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Q9NQ31 (AKIP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A-kinase-interacting protein 1
Alternative name(s):
Breast cancer-associated gene 3 protein
PKA-interacting protein
Proline-rich protein BCA3
Gene names
Name:AKIP1
Synonyms:BCA3, C11orf17
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Enhances NF-kappa-B transcriptional activity by regulating the nuclear localization of the NF-kappa-B subunit RELA and promoting the phosphorylation of RELA by PRKACA. Regulates the effect of the cAMP-dependent protein kinase signaling pathway on the NF-kappa-B activation cascade. Ref.6 Ref.7

Subunit structure

Interacts with PRKACA and RELA. Ref.5 Ref.6

Subcellular location

Nucleus. Note: Locates to punctate spots. Ref.5 Ref.6

Tissue specificity

Expressed at high levels in adult heart and at lower levels in brain, testis, ovary and skeletal muscle. Up-regulated in some breast cancer cell lines. Isoform 1 and isoform 3 are expressed in fetal brain. Ref.1 Ref.5

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DiseaseProto-oncogene
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsubstrate adhesion-dependent cell spreading

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRKACAP176124EBI-517035,EBI-476586

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NQ31-1)

Also known as: 1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NQ31-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-163: Missing.
Isoform 3 (identifier: Q9NQ31-3)

Also known as: 1b;

The sequence of this isoform differs from the canonical sequence as follows:
     75-101: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210A-kinase-interacting protein 1
PRO_0000064857

Natural variations

Alternative sequence75 – 10127Missing in isoform 3.
VSP_013275
Alternative sequence137 – 16327Missing in isoform 2.
VSP_013276
Natural variant231R → K. Ref.3 Ref.4
Corresponds to variant rs1133833 [ dbSNP | Ensembl ].
VAR_021565
Natural variant1321I → T.
Corresponds to variant rs35131475 [ dbSNP | Ensembl ].
VAR_050688

Experimental info

Sequence conflict411A → T in BAC11537. Ref.4
Sequence conflict921F → S in BAC11537. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (1a) [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: F00AA1F166C37967

FASTA21023,114
        10         20         30         40         50         60 
MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG VLAREAPHLE 

        70         80         90        100        110        120 
KQPAAGPQRV LPGEREERPP TLSASFRTMA EFMDYTSSQC GKYYSSVPEE GGATHVYRYH 

       130        140        150        160        170        180 
RGESKLHMCL DIGNGQRKDR KKTSLGPGGS YQISEHAPEA SQPAENISKD LYIEVYPGTY 

       190        200        210 
SVTVGSNDLT KKTHVVAVDS GQSVDLVFPV 

« Hide

Isoform 2 [UniParc].

Checksum: 872DD878B01C513B
Show »

FASTA18320,207
Isoform 3 (1b) [UniParc].

Checksum: CF22CF64A41F33DF
Show »

FASTA18320,034

References

« Hide 'large scale' references
[1]"Characterization of a novel human breast cancer associated gene (BCA3) encoding an alternatively spliced proline-rich protein."
Kitching R., Li H., Wong M.J., Kanaganayakam S., Kahn H., Seth A.
Biochim. Biophys. Acta 1625:116-121(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
Tissue: Lymph node.
[2]"Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7."
Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R.
Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-23.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-23.
Tissue: Kidney.
[5]"A-kinase-interacting protein localizes protein kinase A in the nucleus."
Sastri M., Barraclough D.M., Carmichael P.T., Taylor S.S.
Proc. Natl. Acad. Sci. U.S.A. 102:349-354(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PRKACA.
[6]"AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65."
Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T.
J. Biol. Chem. 283:7834-7843(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RELA, SUBCELLULAR LOCATION.
[7]"A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling."
Gao N., Hibi Y., Cueno M., Asamitsu K., Okamoto T.
J. Biol. Chem. 285:28097-28104(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF493783 mRNA. Translation: AAM12862.1.
AF493784 mRNA. Translation: AAM12863.1.
AF493785 mRNA. Translation: AAM12864.1.
AF493786 Genomic DNA. Translation: AAM12865.1.
AF493786 Genomic DNA. Translation: AAM12866.1.
AF493786 Genomic DNA. Translation: AAM12867.1.
AF512007 mRNA. Translation: AAM34785.1.
AJ400877 Genomic DNA. Translation: CAB92290.1.
AK075308 mRNA. Translation: BAC11537.1.
BC030996 mRNA. Translation: AAH30996.1.
RefSeqNP_001193575.1. NM_001206646.1.
NP_001193576.1. NM_001206647.1.
NP_001193577.1. NM_001206648.1.
NP_065693.2. NM_020642.3.
UniGeneHs.131180.

3D structure databases

ProteinModelPortalQ9NQ31.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121180. 9 interactions.
IntActQ9NQ31. 2 interactions.
STRING9606.ENSP00000310459.

PTM databases

PhosphoSiteQ9NQ31.

Proteomic databases

PaxDbQ9NQ31.
PRIDEQ9NQ31.

Protocols and materials databases

DNASU56672.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000299576; ENSP00000299576; ENSG00000166452. [Q9NQ31-3]
ENST00000309357; ENSP00000310644; ENSG00000166452. [Q9NQ31-2]
ENST00000309377; ENSP00000310459; ENSG00000166452. [Q9NQ31-1]
ENST00000396648; ENSP00000379885; ENSG00000166452. [Q9NQ31-3]
ENST00000534147; ENSP00000431331; ENSG00000166452. [Q9NQ31-1]
GeneID56672.
KEGGhsa:56672.
UCSCuc001mgx.3. human. [Q9NQ31-1]
uc001mgy.3. human. [Q9NQ31-3]
uc001mgz.3. human. [Q9NQ31-2]

Organism-specific databases

CTD56672.
GeneCardsGC11P008932.
H-InvDBHIX0009428.
HGNCHGNC:1170. AKIP1.
HPAHPA052455.
MIM609191. gene.
neXtProtNX_Q9NQ31.
PharmGKBPA25484.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40909.
HOVERGENHBG030457.
InParanoidQ9NQ31.
OMAHRGKSEL.
OrthoDBEOG712TZK.
PhylomeDBQ9NQ31.
TreeFamTF337318.

Gene expression databases

ArrayExpressQ9NQ31.
BgeeQ9NQ31.
CleanExHS_C11orf17.
GenevestigatorQ9NQ31.

Family and domain databases

ProtoNetSearch...

Other

ChiTaRSAKIP1. human.
GenomeRNAi56672.
NextBio62163.
PROQ9NQ31.
SOURCESearch...

Entry information

Entry nameAKIP1_HUMAN
AccessionPrimary (citable) accession number: Q9NQ31
Secondary accession number(s): Q8NBS2 expand/collapse secondary AC list , Q8TAC6, Q8TAD3, Q8TAE0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: October 23, 2007
Last modified: March 19, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM