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Q9NPF8 (ADAP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arf-GAP with dual PH domain-containing protein 2
Alternative name(s):
Centaurin-alpha-2
Short name=Cnt-a2
Gene names
Name:ADAP2
Synonyms:CENTA2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein for the ADP ribosylation factor family Potential. Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity. Ref.5

Subcellular location

Cytoplasm. Cell membrane. Note: Constitutively associated with the plasma membrane. Excluded from the nucleus. Ref.5

Tissue specificity

Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow. Ref.5

Sequence similarities

Contains 1 Arf-GAP domain.

Contains 2 PH domains.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionGTPase activation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processheart development

Inferred from expression pattern PubMed 16138909. Source: UniProtKB

regulation of ARF GTPase activity

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from direct assay Ref.5. Source: UniProtKB

mitochondrial envelope

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

plasma membrane

Inferred from direct assay Ref.5. Source: UniProtKB

   Molecular_functionARF GTPase activator activity

Inferred from electronic annotation. Source: InterPro

inositol 1,3,4,5 tetrakisphosphate binding

Inferred from direct assay Ref.5. Source: UniProtKB

phosphatidylinositol-3,4,5-trisphosphate binding

Inferred from direct assay Ref.5. Source: UniProtKB

phosphatidylinositol-3,4-bisphosphate binding

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

phosphatidylinositol-4,5-bisphosphate binding

Inferred from sequence or structural similarity Ref.1. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 20098747. Source: IntAct

protein binding, bridging

Non-traceable author statement Ref.5. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

IKBKGQ9Y6K92EBI-718895,EBI-81279

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NPF8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NPF8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     269-269: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Arf-GAP with dual PH domain-containing protein 2
PRO_0000074206

Regions

Domain9 – 131123Arf-GAP
Domain132 – 233102PH 1
Domain255 – 361107PH 2
Zinc finger25 – 4824C4-type

Natural variations

Alternative sequence2691Missing in isoform 2.
VSP_011180

Experimental info

Sequence conflict121L → P in CAC40651. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 4FAE208072A92C01

FASTA38144,349
        10         20         30         40         50         60 
MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG VHRNFPDISR 

        70         80         90        100        110        120 
VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP QANDCLVLKE QWIRAKYERR 

       130        140        150        160        170        180 
EFMADGETIS LPGNREGFLW KRGRDNSQFL RRKFVLLARE GLLKYFTKEQ GKSPKAVISI 

       190        200        210        220        230        240 
KDLNATFQTE KIGHPHGLQI TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF 

       250        260        270        280        290        300 
PELPESELVP FLTRNYLKQG FMEKTGPKQK EPFKKRWFAL DCHERRLLYY KNPLDAFEQG 

       310        320        330        340        350        360 
QVFLGNKEQG YEAYEDLPKG IRGNRWKAGL TIVTPERRFV LTCPSEKEQQ EWLESLRGVL 

       370        380 
SSPLTPLNRL TASTESGRSS R 

« Hide

Isoform 2 [UniParc].

Checksum: 86654BC0B1CECBF7
Show »

FASTA38044,221

References

« Hide 'large scale' references
[1]"Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle."
Whitley P., Gibbard A.M., Koumanov F., Oldfield S., Kilgour E.E., Prestwich G.D., Holman G.D.
Eur. J. Cell Biol. 81:222-230(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions."
Jenne D.E., Tinschert S., Stegmann E., Reimann H., Nuernberg P., Horn D., Naumann I., Buske A., Thiel G.
Genomics 66:93-97(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]Bertsch U., Illies C., Mayr G.W.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Leukocyte.
[5]"Identification of gene structure and subcellular localization of human centaurin alpha 2, and p42IP4, a family of two highly homologous, Ins 1,3,4,5-P4-/PtdIns 3,4,5-P3-binding, adapter proteins."
Hanck T., Stricker R., Sedehizade F., Reiser G.
J. Neurochem. 88:326-336(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ238994 mRNA. Translation: CAB88383.1.
AJ272195 mRNA. Translation: CAB77266.1.
AJ242782 mRNA. Translation: CAC40651.1.
BC033758 mRNA. Translation: AAH33758.1.
CCDSCCDS11261.1. [Q9NPF8-1]
RefSeqNP_060874.1. NM_018404.2. [Q9NPF8-1]
UniGeneHs.514063.

3D structure databases

ProteinModelPortalQ9NPF8.
SMRQ9NPF8. Positions 4-366.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120915. 7 interactions.
IntActQ9NPF8. 3 interactions.
MINTMINT-1407535.
STRING9606.ENSP00000329468.

PTM databases

PhosphoSiteQ9NPF8.

Polymorphism databases

DMDM27923749.

Proteomic databases

MaxQBQ9NPF8.
PaxDbQ9NPF8.
PRIDEQ9NPF8.

Protocols and materials databases

DNASU55803.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000330889; ENSP00000329468; ENSG00000184060. [Q9NPF8-1]
GeneID55803.
KEGGhsa:55803.
UCSCuc002hfx.3. human. [Q9NPF8-1]
uc002hfy.3. human. [Q9NPF8-2]

Organism-specific databases

CTD55803.
GeneCardsGC17P029248.
HGNCHGNC:16487. ADAP2.
MIM608635. gene.
neXtProtNX_Q9NPF8.
PharmGKBPA26405.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5347.
HOGENOMHOG000006719.
HOVERGENHBG050888.
PhylomeDBQ9NPF8.
TreeFamTF324540.

Gene expression databases

ArrayExpressQ9NPF8.
BgeeQ9NPF8.
CleanExHS_ADAP2.
GenevestigatorQ9NPF8.

Family and domain databases

Gene3D2.30.29.30. 2 hits.
InterProIPR001164. ArfGAP.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00105. ArfGap. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
PROSITEPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCENTA2.
GenomeRNAi55803.
NextBio60951.
PROQ9NPF8.
SOURCESearch...

Entry information

Entry nameADAP2_HUMAN
AccessionPrimary (citable) accession number: Q9NPF8
Secondary accession number(s): Q8N4Q6, Q96SD5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM