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Q9NPF8

- ADAP2_HUMAN

UniProt

Q9NPF8 - ADAP2_HUMAN

Protein

Arf-GAP with dual PH domain-containing protein 2

Gene

ADAP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    GTPase-activating protein for the ADP ribosylation factor family Potential. Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity.1 PublicationCurated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ARF GTPase activator activity Source: InterPro
    2. inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
    3. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
    4. phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
    5. phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
    6. protein binding Source: IntAct
    7. protein binding, bridging Source: UniProtKB
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. heart development Source: UniProtKB
    2. regulation of ARF GTPase activity Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arf-GAP with dual PH domain-containing protein 2
    Alternative name(s):
    Centaurin-alpha-2
    Short name:
    Cnt-a2
    Gene namesi
    Name:ADAP2
    Synonyms:CENTA2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:16487. ADAP2.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell membrane 1 Publication
    Note: Constitutively associated with the plasma membrane. Excluded from the nucleus.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. mitochondrial envelope Source: UniProtKB
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26405.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 381381Arf-GAP with dual PH domain-containing protein 2PRO_0000074206Add
    BLAST

    Proteomic databases

    MaxQBiQ9NPF8.
    PaxDbiQ9NPF8.
    PRIDEiQ9NPF8.

    PTM databases

    PhosphoSiteiQ9NPF8.

    Expressioni

    Tissue specificityi

    Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow.1 Publication

    Gene expression databases

    ArrayExpressiQ9NPF8.
    BgeeiQ9NPF8.
    CleanExiHS_ADAP2.
    GenevestigatoriQ9NPF8.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IKBKGQ9Y6K92EBI-718895,EBI-81279

    Protein-protein interaction databases

    BioGridi120915. 7 interactions.
    IntActiQ9NPF8. 3 interactions.
    MINTiMINT-1407535.
    STRINGi9606.ENSP00000329468.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NPF8.
    SMRiQ9NPF8. Positions 4-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 131123Arf-GAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini132 – 233102PH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini255 – 361107PH 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
    Contains 2 PH domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5347.
    HOGENOMiHOG000006719.
    HOVERGENiHBG050888.
    PhylomeDBiQ9NPF8.
    TreeFamiTF324540.

    Family and domain databases

    Gene3Di2.30.29.30. 2 hits.
    InterProiIPR001164. ArfGAP.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PfamiPF01412. ArfGap. 1 hit.
    [Graphical view]
    PRINTSiPR00405. REVINTRACTNG.
    SMARTiSM00105. ArfGap. 1 hit.
    SM00233. PH. 2 hits.
    [Graphical view]
    PROSITEiPS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NPF8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG    50
    VHRNFPDISR VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP 100
    QANDCLVLKE QWIRAKYERR EFMADGETIS LPGNREGFLW KRGRDNSQFL 150
    RRKFVLLARE GLLKYFTKEQ GKSPKAVISI KDLNATFQTE KIGHPHGLQI 200
    TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF PELPESELVP 250
    FLTRNYLKQG FMEKTGPKQK EPFKKRWFAL DCHERRLLYY KNPLDAFEQG 300
    QVFLGNKEQG YEAYEDLPKG IRGNRWKAGL TIVTPERRFV LTCPSEKEQQ 350
    EWLESLRGVL SSPLTPLNRL TASTESGRSS R 381
    Length:381
    Mass (Da):44,349
    Last modified:October 1, 2000 - v1
    Checksum:i4FAE208072A92C01
    GO
    Isoform 2 (identifier: Q9NPF8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         269-269: Missing.

    Note: May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:380
    Mass (Da):44,221
    Checksum:i86654BC0B1CECBF7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121L → P in CAC40651. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei269 – 2691Missing in isoform 2. 1 PublicationVSP_011180

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ238994 mRNA. Translation: CAB88383.1.
    AJ272195 mRNA. Translation: CAB77266.1.
    AJ242782 mRNA. Translation: CAC40651.1.
    BC033758 mRNA. Translation: AAH33758.1.
    CCDSiCCDS11261.1. [Q9NPF8-1]
    RefSeqiNP_060874.1. NM_018404.2. [Q9NPF8-1]
    UniGeneiHs.514063.

    Genome annotation databases

    EnsembliENST00000330889; ENSP00000329468; ENSG00000184060. [Q9NPF8-1]
    GeneIDi55803.
    KEGGihsa:55803.
    UCSCiuc002hfx.3. human. [Q9NPF8-1]
    uc002hfy.3. human. [Q9NPF8-2]

    Polymorphism databases

    DMDMi27923749.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ238994 mRNA. Translation: CAB88383.1 .
    AJ272195 mRNA. Translation: CAB77266.1 .
    AJ242782 mRNA. Translation: CAC40651.1 .
    BC033758 mRNA. Translation: AAH33758.1 .
    CCDSi CCDS11261.1. [Q9NPF8-1 ]
    RefSeqi NP_060874.1. NM_018404.2. [Q9NPF8-1 ]
    UniGenei Hs.514063.

    3D structure databases

    ProteinModelPortali Q9NPF8.
    SMRi Q9NPF8. Positions 4-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120915. 7 interactions.
    IntActi Q9NPF8. 3 interactions.
    MINTi MINT-1407535.
    STRINGi 9606.ENSP00000329468.

    PTM databases

    PhosphoSitei Q9NPF8.

    Polymorphism databases

    DMDMi 27923749.

    Proteomic databases

    MaxQBi Q9NPF8.
    PaxDbi Q9NPF8.
    PRIDEi Q9NPF8.

    Protocols and materials databases

    DNASUi 55803.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000330889 ; ENSP00000329468 ; ENSG00000184060 . [Q9NPF8-1 ]
    GeneIDi 55803.
    KEGGi hsa:55803.
    UCSCi uc002hfx.3. human. [Q9NPF8-1 ]
    uc002hfy.3. human. [Q9NPF8-2 ]

    Organism-specific databases

    CTDi 55803.
    GeneCardsi GC17P029248.
    HGNCi HGNC:16487. ADAP2.
    MIMi 608635. gene.
    neXtProti NX_Q9NPF8.
    PharmGKBi PA26405.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5347.
    HOGENOMi HOG000006719.
    HOVERGENi HBG050888.
    PhylomeDBi Q9NPF8.
    TreeFami TF324540.

    Miscellaneous databases

    GeneWikii CENTA2.
    GenomeRNAii 55803.
    NextBioi 60951.
    PROi Q9NPF8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NPF8.
    Bgeei Q9NPF8.
    CleanExi HS_ADAP2.
    Genevestigatori Q9NPF8.

    Family and domain databases

    Gene3Di 2.30.29.30. 2 hits.
    InterProi IPR001164. ArfGAP.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    Pfami PF01412. ArfGap. 1 hit.
    [Graphical view ]
    PRINTSi PR00405. REVINTRACTNG.
    SMARTi SM00105. ArfGap. 1 hit.
    SM00233. PH. 2 hits.
    [Graphical view ]
    PROSITEi PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of centaurin-alpha2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle."
      Whitley P., Gibbard A.M., Koumanov F., Oldfield S., Kilgour E.E., Prestwich G.D., Holman G.D.
      Eur. J. Cell Biol. 81:222-230(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions."
      Jenne D.E., Tinschert S., Stegmann E., Reimann H., Nuernberg P., Horn D., Naumann I., Buske A., Thiel G.
      Genomics 66:93-97(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. Bertsch U., Illies C., Mayr G.W.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Leukocyte.
    5. "Identification of gene structure and subcellular localization of human centaurin alpha 2, and p42IP4, a family of two highly homologous, Ins 1,3,4,5-P4-/PtdIns 3,4,5-P3-binding, adapter proteins."
      Hanck T., Stricker R., Sedehizade F., Reiser G.
      J. Neurochem. 88:326-336(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiADAP2_HUMAN
    AccessioniPrimary (citable) accession number: Q9NPF8
    Secondary accession number(s): Q8N4Q6, Q96SD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 27, 2003
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3