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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2

Gene

B3GAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins.By similarity

Catalytic activityi

UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.By similarity

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118UDP-glucuronateBy similarity1
Binding sitei155UDP-glucuronateBy similarity1
Binding sitei160UDP-glucuronateBy similarity1
Metal bindingi187ManganeseBy similarity1
Active sitei273Proton donor/acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi87 – 89UDP-glucuronate bindingBy similarity3
Nucleotide bindingi185 – 187UDP-glucuronate bindingBy similarity3
Nucleotide bindingi300 – 302UDP-glucuronate bindingBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS03557-MONOMER.
ZFISH:HS03557-MONOMER.
ReactomeiR-HSA-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (EC:2.4.1.135By similarity)
Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name:
GlcAT-S
Short name:
Glucuronosyltransferase S
Gene namesi
Name:B3GAT2
Synonyms:GLCATS, KIAA1963
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:922. B3GAT2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2CytoplasmicSequence analysis2
Transmembranei3 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini24 – 323LumenalSequence analysisAdd BLAST300

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi135152.
OpenTargetsiENSG00000112309.
PharmGKBiPA25216.

Polymorphism and mutation databases

BioMutaiB3GAT2.
DMDMi14285363.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951721 – 323Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2Add BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi67N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9NPZ5.
PaxDbiQ9NPZ5.
PeptideAtlasiQ9NPZ5.
PRIDEiQ9NPZ5.

PTM databases

iPTMnetiQ9NPZ5.
PhosphoSitePlusiQ9NPZ5.

Expressioni

Tissue specificityi

Expressed in the trachea, retina, spinal cord, hippocampus and other brain regions, and, at lower levels, in testis and ovary.

Gene expression databases

BgeeiENSG00000112309.
CleanExiHS_B3GAT2.
ExpressionAtlasiQ9NPZ5. baseline and differential.
GenevisibleiQ9NPZ5. HS.

Organism-specific databases

HPAiHPA012059.

Interactioni

Subunit structurei

Homodimer.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei218Interaction with galactose moiety of substrate glycoproteinBy similarity1
Sitei310Interaction with galactose moiety of substrate glycoproteinBy similarity1

Protein-protein interaction databases

STRINGi9606.ENSP00000230053.

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi82 – 88Combined sources7
Helixi94 – 105Combined sources12
Beta strandi111 – 121Combined sources11
Helixi124 – 132Combined sources9
Beta strandi137 – 141Combined sources5
Helixi157 – 170Combined sources14
Beta strandi173 – 175Combined sources3
Beta strandi180 – 183Combined sources4
Helixi193 – 198Combined sources6
Beta strandi202 – 206Combined sources5
Beta strandi209 – 212Combined sources4
Beta strandi215 – 223Combined sources9
Beta strandi226 – 231Combined sources6
Helixi244 – 246Combined sources3
Beta strandi247 – 250Combined sources4
Helixi251 – 256Combined sources6
Beta strandi266 – 268Combined sources3
Helixi269 – 271Combined sources3
Helixi272 – 278Combined sources7
Helixi283 – 285Combined sources3
Helixi290 – 293Combined sources4
Beta strandi313 – 315Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D0JX-ray2.00A/B/C/D78-323[»]
ProteinModelPortaliQ9NPZ5.
SMRiQ9NPZ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NPZ5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni234 – 243Interaction with galactose moiety of substrate glycoproteinBy similarity10

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiQ9NPZ5.
KOiK10157.
OMAiEEMRTTH.
OrthoDBiEOG091G0G8P.
PhylomeDBiQ9NPZ5.
TreeFamiTF313522.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NPZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSALFTRFF ILLPWILIVI IMLDVDTRRP VPPLTPRPYF SPYAVGRGGA
60 70 80 90 100
RLPLRRGGPA HGTQKRNQSR PQPQPEPQLP TIYAITPTYS RPVQKAELTR
110 120 130 140 150
LANTFRQVAQ LHWILVEDAA ARSELVSRFL ARAGLPSTHL HVPTPRRYKR
160 170 180 190 200
PGLPRATEQR NAGLAWLRQR HQHQRAQPGV LFFADDDNTY SLELFQEMRT
210 220 230 240 250
TRKVSVWPVG LVGGRRYERP LVENGKVVGW YTGWRADRPF AIDMAGFAVS
260 270 280 290 300
LQVILSNPKA VFKRRGSQPG MQESDFLKQI TTVEELEPKA NNCTKVLVWH
310 320
TRTEKVNLAN EPKYHLDTVK IEV
Length:323
Mass (Da):36,919
Last modified:June 1, 2001 - v2
Checksum:i85058D52D2D28463
GO

Sequence cautioni

The sequence BAB70889 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB85549 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY070019 mRNA. Translation: AAL57718.1.
AY070110, AY070108, AY070109 Genomic DNA. Translation: AAL58977.1.
AB075843 mRNA. Translation: BAB85549.1. Different initiation.
AL121961, AL450320 Genomic DNA. Translation: CAI42145.1.
AL450320, AL121961 Genomic DNA. Translation: CAI39582.1.
AK055248 mRNA. Translation: BAB70889.1. Different initiation.
CCDSiCCDS4974.1.
RefSeqiNP_542780.1. NM_080742.2.
UniGeneiHs.713609.

Genome annotation databases

EnsembliENST00000230053; ENSP00000230053; ENSG00000112309.
GeneIDi135152.
KEGGihsa:135152.
UCSCiuc003pfv.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY070019 mRNA. Translation: AAL57718.1.
AY070110, AY070108, AY070109 Genomic DNA. Translation: AAL58977.1.
AB075843 mRNA. Translation: BAB85549.1. Different initiation.
AL121961, AL450320 Genomic DNA. Translation: CAI42145.1.
AL450320, AL121961 Genomic DNA. Translation: CAI39582.1.
AK055248 mRNA. Translation: BAB70889.1. Different initiation.
CCDSiCCDS4974.1.
RefSeqiNP_542780.1. NM_080742.2.
UniGeneiHs.713609.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D0JX-ray2.00A/B/C/D78-323[»]
ProteinModelPortaliQ9NPZ5.
SMRiQ9NPZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000230053.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

iPTMnetiQ9NPZ5.
PhosphoSitePlusiQ9NPZ5.

Polymorphism and mutation databases

BioMutaiB3GAT2.
DMDMi14285363.

Proteomic databases

MaxQBiQ9NPZ5.
PaxDbiQ9NPZ5.
PeptideAtlasiQ9NPZ5.
PRIDEiQ9NPZ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230053; ENSP00000230053; ENSG00000112309.
GeneIDi135152.
KEGGihsa:135152.
UCSCiuc003pfv.4. human.

Organism-specific databases

CTDi135152.
DisGeNETi135152.
GeneCardsiB3GAT2.
HGNCiHGNC:922. B3GAT2.
HPAiHPA012059.
MIMi607497. gene.
neXtProtiNX_Q9NPZ5.
OpenTargetsiENSG00000112309.
PharmGKBiPA25216.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiQ9NPZ5.
KOiK10157.
OMAiEEMRTTH.
OrthoDBiEOG091G0G8P.
PhylomeDBiQ9NPZ5.
TreeFamiTF313522.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:HS03557-MONOMER.
ZFISH:HS03557-MONOMER.
ReactomeiR-HSA-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

ChiTaRSiB3GAT2. human.
EvolutionaryTraceiQ9NPZ5.
GeneWikiiB3GAT2.
GenomeRNAii135152.
PROiQ9NPZ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112309.
CleanExiHS_B3GAT2.
ExpressionAtlasiQ9NPZ5. baseline and differential.
GenevisibleiQ9NPZ5. HS.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3GA2_HUMAN
AccessioniPrimary (citable) accession number: Q9NPZ5
Secondary accession number(s): Q5JS09, Q8TF38, Q96NK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.