Q9NPF8 (ADAP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arf-GAP with dual PH domain-containing protein 2

Alternative name(s):
Centaurin-alpha-2
Short name=Cnt-a2

Gene names

Name:	ADAP2
Synonyms:	CENTA2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GTPase-activating protein for the ADP ribosylation factor family Potential. Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity. Ref.5
Subcellular location	Cytoplasm. Cell membrane. Note: Constitutively associated with the plasma membrane. Excluded from the nucleus. Ref.5
Tissue specificity	Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow. Ref.5
Sequence similarities	Contains 1 Arf-GAP domain. Contains 2 PH domains.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heart development Inferred from expression pattern. Source: UniProtKB regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial envelope Inferred from sequence or structural similarity Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay Ref.5. Source: UniProtKB
Molecular function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro inositol 1,3,4,5 tetrakisphosphate binding Inferred from direct assay Ref.5. Source: UniProtKB phosphatidylinositol-3,4,5-trisphosphate binding Inferred from direct assay Ref.5. Source: UniProtKB phosphatidylinositol-3,4-bisphosphate binding Inferred from sequence or structural similarity Ref.1. Source: UniProtKB phosphatidylinositol-4,5-bisphosphate binding Inferred from sequence or structural similarity Ref.1. Source: UniProtKB protein binding, bridging Non-traceable author statement Ref.5. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NPF8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NPF8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     269-269: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Arf-GAP with dual PH domain-containing protein 2		PRO_0000074206
Regions
	Domain	9 – 131	123	Arf-GAP
	Domain	132 – 233	102	PH 1
	Domain	255 – 361	107	PH 2
	Zinc finger	25 – 48	24	C4-type
Natural variations
	Alternative sequence	269	1	Missing in isoform 2.		VSP_011180
Experimental info
	Sequence conflict	12	1	L → P in CAC40651. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4FAE208072A92C01	FASTA	381	44,349
	10 20 30 40 50 60 MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG VHRNFPDISR 70 80 90 100 110 120 VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP QANDCLVLKE QWIRAKYERR 130 140 150 160 170 180 EFMADGETIS LPGNREGFLW KRGRDNSQFL RRKFVLLARE GLLKYFTKEQ GKSPKAVISI 190 200 210 220 230 240 KDLNATFQTE KIGHPHGLQI TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF 250 260 270 280 290 300 PELPESELVP FLTRNYLKQG FMEKTGPKQK EPFKKRWFAL DCHERRLLYY KNPLDAFEQG 310 320 330 340 350 360 QVFLGNKEQG YEAYEDLPKG IRGNRWKAGL TIVTPERRFV LTCPSEKEQQ EWLESLRGVL 370 380 SSPLTPLNRL TASTESGRSS R
	Isoform 2 [UniParc]. Checksum: 86654BC0B1CECBF7	FASTA	380	44,221
	10 20 30 40 50 60 MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG VHRNFPDISR 70 80 90 100 110 120 VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP QANDCLVLKE QWIRAKYERR 130 140 150 160 170 180 EFMADGETIS LPGNREGFLW KRGRDNSQFL RRKFVLLARE GLLKYFTKEQ GKSPKAVISI 190 200 210 220 230 240 KDLNATFQTE KIGHPHGLQI TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF 250 260 270 280 290 300 PELPESELVP FLTRNYLKQG FMEKTGPKKE PFKKRWFALD CHERRLLYYK NPLDAFEQGQ 310 320 330 340 350 360 VFLGNKEQGY EAYEDLPKGI RGNRWKAGLT IVTPERRFVL TCPSEKEQQE WLESLRGVLS 370 380 SPLTPLNRLT ASTESGRSSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle." Whitley P., Gibbard A.M., Koumanov F., Oldfield S., Kilgour E.E., Prestwich G.D., Holman G.D. Eur. J. Cell Biol. 81:222-230(2002) [PubMed: 12018390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions." Jenne D.E., Tinschert S., Stegmann E., Reimann H., Nuernberg P., Horn D., Naumann I., Buske A., Thiel G. Genomics 66:93-97(2000) [PubMed: 10843809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	Bertsch U., Illies C., Mayr G.W. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Leukocyte.
[5]	"Identification of gene structure and subcellular localization of human centaurin alpha 2, and p42IP4, a family of two highly homologous, Ins 1,3,4,5-P4-/PtdIns 3,4,5-P3-binding, adapter proteins." Hanck T., Stricker R., Sedehizade F., Reiser G. J. Neurochem. 88:326-336(2004) [PubMed: 14690521] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ238994 mRNA. Translation: CAB88383.1. AJ272195 mRNA. Translation: CAB77266.1. AJ242782 mRNA. Translation: CAC40651.1. BC033758 mRNA. Translation: AAH33758.1.
IPI	IPI00020090. IPI00456640.
RefSeq	NP_060874.1. NM_018404.2.
UniGene	Hs.514063.
3D structure databases
ProteinModelPortal	Q9NPF8.
SMR	Q9NPF8. Positions 3-372.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NPF8. 2 interactions.
MINT	MINT-1407535.
STRING	Q9NPF8.
Polymorphism databases
DMDM	27923749.
Proteomic databases
PRIDE	Q9NPF8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000330889; ENSP00000329468; ENSG00000184060.
GeneID	55803.
KEGG	hsa:55803.
UCSC	uc002hfx.1. human. uc002hfy.1. human.
Organism-specific databases
CTD	55803.
GeneCards	GC17P029248.
H-InvDB	HIX0013699.
HGNC	HGNC:16487. ADAP2.
MIM	608635. gene.
neXtProt	NX_Q9NPF8.
PharmGKB	PA164714924. PA26405.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00530000062917.
HOVERGEN	HBG050888.
OrthoDB	EOG4P8FJB.
PhylomeDB	Q9NPF8.
Gene expression databases
ArrayExpress	Q9NPF8.
Bgee	Q9NPF8.
CleanEx	HS_ADAP2.
Genevestigator	Q9NPF8.
GermOnline	ENSG00000184060. Homo sapiens.
Family and domain databases
InterPro	IPR001164. ArfGAP. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 2 hits.
Pfam	PF01412. ArfGap. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00405. REVINTRACTNG.
SMART	SM00105. ArfGap. 1 hit. SM00233. PH. 2 hits. [Graphical view]
SUPFAM	SSF57863. ArfGAP. 1 hit.
PROSITE	PS50115. ARFGAP. 1 hit. PS50003. PH_DOMAIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	60951.
SOURCE	Search...

Entry information

Entry name

ADAP2_HUMAN

Accession

Primary (citable) accession number: Q9NPF8
Secondary accession number(s): Q8N4Q6, Q96SD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.