Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase Jade-2

Gene

JADE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo (PubMed:16387653). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed:25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 249PHD-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri251 – 285C2HC pre-PHD-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri309 – 365PHD-type 2PROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

GO - Biological processi

  • histone H3 acetylation Source: UniProtKB
  • histone H4-K12 acetylation Source: UniProtKB
  • histone H4-K5 acetylation Source: UniProtKB
  • histone H4-K8 acetylation Source: UniProtKB
  • protein ubiquitination Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3214847 HATs acetylate histones
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Jade-21 Publication (EC:2.3.2.271 Publication)
Alternative name(s):
Jade family PHD finger protein 2
PHD finger protein 15
Gene namesi
Name:JADE2
Synonyms:KIAA0239, PHF15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000043143.20
HGNCiHGNC:22984 JADE2
MIMi610515 gene
neXtProtiNX_Q9NQC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi202C → A: Loss of E3 ubiquitin-protein ligase activity on KDM1A. 1 Publication1
Mutagenesisi243C → A: Loss of E3 ubiquitin-protein ligase activity on KDM1A. 1 Publication1

Organism-specific databases

DisGeNETi23338
OpenTargetsiENSG00000043143
PharmGKBiPA128394627

Polymorphism and mutation databases

BioMutaiPHF15
DMDMi116242597

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000593081 – 790E3 ubiquitin-protein ligase Jade-2Add BLAST790

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei32N6-acetyllysineCombined sources1
Modified residuei38N6-acetyllysineCombined sources1
Modified residuei117PhosphoserineBy similarity1
Modified residuei298N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9NQC1
PaxDbiQ9NQC1
PeptideAtlasiQ9NQC1
PRIDEiQ9NQC1
ProteomicsDBi82130
82131 [Q9NQC1-2]
82132 [Q9NQC1-3]

PTM databases

iPTMnetiQ9NQC1
PhosphoSitePlusiQ9NQC1

Expressioni

Gene expression databases

BgeeiENSG00000043143
CleanExiHS_PHF15
ExpressionAtlasiQ9NQC1 baseline and differential
GenevisibleiQ9NQC1 HS

Organism-specific databases

HPAiHPA025959
HPA055789

Interactioni

Subunit structurei

Component of the HBO1 complex composed at least of ING4 or ING5, MYST2/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3 (PubMed:16387653). Interacts (via C-terminus) with KDM1A (via AOD/Tower domain) (PubMed:25018020).2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi116924, 25 interactors
ComplexPortaliCPX-719 HBO1-4.2 histone acetyltransferase complex
CPX-722 HBO1-5.2 histone acetyltransferase complex
CORUMiQ9NQC1
IntActiQ9NQC1, 16 interactors
STRINGi9606.ENSP00000378451

Structurei

3D structure databases

ProteinModelPortaliQ9NQC1
SMRiQ9NQC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi601 – 732Pro-richAdd BLAST132

Domaini

The first PHD domain is essential for its E3 ubiquitin ligase activity.1 Publication

Sequence similaritiesi

Belongs to the JADE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 249PHD-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri251 – 285C2HC pre-PHD-typePROSITE-ProRule annotationAdd BLAST35
Zinc fingeri309 – 365PHD-type 2PROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0954 Eukaryota
COG5141 LUCA
GeneTreeiENSGT00740000114866
HOGENOMiHOG000220882
HOVERGENiHBG053585
InParanoidiQ9NQC1
KOiK22155
PhylomeDBiQ9NQC1
TreeFamiTF316118

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR019542 Enhancer_polycomb-like_N
IPR034732 EPHD
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF10513 EPL1, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51805 EPHD, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NQC1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS
60 70 80 90 100
EVFRTDLITA MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE
110 120 130 140 150
PVVRILPPLE GPPAQASPSS TMLGEGSQPD WPGGSRYDLD EIDAYWLELI
160 170 180 190 200
NSELKEMERP ELDELTLERV LEELETLCHQ NMARAIETQE GLGIEYDEDV
210 220 230 240 250
VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS WLCRTCALGV
260 270 280 290 300
QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS
310 320 330 340 350
HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA
360 370 380 390 400
DNDEVKFKSF CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL
410 420 430 440 450
EEDFYELVEP AEVAERLDLA EALVDFIYQY WKLKRKANAN QPLLTPKTDE
460 470 480 490 500
VDNLAQQEQD VLYRRLKLFT HLRQDLERVR NLCYMVTRRE RTKHAICKLQ
510 520 530 540 550
EQIFHLQMKL IEQDLCRGLS TSFPIDGTFF NSWLAQSVQI TAENMAMSEW
560 570 580 590 600
PLNNGHREDP APGLLSEELL QDEETLLSFM RDPSLRPGDP ARKARGRTRL
610 620 630 640 650
PAKKKPPPPP PQDGPGSRTT PDKAPKKTWG QDAGSGKGGQ GPPTRKPPRR
660 670 680 690 700
TSSHLPSSPA AGDCPILATP ESPPPLAPET PDEAASVAAD SDVQVPGPAA
710 720 730 740 750
SPKPLGRLRP PRESKVTRRL PGARPDAGMG PPSAVAERPK VSLHFDTETD
760 770 780 790
GYFSDGEMSD SDVEAEDGGV QRGPREAGAE EVVRMGVLAS
Length:790
Mass (Da):87,466
Last modified:October 17, 2006 - v2
Checksum:i22C1B1A0360BC1AE
GO
Isoform 2 (identifier: Q9NQC1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     518-518: Missing.

Show »
Length:789
Mass (Da):87,409
Checksum:iEFCA863CE55B6297
GO
Isoform 3 (identifier: Q9NQC1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     518-790: GLSTSFPIDG...EVVRMGVLAS → ERSGRRAKGK...PDSVLGQLGE

Note: No experimental confirmation available.
Show »
Length:562
Mass (Da):63,466
Checksum:i6C53A5098E9966BA
GO

Sequence cautioni

The sequence BAA13245 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB84949 differs from that shown. Reason: Frameshift at position 11.Curated
The sequence CAB94935 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331T → I in BAA13245 (PubMed:9039502).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053778581R → G. Corresponds to variant dbSNP:rs34200923Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021050518 – 790GLSTS…GVLAS → ERSGRRAKGKKSDSKRKGCE GSKGSTEKKEKVKAGPDSVL GQLGE in isoform 3. 1 PublicationAdd BLAST273
Alternative sequenceiVSP_021051518Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87076 mRNA Translation: BAA13245.2 Different initiation.
AK074123 mRNA Translation: BAB84949.1 Frameshift.
BC009307 mRNA Translation: AAH09307.2
BC021962 mRNA Translation: AAH21962.2
AC005355 Genomic DNA No translation available.
AC106763 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62253.1
CH471062 Genomic DNA Translation: EAW62254.1
AJ251833 mRNA Translation: CAB94935.1 Different initiation.
BN000288 mRNA Translation: CAE30501.1
CCDSiCCDS4176.1 [Q9NQC1-1]
RefSeqiNP_001276913.1, NM_001289984.1
NP_001276914.1, NM_001289985.1
NP_001295072.1, NM_001308143.1
NP_056103.4, NM_015288.5 [Q9NQC1-1]
UniGeneiHs.732155

Genome annotation databases

EnsembliENST00000395003; ENSP00000378451; ENSG00000043143 [Q9NQC1-1]
GeneIDi23338
KEGGihsa:23338
UCSCiuc003kzm.3 human [Q9NQC1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiJADE2_HUMAN
AccessioniPrimary (citable) accession number: Q9NQC1
Secondary accession number(s): D3DQA3
, Q6IE80, Q8TEK0, Q92513, Q96GQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 17, 2006
Last modified: June 20, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health