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Q9NPI5 (NRK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nicotinamide riboside kinase 2

Short name=NRK 2
Short name=NmR-K 2
EC=2.7.1.22
Alternative name(s):
Integrin beta-1-binding protein 3
Muscle integrin-binding protein
Short name=MIBP
Nicotinic acid riboside kinase 2
EC=2.7.1.173
Ribosylnicotinamide kinase 2
Short name=RNK 2
Ribosylnicotinic acid kinase 2
Gene names
Name:NMRK2
Synonyms:ITGB1BP3, NRK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). Reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. May play a role in the regulation of terminal myogenesis. Ref.1 Ref.2

Catalytic activity

ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide.

ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis.

Subunit structure

Monomer By similarity. Interacts with ITGB1 alone or when associated with alpha-7, but not with alpha-5. Ref.1 Ref.6

Tissue specificity

Predominantly expressed in skeletal muscle and, at a much lower level, in the heart (at protein level). No expression in brain, kidney, liver, lung, pancreas nor placenta. Ref.1

Induction

Down-regulated during myoblast differentiation By similarity.

Sequence similarities

Belongs to the uridine kinase family. NRK subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=0.19 mM for nicotinamide riboside (with ATP as cosubstrate) Ref.7

KM=30 mM for nicotinamide riboside (with GTP as cosubstrate)

KM=0.11 mM for tiazofurin (with ATP as cosubstrate)

KM=0.063 mM for nicotinic acid riboside (with ATP as cosubstrate)

KM=1.3 mM for uridine (with ATP as cosubstrate)

Sequence caution

The sequence AAF26711.1 differs from that shown. Reason: Aberrant splicing.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LRP12Q9Y5612EBI-514059,EBI-296693

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Nicotinamide riboside kinase 2
PRO_0000215894

Regions

Nucleotide binding9 – 179ATP By similarity

Sites

Active site351Proton acceptor By similarity
Metal binding161Magnesium By similarity
Metal binding351Magnesium By similarity
Binding site551Substrate By similarity
Binding site1301ATP By similarity
Binding site1311Substrate By similarity
Binding site1341ATP By similarity

Natural variations

Natural variant1781E → K.
Corresponds to variant rs16992131 [ dbSNP | Ensembl ].
VAR_024549

Experimental info

Mutagenesis351D → A: Loss of activity. Ref.7
Mutagenesis1001E → A: Loss of activity. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9NPI5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 27F8275A596758E7

FASTA23026,046
        10         20         30         40         50         60 
MKLIVGIGGM TNGGKTTLTN SLLRALPNCC VIHQDDFFKP QDQIAVGEDG FKQWDVLESL 

        70         80         90        100        110        120 
DMEAMLDTVQ AWLSSPQKFA RAHGVSVQPE ASDTHILLLE GFLLYSYKPL VDLYSRRYFL 

       130        140        150        160        170        180 
TVPYEECKWR RSTRNYTVPD PPGLFDGHVW PMYQKYRQEM EANGVEVVYL DGMKSREELF 

       190        200        210        220        230 
REVLEDIQNS LLNRSQESAP SPARPARTQG PGRGCGHRTA RPAASQQDSM 

« Hide

References

« Hide 'large scale' references
[1]"A novel muscle-specific beta 1 integrin binding protein (MIBP) that modulates myogenic differentiation."
Li J., Mayne R., Wu C.
J. Cell Biol. 147:1391-1398(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH ITGB1.
Tissue: Heart.
[2]"Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans."
Bieganowski P., Brenner C.
Cell 117:495-502(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]The European IMAGE consortium
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[6]"The muscle integrin binding protein (MIBP) interacts with alpha7beta1 integrin and regulates cell adhesion and laminin matrix deposition."
Li J., Rao H., Burkin D., Kaufman S.J., Wu C.
Dev. Biol. 261:209-219(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH INTEGRIN ALPHA-7/BETA-1.
[7]"Nicotinamide riboside kinase structures reveal new pathways to NAD+."
Tempel W., Rabeh W.M., Bogan K.L., Belenky P., Wojcik M., Seidle H.F., Nedyalkova L., Yang T., Sauve A.A., Park H.-W., Brenner C.
PLoS Biol. 5:2220-2230(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ASP-35 AND GLU-100.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF190819 mRNA. Translation: AAF26711.1. Sequence problems.
AY611481 mRNA. Translation: AAT11929.1.
AL365377 mRNA. Translation: CAB96949.1.
AK001663 mRNA. Translation: BAA91820.1.
AK022514 mRNA. Translation: BAB14071.1.
BC093637 mRNA. Translation: AAH93637.1.
BC101575 mRNA. Translation: AAI01576.1.
RefSeqNP_733778.1. NM_170678.2.
UniGeneHs.135458.

3D structure databases

ProteinModelPortalQ9NPI5.
SMRQ9NPI5. Positions 3-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118080. 4 interactions.
IntActQ9NPI5. 4 interactions.
MINTMINT-1401053.
STRING9606.ENSP00000168977.

PTM databases

PhosphoSiteQ9NPI5.

Polymorphism databases

DMDM50401178.

Proteomic databases

PaxDbQ9NPI5.
PRIDEQ9NPI5.

Protocols and materials databases

DNASU27231.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000168977; ENSP00000168977; ENSG00000077009.
GeneID27231.
KEGGhsa:27231.
UCSCuc002lyz.4. human.

Organism-specific databases

CTD27231.
GeneCardsGC19P003933.
HGNCHGNC:17871. NMRK2.
HPAHPA049909.
MIM608705. gene.
neXtProtNX_Q9NPI5.
PharmGKBPA134938442.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0572.
HOVERGENHBG052669.
InParanoidQ9NPI5.
KOK10524.
OrthoDBEOG7JQBPF.
PhylomeDBQ9NPI5.
TreeFamTF105395.

Enzyme and pathway databases

UniPathwayUPA00253.

Gene expression databases

ArrayExpressQ9NPI5.
BgeeQ9NPI5.
CleanExHS_ITGB1BP3.
GenevestigatorQ9NPI5.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR026681. NRK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR23404:SF1. PTHR23404:SF1. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

GeneWikiITGB1BP3.
GenomeRNAi27231.
NextBio50087.
PROQ9NPI5.
SOURCESearch...

Entry information

Entry nameNRK2_HUMAN
AccessionPrimary (citable) accession number: Q9NPI5
Secondary accession number(s): Q52M81, Q9NZK3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM