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Protein

Bromodomain-containing protein 7

Gene

BRD7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.By similarity5 Publications

GO - Molecular functioni

  • histone binding Source: MGI
  • lysine-acetylated histone binding Source: UniProtKB
  • p53 binding Source: UniProtKB
  • transcription coactivator activity Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB
  • transcription factor binding Source: MGI
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of histone acetylation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of signal transduction by p53 class mediator Source: Reactome
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Biological processCell cycle, Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-6804758. Regulation of TP53 Activity through Acetylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 7
Alternative name(s):
75 kDa bromodomain protein
Protein CELTIX-1
Gene namesi
Name:BRD7
Synonyms:BP75, CELTIX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14310. BRD7.

Subcellular locationi

GO - Cellular componenti

  • BAF-type complex Source: Ensembl
  • cytoplasm Source: ProtInc
  • cytosol Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi29117.
OpenTargetsiENSG00000166164.
PharmGKBiPA25417.

Chemistry databases

ChEMBLiCHEMBL3085622.
GuidetoPHARMACOLOGYi2726.

Polymorphism and mutation databases

BioMutaiBRD7.
DMDMi74734307.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002276641 – 651Bromodomain-containing protein 7Add BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei279PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Cross-linki305Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei328N6-acetyllysineBy similarity1
Modified residuei380PhosphoserineCombined sources1
Modified residuei482PhosphoserineBy similarity1
Modified residuei514PhosphothreonineCombined sources1
Modified residuei621PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9NPI1.
MaxQBiQ9NPI1.
PaxDbiQ9NPI1.
PeptideAtlasiQ9NPI1.
PRIDEiQ9NPI1.

PTM databases

iPTMnetiQ9NPI1.
PhosphoSitePlusiQ9NPI1.

Expressioni

Gene expression databases

BgeeiENSG00000166164.
CleanExiHS_BRD7.
ExpressionAtlasiQ9NPI1. baseline and differential.
GenevisibleiQ9NPI1. HS.

Organism-specific databases

HPAiCAB046458.
HPA042389.
HPA060171.

Interactioni

Subunit structurei

Interacts with TRIM24, PTPN13 and DVL1. Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D and ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex (By similarity). Interacts with IRF2 and HNRPUL1. Interacts (via N-terminus) with TP53. Interacts (via C-terminus) with EP300. Interacts with BRCA1. Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at 'Lys-14' (H3K14ac). Has low affinity for histone H3 acetylated at 'Lys-9' (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at 'Lys-9' (H3K9ac) and at 'Lys-14' (H3K14ac). Has very low affinity for non-acetylated histone H3. Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at 'Lys-8' (H3K8ac) (in vitro).By similarity7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • histone binding Source: MGI
  • lysine-acetylated histone binding Source: UniProtKB
  • p53 binding Source: UniProtKB
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi118883. 46 interactors.
DIPiDIP-32509N.
IntActiQ9NPI1. 28 interactors.
MINTiMINT-1383082.
STRINGi9606.ENSP00000378181.

Chemistry databases

BindingDBiQ9NPI1.

Structurei

Secondary structure

1651
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi133 – 148Combined sources16
Helixi152 – 154Combined sources3
Beta strandi156 – 158Combined sources3
Turni162 – 164Combined sources3
Beta strandi165 – 167Combined sources3
Helixi168 – 171Combined sources4
Helixi178 – 185Combined sources8
Turni186 – 188Combined sources3
Helixi193 – 209Combined sources17
Beta strandi213 – 216Combined sources4
Helixi217 – 235Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I7KNMR-A129-236[»]
ProteinModelPortaliQ9NPI1.
SMRiQ9NPI1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NPI1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 218BromoPROSITE-ProRule annotationAdd BLAST71

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili536 – 567Sequence analysisAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi65 – 96Nuclear localization signal1 PublicationAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 91Lys-richAdd BLAST89

Keywords - Domaini

Bromodomain, Coiled coil

Phylogenomic databases

eggNOGiKOG1828. Eukaryota.
ENOG410XRRI. LUCA.
GeneTreeiENSGT00530000063939.
HOGENOMiHOG000070022.
HOVERGENiHBG071934.
InParanoidiQ9NPI1.
KOiK11723.
OMAiCQAPVRL.
OrthoDBiEOG091G06S4.
PhylomeDBiQ9NPI1.
TreeFamiTF106439.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiView protein in InterPro
IPR001487. Bromodomain.
IPR021900. DUF3512.
PfamiView protein in Pfam
PF00439. Bromodomain. 1 hit.
PF12024. DUF3512. 1 hit.
PRINTSiPR00503. BROMODOMAIN.
SMARTiView protein in SMART
SM00297. BROMO. 1 hit.
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiView protein in PROSITE
PS50014. BROMODOMAIN_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NPI1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKHKKHKS DKHLYEEYVE KPLKLVLKVG GNEVTELSTG SSGHDSSLFE
60 70 80 90 100
DKNDHDKHKD RKRKKRKKGE KQIPGEEKGR KRRRVKEDKK KRDRDRVENE
110 120 130 140 150
AEKDLQCHAP VRLDLPPEKP LTSSLAKQEE VEQTPLQEAL NQLMRQLQRK
160 170 180 190 200
DPSAFFSFPV TDFIAPGYSM IIKHPMDFST MKEKIKNNDY QSIEELKDNF
210 220 230 240 250
KLMCTNAMIY NKPETIYYKA AKKLLHSGMK ILSQERIQSL KQSIDFMADL
260 270 280 290 300
QKTRKQKDGT DTSQSGEDGG CWQREREDSG DAEAHAFKSP SKENKKKDKD
310 320 330 340 350
MLEDKFKSNN LEREQEQLDR IVKESGGKLT RRLVNSQCEF ERRKPDGTTT
360 370 380 390 400
LGLLHPVDPI VGEPGYCPVR LGMTTGRLQS GVNTLQGFKE DKRNKVTPVL
410 420 430 440 450
YLNYGPYSSY APHYDSTFAN ISKDDSDLIY STYGEDSDLP SDFSIHEFLA
460 470 480 490 500
TCQDYPYVMA DSLLDVLTKG GHSRTLQEME MSLPEDEGHT RTLDTAKEME
510 520 530 540 550
ITEVEPPGRL DSSTQDRLIA LKAVTNFGVP VEVFDSEEAE IFQKKLDETT
560 570 580 590 600
RLLRELQEAQ NERLSTRPPP NMICLLGPSY REMHLAEQVT NNLKELAQQV
610 620 630 640 650
TPGDIVSTYG VRKAMGISIP SPVMENNFVD LTEDTEEPKK TDVAECGPGG

S
Length:651
Mass (Da):74,139
Last modified:October 1, 2000 - v1
Checksum:i29B7947644C215E7
GO
Isoform 2 (identifier: Q9NPI1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-500: E → EQ

Show »
Length:652
Mass (Da):74,267
Checksum:iAE7A8BB828D0B538
GO

Sequence cautioni

The sequence AAH01611 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB55031 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC11089 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66R → G in AAF19526 (PubMed:11025449).Curated1
Sequence conflicti83R → T in AAF19526 (PubMed:11025449).Curated1
Sequence conflicti96R → Q in AAF19526 (PubMed:11025449).Curated1
Sequence conflicti170M → I in AAH94706 (PubMed:15489334).Curated1
Sequence conflicti485E → G in BAB55031 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017564500E → EQ in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF213969 mRNA. Translation: AAF19526.1.
AJ271881 mRNA. Translation: CAB72445.1.
AF152604 mRNA. Translation: AAF75126.1.
BC001611 mRNA. Translation: AAH01611.1. Different initiation.
AC007493 Genomic DNA. No translation available.
AC007597 Genomic DNA. No translation available.
AC023826 Genomic DNA. No translation available.
BC050728 mRNA. Translation: AAH50728.1.
BC094706 mRNA. Translation: AAH94706.1.
AK027308 mRNA. Translation: BAB55031.1. Different initiation.
AK074613 mRNA. Translation: BAC11089.1. Different initiation.
CCDSiCCDS10742.1. [Q9NPI1-1]
CCDS54007.1. [Q9NPI1-2]
RefSeqiNP_001167455.1. NM_001173984.2. [Q9NPI1-2]
NP_037395.2. NM_013263.4. [Q9NPI1-1]
UniGeneiHs.437894.

Genome annotation databases

EnsembliENST00000394688; ENSP00000378180; ENSG00000166164. [Q9NPI1-1]
ENST00000394689; ENSP00000378181; ENSG00000166164. [Q9NPI1-2]
GeneIDi29117.
KEGGihsa:29117.
UCSCiuc002ege.3. human. [Q9NPI1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF213969 mRNA. Translation: AAF19526.1.
AJ271881 mRNA. Translation: CAB72445.1.
AF152604 mRNA. Translation: AAF75126.1.
BC001611 mRNA. Translation: AAH01611.1. Different initiation.
AC007493 Genomic DNA. No translation available.
AC007597 Genomic DNA. No translation available.
AC023826 Genomic DNA. No translation available.
BC050728 mRNA. Translation: AAH50728.1.
BC094706 mRNA. Translation: AAH94706.1.
AK027308 mRNA. Translation: BAB55031.1. Different initiation.
AK074613 mRNA. Translation: BAC11089.1. Different initiation.
CCDSiCCDS10742.1. [Q9NPI1-1]
CCDS54007.1. [Q9NPI1-2]
RefSeqiNP_001167455.1. NM_001173984.2. [Q9NPI1-2]
NP_037395.2. NM_013263.4. [Q9NPI1-1]
UniGeneiHs.437894.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I7KNMR-A129-236[»]
ProteinModelPortaliQ9NPI1.
SMRiQ9NPI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118883. 46 interactors.
DIPiDIP-32509N.
IntActiQ9NPI1. 28 interactors.
MINTiMINT-1383082.
STRINGi9606.ENSP00000378181.

Chemistry databases

BindingDBiQ9NPI1.
ChEMBLiCHEMBL3085622.
GuidetoPHARMACOLOGYi2726.

PTM databases

iPTMnetiQ9NPI1.
PhosphoSitePlusiQ9NPI1.

Polymorphism and mutation databases

BioMutaiBRD7.
DMDMi74734307.

Proteomic databases

EPDiQ9NPI1.
MaxQBiQ9NPI1.
PaxDbiQ9NPI1.
PeptideAtlasiQ9NPI1.
PRIDEiQ9NPI1.

Protocols and materials databases

DNASUi29117.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394688; ENSP00000378180; ENSG00000166164. [Q9NPI1-1]
ENST00000394689; ENSP00000378181; ENSG00000166164. [Q9NPI1-2]
GeneIDi29117.
KEGGihsa:29117.
UCSCiuc002ege.3. human. [Q9NPI1-1]

Organism-specific databases

CTDi29117.
DisGeNETi29117.
GeneCardsiBRD7.
HGNCiHGNC:14310. BRD7.
HPAiCAB046458.
HPA042389.
HPA060171.
neXtProtiNX_Q9NPI1.
OpenTargetsiENSG00000166164.
PharmGKBiPA25417.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1828. Eukaryota.
ENOG410XRRI. LUCA.
GeneTreeiENSGT00530000063939.
HOGENOMiHOG000070022.
HOVERGENiHBG071934.
InParanoidiQ9NPI1.
KOiK11723.
OMAiCQAPVRL.
OrthoDBiEOG091G06S4.
PhylomeDBiQ9NPI1.
TreeFamiTF106439.

Enzyme and pathway databases

ReactomeiR-HSA-6804758. Regulation of TP53 Activity through Acetylation.

Miscellaneous databases

ChiTaRSiBRD7. human.
EvolutionaryTraceiQ9NPI1.
GeneWikiiBRD7.
GenomeRNAii29117.
PROiQ9NPI1.

Gene expression databases

BgeeiENSG00000166164.
CleanExiHS_BRD7.
ExpressionAtlasiQ9NPI1. baseline and differential.
GenevisibleiQ9NPI1. HS.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiView protein in InterPro
IPR001487. Bromodomain.
IPR021900. DUF3512.
PfamiView protein in Pfam
PF00439. Bromodomain. 1 hit.
PF12024. DUF3512. 1 hit.
PRINTSiPR00503. BROMODOMAIN.
SMARTiView protein in SMART
SM00297. BROMO. 1 hit.
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiView protein in PROSITE
PS50014. BROMODOMAIN_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBRD7_HUMAN
AccessioniPrimary (citable) accession number: Q9NPI1
Secondary accession number(s): Q4VC09
, Q8N2L9, Q96KA4, Q9BV48, Q9UH59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 1, 2000
Last modified: March 15, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.