Reviewed, UniProtKB/Swiss-Prot Q9NQ90 (ANO2_HUMAN)

Names and origin

Protein names

Recommended name:
    Anoctamin-2
Alternative name(s):
    Transmembrane protein 16B

Gene names

Name:	ANO2
Synonyms:	C12orf3, TMEM16B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1003 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina By similarity.
Subunit structure	Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminus region By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Retina, especially in the photoreceptor synaptic terminals. Ref.2
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the anoctamin family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	Calcium Chloride
Molecular function	Chloride channel Ionic channel
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW intracellular calcium activated chloride channel activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQ90-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQ90-2)

The sequence of this isoform differs from the canonical sequence as follows:
     5-7: GPR → MPE

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1003	1003	Anoctamin-2		PRO_0000072564
Regions
	Topological domain	1 – 365	365	Cytoplasmic Potential
	Transmembrane	366 – 386	21	Potential
	Topological domain	387 – 434	48	Extracellular Potential
	Transmembrane	435 – 455	21	Potential
	Topological domain	456 – 538	83	Cytoplasmic Potential
	Transmembrane	539 – 559	21	Potential
	Topological domain	560 – 582	23	Extracellular Potential
	Transmembrane	583 – 603	21	Potential
	Topological domain	604 – 623	20	Cytoplasmic Potential
	Transmembrane	624 – 644	21	Potential
	Topological domain	645 – 748	104	Extracellular Potential
	Transmembrane	749 – 769	21	Potential
	Topological domain	770 – 801	32	Cytoplasmic Potential
	Transmembrane	802 – 822	21	Potential
	Topological domain	823 – 907	85	Extracellular Potential
	Transmembrane	908 – 928	21	Potential
	Topological domain	929 – 1003	75	Cytoplasmic Potential
	Motif	1001 – 1003	3	DLG4 binding (PDZ)
Amino acid modifications
	Glycosylation	422	1	N-linked (GlcNAc...) Potential
	Glycosylation	841	1	N-linked (GlcNAc...) Potential
	Glycosylation	849	1	N-linked (GlcNAc...) Potential
	Glycosylation	856	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	5 – 7	3	GPR → MPE in isoform 2.		VSP_038352
	Natural variant	112	1	V → A: dbSNP rs3741903.		VAR_021932
	Natural variant	147	1	P → S: dbSNP rs3741901.		VAR_057286
	Natural variant	505	1	S → A: dbSNP rs1860961.		VAR_020331

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 3, 2009. Version 2. Checksum: 32939EADC8AFE5C8	FASTA	1,003	113,969
	10 20 30 40 50 60 MATPGPRDIP LLPGSPRRLS PQAGSRGGQG PKHGQQCLKM PGPRAPGLQG GSNRDPGQPC 70 80 90 100 110 120 GGESTRSSSV INNYLDANEP VSLEARLSRM HFHDSQRKVD YVLAYHYRKR GVHLAQGFPG 130 140 150 160 170 180 HSLAIVSNGE TGKEPHAGGP GDIELGPLDA LEEERKEQRE EFEHNLMEAG LELEKDLENK 190 200 210 220 230 240 SQGSIFVRIH APWQVLAREA EFLKIKVPTK KEMYEIKAGG SIAKKFSAAL QKLSSHLQPR 250 260 270 280 290 300 VPEHSNNKMK NLSYPFSREK MYLYNIQEKD TFFDNATRSR IVHEILKRTA CSRANNTMGI 310 320 330 340 350 360 NSLIANNIYE AAYPLHDGEY DSPEDDMNDR KLLYQEWARY GVFYKFQPID LIRKYFGEKI 370 380 390 400 410 420 GLYFAWLGLY TSFLIPSSVI GVIVFLYGCA TIEEDIPSRE MCDQQNAFTM CPLCDKSCDY 430 440 450 460 470 480 WNLSSACGTA QASHLFDNPA TVFFSIFMAL WATMFLENWK RLQMRLGYFW DLTGIEEEEE 490 500 510 520 530 540 RAQEHSRPEY ETKVREKMLK ESNQSAVQKL ETNTTECGDE DDEDKLTWKD RFPGYLMNFA 550 560 570 580 590 600 SILFMIALTF SIVFGVIVYR ITTAAALSLN KATRSNVRVT VTATAVIINL VVILILDEIY 610 620 630 640 650 660 GAVAKWLTKI EVPKTEQTFE ERLILKAFLL KFVNAYSPIF YVAFFKGRFV GRPGSYVYVF 670 680 690 700 710 720 DGYRMEECAP GGCLMELCIQ LSIIMLGKQL IQNNIFEIGV PKLKKLFRKL KDETEAGETD 730 740 750 760 770 780 SAHSKHPEQW DLDYSLEPYT GLTPEYMEMI IQFGFVTLFV ASFPLAPVFA LLNNVIEVRL 790 800 810 820 830 840 DAKKFVTELR RPDAVRTKDI GIWFDILSGI GKFSVISNAF VIAITSDFIP RLVYQYSYSH 850 860 870 880 890 900 NGTLHGFVNH TLSFFNVSQL KEGTQPENSQ FDQEVQFCRF KDYREPPWAP NPYEFSKQYW 910 920 930 940 950 960 FILSARLAFV IIFQNLVMFL SVLVDWMIPD IPTDISDQIK KEKSLLVDFF LKEEHEKLKL 970 980 990 1000 MDEPALRSPG GGDRSRSRAA SSAPSGQSQL GSMMSSGSQH TNV
	Isoform 2. Checksum: D6DD526105EE1596	FASTA	1,003	114,016
	10 20 30 40 50 60 MATPMPEDIP LLPGSPRRLS PQAGSRGGQG PKHGQQCLKM PGPRAPGLQG GSNRDPGQPC 70 80 90 100 110 120 GGESTRSSSV INNYLDANEP VSLEARLSRM HFHDSQRKVD YVLAYHYRKR GVHLAQGFPG 130 140 150 160 170 180 HSLAIVSNGE TGKEPHAGGP GDIELGPLDA LEEERKEQRE EFEHNLMEAG LELEKDLENK 190 200 210 220 230 240 SQGSIFVRIH APWQVLAREA EFLKIKVPTK KEMYEIKAGG SIAKKFSAAL QKLSSHLQPR 250 260 270 280 290 300 VPEHSNNKMK NLSYPFSREK MYLYNIQEKD TFFDNATRSR IVHEILKRTA CSRANNTMGI 310 320 330 340 350 360 NSLIANNIYE AAYPLHDGEY DSPEDDMNDR KLLYQEWARY GVFYKFQPID LIRKYFGEKI 370 380 390 400 410 420 GLYFAWLGLY TSFLIPSSVI GVIVFLYGCA TIEEDIPSRE MCDQQNAFTM CPLCDKSCDY 430 440 450 460 470 480 WNLSSACGTA QASHLFDNPA TVFFSIFMAL WATMFLENWK RLQMRLGYFW DLTGIEEEEE 490 500 510 520 530 540 RAQEHSRPEY ETKVREKMLK ESNQSAVQKL ETNTTECGDE DDEDKLTWKD RFPGYLMNFA 550 560 570 580 590 600 SILFMIALTF SIVFGVIVYR ITTAAALSLN KATRSNVRVT VTATAVIINL VVILILDEIY 610 620 630 640 650 660 GAVAKWLTKI EVPKTEQTFE ERLILKAFLL KFVNAYSPIF YVAFFKGRFV GRPGSYVYVF 670 680 690 700 710 720 DGYRMEECAP GGCLMELCIQ LSIIMLGKQL IQNNIFEIGV PKLKKLFRKL KDETEAGETD 730 740 750 760 770 780 SAHSKHPEQW DLDYSLEPYT GLTPEYMEMI IQFGFVTLFV ASFPLAPVFA LLNNVIEVRL 790 800 810 820 830 840 DAKKFVTELR RPDAVRTKDI GIWFDILSGI GKFSVISNAF VIAITSDFIP RLVYQYSYSH 850 860 870 880 890 900 NGTLHGFVNH TLSFFNVSQL KEGTQPENSQ FDQEVQFCRF KDYREPPWAP NPYEFSKQYW 910 920 930 940 950 960 FILSARLAFV IIFQNLVMFL SVLVDWMIPD IPTDISDQIK KEKSLLVDFF LKEEHEKLKL 970 980 990 1000 MDEPALRSPG GGDRSRSRAA SSAPSGQSQL GSMMSSGSQH TNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcripts in 12p13.3." Lorenz B., White K.E., Econs M.J., Strom T.M. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Retina.
[2]	"TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals." Stoehr H., Heisig J.B., Benz P.M., Schoeberl S., Milenkovic V.M., Strauss O., Aartsen W.M., Wijnholds J., Weber B.H.F., Schulz H.L. J. Neurosci. 29:6809-6818(2009) [PubMed: 19474308] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, GLYCOSYLATION, SUBUNIT, TISSUE SPECIFICITY. Tissue: Retina.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 556-999 (ISOFORMS 1 AND 2). Tissue: Retinoblastoma.
[4]	"TMEM16A confers receptor-activated calcium-dependent chloride conductance." Yang Y.D., Cho H., Koo J.Y., Tak M.H., Cho Y., Shim W.-S., Park S.P., Lee J., Lee B., Kim B.-M., Raouf R., Shin Y.K., Oh U. Nature 455:1210-1215(2008) [PubMed: 18724360] [Abstract] Cited for: IDENTIFICATION AS PUTATIVE CHLORIDE CHANNEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ272204 mRNA. Translation: CAC01125.1. FJ384095 mRNA. Translation: ACL36050.1. AK024010 mRNA. Translation: BAB14773.1. Different initiation.
IPI	IPI00479837.
RefSeq	NP_065106.2.
UniGene	Hs.148970
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NQ90.
PTM databases
PhosphoSite	Q9NQ90.
Genome annotation databases
Ensembl	ENST00000356134; ENSP00000348453; ENSG00000047617; Homo sapiens. [Genome view]
GeneID	57101.
KEGG	hsa:57101.
Organism-specific databases
CTD	57101.
GeneCards	GC12M005543.
HGNC	HGNC:1183. ANO2.
MIM	610109. gene.
PharmGKB	PA25504.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NQ90.
HOVERGEN	Q9NQ90.
OMA	DEDKLTW
Enzyme and pathway databases
BioCyc	CATTLE:ENSBTAG00000012246-MON.
Gene expression databases
ArrayExpress	Q9NQ90.
Bgee	Q9NQ90.
CleanEx	HS_ANO2.
Genevestigator	Q9NQ90.
GermOnline	ENSG00000047617. Homo sapiens.
Family and domain databases
InterPro	IPR007632. DUF590. [Graphical view]
PANTHER	PTHR12308. DUF590. 1 hit.
Pfam	PF04547. DUF590. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	62929.
SOURCE	Search...

Entry information

Entry name

ANO2_HUMAN

Accession

Primary (citable) accession number: Q9NQ90
Secondary accession number(s): C4N787, Q9H847

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 3, 2009
Last modified:	November 24, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.