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Reviewed, UniProtKB/Swiss-Prot Q9NQ90 (ANO2_HUMAN)

Last modified November 24, 2009. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Anoctamin-2
Alternative name(s):
    Transmembrane protein 16B
Gene names
Name: ANO2
Synonyms: C12orf3, TMEM16B
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1003 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina By similarity.

Subunit structure

Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminus region By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Retina, especially in the photoreceptor synaptic terminals. Ref.2

Post-translational modification

Glycosylated By similarity.

Sequence similarities

Belongs to the anoctamin family.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
   LigandCalcium
Chloride
   Molecular functionChloride channel
Ionic channel
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological procession transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloride channel complex

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

chloride ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular calcium activated chloride channel activity

Inferred from direct assay. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NQ90-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NQ90-2)

The sequence of this isoform differs from the canonical sequence as follows:
     5-7: GPR → MPE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10031003Anoctamin-2
PRO_0000072564

Regions

Topological domain1 – 365365Cytoplasmic Potential
Transmembrane366 – 38621 Potential
Topological domain387 – 43448Extracellular Potential
Transmembrane435 – 45521 Potential
Topological domain456 – 53883Cytoplasmic Potential
Transmembrane539 – 55921 Potential
Topological domain560 – 58223Extracellular Potential
Transmembrane583 – 60321 Potential
Topological domain604 – 62320Cytoplasmic Potential
Transmembrane624 – 64421 Potential
Topological domain645 – 748104Extracellular Potential
Transmembrane749 – 76921 Potential
Topological domain770 – 80132Cytoplasmic Potential
Transmembrane802 – 82221 Potential
Topological domain823 – 90785Extracellular Potential
Transmembrane908 – 92821 Potential
Topological domain929 – 100375Cytoplasmic Potential
Motif1001 – 10033DLG4 binding (PDZ)

Amino acid modifications

Glycosylation4221N-linked (GlcNAc...) Potential
Glycosylation8411N-linked (GlcNAc...) Potential
Glycosylation8491N-linked (GlcNAc...) Potential
Glycosylation8561N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence5 – 73GPR → MPE in isoform 2.
VSP_038352
Natural variant1121V → A: dbSNP rs3741903.
VAR_021932
Natural variant1471P → S: dbSNP rs3741901.
VAR_057286
Natural variant5051S → A: dbSNP rs1860961.
VAR_020331

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 3, 2009. Version 2.
Checksum: 32939EADC8AFE5C8

FASTA1,003113,969
        10         20         30         40         50         60 
MATPGPRDIP LLPGSPRRLS PQAGSRGGQG PKHGQQCLKM PGPRAPGLQG GSNRDPGQPC 

        70         80         90        100        110        120 
GGESTRSSSV INNYLDANEP VSLEARLSRM HFHDSQRKVD YVLAYHYRKR GVHLAQGFPG 

       130        140        150        160        170        180 
HSLAIVSNGE TGKEPHAGGP GDIELGPLDA LEEERKEQRE EFEHNLMEAG LELEKDLENK 

       190        200        210        220        230        240 
SQGSIFVRIH APWQVLAREA EFLKIKVPTK KEMYEIKAGG SIAKKFSAAL QKLSSHLQPR 

       250        260        270        280        290        300 
VPEHSNNKMK NLSYPFSREK MYLYNIQEKD TFFDNATRSR IVHEILKRTA CSRANNTMGI 

       310        320        330        340        350        360 
NSLIANNIYE AAYPLHDGEY DSPEDDMNDR KLLYQEWARY GVFYKFQPID LIRKYFGEKI 

       370        380        390        400        410        420 
GLYFAWLGLY TSFLIPSSVI GVIVFLYGCA TIEEDIPSRE MCDQQNAFTM CPLCDKSCDY 

       430        440        450        460        470        480 
WNLSSACGTA QASHLFDNPA TVFFSIFMAL WATMFLENWK RLQMRLGYFW DLTGIEEEEE 

       490        500        510        520        530        540 
RAQEHSRPEY ETKVREKMLK ESNQSAVQKL ETNTTECGDE DDEDKLTWKD RFPGYLMNFA 

       550        560        570        580        590        600 
SILFMIALTF SIVFGVIVYR ITTAAALSLN KATRSNVRVT VTATAVIINL VVILILDEIY 

       610        620        630        640        650        660 
GAVAKWLTKI EVPKTEQTFE ERLILKAFLL KFVNAYSPIF YVAFFKGRFV GRPGSYVYVF 

       670        680        690        700        710        720 
DGYRMEECAP GGCLMELCIQ LSIIMLGKQL IQNNIFEIGV PKLKKLFRKL KDETEAGETD 

       730        740        750        760        770        780 
SAHSKHPEQW DLDYSLEPYT GLTPEYMEMI IQFGFVTLFV ASFPLAPVFA LLNNVIEVRL 

       790        800        810        820        830        840 
DAKKFVTELR RPDAVRTKDI GIWFDILSGI GKFSVISNAF VIAITSDFIP RLVYQYSYSH 

       850        860        870        880        890        900 
NGTLHGFVNH TLSFFNVSQL KEGTQPENSQ FDQEVQFCRF KDYREPPWAP NPYEFSKQYW 

       910        920        930        940        950        960 
FILSARLAFV IIFQNLVMFL SVLVDWMIPD IPTDISDQIK KEKSLLVDFF LKEEHEKLKL 

       970        980        990       1000 
MDEPALRSPG GGDRSRSRAA SSAPSGQSQL GSMMSSGSQH TNV 

« Hide

Isoform 2.

Checksum: D6DD526105EE1596
Show »

FASTA1,003114,016

References

« Hide 'large scale' references
[1]"Transcripts in 12p13.3."
Lorenz B., White K.E., Econs M.J., Strom T.M.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Retina.
[2]"TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals."
Stoehr H., Heisig J.B., Benz P.M., Schoeberl S., Milenkovic V.M., Strauss O., Aartsen W.M., Wijnholds J., Weber B.H.F., Schulz H.L.
J. Neurosci. 29:6809-6818(2009) [PubMed: 19474308] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, GLYCOSYLATION, SUBUNIT, TISSUE SPECIFICITY.
Tissue: Retina.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 556-999 (ISOFORMS 1 AND 2).
Tissue: Retinoblastoma.
[4]"TMEM16A confers receptor-activated calcium-dependent chloride conductance."
Yang Y.D., Cho H., Koo J.Y., Tak M.H., Cho Y., Shim W.-S., Park S.P., Lee J., Lee B., Kim B.-M., Raouf R., Shin Y.K., Oh U.
Nature 455:1210-1215(2008) [PubMed: 18724360] [Abstract]
Cited for: IDENTIFICATION AS PUTATIVE CHLORIDE CHANNEL.
+Additional computationally mapped references.

Cross-references

Sequence databases

AJ272204 mRNA. Translation: CAC01125.1.
FJ384095 mRNA. Translation: ACL36050.1.
AK024010 mRNA. Translation: BAB14773.1. Different initiation.
IPIIPI00479837.
RefSeqNP_065106.2.
UniGeneHs.148970

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9NQ90.

PTM databases

PhosphoSiteQ9NQ90.

Genome annotation databases

EnsemblENST00000356134; ENSP00000348453; ENSG00000047617; Homo sapiens. [Genome view]
GeneID57101.
KEGGhsa:57101.

Organism-specific databases

CTD57101.
GeneCardsGC12M005543.
HGNCHGNC:1183. ANO2.
MIM610109. gene.
PharmGKBPA25504.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9NQ90.
HOVERGENQ9NQ90.
OMADEDKLTW

Enzyme and pathway databases

BioCycCATTLE:ENSBTAG00000012246-MON.

Gene expression databases

ArrayExpressQ9NQ90.
BgeeQ9NQ90.
CleanExHS_ANO2.
GenevestigatorQ9NQ90.
GermOnlineENSG00000047617. Homo sapiens.

Family and domain databases

InterProIPR007632. DUF590.
[Graphical view]
PANTHERPTHR12308. DUF590. 1 hit.
PfamPF04547. DUF590. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio62929.
SOURCESearch...

Entry information

Entry nameANO2_HUMAN
AccessionPrimary (citable) accession number: Q9NQ90
Secondary accession number(s): C4N787, Q9H847
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 3, 2009
Last modified: November 24, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents