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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4

Gene

Ndufaf4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in cell proliferation and survival of hormone-dependent tumor cells. Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) (By similarity).By similarity1 Publication

GO - Biological processi

  • mitochondrial respiratory chain complex I assembly Source: InterPro
  • negative regulation of apoptotic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-RNO-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
Alternative name(s):
Hormone-regulated proliferation-associated protein of 20 kDa homolog
Protein HRPAP20
Gene namesi
Name:Ndufaf4
Synonyms:Hrpap20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi735162. Ndufaf4.

Subcellular locationi

  • Mitochondrion By similarity
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 174173NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4PRO_0000342666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei35 – 351PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serine. Prolactin stimulate serine phosphorylation.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9NQR8.
PRIDEiQ9NQR8.

Expressioni

Inductioni

Expression is low in quiescent cells and is induced in exponentially proliferating cultures. Expression is also induced when prolactin is added to stationary cells. Induced by dietary differentiating agents such as butyrate, vitamin D3, and retinoic acid.1 Publication

Gene expression databases

GenevisibleiQ9NQR8. RN.

Interactioni

Subunit structurei

Binds calmodulin. Interacts with NDUFAF3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010072.

Family & Domainsi

Sequence similaritiesi

Belongs to the NDUFAF4 family.Curated

Phylogenomic databases

eggNOGiKOG4481. Eukaryota.
ENOG4111W8K. LUCA.
GeneTreeiENSGT00390000001627.
HOGENOMiHOG000231584.
InParanoidiQ9NQR8.
KOiK18161.
OMAiKFINDHQ.
OrthoDBiEOG7FBRKN.
PhylomeDBiQ9NQR8.
TreeFamiTF323532.

Family and domain databases

InterProiIPR009622. NDUFAF4.
[Graphical view]
PANTHERiPTHR13338. PTHR13338. 1 hit.
PfamiPF06784. UPF0240. 1 hit.
[Graphical view]
ProDomiPD339506. UPF0240. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NQR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGARMTRAFR NFNVEKRAEQ EISKRKPSMA PKHPSTRSLL QEHLSQYPEI
60 70 80 90 100
EEEVSRKDNK LLSLLRDVYV NSKDPVPSLP VKAIEPQQQP KEFRLPIGDQ
110 120 130 140 150
FDKNIKDIPK GKITVVEALT LLNNHKLSPE TWTAEKIAQE YHLELEDVNS
160 170
LLKYFVTFEV KIVPPEDRKA IQSK
Length:174
Mass (Da):20,158
Last modified:October 1, 2000 - v1
Checksum:iAC93F13A4EBABB5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283900 mRNA. Translation: AAF90196.1.
RefSeqiNP_942078.1. NM_198783.1.
UniGeneiRn.106694.

Genome annotation databases

EnsembliENSRNOT00000010073; ENSRNOP00000010072; ENSRNOG00000007506.
GeneIDi362495.
KEGGirno:362495.
UCSCiRGD:735162. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283900 mRNA. Translation: AAF90196.1.
RefSeqiNP_942078.1. NM_198783.1.
UniGeneiRn.106694.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010072.

Proteomic databases

PaxDbiQ9NQR8.
PRIDEiQ9NQR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010073; ENSRNOP00000010072; ENSRNOG00000007506.
GeneIDi362495.
KEGGirno:362495.
UCSCiRGD:735162. rat.

Organism-specific databases

CTDi29078.
RGDi735162. Ndufaf4.

Phylogenomic databases

eggNOGiKOG4481. Eukaryota.
ENOG4111W8K. LUCA.
GeneTreeiENSGT00390000001627.
HOGENOMiHOG000231584.
InParanoidiQ9NQR8.
KOiK18161.
OMAiKFINDHQ.
OrthoDBiEOG7FBRKN.
PhylomeDBiQ9NQR8.
TreeFamiTF323532.

Enzyme and pathway databases

ReactomeiR-RNO-6799198. Complex I biogenesis.

Miscellaneous databases

PROiQ9NQR8.

Gene expression databases

GenevisibleiQ9NQR8. RN.

Family and domain databases

InterProiIPR009622. NDUFAF4.
[Graphical view]
PANTHERiPTHR13338. PTHR13338. 1 hit.
PfamiPF06784. UPF0240. 1 hit.
[Graphical view]
ProDomiPD339506. UPF0240. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "Identification of HRPAP20: a novel phosphoprotein that enhances growth and survival in hormone-responsive tumor cells."
    Karp C.M., Pan H., Zhang M., Buckley D.J., Schuler L.A., Buckley A.R.
    Cancer Res. 64:1016-1025(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, PHOSPHORYLATION.

Entry informationi

Entry nameiNDUF4_RAT
AccessioniPrimary (citable) accession number: Q9NQR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.