Q9NQS5 (GPR84_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 84

Alternative name(s):
Inflammation-related G-protein coupled receptor EX33

Gene names

Name:	GPR84
Synonyms:	EX33

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system. Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes. Ref.1 Ref.2 Ref.7
Induction	By bacterial lipopolysaccharides (LPS) in the monocytic leukaemia cell line THP-1. Ref.7
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	G-protein coupled receptor 84		PRO_0000069589
Regions
	Topological domain	1 – 26	26	Extracellular Potential
	Transmembrane	27 – 47	21	Helical; Name=1; Potential
	Topological domain	48 – 57	10	Cytoplasmic Potential
	Transmembrane	58 – 78	21	Helical; Name=2; Potential
	Topological domain	79 – 94	16	Extracellular Potential
	Transmembrane	95 – 115	21	Helical; Name=3; Potential
	Topological domain	116 – 144	29	Cytoplasmic Potential
	Transmembrane	145 – 165	21	Helical; Name=4; Potential
	Topological domain	166 – 180	15	Extracellular Potential
	Transmembrane	181 – 201	21	Helical; Name=5; Potential
	Topological domain	202 – 320	119	Cytoplasmic Potential
	Transmembrane	321 – 341	21	Helical; Name=6; Potential
	Topological domain	342 – 352	11	Extracellular Potential
	Transmembrane	353 – 373	21	Helical; Name=7; Potential
	Topological domain	374 – 396	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	221	1	Phosphoserine By similarity
	Modified residue	224	1	Phosphoserine By similarity
	Modified residue	391	1	Phosphoserine By similarity
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	37	1	G → D. Corresponds to variant rs11170883 [ dbSNP | Ensembl ].		VAR_049397

Sequences

	Sequence		Length	Mass (Da)
	Q9NQS5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BEEA467DFEB7520B	FASTA	396	43,705
	10 20 30 40 50 60 MWNSSDANFS CYHESVLGYR YVAVSWGVVV AVTGTVGNVL TLLALAIQPK LRTRFNLLIA 70 80 90 100 110 120 NLTLADLLYC TLLQPFSVDT YLHLHWRTGA TFCRVFGLLL FASNSVSILT LCLIALGRYL 130 140 150 160 170 180 LIAHPKLFPQ VFSAKGIVLA LVSTWVVGVA SFAPLWPIYI LVPVVCTCSF DRIRGRPYTT 190 200 210 220 230 240 ILMGIYFVLG LSSVGIFYCL IHRQVKRAAQ ALDQYKLRQA SIHSNHVART DEAMPGRFQE 250 260 270 280 290 300 LDSRLASGGP SEGISSEPVS AATTQTLEGD SSEVGDQINS KRAKQMAEKS PPEASAKAQP 310 320 330 340 350 360 IKGARRAPDS SSEFGKVTRM CFAVFLCFAL SYIPFLLLNI LDARVQAPRV VHMLAANLTW 370 380 390 LNGCINPVLY AAMNRQFRQA YGSILKRGPR SFHRLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression analysis of a novel G-protein-coupled receptor selectively expressed on granulocytes." Yousefi S., Cooper P.R., Potter S.L., Mueck B., Jarai G. J. Leukoc. Biol. 69:1045-1052(2001) [PubMed: 11404393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors." Wittenberger T., Schaller H.C., Hellebrand S. J. Mol. Biol. 307:799-813(2001) [PubMed: 11273702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"Isolation of cDNA coding for Homo sapiens G protein-coupled receptor 84 (GPR84)." Kaighin V.A., Martin A.L., Aronstam R.S. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[4]	"Identification of G protein-coupled receptor genes from the human genome sequence." Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S. FEBS Lett. 520:97-101(2002) [PubMed: 12044878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84." Wang J., Wu X., Simonavicius N., Tian H., Ling L. J. Biol. Chem. 281:34457-34464(2006) [PubMed: 16966319] [Abstract] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF282693 mRNA. Translation: AAF91467.1. AF237762 mRNA. Translation: AAK01857.1. FJ348261 mRNA. Translation: ACI96305.1. AB083586 Genomic DNA. Translation: BAB89299.1. CH471054 Genomic DNA. Translation: EAW96776.1. BC020614 mRNA. Translation: AAH20614.1.
IPI	IPI00006903.
RefSeq	NP_065103.1. NM_020370.2.
UniGene	Hs.306199.
3D structure databases
ProteinModelPortal	Q9NQS5.
SMR	Q9NQS5. Positions 105-136, 352-388.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NQS5.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	66774035.
Proteomic databases
PRIDE	Q9NQS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000267015; ENSP00000267015; ENSG00000139572.
GeneID	53831.
KEGG	hsa:53831.
UCSC	uc001sfu.1. human.
Organism-specific databases
CTD	53831.
GeneCards	GC12M054756.
HGNC	HGNC:4535. GPR84.
HPA	HPA027603.
MIM	606383. gene.
neXtProt	NX_Q9NQS5.
PharmGKB	PA28928.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12629.
GeneTree	ENSGT00600000084537.
HOGENOM	HBG446643.
HOVERGEN	HBG051820.
InParanoid	Q9NQS5.
OMA	QPFSVDT.
OrthoDB	EOG4ZKJMN.
PhylomeDB	Q9NQS5.
Gene expression databases
ArrayExpress	Q9NQS5.
Bgee	Q9NQS5.
CleanEx	HS_GPR84.
Genevestigator	Q9NQS5.
GermOnline	ENSG00000139572. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
KO	K08421.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	56158.
SOURCE	Search...

Entry information

Entry name

GPR84_HUMAN

Accession

Primary (citable) accession number: Q9NQS5
Secondary accession number(s): B6V9G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.