Q9NQ31 (AKIP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-kinase-interacting protein 1

Alternative name(s):
Breast cancer-associated gene 3 protein
PKA-interacting protein
Proline-rich protein BCA3

Gene names

Name:	AKIP1
Synonyms:	BCA3, C11orf17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enhances NF-kappa-B transcriptional activity by regulating the nuclear localization of the NF-kappa-B subunit RELA and promoting the phosphorylation of RELA by PRKACA. Regulates the effect of the cAMP-dependent protein kinase signaling pathway on the NF-kappa-B activation cascade. Ref.6 Ref.7
Subunit structure	Interacts with PRKACA and RELA. Ref.5 Ref.6
Subcellular location	Nucleus. Note: Locates to punctate spots. Ref.5 Ref.6
Tissue specificity	Expressed at high levels in adult heart and at lower levels in brain, testis, ovary and skeletal muscle. Up-regulated in some breast cancer cell lines. Isoform 1 and isoform 3 are expressed in fetal brain. Ref.1 Ref.5

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Proto-oncogene
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	substrate adhesion-dependent cell spreading Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRKACA	P17612	4	EBI-517035,EBI-476586

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQ31-1)

Also known as: 1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQ31-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-163: Missing.

Isoform 3 (identifier: Q9NQ31-3)

Also known as: 1b;

The sequence of this isoform differs from the canonical sequence as follows:
     75-101: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	A-kinase-interacting protein 1		PRO_0000064857
Natural variations
	Alternative sequence	75 – 101	27	Missing in isoform 3.		VSP_013275
	Alternative sequence	137 – 163	27	Missing in isoform 2.		VSP_013276
	Natural variant	23	1	R → K. Ref.3 Ref.4 Corresponds to variant rs1133833 [ dbSNP | Ensembl ].		VAR_021565
	Natural variant	132	1	I → T. Corresponds to variant rs35131475 [ dbSNP | Ensembl ].		VAR_050688
Experimental info
	Sequence conflict	41	1	A → T in BAC11537. Ref.4
	Sequence conflict	92	1	F → S in BAC11537. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (1a) [UniParc]. Last modified October 23, 2007. Version 2. Checksum: F00AA1F166C37967	FASTA	210	23,114
	10 20 30 40 50 60 MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG VLAREAPHLE 70 80 90 100 110 120 KQPAAGPQRV LPGEREERPP TLSASFRTMA EFMDYTSSQC GKYYSSVPEE GGATHVYRYH 130 140 150 160 170 180 RGESKLHMCL DIGNGQRKDR KKTSLGPGGS YQISEHAPEA SQPAENISKD LYIEVYPGTY 190 200 210 SVTVGSNDLT KKTHVVAVDS GQSVDLVFPV
	Isoform 2 [UniParc]. Checksum: 872DD878B01C513B	FASTA	183	20,207
	10 20 30 40 50 60 MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG VLAREAPHLE 70 80 90 100 110 120 KQPAAGPQRV LPGEREERPP TLSASFRTMA EFMDYTSSQC GKYYSSVPEE GGATHVYRYH 130 140 150 160 170 180 RGESKLHMCL DIGNGQAENI SKDLYIEVYP GTYSVTVGSN DLTKKTHVVA VDSGQSVDLV FPV
	Isoform 3 (1b) [UniParc]. Checksum: CF22CF64A41F33DF	FASTA	183	20,034
	10 20 30 40 50 60 MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG VLAREAPHLE 70 80 90 100 110 120 KQPAAGPQRV LPGEKYYSSV PEEGGATHVY RYHRGESKLH MCLDIGNGQR KDRKKTSLGP 130 140 150 160 170 180 GGSYQISEHA PEASQPAENI SKDLYIEVYP GTYSVTVGSN DLTKKTHVVA VDSGQSVDLV FPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel human breast cancer associated gene (BCA3) encoding an alternatively spliced proline-rich protein." Kitching R., Li H., Wong M.J., Kanaganayakam S., Kahn H., Seth A. Biochim. Biophys. Acta 1625:116-121(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY. Tissue: Lymph node.
[2]	"Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7." Amid C., Bahr A., Mujica A., Sampson N., Bikar S.E., Winterpacht A., Zabel B., Hankeln T., Schmidt E.R. Cytogenet. Cell Genet. 93:284-290(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-23.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-23. Tissue: Kidney.
[5]	"A-kinase-interacting protein localizes protein kinase A in the nucleus." Sastri M., Barraclough D.M., Carmichael P.T., Taylor S.S. Proc. Natl. Acad. Sci. U.S.A. 102:349-354(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PRKACA.
[6]	"AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65." Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T. J. Biol. Chem. 283:7834-7843(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RELA, SUBCELLULAR LOCATION.
[7]	"A-kinase-interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling." Gao N., Hibi Y., Cueno M., Asamitsu K., Okamoto T. J. Biol. Chem. 285:28097-28104(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF493783 mRNA. Translation: AAM12862.1. AF493784 mRNA. Translation: AAM12863.1. AF493785 mRNA. Translation: AAM12864.1. AF493786 Genomic DNA. Translation: AAM12865.1. AF493786 Genomic DNA. Translation: AAM12866.1. AF493786 Genomic DNA. Translation: AAM12867.1. AF512007 mRNA. Translation: AAM34785.1. AJ400877 Genomic DNA. Translation: CAB92290.1. AK075308 mRNA. Translation: BAC11537.1. BC030996 mRNA. Translation: AAH30996.1.
IPI	IPI00152039. IPI00152045. IPI00299443.
RefSeq	NP_001193575.1. NM_001206646.1. NP_001193576.1. NM_001206647.1. NP_001193577.1. NM_001206648.1. NP_065693.2. NM_020642.3.
UniGene	Hs.131180.
3D structure databases
ProteinModelPortal	Q9NQ31.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NQ31. 2 interactions.
STRING	9606.ENSP00000310459.
PTM databases
PhosphoSite	Q9NQ31.
Proteomic databases
PaxDb	Q9NQ31.
PRIDE	Q9NQ31.
Protocols and materials databases
DNASU	56672.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299576; ENSP00000299576; ENSG00000166452. ENST00000309357; ENSP00000310644; ENSG00000166452. ENST00000309377; ENSP00000310459; ENSG00000166452. ENST00000396648; ENSP00000379885; ENSG00000166452. ENST00000534147; ENSP00000431331; ENSG00000166452.
GeneID	56672.
KEGG	hsa:56672.
UCSC	uc001mgx.3. human. uc001mgy.3. human. uc001mgz.3. human.
Organism-specific databases
CTD	56672.
GeneCards	GC11P008932.
H-InvDB	HIX0009428.
HGNC	HGNC:1170. AKIP1.
MIM	609191. gene.
neXtProt	NX_Q9NQ31.
PharmGKB	PA25484.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG40909.
HOVERGEN	HBG030457.
InParanoid	Q9NQ31.
OMA	HRGKSEL.
OrthoDB	EOG4XWFZX.
PhylomeDB	Q9NQ31.
Gene expression databases
ArrayExpress	Q9NQ31.
Bgee	Q9NQ31.
CleanEx	HS_C11orf17.
Genevestigator	Q9NQ31.
GermOnline	ENSG00000166452. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	AKIP1. human.
GenomeRNAi	56672.
NextBio	62163.
SOURCE	Search...

Entry information

Entry name

AKIP1_HUMAN

Accession

Primary (citable) accession number: Q9NQ31
Secondary accession number(s): Q8NBS2 , Q8TAC6, Q8TAD3, Q8TAE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	October 23, 2007
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.