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Protein

Omega-amidase NIT2

Gene

NIT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.2 Publications

Catalytic activityi

A monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3.

Kineticsi

In solution, alpha-ketoglutaramate is in equilibrium with a cyclic form (2-hydroxy-5-oxoproline), and at pH 8.0 or above, the rate of ring opening is no longer limiting for the omega-amidase reaction.

  1. KM=0.009 mM for alpha-ketoglutaramate (open-chain form)1 Publication
  1. Vmax=5.9 µmol/min/mg enzyme with alpha-ketoglutaramate as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431Proton acceptorPROSITE-ProRule annotation
Active sitei112 – 1121Proton donorPROSITE-ProRule annotation
Active sitei153 – 1531NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  • omega-amidase activity Source: BHF-UCL

GO - Biological processi

  • asparagine metabolic process Source: BHF-UCL
  • glutamine metabolic process Source: BHF-UCL
  • oxaloacetate metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-amidase NIT2 (EC:3.5.1.3)
Alternative name(s):
Nitrilase homolog 2
Gene namesi
Name:NIT2
ORF Names:CUA002
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29878. NIT2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: HPA
  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134882857.

Polymorphism and mutation databases

BioMutaiNIT2.
DMDMi74725271.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Omega-amidase NIT2PRO_0000320252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261PhosphoserineCombined sources
Modified residuei68 – 681N6-acetyllysine; alternateBy similarity
Modified residuei68 – 681N6-succinyllysine; alternateBy similarity
Modified residuei123 – 1231N6-succinyllysineBy similarity
Modified residuei130 – 1301N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NQR4.
PaxDbiQ9NQR4.
PeptideAtlasiQ9NQR4.
PRIDEiQ9NQR4.

2D gel databases

REPRODUCTION-2DPAGEIPI00549467.
UCD-2DPAGEQ9NQR4.

PTM databases

iPTMnetiQ9NQR4.
PhosphoSiteiQ9NQR4.

Expressioni

Tissue specificityi

Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.2 Publications

Gene expression databases

BgeeiENSG00000114021.
CleanExiHS_NIT2.
ExpressionAtlasiQ9NQR4. baseline and differential.
GenevisibleiQ9NQR4. HS.

Organism-specific databases

HPAiHPA036999.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi121278. 24 interactions.
IntActiQ9NQR4. 2 interactions.
STRINGi9606.ENSP00000377696.

Structurei

3D structure databases

ProteinModelPortaliQ9NQR4.
SMRiQ9NQR4. Positions 1-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 270267CN hydrolasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0806. Eukaryota.
COG0388. LUCA.
GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
HOVERGENiHBG105126.
InParanoidiQ9NQR4.
KOiK13566.
OMAiNPWGEVI.
OrthoDBiEOG091G0IKZ.
PhylomeDBiQ9NQR4.
TreeFamiTF300747.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NQR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG
60 70 80 90 100
AKYFPEYAEK IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF
110 120 130 140 150
GPDGTLLAKY RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL
160 170 180 190 200
GICYDMRFAE LAQIYAQRGC QLLVYPGAFN LTTGPAHWEL LQRSRAVDNQ
210 220 230 240 250
VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA IVYSDIDLKK
260 270
LAEIRQQIPV FRQKRSDLYA VEMKKP
Length:276
Mass (Da):30,608
Last modified:October 1, 2000 - v1
Checksum:i32FA797601A419C6
GO

Mass spectrometryi

Molecular mass is 30585 Da from positions 1 - 276. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti231 – 2311V → A.1 Publication
Corresponds to variant rs17851799 [ dbSNP | Ensembl ].
VAR_039180

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284574 mRNA. Translation: AAF87103.1.
AF260334 mRNA. Translation: AAG44665.1.
AK313704 mRNA. Translation: BAG36450.1.
CH471052 Genomic DNA. Translation: EAW79824.1.
CH471052 Genomic DNA. Translation: EAW79825.1.
BC020620 mRNA. Translation: AAH20620.1.
BC107890 mRNA. Translation: AAI07891.1.
CCDSiCCDS33806.1.
RefSeqiNP_064587.1. NM_020202.4.
UniGeneiHs.439152.

Genome annotation databases

EnsembliENST00000394140; ENSP00000377696; ENSG00000114021.
GeneIDi56954.
KEGGihsa:56954.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284574 mRNA. Translation: AAF87103.1.
AF260334 mRNA. Translation: AAG44665.1.
AK313704 mRNA. Translation: BAG36450.1.
CH471052 Genomic DNA. Translation: EAW79824.1.
CH471052 Genomic DNA. Translation: EAW79825.1.
BC020620 mRNA. Translation: AAH20620.1.
BC107890 mRNA. Translation: AAI07891.1.
CCDSiCCDS33806.1.
RefSeqiNP_064587.1. NM_020202.4.
UniGeneiHs.439152.

3D structure databases

ProteinModelPortaliQ9NQR4.
SMRiQ9NQR4. Positions 1-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121278. 24 interactions.
IntActiQ9NQR4. 2 interactions.
STRINGi9606.ENSP00000377696.

PTM databases

iPTMnetiQ9NQR4.
PhosphoSiteiQ9NQR4.

Polymorphism and mutation databases

BioMutaiNIT2.
DMDMi74725271.

2D gel databases

REPRODUCTION-2DPAGEIPI00549467.
UCD-2DPAGEQ9NQR4.

Proteomic databases

EPDiQ9NQR4.
PaxDbiQ9NQR4.
PeptideAtlasiQ9NQR4.
PRIDEiQ9NQR4.

Protocols and materials databases

DNASUi56954.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394140; ENSP00000377696; ENSG00000114021.
GeneIDi56954.
KEGGihsa:56954.

Organism-specific databases

CTDi56954.
GeneCardsiNIT2.
HGNCiHGNC:29878. NIT2.
HPAiHPA036999.
neXtProtiNX_Q9NQR4.
PharmGKBiPA134882857.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0806. Eukaryota.
COG0388. LUCA.
GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
HOVERGENiHBG105126.
InParanoidiQ9NQR4.
KOiK13566.
OMAiNPWGEVI.
OrthoDBiEOG091G0IKZ.
PhylomeDBiQ9NQR4.
TreeFamiTF300747.

Miscellaneous databases

GenomeRNAii56954.
PROiQ9NQR4.

Gene expression databases

BgeeiENSG00000114021.
CleanExiHS_NIT2.
ExpressionAtlasiQ9NQR4. baseline and differential.
GenevisibleiQ9NQR4. HS.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIT2_HUMAN
AccessioniPrimary (citable) accession number: Q9NQR4
Secondary accession number(s): B2R9A3, D3DN47, Q8WUF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.