Q9NQC1 (JADE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Jade-2

Alternative name(s):
PHD finger protein 15

Gene names

Name:	PHF15
Synonyms:	JADE2, KIAA0239

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Ref.6
Subunit structure	Component of the HBO1 complex composed at least of ING4 or ING5, MYST2/HBO1, MEAF6, and one of PHF15, PHF16 and PHF17. Ref.6
Sequence similarities	Belongs to the JADE family. Contains 1 PHD-type zinc finger.
Sequence caution	The sequence BAA13245.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB84949.1 differs from that shown. Reason: Frameshift at position 11. The sequence CAB94935.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H3 acetylation Inferred from direct assay Ref.6. Source: UniProtKB histone H4-K12 acetylation Inferred from direct assay Ref.6. Source: UniProtKB histone H4-K5 acetylation Inferred from direct assay Ref.6. Source: UniProtKB histone H4-K8 acetylation Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	histone acetyltransferase complex Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQC1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQC1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     518-518: Missing.

Isoform 3 (identifier: Q9NQC1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     518-790: GLSTSFPIDG...EVVRMGVLAS → ERSGRRAKGK...PDSVLGQLGE

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 790	790	Protein Jade-2		PRO_0000059308
Regions
	Zinc finger	199 – 249	51	PHD-type
	Compositional bias	601 – 732	132	Pro-rich
Amino acid modifications
	Modified residue	32	1	N6-acetyllysine Ref.8
	Modified residue	38	1	N6-acetyllysine Ref.8
	Modified residue	298	1	N6-acetyllysine Ref.8
Natural variations
	Alternative sequence	518 – 790	273	GLSTS…GVLAS → ERSGRRAKGKKSDSKRKGCE GSKGSTEKKEKVKAGPDSVL GQLGE in isoform 3.		VSP_021050
	Alternative sequence	518	1	Missing in isoform 2.		VSP_021051
	Natural variant	581	1	R → G. Corresponds to variant rs34200923 [ dbSNP | Ensembl ].		VAR_053778
Experimental info
	Sequence conflict	331	1	T → I in BAA13245. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 22C1B1A0360BC1AE	FASTA	790	87,466
	10 20 30 40 50 60 MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA 70 80 90 100 110 120 MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRILPPLE GPPAQASPSS 130 140 150 160 170 180 TMLGEGSQPD WPGGSRYDLD EIDAYWLELI NSELKEMERP ELDELTLERV LEELETLCHQ 190 200 210 220 230 240 NMARAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS 250 260 270 280 290 300 WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS 310 320 330 340 350 360 HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA DNDEVKFKSF 370 380 390 400 410 420 CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL EEDFYELVEP AEVAERLDLA 430 440 450 460 470 480 EALVDFIYQY WKLKRKANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR 490 500 510 520 530 540 NLCYMVTRRE RTKHAICKLQ EQIFHLQMKL IEQDLCRGLS TSFPIDGTFF NSWLAQSVQI 550 560 570 580 590 600 TAENMAMSEW PLNNGHREDP APGLLSEELL QDEETLLSFM RDPSLRPGDP ARKARGRTRL 610 620 630 640 650 660 PAKKKPPPPP PQDGPGSRTT PDKAPKKTWG QDAGSGKGGQ GPPTRKPPRR TSSHLPSSPA 670 680 690 700 710 720 AGDCPILATP ESPPPLAPET PDEAASVAAD SDVQVPGPAA SPKPLGRLRP PRESKVTRRL 730 740 750 760 770 780 PGARPDAGMG PPSAVAERPK VSLHFDTETD GYFSDGEMSD SDVEAEDGGV QRGPREAGAE 790 EVVRMGVLAS
	Isoform 2 [UniParc]. Checksum: EFCA863CE55B6297	FASTA	789	87,409
	10 20 30 40 50 60 MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA 70 80 90 100 110 120 MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRILPPLE GPPAQASPSS 130 140 150 160 170 180 TMLGEGSQPD WPGGSRYDLD EIDAYWLELI NSELKEMERP ELDELTLERV LEELETLCHQ 190 200 210 220 230 240 NMARAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS 250 260 270 280 290 300 WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS 310 320 330 340 350 360 HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA DNDEVKFKSF 370 380 390 400 410 420 CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL EEDFYELVEP AEVAERLDLA 430 440 450 460 470 480 EALVDFIYQY WKLKRKANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR 490 500 510 520 530 540 NLCYMVTRRE RTKHAICKLQ EQIFHLQMKL IEQDLCRLST SFPIDGTFFN SWLAQSVQIT 550 560 570 580 590 600 AENMAMSEWP LNNGHREDPA PGLLSEELLQ DEETLLSFMR DPSLRPGDPA RKARGRTRLP 610 620 630 640 650 660 AKKKPPPPPP QDGPGSRTTP DKAPKKTWGQ DAGSGKGGQG PPTRKPPRRT SSHLPSSPAA 670 680 690 700 710 720 GDCPILATPE SPPPLAPETP DEAASVAADS DVQVPGPAAS PKPLGRLRPP RESKVTRRLP 730 740 750 760 770 780 GARPDAGMGP PSAVAERPKV SLHFDTETDG YFSDGEMSDS DVEAEDGGVQ RGPREAGAEE VVRMGVLAS
	Isoform 3 [UniParc]. Checksum: 6C53A5098E9966BA	FASTA	562	63,466
	10 20 30 40 50 60 MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA 70 80 90 100 110 120 MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRILPPLE GPPAQASPSS 130 140 150 160 170 180 TMLGEGSQPD WPGGSRYDLD EIDAYWLELI NSELKEMERP ELDELTLERV LEELETLCHQ 190 200 210 220 230 240 NMARAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS 250 260 270 280 290 300 WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS 310 320 330 340 350 360 HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA DNDEVKFKSF 370 380 390 400 410 420 CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL EEDFYELVEP AEVAERLDLA 430 440 450 460 470 480 EALVDFIYQY WKLKRKANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR 490 500 510 520 530 540 NLCYMVTRRE RTKHAICKLQ EQIFHLQMKL IEQDLCRERS GRRAKGKKSD SKRKGCEGSK 550 560 GSTEKKEKVK AGPDSVLGQL GE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Bone marrow.
[2]	"Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones." Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N., Ohara O. DNA Res. 10:49-57(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Melanoma and Uterus.
[4]	"Isolation and characterisation of a 64 kDa protein associated with the lysosomal/autophagic compartment." Tesmann S., Biederbick A., Elsaesser H.P. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 202-790 (ISOFORM 1).
[5]	"Identification of Jade1, a gene encoding a PHD zinc finger protein, in a gene trap mutagenesis screen for genes involved in anteroposterior axis development." Tzouanacou E., Tweedie S., Wilson V. Mol. Cell. Biol. 23:8553-8562(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation." Doyon Y., Cayrou C., Ullah M., Landry A.-J., Cote V., Selleck W., Lane W.S., Tan S., Yang X.-J., Cote J. Mol. Cell 21:51-64(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN HISTONE H4 ACETYLATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE HBO1 COMPLEX.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-32; LYS-38 AND LYS-298, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87076 mRNA. Translation: BAA13245.2. Different initiation. AK074123 mRNA. Translation: BAB84949.1. Frameshift. BC009307 mRNA. Translation: AAH09307.2. BC021962 mRNA. Translation: AAH21962.2. AJ251833 mRNA. Translation: CAB94935.1. Different initiation. BN000288 mRNA. Translation: CAE30501.1.
IPI	IPI00792658. IPI00878910. IPI00884143.
RefSeq	NP_056103.4. NM_015288.4.
UniGene	Hs.732155.
3D structure databases
ProteinModelPortal	Q9NQC1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NQC1. 7 interactions.
STRING	9606.ENSP00000378451.
Polymorphism databases
DMDM	116242597.
Proteomic databases
PaxDb	Q9NQC1.
PRIDE	Q9NQC1.
Protocols and materials databases
DNASU	23338.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000395003; ENSP00000378451; ENSG00000043143.
GeneID	23338.
KEGG	hsa:23338.
UCSC	uc003kzl.2. human. uc003kzo.1. human.
Organism-specific databases
CTD	23338.
GeneCards	GC05P133860.
H-InvDB	HIX0005181.
HGNC	HGNC:22984. PHF15.
HPA	HPA025959.
MIM	610515. gene.
neXtProt	NX_Q9NQC1.
PharmGKB	PA128394627.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5141.
HOGENOM	HOG000220882.
HOVERGEN	HBG053585.
PhylomeDB	Q9NQC1.
Gene expression databases
ArrayExpress	Q9NQC1.
Bgee	Q9NQC1.
CleanEx	HS_PHF15.
Genevestigator	Q9NQC1.
GermOnline	ENSG00000043143. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR019542. Enhancer_polycomb-like_N. IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF10513. EPL1. 1 hit. [Graphical view]
SMART	SM00249. PHD. 2 hits. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PHF15. human.
GenomeRNAi	23338.
NextBio	45289.
SOURCE	Search...

Entry information

Entry name

JADE2_HUMAN

Accession

Primary (citable) accession number: Q9NQC1
Secondary accession number(s): Q6IE80 , Q8TEK0, Q92513, Q96GQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.