Reviewed, UniProtKB/Swiss-Prot Q9NPI5 (NRK2_HUMAN)

Names and origin

Protein names

Recommended name:
    Nicotinamide riboside kinase 2
      Short name=NmR-K 2
      Short name=NRK 2
    EC=2.7.1.22
Alternative name(s):
    Ribosylnicotinamide kinase 2
      Short name=RNK 2
    Nicotinic acid riboside kinase 2
    EC=2.7.1.n4
    Ribosylnicotinic acid kinase 2
    Integrin beta-1-binding protein 3
    Muscle integrin-binding protein
      Short name=MIBP

Gene names

Name:	ITGB1BP3
Synonyms:	NRK2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). Reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. May play a role in the regulation of terminal myogenesis. Ref.1 Ref.2
Catalytic activity	ATP + N-ribosylnicotinamide = ADP + nicotinamide ribonucleotide. ATP + D-ribosylnicotinate = ADP + nicotinate D-ribonucleotide.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis.
Subunit structure	Monomer By similarity. Interacts with ITGB1 alone or when associated with alpha-7, but not with alpha-5.
Tissue specificity	Predominantly expressed in skeletal muscle and, at a much lower level, in the heart (at protein level). No expression in brain, kidney, liver, lung, pancreas nor placenta. Ref.1
Induction	Down-regulated during myoblast differentiation By similarity.
Sequence similarities	Belongs to the uridine kinase family. NRK subfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.19 mM for nicotinamide riboside (with ATP as cosubstrate) KM=30 mM for nicotinamide riboside (with GTP as cosubstrate) KM=0.11 mM for tiazofurin (with ATP as cosubstrate) KM=0.063 mM for nicotinic acid riboside (with ATP as cosubstrate) KM=1.3 mM for uridine (with ATP as cosubstrate)

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ribosylnicotinamide kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LRP12	Q9Y561	1	EBI-514059,EBI-296693

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NPI5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NPI5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     110-153: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Nicotinamide riboside kinase 2		PRO_0000215894
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity
Sites
	Active site	35	1	Proton acceptor By similarity
	Metal binding	16	1	Magnesium By similarity
	Metal binding	35	1	Magnesium By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	130	1	ATP By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	134	1	ATP By similarity
Natural variations
	Alternative sequence	110 – 153	44	Missing in isoform 2.		VSP_012677
	Natural variant	178	1	E → K: dbSNP rs16992131.		VAR_024549
Experimental info
	Mutagenesis	35	1	D → A: Loss of activity. Ref.7
	Mutagenesis	100	1	E → A: Loss of activity. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 27F8275A596758E7	FASTA	230	26,046
	10 20 30 40 50 60 MKLIVGIGGM TNGGKTTLTN SLLRALPNCC VIHQDDFFKP QDQIAVGEDG FKQWDVLESL 70 80 90 100 110 120 DMEAMLDTVQ AWLSSPQKFA RAHGVSVQPE ASDTHILLLE GFLLYSYKPL VDLYSRRYFL 130 140 150 160 170 180 TVPYEECKWR RSTRNYTVPD PPGLFDGHVW PMYQKYRQEM EANGVEVVYL DGMKSREELF 190 200 210 220 230 REVLEDIQNS LLNRSQESAP SPARPARTQG PGRGCGHRTA RPAASQQDSM
	Isoform 2. Checksum: 8DE757FC52983F0F	FASTA	186	20,638
	10 20 30 40 50 60 MKLIVGIGGM TNGGKTTLTN SLLRALPNCC VIHQDDFFKP QDQIAVGEDG FKQWDVLESL 70 80 90 100 110 120 DMEAMLDTVQ AWLSSPQKFA RAHGVSVQPE ASDTHILLLE GFLLYSYKPQ KYRQEMEANG 130 140 150 160 170 180 VEVVYLDGMK SREELFREVL EDIQNSLLNR SQESAPSPAR PARTQGPGRG CGHRTARPAA SQQDSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel muscle-specific beta 1 integrin binding protein (MIBP) that modulates myogenic differentiation." Li J., Mayne R., Wu C. J. Cell Biol. 147:1391-1398(1999) [PubMed: 10613898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH ITGB1. Tissue: Heart.
[2]	"Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans." Bieganowski P., Brenner C. Cell 117:495-502(2004) [PubMed: 15137942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[3]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[6]	"The muscle integrin binding protein (MIBP) interacts with alpha7beta1 integrin and regulates cell adhesion and laminin matrix deposition." Li J., Rao H., Burkin D., Kaufman S.J., Wu C. Dev. Biol. 261:209-219(2003) [PubMed: 12941630] [Abstract] Cited for: INTERACTION WITH INTEGRIN ALPHA-7/BETA-1.
[7]	"Nicotinamide riboside kinase structures reveal new pathways to NAD+." Tempel W., Rabeh W.M., Bogan K.L., Belenky P., Wojcik M., Seidle H.F., Nedyalkova L., Yang T., Sauve A.A., Park H.-W., Brenner C. PLoS Biol. 5:2220-2230(2007) [PubMed: 17914902] [Abstract] Cited for: SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ASP-35 AND GLU-100.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF190819 mRNA. Translation: AAF26711.1. AY611481 mRNA. Translation: AAT11929.1. AL365377 mRNA. Translation: CAB96949.1. AK001663 mRNA. Translation: BAA91820.1. AK022514 mRNA. Translation: BAB14071.1. BC093637 mRNA. Translation: AAH93637.1. BC101575 mRNA. Translation: AAI01576.1.
IPI	IPI00004401. IPI00009240.
RefSeq	NP_733778.1.
UniGene	Hs.135458
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NPI5. 3 interactions.
STRING	Q9NPI5.
Proteomic databases
PRIDE	Q9NPI5.
Genome annotation databases
Ensembl	ENST00000168977; ENSP00000168977; ENSG00000077009; Homo sapiens. [Genome view] ENST00000395034; ENSP00000378475; ENSG00000077009; Homo sapiens. [Genome view]
GeneID	27231.
KEGG	hsa:27231.
NMPDR	fig|9606.3.peg.15403.
UCSC	uc002lyz.2. human.
Organism-specific databases
CTD	27231.
GeneCards	GC19P003884.
H-InvDB	HIX0014649.
HGNC	HGNC:17871. ITGB1BP3.
MIM	608705. gene.
PharmGKB	PA134938442.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NPI5.
HOVERGEN	Q9NPI5.
OMA	ECKWRRS.
Gene expression databases
ArrayExpress	Q9NPI5.
Bgee	Q9NPI5.
CleanEx	HS_ITGB1BP3.
Genevestigator	Q9NPI5.
GermOnline	ENSG00000077009. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	50087.
SOURCE	Search...

Entry information

Entry name

NRK2_HUMAN

Accession

Primary (citable) accession number: Q9NPI5
Secondary accession number(s): Q52M81, Q9NZK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.