Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arf-GAP with dual PH domain-containing protein 2

Gene

ADAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity.Curated1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  • phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
  • protein binding, bridging Source: UniProtKB

GO - Biological processi

  • heart development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with dual PH domain-containing protein 2
Alternative name(s):
Centaurin-alpha-2
Short name:
Cnt-a2
Gene namesi
Name:ADAP2
Synonyms:CENTA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:16487. ADAP2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication

  • Note: Constitutively associated with the plasma membrane. Excluded from the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • mitochondrial envelope Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26405.

Polymorphism and mutation databases

BioMutaiADAP2.
DMDMi27923749.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Arf-GAP with dual PH domain-containing protein 2PRO_0000074206Add
BLAST

Proteomic databases

MaxQBiQ9NPF8.
PaxDbiQ9NPF8.
PRIDEiQ9NPF8.

PTM databases

PhosphoSiteiQ9NPF8.

Expressioni

Tissue specificityi

Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow.1 Publication

Gene expression databases

BgeeiQ9NPF8.
CleanExiHS_ADAP2.
ExpressionAtlasiQ9NPF8. baseline and differential.
GenevisibleiQ9NPF8. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
IKBKGQ9Y6K92EBI-718895,EBI-81279

Protein-protein interaction databases

BioGridi120915. 11 interactions.
IntActiQ9NPF8. 3 interactions.
MINTiMINT-1407535.
STRINGi9606.ENSP00000329468.

Structurei

3D structure databases

ProteinModelPortaliQ9NPF8.
SMRiQ9NPF8. Positions 4-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 131123Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini132 – 233102PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini255 – 361107PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00530000062917.
HOGENOMiHOG000006719.
HOVERGENiHBG050888.
InParanoidiQ9NPF8.
PhylomeDBiQ9NPF8.
TreeFamiTF324540.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001164. ArfGAP.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NPF8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG
60 70 80 90 100
VHRNFPDISR VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP
110 120 130 140 150
QANDCLVLKE QWIRAKYERR EFMADGETIS LPGNREGFLW KRGRDNSQFL
160 170 180 190 200
RRKFVLLARE GLLKYFTKEQ GKSPKAVISI KDLNATFQTE KIGHPHGLQI
210 220 230 240 250
TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF PELPESELVP
260 270 280 290 300
FLTRNYLKQG FMEKTGPKQK EPFKKRWFAL DCHERRLLYY KNPLDAFEQG
310 320 330 340 350
QVFLGNKEQG YEAYEDLPKG IRGNRWKAGL TIVTPERRFV LTCPSEKEQQ
360 370 380
EWLESLRGVL SSPLTPLNRL TASTESGRSS R
Length:381
Mass (Da):44,349
Last modified:October 1, 2000 - v1
Checksum:i4FAE208072A92C01
GO
Isoform 2 (identifier: Q9NPF8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     269-269: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:380
Mass (Da):44,221
Checksum:i86654BC0B1CECBF7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121L → P in CAC40651 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei269 – 2691Missing in isoform 2. 1 PublicationVSP_011180

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238994 mRNA. Translation: CAB88383.1.
AJ272195 mRNA. Translation: CAB77266.1.
AJ242782 mRNA. Translation: CAC40651.1.
BC033758 mRNA. Translation: AAH33758.1.
CCDSiCCDS11261.1. [Q9NPF8-1]
RefSeqiNP_060874.1. NM_018404.2. [Q9NPF8-1]
UniGeneiHs.514063.

Genome annotation databases

EnsembliENST00000330889; ENSP00000329468; ENSG00000184060. [Q9NPF8-1]
GeneIDi55803.
KEGGihsa:55803.
UCSCiuc002hfx.3. human. [Q9NPF8-1]
uc002hfy.3. human. [Q9NPF8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238994 mRNA. Translation: CAB88383.1.
AJ272195 mRNA. Translation: CAB77266.1.
AJ242782 mRNA. Translation: CAC40651.1.
BC033758 mRNA. Translation: AAH33758.1.
CCDSiCCDS11261.1. [Q9NPF8-1]
RefSeqiNP_060874.1. NM_018404.2. [Q9NPF8-1]
UniGeneiHs.514063.

3D structure databases

ProteinModelPortaliQ9NPF8.
SMRiQ9NPF8. Positions 4-366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120915. 11 interactions.
IntActiQ9NPF8. 3 interactions.
MINTiMINT-1407535.
STRINGi9606.ENSP00000329468.

PTM databases

PhosphoSiteiQ9NPF8.

Polymorphism and mutation databases

BioMutaiADAP2.
DMDMi27923749.

Proteomic databases

MaxQBiQ9NPF8.
PaxDbiQ9NPF8.
PRIDEiQ9NPF8.

Protocols and materials databases

DNASUi55803.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330889; ENSP00000329468; ENSG00000184060. [Q9NPF8-1]
GeneIDi55803.
KEGGihsa:55803.
UCSCiuc002hfx.3. human. [Q9NPF8-1]
uc002hfy.3. human. [Q9NPF8-2]

Organism-specific databases

CTDi55803.
GeneCardsiGC17P029248.
HGNCiHGNC:16487. ADAP2.
MIMi608635. gene.
neXtProtiNX_Q9NPF8.
PharmGKBiPA26405.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00530000062917.
HOGENOMiHOG000006719.
HOVERGENiHBG050888.
InParanoidiQ9NPF8.
PhylomeDBiQ9NPF8.
TreeFamiTF324540.

Miscellaneous databases

ChiTaRSiADAP2. human.
GeneWikiiCENTA2.
GenomeRNAii55803.
NextBioi60951.
PROiQ9NPF8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NPF8.
CleanExiHS_ADAP2.
ExpressionAtlasiQ9NPF8. baseline and differential.
GenevisibleiQ9NPF8. HS.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001164. ArfGAP.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle."
    Whitley P., Gibbard A.M., Koumanov F., Oldfield S., Kilgour E.E., Prestwich G.D., Holman G.D.
    Eur. J. Cell Biol. 81:222-230(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions."
    Jenne D.E., Tinschert S., Stegmann E., Reimann H., Nuernberg P., Horn D., Naumann I., Buske A., Thiel G.
    Genomics 66:93-97(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. Bertsch U., Illies C., Mayr G.W.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Leukocyte.
  5. "Identification of gene structure and subcellular localization of human centaurin alpha 2, and p42IP4, a family of two highly homologous, Ins 1,3,4,5-P4-/PtdIns 3,4,5-P3-binding, adapter proteins."
    Hanck T., Stricker R., Sedehizade F., Reiser G.
    J. Neurochem. 88:326-336(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiADAP2_HUMAN
AccessioniPrimary (citable) accession number: Q9NPF8
Secondary accession number(s): Q8N4Q6, Q96SD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.