Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

A-kinase-interacting protein 1

Gene

AKIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Enhances NF-kappa-B transcriptional activity by regulating the nuclear localization of the NF-kappa-B subunit RELA and promoting the phosphorylation of RELA by PRKACA. Regulates the effect of the cAMP-dependent protein kinase signaling pathway on the NF-kappa-B activation cascade.2 Publications

GO - Biological processi

  1. substrate adhesion-dependent cell spreading Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase-interacting protein 1
Alternative name(s):
Breast cancer-associated gene 3 protein
PKA-interacting protein
Proline-rich protein BCA3
Gene namesi
Name:AKIP1
Synonyms:BCA3, C11orf17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:1170. AKIP1.

Subcellular locationi

Nucleus 2 Publications
Note: Locates to punctate spots.

GO - Cellular componenti

  1. nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

PharmGKBiPA25484.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210A-kinase-interacting protein 1PRO_0000064857Add
BLAST

Proteomic databases

MaxQBiQ9NQ31.
PaxDbiQ9NQ31.
PRIDEiQ9NQ31.

PTM databases

PhosphoSiteiQ9NQ31.

Expressioni

Tissue specificityi

Expressed at high levels in adult heart and at lower levels in brain, testis, ovary and skeletal muscle. Up-regulated in some breast cancer cell lines. Isoform 1 and isoform 3 are expressed in fetal brain.2 Publications

Gene expression databases

BgeeiQ9NQ31.
CleanExiHS_C11orf17.
ExpressionAtlasiQ9NQ31. baseline and differential.
GenevestigatoriQ9NQ31.

Interactioni

Subunit structurei

Interacts with PRKACA and RELA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKACAP176124EBI-517035,EBI-476586

Protein-protein interaction databases

BioGridi121180. 8 interactions.
IntActiQ9NQ31. 2 interactions.
STRINGi9606.ENSP00000310459.

Structurei

3D structure databases

ProteinModelPortaliQ9NQ31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG40909.
GeneTreeiENSGT00390000017064.
HOVERGENiHBG030457.
InParanoidiQ9NQ31.
OMAiKRRAVNW.
OrthoDBiEOG712TZK.
PhylomeDBiQ9NQ31.
TreeFamiTF337318.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NQ31-1) [UniParc]FASTAAdd to basket

Also known as: 1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG
60 70 80 90 100
VLAREAPHLE KQPAAGPQRV LPGEREERPP TLSASFRTMA EFMDYTSSQC
110 120 130 140 150
GKYYSSVPEE GGATHVYRYH RGESKLHMCL DIGNGQRKDR KKTSLGPGGS
160 170 180 190 200
YQISEHAPEA SQPAENISKD LYIEVYPGTY SVTVGSNDLT KKTHVVAVDS
210
GQSVDLVFPV
Length:210
Mass (Da):23,114
Last modified:October 23, 2007 - v2
Checksum:iF00AA1F166C37967
GO
Isoform 2 (identifier: Q9NQ31-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-163: Missing.

Show »
Length:183
Mass (Da):20,207
Checksum:i872DD878B01C513B
GO
Isoform 3 (identifier: Q9NQ31-3) [UniParc]FASTAAdd to basket

Also known as: 1b

The sequence of this isoform differs from the canonical sequence as follows:
     75-101: Missing.

Show »
Length:183
Mass (Da):20,034
Checksum:iCF22CF64A41F33DF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411A → T in BAC11537 (PubMed:15489334).Curated
Sequence conflicti92 – 921F → S in BAC11537 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 231R → K.2 Publications
Corresponds to variant rs1133833 [ dbSNP | Ensembl ].
VAR_021565
Natural varianti132 – 1321I → T.
Corresponds to variant rs35131475 [ dbSNP | Ensembl ].
VAR_050688

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei75 – 10127Missing in isoform 3. 1 PublicationVSP_013275Add
BLAST
Alternative sequencei137 – 16327Missing in isoform 2. 1 PublicationVSP_013276Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF493783 mRNA. Translation: AAM12862.1.
AF493784 mRNA. Translation: AAM12863.1.
AF493785 mRNA. Translation: AAM12864.1.
AF493786 Genomic DNA. Translation: AAM12865.1.
AF493786 Genomic DNA. Translation: AAM12866.1.
AF493786 Genomic DNA. Translation: AAM12867.1.
AF512007 mRNA. Translation: AAM34785.1.
AJ400877 Genomic DNA. Translation: CAB92290.1.
AK075308 mRNA. Translation: BAC11537.1.
BC030996 mRNA. Translation: AAH30996.1.
CCDSiCCDS55743.1. [Q9NQ31-2]
CCDS55744.1. [Q9NQ31-3]
CCDS7793.1. [Q9NQ31-1]
RefSeqiNP_001193575.1. NM_001206646.1. [Q9NQ31-3]
NP_001193576.1. NM_001206647.1. [Q9NQ31-2]
NP_001193577.1. NM_001206648.1.
NP_065693.2. NM_020642.3. [Q9NQ31-1]
UniGeneiHs.131180.

