Q9NQ76 (MEPE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Matrix extracellular phosphoglycoprotein

Alternative name(s):
Osteoblast/osteocyte factor 45
Short name=OF45

Gene names

Name:

MEPE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes renal phosphate excretion and modulates mineralization. Ref.7
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Expressed by osteoblasts. Secreted from oncogenic hypophosphataemic tumors.
Post-translational modification	Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. Ref.8

Ontologies

Keywords
Biological process	Biomineralization
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biomineral tissue development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of bone mineralization Inferred from electronic annotation. Source: Compara skeletal system development Traceable author statement Ref.1. Source: ProtInc
Cellular_component	proteinaceous extracellular matrix Traceable author statement Ref.1. Source: ProtInc
Molecular_function	extracellular matrix structural constituent Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	3	EBI-1753293,EBI-389883

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQ76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQ76-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-36: R → RITYKGHYEKHGHYVFKCVYMSPEKKNQTDVK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 525	508	Matrix extracellular phosphoglycoprotein		PRO_0000021668
Regions
	Motif	247 – 249	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	477	1	N-linked (GlcNAc...) Potential
	Glycosylation	478	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	36	1	R → RITYKGHYEKHGHYVFKCVY MSPEKKNQTDVK in isoform 2.		VSP_043986
	Natural variant	330	1	V → I. Corresponds to variant rs17013285 [ dbSNP | Ensembl ].		VAR_034094

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0977CA6E871CA9E5	FASTA	525	58,419
	10 20 30 40 50 60 MRVFCVGLLL FSVTWAAPTF QPQTEKTKQS CVEEQRQEEK NKDNIGFHHL GKRINQELSS 70 80 90 100 110 120 KENIVQERKK DLSLSEASEN KGSSKSQNYF TNRQRLNKEY SISNKENTHN GLRMSIYPKS 130 140 150 160 170 180 TGNKGFEDGD DAISKLHDQE EYGAALIRNN MQHIMGPVTA IKLLGEENKE NTPRNVLNII 190 200 210 220 230 240 PASMNYAKAH SKDKKKPQRD SQAQKSPVKS KSTHRIQHNI DYLKHLSKVK KIPSDFEGSG 250 260 270 280 290 300 YTDLQERGDN DISPFSGDGQ PFKDIPGKGE ATGPDLEGKD IQTGFAGPSE AESTHLDTKK 310 320 330 340 350 360 PGYNEIPERE ENGGNTIGTR DETAKEADAV DVSLVEGSND IMGSTNFKEL PGREGNRVDA 370 380 390 400 410 420 GSQNAHQGKV EFHYPPAPSK EKRKEGSSDA AESTNYNEIP KNGKGSTRKG VDHSNRNQAT 430 440 450 460 470 480 LNEKQRFPSK GKSQGLPIPS RGLDNEIKNE MDSFNGPSHE NIITHGRKYH YVPHRQNNST 490 500 510 520 RNKGMPQGKG SWGRQPHSNR RFSSRRRDDS SESSDSGSSS ESDGD
	Isoform 2 [UniParc]. Checksum: F475015A4923DA71	FASTA	556	62,160
	10 20 30 40 50 60 MRVFCVGLLL FSVTWAAPTF QPQTEKTKQS CVEEQRITYK GHYEKHGHYV FKCVYMSPEK 70 80 90 100 110 120 KNQTDVKQEE KNKDNIGFHH LGKRINQELS SKENIVQERK KDLSLSEASE NKGSSKSQNY 130 140 150 160 170 180 FTNRQRLNKE YSISNKENTH NGLRMSIYPK STGNKGFEDG DDAISKLHDQ EEYGAALIRN 190 200 210 220 230 240 NMQHIMGPVT AIKLLGEENK ENTPRNVLNI IPASMNYAKA HSKDKKKPQR DSQAQKSPVK 250 260 270 280 290 300 SKSTHRIQHN IDYLKHLSKV KKIPSDFEGS GYTDLQERGD NDISPFSGDG QPFKDIPGKG 310 320 330 340 350 360 EATGPDLEGK DIQTGFAGPS EAESTHLDTK KPGYNEIPER EENGGNTIGT RDETAKEADA 370 380 390 400 410 420 VDVSLVEGSN DIMGSTNFKE LPGREGNRVD AGSQNAHQGK VEFHYPPAPS KEKRKEGSSD 430 440 450 460 470 480 AAESTNYNEI PKNGKGSTRK GVDHSNRNQA TLNEKQRFPS KGKSQGLPIP SRGLDNEIKN 490 500 510 520 530 540 EMDSFNGPSH ENIITHGRKY HYVPHRQNNS TRNKGMPQGK GSWGRQPHSN RRFSSRRRDD 550 SSESSDSGSS SESDGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MEPE, a new gene expressed in bone marrow and tumors causing osteomalacia." Rowe P.S.N., de Zoysa P.A., Dong R., Wang H.R., White K.E., Econs M.J., Oudet C.L. Genomics 67:54-68(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone.
[2]	"Mepe, the gene encoding a tumor-secreted protein in oncogenic hypophosphatemic osteomalacia, is expressed in bone." Argiro L., Desbarats M., Glorieux F.H., Ecarot B. Genomics 74:342-351(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"A Human MEPE new exon between formerly identified exon 3 and exon 4." Wang X., Wang Y. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-101 (ISOFORM 2), ALTERNATIVE SPLICING.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"MEPE has the properties of an osteoblastic phosphatonin and minhibin." Rowe P.S., Kumagai Y., Gutierrez G., Garrett I.R., Blacher R., Rosen D., Cundy J., Navvab S., Chen D., Drezner M.K., Quarles L.D., Mundy G.R. Bone 34:303-319(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Secreted kinase phosphorylates extracellular proteins that regulate biomineralization." Tagliabracci V.S., Engel J.L., Wen J., Wiley S.E., Worby C.A., Kinch L.N., Xiao J., Grishin N.V., Dixon J.E. Science 336:1150-1153(2012) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY FAM20C.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ276396 mRNA. Translation: CAB97250.1. AF325916 mRNA. Translation: AAK70343.1. AC093768 Genomic DNA. No translation available. CH471057 Genomic DNA. Translation: EAX06001.1. CH471057 Genomic DNA. Translation: EAX06002.1. BC128158 mRNA. Translation: AAI28159.1. DQ854717 mRNA. Translation: ABI64294.1.
IPI	IPI00024648. IPI00791153.
RefSeq	NP_001171623.1. NM_001184694.1. NP_001171626.1. NM_001184697.1. NP_064588.1. NM_020203.3.
UniGene	Hs.676357.
3D structure databases
ProteinModelPortal	Q9NQ76.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NQ76. 8 interactions.
MINT	MINT-7241428.
STRING	9606.ENSP00000354341.
PTM databases
PhosphoSite	Q9NQ76.
Polymorphism databases
DMDM	33112396.
Proteomic databases
PaxDb	Q9NQ76.
PRIDE	Q9NQ76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361056; ENSP00000354341; ENSG00000152595. ENST00000395102; ENSP00000378534; ENSG00000152595. ENST00000424957; ENSP00000416984; ENSG00000152595.
GeneID	56955.
KEGG	hsa:56955.
UCSC	uc003hqy.3. human.
Organism-specific databases
CTD	56955.
GeneCards	GC04P088754.
HGNC	HGNC:13361. MEPE.
HPA	HPA038004.
MIM	605912. gene.
neXtProt	NX_Q9NQ76.
PharmGKB	PA30755.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43903.
HOGENOM	HOG000115910.
HOVERGEN	HBG004786.
OMA	QNAHQGK.
PhylomeDB	Q9NQ76.
Gene expression databases
ArrayExpress	Q9NQ76.
Bgee	Q9NQ76.
CleanEx	HS_MEPE.
Genevestigator	Q9NQ76.
GermOnline	ENSG00000152595. Homo sapiens.
Family and domain databases
InterPro	IPR009837. Osteoregulin. [Graphical view]
PANTHER	PTHR16510. PTHR16510. 1 hit.
Pfam	PF07175. Osteoregulin. 1 hit. [Graphical view]
ProDom	PD338624. Osteoregulin. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
GenomeRNAi	56955.
NextBio	62571.
SOURCE	Search...

Entry information

Entry name

MEPE_HUMAN

Accession

Primary (citable) accession number: Q9NQ76
Secondary accession number(s): A1A4X9, A8MTA3, D2CFR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.