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Protein

Neuroglobin

Gene

NGB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Iron (heme distal ligand)Combined sources1 Publication1
Metal bindingi96Iron (heme proximal ligand)Combined sources1 Publication1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • oxygen binding Source: InterPro
  • oxygen transporter activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • oxygen transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Oxygen transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000165553-MONOMER.

Protein family/group databases

TCDBi1.A.107.1.4. the pore-forming globin (globin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroglobin
Gene namesi
Name:NGB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:14077. NGB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi58157.
OpenTargetsiENSG00000165553.
PharmGKBiPA31612.

Polymorphism and mutation databases

BioMutaiNGB.
DMDMi32171399.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000533901 – 151NeuroglobinAdd BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 55Redox-active2 Publications

Post-translational modificationi

A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9NPG2.
PeptideAtlasiQ9NPG2.
PRIDEiQ9NPG2.

Expressioni

Tissue specificityi

Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus.1 Publication

Gene expression databases

BgeeiENSG00000165553.
CleanExiHS_NGB.
ExpressionAtlasiQ9NPG2. baseline and differential.
GenevisibleiQ9NPG2. HS.

Organism-specific databases

HPAiCAB037319.
HPA042615.
HPA058596.

Interactioni

Subunit structurei

Monomer. Homodimer and homotetramer; disulfide-linked (By similarity). Interacts with 14-3-3.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
C1orf94Q6P1W53EBI-10311409,EBI-946029
CCDC36Q8IYA85EBI-10311409,EBI-8638439
L3MBTL3Q96JM73EBI-10311409,EBI-2686809
RELQ048643EBI-10311409,EBI-307352
TDO2P487755EBI-10311409,EBI-743494
VAC14Q08AM65EBI-10311409,EBI-2107455

Protein-protein interaction databases

BioGridi121798. 12 interactors.
IntActiQ9NPG2. 7 interactors.
STRINGi9606.ENSP00000298352.

Structurei

Secondary structure

1151
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 13Combined sources8
Turni14 – 18Combined sources5
Helixi20 – 34Combined sources15
Helixi38 – 49Combined sources12
Helixi55 – 57Combined sources3
Helixi59 – 77Combined sources19
Helixi79 – 85Combined sources7
Helixi86 – 98Combined sources13
Helixi105 – 121Combined sources17
Helixi122 – 124Combined sources3
Helixi127 – 144Combined sources18
Helixi145 – 148Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OJ6X-ray1.95A/B/C/D1-151[»]
4MPMX-ray1.74A/B1-151[»]
ProteinModelPortaliQ9NPG2.
SMRiQ9NPG2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NPG2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149GlobinAdd BLAST149

Sequence similaritiesi

Belongs to the globin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3378. Eukaryota.
COG1018. LUCA.
GeneTreeiENSGT00510000048375.
HOGENOMiHOG000036573.
HOVERGENiHBG039321.
InParanoidiQ9NPG2.
OMAiFASPQEC.
OrthoDBiEOG091G0R7W.
PhylomeDBiQ9NPG2.
TreeFamiTF333247.

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NPG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS
60 70 80 90 100
SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG
110 120 130 140 150
VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG

E
Length:151
Mass (Da):16,933
Last modified:October 1, 2000 - v1
Checksum:i45A292A7D77B9CE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245944 Genomic DNA. Translation: CAC12994.1.
AJ245946 mRNA. Translation: CAC11133.1.
AF422796 Genomic DNA. Translation: AAL98923.1.
AF422797 mRNA. Translation: AAL98924.1.
AC007375 Genomic DNA. Translation: AAF63183.1.
AC007954 Genomic DNA. Translation: AAF62557.1.
BC032509 mRNA. Translation: AAH32509.1.
CCDSiCCDS9856.1.
RefSeqiNP_067080.1. NM_021257.3.
UniGeneiHs.274363.

Genome annotation databases

EnsembliENST00000298352; ENSP00000298352; ENSG00000165553.
GeneIDi58157.
KEGGihsa:58157.
UCSCiuc001xtg.2. human.

Cross-referencesi

Web resourcesi

Wikipedia

Neuroglobin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245944 Genomic DNA. Translation: CAC12994.1.
AJ245946 mRNA. Translation: CAC11133.1.
AF422796 Genomic DNA. Translation: AAL98923.1.
AF422797 mRNA. Translation: AAL98924.1.
AC007375 Genomic DNA. Translation: AAF63183.1.
AC007954 Genomic DNA. Translation: AAF62557.1.
BC032509 mRNA. Translation: AAH32509.1.
CCDSiCCDS9856.1.
RefSeqiNP_067080.1. NM_021257.3.
UniGeneiHs.274363.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OJ6X-ray1.95A/B/C/D1-151[»]
4MPMX-ray1.74A/B1-151[»]
ProteinModelPortaliQ9NPG2.
SMRiQ9NPG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121798. 12 interactors.
IntActiQ9NPG2. 7 interactors.
STRINGi9606.ENSP00000298352.

Protein family/group databases

TCDBi1.A.107.1.4. the pore-forming globin (globin) family.

Polymorphism and mutation databases

BioMutaiNGB.
DMDMi32171399.

Proteomic databases

PaxDbiQ9NPG2.
PeptideAtlasiQ9NPG2.
PRIDEiQ9NPG2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298352; ENSP00000298352; ENSG00000165553.
GeneIDi58157.
KEGGihsa:58157.
UCSCiuc001xtg.2. human.

Organism-specific databases

CTDi58157.
DisGeNETi58157.
GeneCardsiNGB.
HGNCiHGNC:14077. NGB.
HPAiCAB037319.
HPA042615.
HPA058596.
MIMi605304. gene.
neXtProtiNX_Q9NPG2.
OpenTargetsiENSG00000165553.
PharmGKBiPA31612.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3378. Eukaryota.
COG1018. LUCA.
GeneTreeiENSGT00510000048375.
HOGENOMiHOG000036573.
HOVERGENiHBG039321.
InParanoidiQ9NPG2.
OMAiFASPQEC.
OrthoDBiEOG091G0R7W.
PhylomeDBiQ9NPG2.
TreeFamiTF333247.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000165553-MONOMER.

Miscellaneous databases

ChiTaRSiNGB. human.
EvolutionaryTraceiQ9NPG2.
GenomeRNAii58157.
PROiQ9NPG2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165553.
CleanExiHS_NGB.
ExpressionAtlasiQ9NPG2. baseline and differential.
GenevisibleiQ9NPG2. HS.

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNGB_HUMAN
AccessioniPrimary (citable) accession number: Q9NPG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.