Genome annotation databases

EnsembliENST00000299576; ENSP00000299576; ENSG00000166452. [Q9NQ31-3]
ENST00000309357; ENSP00000310644; ENSG00000166452. [Q9NQ31-2]
ENST00000309377; ENSP00000310459; ENSG00000166452. [Q9NQ31-1]
ENST00000396648; ENSP00000379885; ENSG00000166452. [Q9NQ31-3]
ENST00000534147; ENSP00000431331; ENSG00000166452. [Q9NQ31-1]
GeneIDi56672.
KEGGihsa:56672.
UCSCiuc001mgx.3. human. [Q9NQ31-1]
uc001mgy.3. human. [Q9NQ31-3]
uc001mgz.3. human. [Q9NQ31-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF493783 mRNA. Translation: AAM12862.1.
AF493784 mRNA. Translation: AAM12863.1.
AF493785 mRNA. Translation: AAM12864.1.
AF493786 Genomic DNA. Translation: AAM12865.1.
AF493786 Genomic DNA. Translation: AAM12866.1.
AF493786 Genomic DNA. Translation: AAM12867.1.
AF512007 mRNA. Translation: AAM34785.1.
AJ400877 Genomic DNA. Translation: CAB92290.1.
AK075308 mRNA. Translation: BAC11537.1.
BC030996 mRNA. Translation: AAH30996.1.
CCDSiCCDS55743.1. [Q9NQ31-2]
CCDS55744.1. [Q9NQ31-3]
CCDS7793.1. [Q9NQ31-1]
RefSeqiNP_001193575.1. NM_001206646.1. [Q9NQ31-3]
NP_001193576.1. NM_001206647.1. [Q9NQ31-2]
NP_001193577.1. NM_001206648.1.
NP_065693.2. NM_020642.3. [Q9NQ31-1]
UniGeneiHs.131180.

3D structure databases

ProteinModelPortaliQ9NQ31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121180. 8 interactions.
IntActiQ9NQ31. 2 interactions.
STRINGi9606.ENSP00000310459.

PTM databases

PhosphoSiteiQ9NQ31.

Proteomic databases

MaxQBiQ9NQ31.
PaxDbiQ9NQ31.
PRIDEiQ9NQ31.

Protocols and materials databases

DNASUi56672.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299576; ENSP00000299576; ENSG00000166452. [Q9NQ31-3]
ENST00000309357; ENSP00000310644; ENSG00000166452. [Q9NQ31-2]
ENST00000309377; ENSP00000310459; ENSG00000166452. [Q9NQ31-1]
ENST00000396648; ENSP00000379885; ENSG00000166452. [Q9NQ31-3]
ENST00000534147; ENSP00000431331; ENSG00000166452. [Q9NQ31-1]
GeneIDi56672.
KEGGihsa:56672.
UCSCiuc001mgx.3. human. [Q9NQ31-1]
uc001mgy.3. human. [Q9NQ31-3]
uc001mgz.3. human. [Q9NQ31-2]

Organism-specific databases

CTDi56672.
GeneCardsiGC11P008932.
H-InvDBHIX0009428.
HGNCiHGNC:1170. AKIP1.
MIMi609191. gene.
neXtProtiNX_Q9NQ31.
PharmGKBiPA25484.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG40909.
GeneTreeiENSGT00390000017064.
HOVERGENiHBG030457.
InParanoidiQ9NQ31.
OMAiKRRAVNW.
OrthoDBiEOG712TZK.
PhylomeDBiQ9NQ31.
TreeFamiTF337318.

Miscellaneous databases

ChiTaRSiAKIP1. human.
GenomeRNAii56672.
NextBioi62163.
PROiQ9NQ31.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NQ31.
CleanExiHS_C11orf17.
ExpressionAtlasiQ9NQ31. baseline and differential.
GenevestigatoriQ9NQ31.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel human breast cancer associated gene (BCA3) encoding an alternatively spliced proline-rich protein."
    Kitching R., Li H., Wong M.J., Kanaganayakam S., Kahn H., Seth A.
    Biochim. Biophys. Acta 1625:116-121(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
    Tissue: Lymph node.
  2. "Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7."
    Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R.
    Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-23.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-23.
    Tissue: Kidney.
  5. "A-kinase-interacting protein localizes protein kinase A in the nucleus."
    Sastri M., Barraclough D.M., Carmichael P.T., Taylor S.S.
    Proc. Natl. Acad. Sci. U.S.A. 102:349-354(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PRKACA.
  6. "AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65."
    Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T.
    J. Biol. Chem. 283:7834-7843(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RELA, SUBCELLULAR LOCATION.
  7. "A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling."
    Gao N., Hibi Y., Cueno M., Asamitsu K., Okamoto T.
    J. Biol. Chem. 285:28097-28104(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAKIP1_HUMAN
AccessioniPrimary (citable) accession number: Q9NQ31
Secondary accession number(s): Q8NBS2
, Q8TAC6, Q8TAD3, Q8TAE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: October 23, 2007
Last modified: March 4, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.