Q9NQ25 (SLAF7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SLAM family member 7

Alternative name(s):
CD2 subset 1
CD2-like receptor-activating cytotoxic cells
Short name=CRACC
Membrane protein FOAP-12
Novel Ly9
Protein 19A
CD_antigen=CD319

Gene names

Name:	SLAMF7
Synonyms:	CS1
ORF Names:	UNQ576/PRO1138

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Ref.2 Ref.3 Isoform 3 does not mediate any NK cell activation. Ref.2 Ref.3
Subunit structure	Isoform 1 binds to SH2D1A when its cytoplasmic tail is phosphorylated in the presence of FYN (in vitro).
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Isoform 3 is expressed at much lower level than isoform 1. Ref.1 Ref.2 Ref.3 Ref.11
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Sequence caution	The sequence CAB76561.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Non-traceable author statement Ref.3. Source: UniProtKB natural killer cell activation Non-traceable author statement Ref.2. Source: UniProtKB natural killer cell mediated cytotoxicity Non-traceable author statement Ref.2. Source: UniProtKB regulation of natural killer cell activation Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NQ25-1)

Also known as: 19A; CS1-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NQ25-2)

The sequence of this isoform differs from the canonical sequence as follows:
     19-125: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NQ25-3)

Also known as: 19A24; CS1-S;

The sequence of this isoform differs from the canonical sequence as follows:
     258-296: YIEEKKRVDI...IPHTNRTILK → NNPKGRSSKY...DARHTKAICL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.9
	Chain	23 – 335	313	SLAM family member 7		PRO_0000014963
Regions
	Topological domain	23 – 226	204	Extracellular Potential
	Transmembrane	227 – 247	21	Helical; Potential
	Topological domain	248 – 335	88	Cytoplasmic Potential
	Domain	131 – 206	76	Ig-like C2-type
Amino acid modifications
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Disulfide bond	145 ↔ 215		Potential
	Disulfide bond	151 ↔ 195		By similarity
Natural variations
	Alternative sequence	19 – 125	107	Missing in isoform 2.		VSP_013781
	Alternative sequence	258 – 296	39	YIEEK…RTILK → NNPKGRSSKYGLLHCGNTEK DGKSPLTAHDARHTKAICL in isoform 3.		VSP_013782
	Natural variant	175	1	H → Y. Corresponds to variant rs35325048 [ dbSNP | Ensembl ].		VAR_049938
	Natural variant	302	1	T → M. Corresponds to variant rs2295617 [ dbSNP | Ensembl ].		VAR_049939
Experimental info
	Sequence conflict	135	1	M → L in CAB81950. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (19A) (CS1-L) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D09ABBCFF74BE8D4	FASTA	335	37,421
	10 20 30 40 50 60 MAGSPTCLTL IYILWQLTGS AASGPVKELV GSVGGAVTFP LKSKVKQVDS IVWTFNTTPL 70 80 90 100 110 120 VTIQPEGGTI IVTQNRNRER VDFPDGGYSL KLSKLKKNDS GIYYVGIYSS SLQQPSTQEY 130 140 150 160 170 180 VLHVYEHLSK PKVTMGLQSN KNGTCVTNLT CCMEHGEEDV IYTWKALGQA ANESHNGSIL 190 200 210 220 230 240 PISWRWGESD MTFICVARNP VSRNFSSPIL ARKLCEGAAD DPDSSMVLLC LLLVPLLLSL 250 260 270 280 290 300 FVLGLFLWFL KRERQEEYIE EKKRVDICRE TPNICPHSGE NTEYDTIPHT NRTILKEDPA 310 320 330 NTVYSTVEIP KKMENPHSLL TMPDTPRLFA YENVI
	Isoform 2 [UniParc]. Checksum: 2B01DB70E7BBFC14	FASTA	228	25,831
	10 20 30 40 50 60 MAGSPTCLTL IYILWQLTEH LSKPKVTMGL QSNKNGTCVT NLTCCMEHGE EDVIYTWKAL 70 80 90 100 110 120 GQAANESHNG SILPISWRWG ESDMTFICVA RNPVSRNFSS PILARKLCEG AADDPDSSMV 130 140 150 160 170 180 LLCLLLVPLL LSLFVLGLFL WFLKRERQEE YIEEKKRVDI CRETPNICPH SGENTEYDTI 190 200 210 220 PHTNRTILKE DPANTVYSTV EIPKKMENPH SLLTMPDTPR LFAYENVI
	Isoform 3 (19A24) (CS1-S) [UniParc]. Checksum: 3CD49C30FF3367B0	FASTA	335	37,026
	10 20 30 40 50 60 MAGSPTCLTL IYILWQLTGS AASGPVKELV GSVGGAVTFP LKSKVKQVDS IVWTFNTTPL 70 80 90 100 110 120 VTIQPEGGTI IVTQNRNRER VDFPDGGYSL KLSKLKKNDS GIYYVGIYSS SLQQPSTQEY 130 140 150 160 170 180 VLHVYEHLSK PKVTMGLQSN KNGTCVTNLT CCMEHGEEDV IYTWKALGQA ANESHNGSIL 190 200 210 220 230 240 PISWRWGESD MTFICVARNP VSRNFSSPIL ARKLCEGAAD DPDSSMVLLC LLLVPLLLSL 250 260 270 280 290 300 FVLGLFLWFL KRERQEENNP KGRSSKYGLL HCGNTEKDGK SPLTAHDARH TKAICLEDPA 310 320 330 NTVYSTVEIP KKMENPHSLL TMPDTPRLFA YENVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of CS1, a novel human natural killer cell receptor belonging to the CD2 subset of the immunoglobulin superfamily." Boles K.S., Mathew P.A. Immunogenetics 52:302-307(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family." Bouchon A., Cella M., Grierson H.L., Cohen J.I., Colonna M. J. Immunol. 167:5517-5521(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[3]	"A novel immunoglobulin superfamily receptor (19A) related to CD2 is expressed on activated lymphocytes and promotes homotypic B-cell adhesion." Murphy J.J., Hobby P., Vilarino-Varela J., Bishop B., Iordanidou P., Sutton B.J., Norton J.D. Biochem. J. 361:431-436(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), FUNCTION, TISSUE SPECIFICITY.
[4]	"Homo sapiens mRNA for FOAP-12 protein, complete cds." Fujii Y., Takayama K., Tsuritani K., Yajima Y., Amemiya T., Ukai Y., Naito K., Kawaguchi A. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Macrophage.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lymph node.
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Fetal lung and Fetal spleen.
[9]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-37.
[10]	"Molecular and functional characterization of a CS1 (CRACC) splice variant expressed in human NK cells that does not contain immunoreceptor tyrosine-based switch motifs." Lee J.K., Boles K.S., Mathew P.A. Eur. J. Immunol. 34:2791-2799(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORM 3).
[11]	"Mouse novel Ly9: a new member of the expanding CD150 (SLAM) family of leukocyte cell-surface receptors." Tovar V., Del Valle J., Zapater N., Martin M., Romero X., Pizcueta P., Bosch J., Terhorst C., Engel P. Immunogenetics 54:394-402(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH SH2D1A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF291815 mRNA. Translation: AAK11549.1. AF390894 mRNA. Translation: AAL26989.1. AJ271869 mRNA. Translation: CAB76561.1. Frameshift. AJ276429 mRNA. Translation: CAB81950.2. AB027233 mRNA. Translation: BAB61022.1. AY358512 mRNA. Translation: AAQ88876.1. AL834424 mRNA. Translation: CAD39085.1. AL121985 Genomic DNA. Translation: CAC00579.1. AL121985 Genomic DNA. Translation: CAH73507.1. AL121985 Genomic DNA. Translation: CAH73508.1. BC027867 mRNA. Translation: AAH27867.1.
IPI	IPI00099863. IPI00167137. IPI00168447.
RefSeq	NP_067004.3. NM_021181.3.
UniGene	Hs.517265.
3D structure databases
ProteinModelPortal	Q9NQ25.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NQ25. 3 interactions.
STRING	9606.ENSP00000357022.
PTM databases
PhosphoSite	Q9NQ25.
Polymorphism databases
DMDM	66774034.
Proteomic databases
PaxDb	Q9NQ25.
PRIDE	Q9NQ25.
Protocols and materials databases
DNASU	57823.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000368042; ENSP00000357021; ENSG00000026751. ENST00000368043; ENSP00000357022; ENSG00000026751.
GeneID	57823.
KEGG	hsa:57823.
UCSC	uc001fwq.3. human. uc001fws.3. human.
Organism-specific databases
CTD	57823.
GeneCards	GC01P160709.
HGNC	HGNC:21394. SLAMF7.
HPA	CAB015445.
MIM	606625. gene.
neXtProt	NX_Q9NQ25.
PharmGKB	PA134977110.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG272174.
HOGENOM	HOG000013065.
HOVERGEN	HBG079189.
InParanoid	Q9NQ25.
KO	K06733.
OMA	EDVTYSW.
OrthoDB	EOG4CRM0K.
PhylomeDB	Q9NQ25.
Gene expression databases
ArrayExpress	Q9NQ25.
Bgee	Q9NQ25.
CleanEx	HS_SLAMF7.
Genevestigator	Q9NQ25.
GermOnline	ENSG00000026751. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. IPR015631. SLAM_fam_rcpts. [Graphical view]
PANTHER	PTHR12080. PTHR12080. 1 hit.
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57823.
NextBio	64798.
SOURCE	Search...

Entry information

Entry name

SLAF7_HUMAN

Accession

Primary (citable) accession number: Q9NQ25
Secondary accession number(s): Q8N6Y8 , Q8ND32, Q9NY08, Q9NY23

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.