Reviewed, UniProtKB/Swiss-Prot Q9NQ84 (GPC5C_HUMAN)

Names and origin

Protein names

Recommended name:
    G-protein coupled receptor family C group 5 member C
Alternative name(s):
    Retinoic acid-induced gene 3 protein
      Short name=RAIG-3

Gene names

Name:	GPRC5C
Synonyms:	RAIG3
ORF Names:	PSEC0087

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Localized in the plasma membrane and perinuclear vesicles. Ref.1 Ref.2
Tissue specificity	Expression is highest in the periphery, particularly in the stomach, but also in the kidney, liver, pancreas, and prostate. In brain, levels of expression are generally lower than in the periphery, with the exception of cerebellum, spinal cord, and dorsal root ganglia (DRG). Ref.1 Ref.2
Induction	By all-trans retinoic acid (ATRA). Ref.1 Ref.2
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.11
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Traceable author statement. Source: ProtInc
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	G-protein coupled receptor activity Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BAT5	O95870	1	EBI-348052,EBI-348517

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQ84-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQ84-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGTQPEPGLGARM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 441	418	G-protein coupled receptor family C group 5 member C		PRO_0000012967
Regions
	Topological domain	24 – 50	27	Extracellular Potential
	Transmembrane	51 – 71	21	1 Potential
	Topological domain	72 – 85	14	Cytoplasmic Potential
	Transmembrane	86 – 106	21	2 Potential
	Topological domain	107 – 120	14	Extracellular Potential
	Transmembrane	121 – 141	21	3 Potential
	Topological domain	142 – 155	14	Cytoplasmic Potential
	Transmembrane	156 – 176	21	4 Potential
	Topological domain	177 – 208	32	Extracellular Potential
	Transmembrane	209 – 229	21	5 Potential
	Topological domain	230 – 241	12	Cytoplasmic Potential
	Transmembrane	242 – 262	21	6 Potential
	Topological domain	263 – 279	17	Extracellular Potential
	Transmembrane	280 – 300	21	7 Potential
	Topological domain	301 – 441	141	Cytoplasmic Potential
Amino acid modifications
	Modified residue	324	1	Phosphotyrosine Ref.10
	Modified residue	398	1	Phosphoserine Ref.11
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1	1	M → MGTQPEPGLGARM in isoform 2.		VSP_037725
Experimental info
	Sequence conflict	204	1	I → V in CAC00633. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: B255D9680328FE3D	FASTA	441	48,193
	10 20 30 40 50 60 MAIHKALVMC LGLPLFLFPG AWAQGHVPPG CSQGLNPLYY NLCDRSGAWG IVLEAVAGAG 70 80 90 100 110 120 IVTTFVLTII LVASLPFVQD TKKRSLLGTQ VFFLLGTLGL FCLVFACVVK PDFSTCASRR 130 140 150 160 170 180 FLFGVLFAIC FSCLAAHVFA LNFLARKNHG PRGWVIFTVA LLLTLVEVII NTEWLIITLV 190 200 210 220 230 240 RGSGEGGPQG NSSAGWAVAS PCAIANMDFV MALIYVMLLL LGAFLGAWPA LCGRYKRWRK 250 260 270 280 290 300 HGVFVLLTTA TSVAIWVVWI VMYTYGNKQH NSPTWDDPTL AIALAANAWA FVLFYVIPEV 310 320 330 340 350 360 SQVTKSSPEQ SYQGDMYPTR GVGYETILKE QKGQSMFVEN KAFSMDEPVA AKRPVSPYSG 370 380 390 400 410 420 YNGQLLTSVY QPTEMALMHK VPSEGAYDII LPRATANSQV MGSANSTLRA EDMYSAQSHQ 430 440 AATPPKDGKN SQVFRNPYVW D
	Isoform 2. Checksum: 232D2F2BCD64EE46	FASTA	453	49,389
