771 [ dbSNP | Ensembl ].
VAR_053415
Natural variant4781R → T.
Corresponds to variant rs11819496 [ dbSNP | Ensembl ].
VAR_053416
Natural variant5271S → R.
Corresponds to variant rs17113157 [ dbSNP | Ensembl ].
VAR_033233
Natural variant11691A → S.
Corresponds to variant rs2298075 [ dbSNP | Ensembl ].
VAR_033234

Experimental info

Sequence conflict8051A → V in CAB45735. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SEC31B-F) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 88C591B060643A5D

FASTA1,179128,697
        10         20         30         40         50         60 
MKLKELERPA VQAWSPASQY PLYLATGTSA QQLDSSFSTN GTLEIFEVDF RDPSLDLKHR 

        70         80         90        100        110        120 
GVLSALSRFH KLVWGSFGSG LLESSGVIVG GGDNGMLILY NVTHILSSGK EPVIAQKQKH 

       130        140        150        160        170        180 
TGAVRALDLN PFQGNLLASG ASDSEIFIWD LNNLNVPMTL GSKSQQPPED IKALSWNRQA 

       190        200        210        220        230        240 
QHILSSAHPS GKAVVWDLRK NEPIIKVSDH SNRMHCSGLA WHPDIATQLV LCSEDDRLPV 

       250        260        270        280        290        300 
IQLWDLRFAS SPLKVLESHS RGILSVSWSQ ADAELLLTSA KDSQILCRNL GSSEVVYKLP 

       310        320        330        340        350        360 
TQSSWCFDVQ WCPRDPSVFS AASFNGWISL YSVMGRSWEV QHMRQADKIS SSFSKGQPLP 

       370        380        390        400        410        420 
PLQVPEQVAQ APLIPPLKKP PKWIRRPTGV SFAFGGKLVT FGLPSTPAHL VPQPCPRLVF 

       430        440        450        460        470        480 
ISQVTTESEF LMRSAELQEA LGSGNLLNYC QNKSQQALLQ SEKMLWQFLK VTLEQDSRMK 

       490        500        510        520        530        540 
FLKLLGYSKD ELQKKVATWL KSDVGLGESP QPKGNDLNSD RQQAFCSQAS KHTTKEASAS 

       550        560        570        580        590        600 
SAFFDELVPQ NMTPWEIPIT KDIDGLLSQA LLLGELGPAV ELCLKEERFA DAIILAQAGG 

       610        620        630        640        650        660 
TDLLKQTQER YLAKKKTKIS SLLACVVQKN WKDVVCTCSL KNWREALALL LTYSGTEKFP 

       670        680        690        700        710        720 
ELCDMLGTRM EQEGSRALTS EARLCYVCSG SVERLVECWA KCHQALSPMA LQDLMEKVMV 

       730        740        750        760        770        780 
LNRSLEQLRG PHGVSPGPAT TYRVTQYANL LAAQGSLATA MSFLPRDCAQ PPVQQLRDRL 

       790        800        810        820        830        840 
FHAQGSAVLG QQSPPFPFPR IVVGATLHSK ETSSYRLGSQ PSHQVPTPSP RPRVFTPQSS 

       850        860        870        880        890        900 
PAMPLAPSHP SPYQGPRTQN ISDYRAPGPQ AIQPLPLSPG VRPASSQPQL LGGQRVQVPN 

       910        920        930        940        950        960 
PVGFPGTWPL PGSPLPMACP GIMRPGSTSL PETPRLFPLL PLRPLGPGRM VSHTPAPPAS 

       970        980        990       1000       1010       1020 
FPVPYLPGDP GAPCSSVLPT TGILTPHPGP QDSWKEAPAP RGNLQRNKLP ETFMPPAPIT 

      1030       1040       1050       1060       1070       1080 
APVMSLTPEL QGILPSQPPV SSVSHAPPGV PGELSLQLQH LPPEKMERKE LPPEHQSLKS 

      1090       1100       1110       1120       1130       1140 
SFEALLQRCS LSATDLKTKR KLEEAAQRLE YLYEKLCEGT LSPHVVAGLH EVARCVDAGS 

      1150       1160       1170 
FEQGLAVHAQ VAGCSSFSEV SSFMPILKAV LIIAHKLLV 

« Hide

Isoform 2 [UniParc].

Checksum: BD8A60259743B860
Show »

FASTA34838,450
Isoform 3 [UniParc].

Checksum: 1CA9C2A4C9F56411
Show »

FASTA859,294
Isoform 4 (SEC31B-t) [UniParc].

Checksum: A91F37DAA6824103
Show »

FASTA48052,984
Isoform 5 [UniParc].

Checksum: 102AA849D41A1D65
Show »

FASTA1,178128,569

References

« Hide 'large scale' references
[1]"Human Sec31B: a family of new mammalian orthologues of yeast Sec31p that associate with the COPII coat."
Stankewich M.C., Stabach P.R., Morrow J.S.
J. Cell Sci. 119:958-969(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 4), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH SEC13 AND SEC31A.
Tissue: Testis.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 265-1179 (ISOFORM 1).
Tissue: Testis.
[3]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5), VARIANTS ALA-89; PHE-129 AND ALA-332.
Tissue: Testis.
[5]"Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport."
Tang B.L., Zhang T., Low D.Y.H., Wong E.T., Horstmann H., Hong W.
J. Biol. Chem. 275:13597-13604(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Ubiquitin-dependent regulation of COPII coat size and function."
Jin L., Pahuja K.B., Wickliffe K.E., Gorur A., Baumgartel C., Schekman R., Rape M.
Nature 482:495-500(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF274863 mRNA. Translation: AAF78243.1.
AL080141 mRNA. Translation: CAB45735.1.
BX640914 mRNA. Translation: CAE45955.1.
AL133352 Genomic DNA. Translation: CAH73561.1.
BC034946 mRNA. Translation: AAH34946.1.
BC044569 mRNA. No translation available.
BC144313 mRNA. Translation: AAI44314.1.
CCDSCCDS7495.1. [Q9NQW1-1]
PIRT12526.
RefSeqNP_056305.1. NM_015490.3. [Q9NQW1-1]
UniGeneHs.18889.

3D structure databases

ProteinModelPortalQ9NQW1.
SMRQ9NQW1. Positions 5-699.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117447. 3 interactions.
IntActQ9NQW1. 4 interactions.
STRING9606.ENSP00000359370.

PTM databases

PhosphoSiteQ9NQW1.

Polymorphism databases

DMDM74762753.

Proteomic databases

PaxDbQ9NQW1.
PRIDEQ9NQW1.

Protocols and materials databases

DNASU25956.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370345; ENSP00000359370; ENSG00000075826. [Q9NQW1-1]
ENST00000451524; ENSP00000391178; ENSG00000075826. [Q9NQW1-4]
ENST00000462434; ENSP00000474124; ENSG00000075826. [Q9NQW1-2]
GeneID25956.
KEGGhsa:25956.
UCSCuc001krc.1. human. [Q9NQW1-1]
uc010qpo.1. human. [Q9NQW1-5]

Organism-specific databases

CTD25956.
GeneCardsGC10M102237.
HGNCHGNC:23197. SEC31B.
HPAHPA035882.
HPA040819.
MIM610258. gene.
neXtProtNX_Q9NQW1.
PharmGKBPA162402792.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG248389.
HOGENOMHOG000230582.
HOVERGENHBG055626.
InParanoidQ9NQW1.
KOK14005.
OMACSLKNWR.
OrthoDBEOG7B31M1.
PhylomeDBQ9NQW1.
TreeFamTF313842.

Enzyme and pathway databases

SignaLinkQ9NQW1.

Gene expression databases

ArrayExpressQ9NQW1.
BgeeQ9NQW1.
CleanExHS_SEC31B.
GenevestigatorQ9NQW1.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSEC31B. human.
GenomeRNAi25956.
NextBio47564.
PROQ9NQW1.
SOURCESearch...

Entry information

Entry nameSC31B_HUMAN
AccessionPrimary (citable) accession number: Q9NQW1
Secondary accession number(s): B7ZM75 expand/collapse secondary AC list , Q6MZS3, Q86UF0, Q9Y4Q8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM