Reviewed,
UniProtKB/Swiss-Prot Q9NPJ3 (ACO13_HUMAN)
Last modified
December 15, 2009.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Acyl-coenzyme A thioesterase 13 Short name=Acyl-CoA thioesterase 13 EC=3.1.2.- Alternative name(s): Thioesterase superfamily member 2 | ||||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 140 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Ref.7 Ref.8 |
| Subunit structure | |
| Subcellular location | Cytoplasm By similarity. Mitochondrion By similarity. |
| Sequence similarities | Belongs to the thioesterase paaI family. |
| Biophysicochemical properties | Kinetic parameters: KM=4.9 µM for n-decanoyl-CoA KM=26 µM for n-octanoyl-CoA KM=5 µM for myristoyl-CoA KM=9 µM for palmitoyl-CoA KM=9 µM for oleoyl-CoA KM=10 µM for 3,4-dihydrohyphenylacetyl-CoA KM=40 µM for 3-hydrohyphenylacetyl-CoA |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Mitochondrion |
| Molecular function | Hydrolase |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | protein homotetramerization Ref.7 Ref.8 Inferred from physical interaction. Source: UniProtKB |
| Cellular component | mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | acyl-CoA thioesterase activity Ref.7 Ref.8 Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 140 | 140 | Acyl-coenzyme A thioesterase 13 | PRO_0000156697 | ||||||||||||||||||||||||||||||
Sites | ||||||||||||||||||||||||||||||||||
| Binding site | 50 | 1 | Substrate | |||||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||||
| Mutagenesis | 50 | 1 | N → A: Reduced activity. Ref.8 | |||||||||||||||||||||||||||||||
| Mutagenesis | 56 | 1 | H → A: Decreases affinity for substrate. Ref.8 | |||||||||||||||||||||||||||||||
| Mutagenesis | 65 | 1 | D → A: Loss of activity. Ref.7 Ref.8 | |||||||||||||||||||||||||||||||
| Mutagenesis | 65 | 1 | D → E or N: Reduced activity. Ref.7 Ref.8 | |||||||||||||||||||||||||||||||
| Mutagenesis | 83 | 1 | S → A: Reduced activity. Ref.8 | |||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||
| Helix | 4 – 17 | 14 | ||||||||||||||||||||||||||||||||
| Helix | 21 – 25 | 5 | ||||||||||||||||||||||||||||||||
| Beta strand | 29 – 34 | 6 | ||||||||||||||||||||||||||||||||
| Beta strand | 37 – 43 | 7 | ||||||||||||||||||||||||||||||||
| Helix | 46 – 48 | 3 | ||||||||||||||||||||||||||||||||
| Beta strand | 53 – 55 | 3 | ||||||||||||||||||||||||||||||||
| Helix | 57 – 73 | 17 | ||||||||||||||||||||||||||||||||
| Beta strand | 75 – 77 | 3 | ||||||||||||||||||||||||||||||||
| Beta strand | 82 – 90 | 9 | ||||||||||||||||||||||||||||||||
| Beta strand | 99 – 109 | 11 | ||||||||||||||||||||||||||||||||
| Beta strand | 111 – 122 | 12 | ||||||||||||||||||||||||||||||||
| Turn | 123 – 125 | 3 | ||||||||||||||||||||||||||||||||
| Beta strand | 128 – 137 | 10 | ||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. Chen J.-L.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland and Hypothalamus. |
| [2] | "Human acute promyelocytic leukemia cell line NB4's apoptosis related genes." Yu W.-Q., Sun B.-Z., Chai Y.-B., Zhu F., Liu X.-S., Li Z., Lu F., Yan W., Yang H., Zhao Z.-L. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Promyelocytic leukemia. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix. |
| [6] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [7] | "Crystal structure of human thioesterase superfamily member 2." Cheng Z., Song F., Shan X., Wei Z., Wang Y., Dunaway-Mariano D., Gong W. Biochem. Biophys. Res. Commun. 349:172-177(2006) [PubMed: 16934754] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, MASS SPECTROMETRY, MUTAGENESIS OF ASP-65, SUBUNIT. |
| [8] | "The mechanisms of human hotdog-fold thioesterase 2 (hTHEM2) substrate recognition and catalysis illuminated by a structure and function based analysis." Cao J., Xu H., Zhao H., Gong W., Dunaway-Mariano D. Biochemistry 48:1293-1304(2009) [PubMed: 19170545] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ASN-50; HIS-56; ASP-65 AND SER-83, SUBUNIT. |
| [9] | "The crystal structure of human thioesterase superfamily member 2." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AF155649 mRNA. Translation: AAF67006.1. AF220186 mRNA. Translation: AAF67651.1. AF274952 mRNA. Translation: AAK07529.1. AK000508 mRNA. Translation: BAA91215.1. AL031775 Genomic DNA. Translation: CAD92509.1. BC000894 mRNA. Translation: AAH00894.1. | |||||||||||||||||||||||||
| IPI | IPI00020530. | ||||||||||||||||||||||||
| RefSeq | NP_060943.1. | ||||||||||||||||||||||||
| UniGene | Hs.519930 Hs.9676 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | Q9NPJ3. 3 interactions. | ||||||||||||||||||||||||
| STRING | Q9NPJ3. | ||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||
| PeptideAtlas | Q9NPJ3. | ||||||||||||||||||||||||
| PRIDE | Q9NPJ3. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENST00000230048; ENSP00000230048; ENSG00000112304; Homo sapiens. [Genome view] | ||||||||||||||||||||||||
| GeneID | 55856. | ||||||||||||||||||||||||
| KEGG | hsa:55856. | ||||||||||||||||||||||||
| UCSC | uc003nek.1. human. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| CTD | 55856. | ||||||||||||||||||||||||
| GeneCards | GC06P024775. | ||||||||||||||||||||||||
| H-InvDB | HIX0005627. | ||||||||||||||||||||||||
| HGNC | HGNC:20999. ACOT13. | ||||||||||||||||||||||||
| HPA | HPA019881. | ||||||||||||||||||||||||
| PharmGKB | PA128394691. | ||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| HOVERGEN | Q9NPJ3. | ||||||||||||||||||||||||
| InParanoid | Q9NPJ3. | ||||||||||||||||||||||||
| OMA | CTERGAP. | ||||||||||||||||||||||||
| OrthoDB | EOG93N9Z5. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q9NPJ3. | ||||||||||||||||||||||||
| Bgee | Q9NPJ3. | ||||||||||||||||||||||||
| CleanEx | HS_THEM2. | ||||||||||||||||||||||||
| Genevestigator | Q9NPJ3. | ||||||||||||||||||||||||
| GermOnline | ENSG00000112304. Homo sapiens. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR003736. PAAI. IPR006683. Thioestr_supf. [Graphical view] | ||||||||||||||||||||||||
| Pfam | PF03061. 4HBT. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| NextBio | 61135. | ||||||||||||||||||||||||
Entry information
| Entry name | ACO13_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9NPJ3 Secondary accession number(s): O95549 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


