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Protein

Probable ATP-dependent RNA helicase DDX4

Gene

DDX4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).By similarity2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi332 – 339ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Helicase, Hydrolase
Biological processDifferentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.13 2681
ReactomeiR-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX4 (EC:3.6.4.13By similarity)
Alternative name(s):
DEAD box protein 4
Vasa homolog
Gene namesi
Name:DDX4
Synonyms:VASA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000152670.18
HGNCiHGNC:18700 DDX4
MIMi605281 gene
neXtProtiNX_Q9NQI0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi54514
OpenTargetsiENSG00000152670
PharmGKBiPA38646

Polymorphism and mutation databases

BioMutaiDDX4
DMDMi20138033

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549771 – 724Probable ATP-dependent RNA helicase DDX4Add BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei722PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NQI0
MaxQBiQ9NQI0
PaxDbiQ9NQI0
PeptideAtlasiQ9NQI0
PRIDEiQ9NQI0
ProteomicsDBi82159
82160 [Q9NQI0-2]

2D gel databases

REPRODUCTION-2DPAGEiIPI00456933

PTM databases

iPTMnetiQ9NQI0
PhosphoSitePlusiQ9NQI0

Expressioni

Tissue specificityi

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.1 Publication

Gene expression databases

BgeeiENSG00000152670
CleanExiHS_DDX4
ExpressionAtlasiQ9NQI0 baseline and differential
GenevisibleiQ9NQI0 HS

Organism-specific databases

HPAiCAB026170
HPA037763
HPA037764

Interactioni

Subunit structurei

Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Interacts with ARNTL/BMAL1 and CLOCK. Interacts with TEX19 (By similarity).By similarity

Protein-protein interaction databases

BioGridi120009, 9 interactors
IntActiQ9NQI0, 3 interactors
MINTiQ9NQI0
STRINGi9606.ENSP00000424838

Structurei

3D structure databases

DisProtiDP01065
DP01066
ProteinModelPortaliQ9NQI0
SMRiQ9NQI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini319 – 502Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST184
Domaini530 – 675Helicase C-terminalPROSITE-ProRule annotationAdd BLAST146

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi288 – 316Q motifAdd BLAST29
Motifi446 – 449DEAD boxBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi58 – 234Gly-richAdd BLAST177

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0335 Eukaryota
ENOG410XNTI LUCA
GeneTreeiENSGT00900000140933
HOGENOMiHOG000268804
HOVERGENiHBG015893
InParanoidiQ9NQI0
KOiK13982
OMAiFRGCRGG
OrthoDBiEOG091G04U3
PhylomeDBiQ9NQI0
TreeFamiTF300364

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NQI0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDEDWEAEI NPHMSSYVPI FEKDRYSGEN GDNFNRTPAS SSEMDDGPSR
60 70 80 90 100
RDHFMKSGFA SGRNFGNRDA GECNKRDNTS TMGGFGVGKS FGNRGFSNSR
110 120 130 140 150
FEDGDSSGFW RESSNDCEDN PTRNRGFSKR GGYRDGNNSE ASGPYRRGGR
160 170 180 190 200
GSFRGCRGGF GLGSPNNDLD PDECMQRTGG LFGSRRPVLS GTGNGDTSQS
210 220 230 240 250
RSGSGSERGG YKGLNEEVIT GSGKNSWKSE AEGGESSDTQ GPKVTYIPPP
260 270 280 290 300
PPEDEDSIFA HYQTGINFDK YDTILVEVSG HDAPPAILTF EEANLCQTLN
310 320 330 340 350
NNIAKAGYTK LTPVQKYSIP IILAGRDLMA CAQTGSGKTA AFLLPILAHM
360 370 380 390 400
MHDGITASRF KELQEPECII VAPTRELVNQ IYLEARKFSF GTCVRAVVIY
410 420 430 440 450
GGTQLGHSIR QIVQGCNILC ATPGRLMDII GKEKIGLKQI KYLVLDEADR
460 470 480 490 500
MLDMGFGPEM KKLISCPGMP SKEQRQTLMF SATFPEEIQR LAAEFLKSNY
510 520 530 540 550
LFVAVGQVGG ACRDVQQTVL QVGQFSKREK LVEILRNIGD ERTMVFVETK
560 570 580 590 600
KKADFIATFL CQEKISTTSI HGDREQRERE QALGDFRFGK CPVLVATSVA
610 620 630 640 650
ARGLDIENVQ HVINFDLPST IDEYVHRIGR TGRCGNTGRA ISFFDLESDN
660 670 680 690 700
HLAQPLVKVL TDAQQDVPAW LEEIAFSTYI PGFSGSTRGN VFASVDTRKG
710 720
KSTLNTAGFS SSQAPNPVDD ESWD
Length:724
Mass (Da):79,308
Last modified:January 23, 2002 - v2
Checksum:i6D313DD98B177067
GO
Isoform 2 (identifier: Q9NQI0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-166: GYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPN → D

Note: No experimental confirmation available.
Show »
Length:690
Mass (Da):75,821
Checksum:i8D37259B5DED0EBD
GO
Isoform 3 (identifier: Q9NQI0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: MGDEDWEAEI...EDGDSSGFWR → MGSRNLFLTNSP
     132-166: GYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPN → D
     209-225: GGYKGLNEEVITGSGKN → D

Note: No experimental confirmation available.
Show »
Length:575
Mass (Da):63,211
Checksum:i04F943C83BD71828
GO
Isoform 4 (identifier: Q9NQI0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-131: Missing.

Note: No experimental confirmation available. Gene prediction based on cDNA data.
Show »
Length:704
Mass (Da):77,056
Checksum:iA1A44F186461C9D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240Q → R in BAG52716 (PubMed:14702039).Curated1
Sequence conflicti380Q → K in AAH47455 (PubMed:15489334).Curated1
Sequence conflicti556I → T in AAF86585 (PubMed:10920202).Curated1
Sequence conflicti713Q → R in AAF86585 (PubMed:10920202).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019574148G → D. Corresponds to variant dbSNP:rs2306259Ensembl.1
Natural variantiVAR_052159287I → V. Corresponds to variant dbSNP:rs2305123Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0461321 – 111MGDED…SGFWR → MGSRNLFLTNSP in isoform 3. 1 PublicationAdd BLAST111
Alternative sequenceiVSP_047177112 – 131Missing in isoform 4. CuratedAdd BLAST20
Alternative sequenceiVSP_011197132 – 166GYRDG…LGSPN → D in isoform 2 and isoform 3. 2 PublicationsAdd BLAST35
Alternative sequenceiVSP_046133209 – 225GGYKG…GSGKN → D in isoform 3. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY004154 mRNA Translation: AAF86585.1
AF262962 mRNA Translation: AAF72705.1
AK093439 mRNA Translation: BAG52716.1
AK292417 mRNA Translation: BAF85106.1
AC016632 Genomic DNA No translation available.
CH471123 Genomic DNA Translation: EAW54929.1
BC047455 mRNA Translation: AAH47455.1
BC088362 mRNA Translation: AAH88362.1
AL137462 mRNA Translation: CAB70750.1
CCDSiCCDS3969.1 [Q9NQI0-1]
CCDS47208.1 [Q9NQI0-2]
CCDS54854.1 [Q9NQI0-4]
CCDS54855.1 [Q9NQI0-3]
PIRiT46407
RefSeqiNP_001136021.1, NM_001142549.1 [Q9NQI0-2]
NP_001160005.1, NM_001166533.1 [Q9NQI0-4]
NP_001160006.1, NM_001166534.1 [Q9NQI0-3]
NP_077726.1, NM_024415.2 [Q9NQI0-1]
UniGeneiHs.223581

Genome annotation databases

EnsembliENST00000353507; ENSP00000334167; ENSG00000152670 [Q9NQI0-2]
ENST00000354991; ENSP00000347087; ENSG00000152670 [Q9NQI0-2]
ENST00000505374; ENSP00000424838; ENSG00000152670 [Q9NQI0-1]
ENST00000511853; ENSP00000423123; ENSG00000152670 [Q9NQI0-3]
ENST00000514278; ENSP00000425359; ENSG00000152670 [Q9NQI0-4]
GeneIDi54514
KEGGihsa:54514
UCSCiuc003jqg.5 human [Q9NQI0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDDX4_HUMAN
AccessioniPrimary (citable) accession number: Q9NQI0
Secondary accession number(s): A8K8Q2
, B3KSF4, D6RDK4, E9PCD8, Q5M7Z3, Q86VX0, Q9NT92, Q9NYB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: June 20, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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