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Q9NPZ5

- B3GA2_HUMAN

UniProt

Q9NPZ5 - B3GA2_HUMAN

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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2

Gene
B3GAT2, GLCATS, KIAA1963
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins By similarity.

Catalytic activityi

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Cofactori

Manganese.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181UDP-glucuronate By similarity
Binding sitei155 – 1551UDP-glucuronate By similarity
Binding sitei160 – 1601UDP-glucuronate By similarity
Metal bindingi187 – 1871Manganese By similarity
Sitei218 – 2181Interaction with galactose moiety of substrate glycoprotein By similarity
Active sitei273 – 2731Proton donor/acceptor By similarity
Sitei310 – 3101Interaction with galactose moiety of substrate glycoprotein By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 893UDP-glucuronate binding By similarity
Nucleotide bindingi185 – 1873UDP-glucuronate binding By similarity
Nucleotide bindingi300 – 3023UDP-glucuronate binding By similarity

GO - Molecular functioni

  1. galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. chondroitin sulfate metabolic process Source: Reactome
  3. glycosaminoglycan metabolic process Source: Reactome
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS03557-MONOMER.
ReactomeiREACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (EC:2.4.1.135)
Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name:
GlcAT-S
Short name:
Glucuronosyltransferase S
Gene namesi
Name:B3GAT2
Synonyms:GLCATS, KIAA1963
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:922. B3GAT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22Cytoplasmic Reviewed prediction
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini24 – 323300Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25216.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2PRO_0000195172Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi67 – 671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi292 – 2921N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NPZ5.
PRIDEiQ9NPZ5.

Expressioni

Tissue specificityi

Expressed in the trachea, retina, spinal cord, hippocampus and other brain regions, and, at lower levels, in testis and ovary.

Gene expression databases

BgeeiQ9NPZ5.
CleanExiHS_B3GAT2.
GenevestigatoriQ9NPZ5.

Organism-specific databases

HPAiHPA012059.

Interactioni

Subunit structurei

Homodimer Reviewed prediction.

Protein-protein interaction databases

STRINGi9606.ENSP00000230053.

Structurei

Secondary structure

1
323
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi82 – 887
Helixi94 – 10512
Beta strandi111 – 12111
Helixi124 – 1329
Beta strandi137 – 1415
Helixi157 – 17014
Beta strandi173 – 1753
Beta strandi180 – 1834
Helixi193 – 1986
Beta strandi202 – 2065
Beta strandi209 – 2124
Beta strandi215 – 2239
Beta strandi226 – 2316
Helixi244 – 2463
Beta strandi247 – 2504
Helixi251 – 2566
Beta strandi266 – 2683
Helixi269 – 2713
Helixi272 – 2787
Helixi283 – 2853
Helixi290 – 2934
Beta strandi313 – 3153

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D0JX-ray2.00A/B/C/D78-323[»]
ProteinModelPortaliQ9NPZ5.
SMRiQ9NPZ5. Positions 78-323.

Miscellaneous databases

EvolutionaryTraceiQ9NPZ5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni234 – 24310Interaction with galactose moiety of substrate glycoprotein By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292627.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiQ9NPZ5.
KOiK10157.
OMAiFILLPWV.
OrthoDBiEOG7QG44X.
PhylomeDBiQ9NPZ5.
TreeFamiTF313522.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NPZ5-1 [UniParc]FASTAAdd to Basket

« Hide

MKSALFTRFF ILLPWILIVI IMLDVDTRRP VPPLTPRPYF SPYAVGRGGA    50
RLPLRRGGPA HGTQKRNQSR PQPQPEPQLP TIYAITPTYS RPVQKAELTR 100
LANTFRQVAQ LHWILVEDAA ARSELVSRFL ARAGLPSTHL HVPTPRRYKR 150
PGLPRATEQR NAGLAWLRQR HQHQRAQPGV LFFADDDNTY SLELFQEMRT 200
TRKVSVWPVG LVGGRRYERP LVENGKVVGW YTGWRADRPF AIDMAGFAVS 250
LQVILSNPKA VFKRRGSQPG MQESDFLKQI TTVEELEPKA NNCTKVLVWH 300
TRTEKVNLAN EPKYHLDTVK IEV 323
Length:323
Mass (Da):36,919
Last modified:June 1, 2001 - v2
Checksum:i85058D52D2D28463
GO

Sequence cautioni

The sequence BAB70889.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB85549.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY070019 mRNA. Translation: AAL57718.1.
AY070110, AY070108, AY070109 Genomic DNA. Translation: AAL58977.1.
AB075843 mRNA. Translation: BAB85549.1. Different initiation.
AL121961, AL450320 Genomic DNA. Translation: CAI42145.1.
AL450320, AL121961 Genomic DNA. Translation: CAI39582.1.
AK055248 mRNA. Translation: BAB70889.1. Different initiation.
CCDSiCCDS4974.1.
RefSeqiNP_542780.1. NM_080742.2.
UniGeneiHs.713609.

Genome annotation databases

EnsembliENST00000230053; ENSP00000230053; ENSG00000112309.
GeneIDi135152.
KEGGihsa:135152.
UCSCiuc003pfv.3. human.

Polymorphism databases

DMDMi14285363.

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY070019 mRNA. Translation: AAL57718.1 .
AY070110 , AY070108 , AY070109 Genomic DNA. Translation: AAL58977.1 .
AB075843 mRNA. Translation: BAB85549.1 . Different initiation.
AL121961 , AL450320 Genomic DNA. Translation: CAI42145.1 .
AL450320 , AL121961 Genomic DNA. Translation: CAI39582.1 .
AK055248 mRNA. Translation: BAB70889.1 . Different initiation.
CCDSi CCDS4974.1.
RefSeqi NP_542780.1. NM_080742.2.
UniGenei Hs.713609.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2D0J X-ray 2.00 A/B/C/D 78-323 [» ]
ProteinModelPortali Q9NPZ5.
SMRi Q9NPZ5. Positions 78-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000230053.

Protein family/group databases

CAZyi GT43. Glycosyltransferase Family 43.

Polymorphism databases

DMDMi 14285363.

Proteomic databases

PaxDbi Q9NPZ5.
PRIDEi Q9NPZ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000230053 ; ENSP00000230053 ; ENSG00000112309 .
GeneIDi 135152.
KEGGi hsa:135152.
UCSCi uc003pfv.3. human.

Organism-specific databases

CTDi 135152.
GeneCardsi GC06M071566.
HGNCi HGNC:922. B3GAT2.
HPAi HPA012059.
MIMi 607497. gene.
neXtProti NX_Q9NPZ5.
PharmGKBi PA25216.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG292627.
HOGENOMi HOG000261693.
HOVERGENi HBG050650.
InParanoidi Q9NPZ5.
KOi K10157.
OMAi FILLPWV.
OrthoDBi EOG7QG44X.
PhylomeDBi Q9NPZ5.
TreeFami TF313522.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci MetaCyc:HS03557-MONOMER.
Reactomei REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

EvolutionaryTracei Q9NPZ5.
GeneWikii B3GAT2.
GenomeRNAii 135152.
NextBioi 83461.
PROi Q9NPZ5.
SOURCEi Search...

Gene expression databases

Bgeei Q9NPZ5.
CleanExi HS_B3GAT2.
Genevestigatori Q9NPZ5.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10896. PTHR10896. 1 hit.
Pfami PF03360. Glyco_transf_43. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, characterization, and chromosome mapping of the human GlcAT-S gene."
    Marcos I., Galan J.J., Borrego S., Antinolo G.
    J. Hum. Genet. 47:677-680(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
    Nagase T., Kikuno R., Ohara O.
    DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-323.
    Tissue: Fetal brain.
  5. "Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope."
    Shiba T., Kakuda S., Ishiguro M., Morita I., Oka S., Kawasaki T., Wakatsuki S., Kato R.
    Proteins 65:499-508(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 78-323.

Entry informationi

Entry nameiB3GA2_HUMAN
AccessioniPrimary (citable) accession number: Q9NPZ5
Secondary accession number(s): Q5JS09, Q8TF38, Q96NK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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