	10 20 30 40 50 60 MGTQPEPGLG ARMAIHKALV MCLGLPLFLF PGAWAQGHVP PGCSQGLNPL YYNLCDRSGA 70 80 90 100 110 120 WGIVLEAVAG AGIVTTFVLT IILVASLPFV QDTKKRSLLG TQVFFLLGTL GLFCLVFACV 130 140 150 160 170 180 VKPDFSTCAS RRFLFGVLFA ICFSCLAAHV FALNFLARKN HGPRGWVIFT VALLLTLVEV 190 200 210 220 230 240 IINTEWLIIT LVRGSGEGGP QGNSSAGWAV ASPCAIANMD FVMALIYVML LLLGAFLGAW 250 260 270 280 290 300 PALCGRYKRW RKHGVFVLLT TATSVAIWVV WIVMYTYGNK QHNSPTWDDP TLAIALAANA 310 320 330 340 350 360 WAFVLFYVIP EVSQVTKSSP EQSYQGDMYP TRGVGYETIL KEQKGQSMFV ENKAFSMDEP 370 380 390 400 410 420 VAAKRPVSPY SGYNGQLLTS VYQPTEMALM HKVPSEGAYD IILPRATANS QVMGSANSTL 430 440 450 RAEDMYSAQS HQAATPPKDG KNSQVFRNPY VWD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)." Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N. Genomics 67:8-18(2000) [PubMed: 10945465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[2]	"Cloning and characterization of a human orphan family C G-protein coupled receptor GPRC5D." Braeuner-Osborne H., Jensen A.A., Sheppard P.O., Brodin B., Krogsgaard-Larsen P., O'Hara P. Biochim. Biophys. Acta 1518:237-248(2001) [PubMed: 11311935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	"Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N. Nat. Methods 5:1011-1017(2008) [PubMed: 19054851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary and Pancreas.
[10]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-324, MASS SPECTROMETRY.
[11]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-398, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ276102 mRNA. Translation: CAC00633.1. Different initiation. AF207989 mRNA. Translation: AAF72870.1. BT007435 mRNA. Translation: AAP36103.1. AK131210 mRNA. Translation: BAG54752.1. Different initiation. AK075397 mRNA. Translation: BAC11595.1. AB451470 mRNA. Translation: BAG70284.1. AC079325 Genomic DNA. No translation available. CH471099 Genomic DNA. Translation: EAW89163.1. BC016860 mRNA. Translation: AAH16860.2. Different initiation. BC110848 mRNA. Translation: AAI10849.1. Different initiation. BC125080 mRNA. Translation: AAI25081.1. Different initiation. BC125081 mRNA. Translation: AAI25082.1. Different initiation.
IPI	IPI00099883. IPI00789902.
RefSeq	NP_061123.3. NP_071319.2.
UniGene	Hs.446438
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NQ84. 3 interactions.
STRING	Q9NQ84.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9NQ84.
Proteomic databases
PRIDE	Q9NQ84.
Genome annotation databases
Ensembl	ENST00000342648; ENSP00000340595; ENSG00000170412; Homo sapiens. [Genome view] ENST00000392627; ENSP00000376403; ENSG00000170412; Homo sapiens. [Genome view] ENST00000392629; ENSP00000376405; ENSG00000170412; Homo sapiens. [Genome view] ENST00000430649; ENSP00000412385; ENSG00000170412; Homo sapiens. [Genome view]
GeneID	55890.
KEGG	hsa:55890.
UCSC	uc002jkp.1. human.
Organism-specific databases
CTD	55890.
GeneCards	GC17P069938.
H-InvDB	HIX0014144.
HGNC	HGNC:13309. GPRC5C.
MIM	605949. gene.
PharmGKB	PA28939.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG445102.
HOVERGEN	Q9NQ84.
InParanoid	Q9NQ84.
OrthoDB	EOG9RBT43.
Gene expression databases
ArrayExpress	Q9NQ84.
Bgee	Q9NQ84.
CleanEx	HS_GPRC5C.
Genevestigator	Q9NQ84.
GermOnline	ENSG00000170412. Homo sapiens.
Family and domain databases
InterPro	IPR017978. GPCR_3_C. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. [Graphical view]
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. False negative. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	61227.
SOURCE	Search...

Entry information

Entry name

GPC5C_HUMAN

Accession

Primary (citable) accession number: Q9NQ84
Secondary accession number(s): B5BUN4, Q2NL85, Q9NZG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	December 15, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.